ORCID Profile
0000-0003-4497-7107
Current Organisations
University of Southern Denmark
,
Swiss Tropical and Public Health Institute
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Publisher: Public Library of Science (PLoS)
Date: 08-12-2021
DOI: 10.1371/JOURNAL.PBIO.3001483
Abstract: Cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA) signalling is essential for the proliferation of Plasmodium falciparum malaria blood stage parasites. The mechanisms regulating the activity of the catalytic subunit PfPKAc, however, are only partially understood, and PfPKAc function has not been investigated in gametocytes, the sexual blood stage forms that are essential for malaria transmission. By studying a conditional PfPKAc knockdown (cKD) mutant, we confirm the essential role for PfPKAc in erythrocyte invasion by merozoites and show that PfPKAc is involved in regulating gametocyte deformability. We furthermore demonstrate that overexpression of PfPKAc is lethal and kills parasites at the early phase of schizogony. Strikingly, whole genome sequencing (WGS) of parasite mutants selected to tolerate increased PfPKAc expression levels identified missense mutations exclusively in the gene encoding the parasite orthologue of 3-phosphoinositide–dependent protein kinase-1 (PfPDK1). Using targeted mutagenesis, we demonstrate that PfPDK1 is required to activate PfPKAc and that T189 in the PfPKAc activation loop is the crucial target residue in this process. In summary, our results corroborate the importance of tight regulation of PfPKA signalling for parasite survival and imply that PfPDK1 acts as a crucial upstream regulator in this pathway and potential new drug target.
Publisher: IOP Publishing
Date: 03-2020
DOI: 10.1088/1755-1315/451/1/012083
Abstract: Inappropriate urban flood management may cause deterioration of urban living quality, known as urban decay. In order to avoid this, the sensitivity of surface runoff parameters towards peak discharge (Q p ) and flood volume (V f ) needs to be analysed. By conducting sensitivity analysis, the influence of any runoff parameter to these two output can be assessed quantitatively. This study aims to sort out the sensitivity of seven runoff parameters towards Q p and V f at part of the suburb of Mawson Lakes, South Australia. Four synthetic rainfall events with the Annual Recurrence Interval (ARI) of 2-year and 50-year each with the duration of 12 hour and 72-hour were generated by the AusIFD software. The hourly intensity values of these synthetic rainfall were then fed into the Storm Water Management Model (SWMM) to predict Q p and V f . Further, the initial values of runoff parameters based on previous studies were used in the first flood estimation. Different sets of parameters which were 25% less than and greater than the initial values were used in the next flood modelling. Results showed that the most sensitive parameters were %-impervious followed by Manning’s n-pervious. Modelling using initial values of parameters produced Q p and V f of 0.340 m 3 /s and 2.372 MCM for 2-year ARI respectively, whereas the corresponding values for 50-year ARI were 0.519 m 3 /s and 3.424 MCM, respectively. Decreasing the parameter initial values by 25% produced. Meanwhile, increasing the parameters’ initial value by 25%.
Publisher: EDP Sciences
Date: 2018
DOI: 10.1051/MATECCONF/201819505002
Abstract: Sedimentation is an important issue in reservoir rehabilitation and maintenance. This problem is worsened by seasonal water scarcity as a characteristic of arid catchments. Worldwide loss of storage capacity due to sedimentation is much higher than the increase of capacity by the construction of new reservoirs. Further, sedimentation hinders the safe operation of not only shallow reservoirs but also deep reservoirs by means of turbidity currents which sporadically transport large volumes of sediments down to the dams. This study analyses the real case of Pengga reservoir and Batujai reservoir located in Central Indonesia. Selected sediment management alternatives were analysed and evaluated based on technical, social and environmental criteria. Evaluation of sediment management alternatives was conducted by utilising the REServoir CONservation (RESCON) tool. Results showed that, first, both flushing techniques and hydrosuction are feasible whereas dredging and trucking are not. Secondly, the flushing technique outweighs the Hydrosuction Sediment Removal (HSRS) method. Thirdly, application of the HSRS method in both reservoirs shares one thing in common, i.e. it requires an appropriate combination of the number of suction pipes as well as minimum suction pipe diameters. Lastly, there are some differences in the details of implementation of the flushing technique to both reservoirs.
Publisher: Universitas Mataram
Date: 27-12-2019
Abstract: PDSI is the drought index method which has good accuracy to be applied in Lombok Island. However, this method is only able to hindcast the drought without any procedure to predict the drought index in the future. So, this model aims to recognize the characteristics of drought in North Lombok for early mitigation and anticipating drought disasters purposes in this region. The results obtained from this study are that the drought pattern in North Lombok has the SARIMA model of (0,1,2) (0,1,1)12. The drought in North Lombok mainly occurs between May-October with an increasing of drought index tends for over last 20 years.
Publisher: IOP Publishing
Date: 04-2021
DOI: 10.1088/1755-1315/708/1/012034
Abstract: General guidance for reservoir operation can be referred to as the Standard Operation Procedure (SOP). Real circumstances such as drought, however, do not necessarily comply with these ideal assumptions, which then trigger the studies on optimal hedging rules. More frequent mild droughts are preferred to less frequent severe droughts. In the present work, the-constrained-based multi-objective optimization model was developed to optimize hedging rules for a reservoir located in Lombok Island, Central Indonesia. The rules were aimed to minimize two objective functions namely maximum Single Shortage (S S ) and Total Deficit (T D ). The former is a measure of reservoir operation during drought in short-term, whereas the latter is for long-term evaluation. Results show that these two measures are conflicting in nature, that is, the greater the S S , the smaller the T D and vice versa. The tradeoffs between S S and T D can be a helpful assist for water operators in executing their routine daily tasks by providing water allocation alternatives.
Publisher: Brawijaya University
Date: 30-11-2020
No related grants have been discovered for Beatriz Graça.