ORCID Profile
0000-0001-5263-9189
Current Organisations
Karolinska Institutet
,
Chinese University of Hong Kong
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Publisher: Wiley
Date: 14-06-2011
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D0QM01132K
Abstract: In this review, we introduce the recent progress on the employment of AIE-active materials for healthcare-related applications, such as clinical diagnosis, theragnosis, disease mechanism studies and personalized medicines.
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2SC01108E
Publisher: Wiley
Date: 28-04-2021
Publisher: Springer Science and Business Media LLC
Date: 05-08-2016
DOI: 10.1038/SREP30855
Abstract: Mitochondria and mitochondrial dynamics play vital roles in health and disease. With the intricate nanometer-scale structure and rapid dynamics of mitochondria, super-resolution microscopy techniques possess great un-tapped potential to significantly contribute to understanding mitochondrial biology and kinetics. Here we present a novel mitochondrial probe (MitoRed AIE) suitable for live mitochondrial dynamics imaging and single particle tracking (SPT), together with a multi-dimensional data analysis approach to assess local mitochondrial (membrane) fluidity. The MitoRed AIE probe localizes primarily to mitochondrial membranes, with 95 ms fluorophore on-time delivering 106 photons/ms, characteristics which we exploit to demonstrate live cell 100 fps 3D time-lapse tracking of mitochondria. Combining our experimental and analytical approaches, we uncover mitochondrial dynamics at unprecedented time scales. This approach opens up a new regime into high spatio-temporal resolution dynamics in many areas of mitochondrial biology.
Publisher: American Chemical Society (ACS)
Date: 03-06-2013
DOI: 10.1021/AM401486H
Abstract: 1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine (TCEP) were examined to obtain the optimal labeling condition. In addition to its specific labeling effect, molecule 2 also showed its advantage over traditional self-quenching dyes through labeling Cys containing BSA with different dye/Cys ratios.
Publisher: Wiley
Date: 11-05-2023
DOI: 10.1002/BTM2.10539
Abstract: Photodynamic therapy is becoming increasingly popular for combat of bacteria. In the clinical photodynamic combat of bacteria, one critical issue is to avoid the potential damage to the host since the reactive oxygen species produced by photosensitizers are also harmful to mammalian cells. In this work, we report an aggregation‐induced‐emission‐active bacterial inhibitor and photosensitizer, OEO‐TPE‐MEM (OTM), for the imaging, killing, and light‐enhanced inactivation of bacteria. OTM could efficiently bind to and kill Gram‐positive bacteria, while its affinity to Gram‐negative bacteria is lower, and a higher OTM concentration is required for killing Gram‐negative bacteria. OTM is also an efficient photosensitizer and could efficiently sensitize the production of reactive oxygen species, which enhances its killing effect on both Gram‐positive and Gram‐negative bacteria. More interestingly, OTM is very biocompatible with normal mammalian cells both in the dark and under light irradiation. OTM in mice models with bacteria‐infected wounds could promote the healing of infected wounds without affecting their organs and blood parameters, which makes it an excellent candidate for clinical applications.
Publisher: American Chemical Society (ACS)
Date: 10-01-2012
DOI: 10.1021/JA208720A
Abstract: Amyloid fibrillation of proteins is associated with a great variety of pathologic conditions. Development of new molecules that can monitor amyloidosis kinetics and inhibit fibril formation is of great diagnostic and therapeutic value. In this work, we have developed a biocompatible molecule that functions as an ex situ monitor and an in situ inhibitor for protein fibrillation, using insulin as a model protein. 1,2-Bis[4-(3-sulfonatopropoxyl)phenyl]-1,2-diphenylethene salt (BSPOTPE) is nonemissive when it is dissolved with native insulin in an incubation buffer but starts to fluoresce when it is mixed with preformed insulin fibril, enabling ex situ monitoring of amyloidogenesis kinetics and high-contrast fluorescence imaging of protein fibrils. Premixing BSPOTPE with insulin, on the other hand, inhibits the nucleation process and impedes the protofibril formation. Increasing the dose of BSPOTPE boosts its inhibitory potency. Theoretical modeling using molecular dynamics simulations and docking reveals that BSPOTPE is prone to binding to partially unfolded insulin through hydrophobic interaction of the phenyl rings of BSPOTPE with the exposed hydrophobic residues of insulin. Such binding is assumed to have stabilized the partially unfolded insulin and obstructed the formation of the critical oligomeric species in the protein fibrillogenesis process.
Publisher: Springer International Publishing
Date: 10-10-2018
Publisher: American Chemical Society (ACS)
Date: 20-11-2013
DOI: 10.1021/LA403720S
Abstract: The patterned honeycomb structure film with the aggregation-induced emission property was prepared successfully by the breath figure method and photopolymerization method. Characterization of the HeLa and HepG2 cell culture on this surface indicates the porous honeycomb structures show anticancer cells growth function. So this kind of honeycomb structure will be promising for the control of cancer cell growth behaviors and achieving the application of anticancer.
Publisher: Wiley
Date: 07-02-2019
Abstract: Protein is one of the four most important biomacromolecules in living systems. The detection, quantification, localization, and characterization of proteins is essential for an understanding of biological fundamentals, as well as for the diagnostics and treatment of protein-related diseases. By using intrinsic and extrinsic fluorescence, different techniques have been established to study proteins, many of which are now being routinely used in research laboratories and clinics. This review summarizes the applications of aggregation-induced emission (AIE) fluorescence in protein science. In contrast to traditional fluorescent dyes, the activation of AIE dyes is mainly attributed to the restriction of intramolecular motions. This unique turn-on mechanism of AIE dyes allows researchers to develop novel fluorogenic strategies for sensitive, selective, and reliable analysis of proteins. This review focuses on introducing AIE strategies for 1) detection, localization, and quantification of proteins 2) probing polymer conformational transitions of proteins 3) characterization of protein-ligand interactions and 4) evaluation of enzyme activities. Perspectives and challenges with respect to this emerging field of protein characterization are offered.
Publisher: Wiley
Date: 14-04-2018
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/C9NH00693A
Abstract: A novel nano-thermometer composed of butter and AIE molecules can be used for intracellular temperature mapping using fluorescence lifetime imaging.
Publisher: American Chemical Society (ACS)
Date: 22-05-2013
DOI: 10.1021/JA312581R
Abstract: There is a great demand for long-term cellular tracers because of their great importance in monitoring biological processes, pathological pathways, therapeutic effects, etc. Herein we report a new type of fluorescence "turn-on" probe for tracing live cells over a long period of time. We synthesized the fluorogenic probe by attaching a large number of tetraphenylethene (TPE) labels to a chitosan (CS) chain. The resultant TPE-CS bioconjugate shows a unique aggregation-induced emission (AIE) behavior. It is nonfluorescent when dissolved but becomes highly emissive when its molecules are aggregated. The AIE aggregates can be readily internalized by HeLa cells. The cellular staining by the TPE-CS aggregates is so indelible that it enables cell tracing for as long as 15 passages. The internalized AIE aggregates are kept inside the cellular compartments and do not contaminate other cell lines in the coculture systems, permitting the differentiation of specific cancerous cells from normal healthy cells.
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4CC03337J
Abstract: A tetraphenylethene-based caged compound is induced to emit strong cyan emission in aggregated state by UV irradiation. This property enables it to be applied in photo-patterning and anti-counterfeiting related areas.
Publisher: Wiley
Date: 17-04-2013
Abstract: The detection of nucleic acids, such as DNA and RNA, plays a significant role in genetic engineering, forensics, and bioinformatics. Traditional nucleic acid probes are mainly intercalators, which are potential mutagens, or groove binders that show high preference only for double-stranded DNA. We herein present two versatile fluorescent probes for nucleic acid detection and visualization. The nonemissive tetraphenylethene derivatives (TTAPE) are induced by DNA/RNA to emit, thereby showing a novel phenomenon of aggregation-induced emission (AIE). This kind of "light-up" property enables the quantitation and visualization of nucleic acids in aqueous solution and electrophoretic gels, respectively. The cationic TTAPE can penetrate cells with a compromised plasma membrane easily but cannot enter live cells with an intact membrane, thus making them useful for the differentiation between dead and live cells. On account of the high binding affinity to DNA, TTAPE can selectively label the chromosomes and nuclei in fixed cells, which provides a simple and fast method for the observation of cell mitosis. Owing to their AIE characteristics, the dye molecules aggregate in DNA-rich regions and exert appreciable quantum efficiency as well as superior photostability.
Publisher: Springer Science and Business Media LLC
Date: 11-01-2021
Publisher: Wiley
Date: 28-06-2014
Abstract: Long-term tracking of bacterial viability is of great importance for monitoring the viability change of bacteria under storage, evaluating disinfection efficiency, as well as for studying the pharmacokinetic and pharmacodynamic properties of antibacterials. Most of the conventional viability dyes, however, suffer from high toxicity and/or poor photostability, making them unsuitable for long-term studies. In this work, an aggregation-induced emission molecule, TPE-2BA, which can differentiate dead and living bacteria and serve as a highly fluorescent and photostable probe for long-term viability assay. TPE-2BA is a cell-impermeable DNA stain that binds to the groove of double-stranded DNA. Bacteria with compromised membrane open the access for TPE-2BA to reach DNA, endowing it with strong emission. The feasibility of using TPE-2BA for screening effective bactericides is also demonstrated. Plate count experiment reveals that TPE-2BA poses negligible toxicity to bacteria, indicating that it is an excellent probe for long-term bacterial viability assay.
Publisher: Proceedings of the National Academy of Sciences
Date: 20-05-2019
Abstract: Hydrogels maintain great potential for biomedical applications. However, predicting whether a chemical can form a hydrogel simply based on its chemical structure remains challenging. In this study, we developed a combinational approach to obtain a structurally erse hydrogel library with over 2,000 peptides as a training dataset for machine learning. We calculated their chemical features, including topological and physicochemical properties, and utilized machine learning methods to predict the self-assembly behavior.
Publisher: Royal Society of Chemistry (RSC)
Date: 16-07-2014
DOI: 10.1039/C4RA05765A
Publisher: Wiley
Date: 28-09-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4TC00923A
Abstract: The properties and potential applications of non-charged, water soluble poly(ethylene glycol)-decorated teraphenylethenes are investigated.
Publisher: Wiley
Date: 30-06-2021
Abstract: COVID‐19 pandemic, caused by severe acute respiratory syndrome coronavirus 2, has resulted in global social and economic disruption, putting the world economy to the largest global recession since the Great Depression. To control the spread of COVID‐19, cutting off the transmission route is a critical step. In this work, the efficient inactivation of human coronavirus with photodynamic therapy (PDT) by employing photosensitizers with aggregation‐induced emission characteristics (DTTPB) is reported. DTTPB is designed to bear a hydrophilic head and two hydrophobic tails, mimicking the structure of phospholipids on biological membranes. DTTPB demonstrates a broad absorption band covering the whole visible light range and high molar absorptivity, as well as excellent reactive oxygen species sensitizing ability, making it an excellent candidate for PDT. Besides, DTTPB can target membrane structure, and bind to the envelope of human coronaviruses. Upon light irradiation, DTTPB demonstrates highly effective antiviral behavior: human coronavirus treated with DTTPB and white‐light irradiation can be efficiently inactivated with complete loss of infectivity, as revealed by the significant decrease of virus RNA and proteins in host cells. Thus, DTTPB sensitized PDT can efficiently prevent the infection and the spread of human coronavirus, which provides a new avenue for photodynamic combating of COVID‐19.
Publisher: American Chemical Society (ACS)
Date: 23-08-2011
DOI: 10.1021/AM2009162
Abstract: Terpyridine-containing tetraphenylethenes (TPEs) are synthesized and their optical and metal sensing properties are investigated. They are practically nonluminescent in the solution state but become highly emissive as nanoparticle suspensions in poor solvents or thin films in the solid state, demonstrating a novel phenomenon of aggregation-induced emission (AIE). The emission of the nanoaggregates of TPEs is pH-sensitive: it is decreased and eventually quenched upon protonation of their terpyridine units because of their AIE nature. The TPEs can work as "turn-off" fluorescent chemosensors for metal ions and display different fluorescence responses to various metal ions. A characteristic red shift in the emission spectra is observed in the presence of Zn(2+), which facilitates the discrimination of Zn(2+) from other metal ions. Because of the metal-to-ligand-charge-transfer process, terpyridine-substituted TPEs display an obvious magenta color upon selectively binding with Fe(2+), allowing a rapid identification of Fe(2+) in the aqueous media by naked eyes.
Publisher: Wiley
Date: 17-08-2016
Publisher: American Chemical Society (ACS)
Date: 27-03-2015
DOI: 10.1021/AM509142K
Abstract: The rapid acquisition of antibiotic resistance poses difficulties in the development of effective methods to eliminate pathogenic bacteria. New bactericides, especially those do not induce the emergence of resistance, are thus in great demand. In this work, we report an aggregation-induced emission fluorogen, TPE-Bac, for bacterial imaging and elimination. TPE-Bac can be readily dissolved in aqueous solution with weak emission. The presence of bacteria can turn on its emission, and thus no washing step is required in the imaging process. Meanwhile, TPE-Bac can be applied as a bactericide for elimination of bacteria. The hiphilic TPE-Bac bearing two long alkyl chains and two positively charged amines can intercalate into the membrane of bacteria, increase membrane permeability and lead to dark toxicity. The efficiency of bacteria killing is greatly enhanced under light irradiation. TPE-Bac can serve as a photosensitizer to induce reactive oxygen species (ROS) generation, which ensures the efficient killing of bacteria. The TPE-Bac-containing agar plates can be continuously used for bacteria killing by applying light to induce ROS generation.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5CC04731E
Abstract: In this work, a red emission AIE active mitochondrial probe is developed. It is the first non-self-quenching mitochondria specific probe with membrane potential sensitivity. Its application in sensing the membrane potential differences in mouse sperm cells is demonstrated.
Publisher: American Association for the Advancement of Science (AAAS)
Date: 11-01-2023
Abstract: Cells are responsive to the mechanical environment, but the methods to detect simultaneously how different organelles react in mechanobiological processes remain largely unexplored. We herein report a dual organelle-targeting fluorescent probe, ( E )-1-[3-(diethoxyphosphoryl)propyl]-4-[4-(diethylamino)styryl]pyridin-1-ium bromide (ASP-PE), for mechanical mapping in live cells. ASP-PE is aggregation-induced emission active and is sensitive to the local mechanical environment. It targets the plasma membrane (PM) and intracellular mitochondria in cells by its phosphonate moiety and pyridinium. In this work, through ASP-PE staining, changes of membrane tension in the PM and mitochondria in response to varied osmotic pressure and substrate stiffness are visualized using fluorescence lifetime imaging microscopy. The mechanobiological importance of actin filaments and microtubules in the PM and mitochondria is also investigated using this probe. Computational simulations are applied to study the sensing mechanism of the probe. This study introduces a unique tool for mapping the membrane tension in the PM and mitochondria together, providing us great opportunities to study organelle’s interactions in mechanobiology.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5CC02486B
Abstract: A tetraphenylethene-based AIE bioprobe is developed for mitochondrial imaging. The probe shows high brightness, tolerance to environmental changes and photostability, making it promising for monitoring of mitophagy process.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5TB00458F
Abstract: Two azide-functionalized AIE fluorogens are synthesized and used for detecting S-phase DNA synthesis and cell proliferation based on EdU assay.
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1TC00604E
Abstract: A wavelength-switchable microlaser can be easily fabricated using environment-sensitive aggregation-induced emission luminogens (AIEgens) as the optical gain material.
Publisher: American Chemical Society (ACS)
Date: 27-12-2014
DOI: 10.1021/AC403616C
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C3SC50648G
Publisher: Wiley
Date: 10-01-2023
DOI: 10.1002/AGT2.312
Abstract: Rapid detection and quantification of outer membrane vesicle (OMV) are of both scientific value and clinical implications. However, limited tools are available for investigations of OMVs. Herein, we report a novel fluorescent probe with aggregation‐induced emission (AIE) characteristics, namely, OEO‐TPE‐MEM (OTM), for OMV detection. OTM emits faintly in an aqueous medium, but its fluorescence could be effectively turned on upon interacting with bacteria bodies and OMVs produced by Gram‐negative bacteria. Notably, OTM could provide quantitative information on bacterial membrane remodeling and OMV secretion and be applied to high‐throughput screening of OMV‐inducing agents. This study presents a powerful AIE probe for imaging and quantitative analysis of bacteria envelop and derived OMVs, which might be applied for evaluating research and clinical antimicrobial materials in future studies.
Publisher: American Chemical Society (ACS)
Date: 23-03-2022
Abstract: Microviscosity is a fundamental parameter in the biophysics of life science and governs numerous cellular processes. Thus, the development of real-time quantitative monitoring of microviscosity inside cells is important. The traditional probes for detecting microviscosity via time-resolved luminescence imaging (TRLI) are generally disturbed by autofluorescence or surrounding oxygen in cells. Herein, we developed loose packing nanoaggregates with aggregation-induced delayed fluorescence (FKP-POA and FKP-PTA) and free from the effect of oxygen and autofluorescence for viscosity mapping via TRLI. The feasibility of FKP-PTA nanoparticles (NPs) for microviscosity mapping through TRLI was demonstrated by monitoring the variation of microviscosity inside HepG2 cancer cells, which demonstrated a value change from 14.9 cP to 216.9 cP during the apoptosis. This indicates that FKP-PTA NP can be used as a probe for cellular microviscosity mapping to help people to understand the physiologically dynamic microenvironment. The present results are expected to promote the advancement of diagnostic and therapeutic methods to cope with related diseases.
Publisher: Chinese Chemical Society
Date: 14-07-2022
Publisher: Humana Press
Date: 2013
DOI: 10.1007/978-1-62703-336-7_16
Abstract: Fluorescence-based techniques have found wide applications in life science. Among various luminogenic materials, fluorescent nanoparticles have attracted much attention due to their fabulous emission properties and potential applications as sensors. Here, we describe the fabrication of fluorescent silica nanoparticles (FSNPs) containing aggregation-induced emission (AIE) luminogens. By employing surfactant-free sol-gel reaction, FSNPs with uniform size and high surface charge and colloidal stability are generated. The FSNPs emit strong light upon photoexcitation, due to the AIE characteristic of the silole -aggregates in the hybrid nanoparticles. The FSNPs are cytocompatible and can be utilized as fluorescent visualizer for intracellular imaging for HeLa cells.
Publisher: Wiley
Date: 08-02-2021
Publisher: Wiley
Date: 07-05-2022
Abstract: Dental caries is among the most prevalent dental diseases globally, which arises from the formation of microbial biofilm on teeth. Besides, tooth whitening represents one of the fastest‐growing areas of cosmetic dentistry. It will thus be great if tooth biofilm eradication can be combined with tooth whitening. Herein, a highly efficient photodynamic dental therapy strategy is reported for tooth biofilm eradication and tooth discoloration by employing a photosensitizer (DTTPB) with aggregation‐induced emission characteristics. DTTPB can efficiently inactivate S. mutans , and inhibit biofilm formation by suppressing the expression of genes associated with extracellular polymeric substance synthesis, bacterial adhesion, and superoxide reduction. Its inhibition performance can be further enhanced through combined treatment with chlorhexidine. Besides, DTTPB exhibits an excellent tooth‐discoloration effect on both colored saliva‐coated hydroxyapatite and clinical teeth, with short treatment time (less than 1 h), better tooth‐whitening performance than 30% hydrogen peroxide, and almost no damage to the teeth. DTTPB also demonstrates excellent biocompatibility with neglectable hemolysis effect on mouse red blood cells and almost no killing effect on mammalian cells, which enables its potential applications for simultaneous tooth biofilm eradication and tooth whitening in clinical dentistry.
Publisher: Wiley
Date: 06-04-2020
Publisher: MDPI AG
Date: 14-02-2018
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0CC06411D
Abstract: AIE-active molecular rotors for mitochondria imaging with improved uptake and retention in both live and fixed cells.
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2JM16613E
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C3TC31562B
Publisher: American Chemical Society (ACS)
Date: 11-08-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C4CC07911F
Abstract: Fluorescent probe, TPE-TPP, can differentiate monomeric, oligomeric and fibrillar α-synuclein which was previously difficult to achieve.
Publisher: Proceedings of the National Academy of Sciences
Date: 05-11-2021
Abstract: The high spatial resolution of three-dimensional (3D) fluorescence imaging of myelinated fibers will greatly facilitate the understanding of 3D neural networks and the pathophysiology of demyelinating diseases. However, existing myelin probes are far from satisfactory because of their low–signal-to-background ratio and poor tissue permeability. We herein developed a near-infrared aggregation-induced emission-active probe, PM-ML, for high-performance myelin imaging. PM-ML could specifically image myelinated fibers in teased sciatic nerves and mouse brain tissues with high contrast, good photostability, and deep penetration depth. PM-ML staining is compatible with several tissue-clearing methods. Its application in assessing myelination for neuropathological studies was also demonstrated using a multiple sclerosis mouse model.
Publisher: Wiley
Date: 23-07-2021
Abstract: Antibacterial photodynamic therapy (PDT) is one of the emerging methods for curbing multidrug‐resistant bacterial infections. Effective fluorescent photosensitizers with dual functions of bacteria imaging and PDT applications are highly desirable. In this study, three cationic and heteroleptic cyclometalated Ir(III) complexes with the formula of [Ir(CˆN) 2 (NˆN)][PF 6 ] are prepared and characterized. These Ir(III) complexes named Ir(ppy) 2 bP , Ir(1‐pq) 2 bP, and Ir(2‐pq) 2 bP are comprised of three CˆN ligands (i.e., 2‐phenylpyridine (ppy), 1‐phenylisoquinoline (1‐pq), and 2‐phenylquinoline (2‐pq)) and one NˆN bidentate co‐ligand (bP). The photophysical characterizations demonstrate that these Ir(III) complexes are red‐emitting, aggregation‐induced emission active luminogens. The substitution of phenylpyridine with phenylquinoline isomers in the molecules greatly enhances their UV and visible‐light absorbance as well as the photoinduced reactive oxygen species (ROS) generation ability. All three Ir(III) complexes can stain both Gram‐positive and Gram‐negative bacteria efficiently. Interestingly, even though Ir(1‐pq) 2 bP and Ir(2‐pq) 2 bP are constitutional isomers with very similar structures and similar ROS generation ability in buffer, the former eradicates bacteria much more effectively than the other through white light‐irradiated photodynamic inactivation. This work will provide valuable information on the rational design of Ir(III) complexes for fluorescence imaging and efficient photodynamic inactivation of bacteria.
Publisher: American Chemical Society (ACS)
Date: 26-03-2013
DOI: 10.1021/JA400337P
Abstract: Intracellular pH (pHi) is an important parameter associated with cellular behaviors and pathological conditions. Sensing pHi and monitoring its changes in live cells are essential but challenging due to the lack of effective probes. We herein report a pH-sensitive fluorogen for pHi sensing and tracking. The dye is a tetraphenylethene-cyanine adduct (TPE-Cy). It is biocompatible and cell-permeable. Upon diffusing into cells, it responds sensitively to pHi in the entire physiological range, visualizing the acidic and basic compartments with intense red and blue emissions, respectively. The ratiometric signal of the red and blue channels can thus serve as an indicator for local proton concentration. The utility of TPE-Cy in pHi imaging and monitoring is demonstrated with the use of confocal microscopy, ratiometric analysis, and flow cytometry.
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D1TB02524D
Abstract: This review focuses on recent advances in multifunctional high- Z nanomaterials for radiotherapeutic-based multimodal synergistic cancer therapy.
Publisher: Wiley
Date: 05-12-2019
Abstract: Self-assembled DNA origami nanostructures have a high degree of programmable spatial control that enables nanoscale molecular manipulations. A surface-tethered, flexible DNA nanomesh is reported herein which spontaneously undergoes sharp, dynamic conformational transitions under physiological conditions. The transitions occur between two major macrostates: a spread state dominated by the interaction between the DNA nanomesh and the BSA/streptavidin surface and a surface-avoiding contracted state. Due to a slow rate of stochastic transition events on the order of tens of minutes, the dynamic conformations of in idual structures can be detected in situ with DNA PAINT microscopy. Time series localization data with automated imaging processing to track the dynamically changing radial distribution of structural markers are combined. Conformational distributions of tethered structures in buffers with elevated pH exhibit a calcium-dependent domination of the spread state. This is likely due to electrostatic interactions between the structures and immobilized surface proteins (BSA and streptavidin). An interaction is observed in solution under similar buffer conditions with dynamic light scattering. Exchanging between solutions that promote one or the other state leads to in situ s le-wide transitions between the states. The technique herein can be a useful tool for dynamic control and observation of nanoscale interactions and spatial relationships.
Publisher: American Chemical Society (ACS)
Date: 20-12-2012
DOI: 10.1021/JA310324Q
Abstract: Tracking the dynamics of mitochondrial morphology has attracted much research interest because of its involvement in early stage apoptosis and degenerative conditions. To follow this process, highly specific and photostable fluorescent probes are in demand. Commercially available mitochondria trackers, however, suffer from poor photostability. To overcome this limitation, we have designed and synthesized a fluorescent agent, tetraphenylethene-triphenylphosphonium (TPE-TPP), for mitochondrial imaging. Inherent from the mitochondrial-targeting ability of TPP groups and the aggregation-induced emission (AIE) characteristics of the TPE core, TPE-TPP possesses high specificity to mitochondria, superior photostability, and appreciable tolerance to environmental change, allowing imaging and tracking of the mitochondrial morphological changes in a long period of time.
Publisher: American Chemical Society (ACS)
Date: 25-07-2019
Abstract: The analysis of albumin has clinical significance in diagnostic tests and obvious value to research studies on the albumin-mediated drug delivery and therapeutics. The present immunoassay, instrumental techniques, and colorimetric methods for albumin detection are either expensive, troublesome, or insensitive. Herein, a class of water-soluble tetrazolate-functionalized derivatives with aggregation-induced emission (AIE) characteristics is introduced as novel fluorescent probes for albumin detection. They can be selectively lighted up by site-specific binding with albumin. The resulting albumin fluorescent assay exhibits a low detection limit (0.21 nM), high robustness in aqueous buffer (pH = 6-9), and a broad tunable linear dynamic range (0.02-3000 mg/L) for quantification. The tetrazolate functionality endows the probes with a superior water solubility (>0.01 M) and a high binding affinity to albumin (
Publisher: CSIRO Publishing
Date: 2011
DOI: 10.1071/CH11170
Abstract: Tetraphenylethene derivatives [Ph(PhCH=CHPhR)C=C(PhCH=CHPhR)Ph, R=H, CN, NO2, NPh2] with green, yellow-green, and orange emission colours were designed and synthesized. These molecules are practically non-emissive in their dilute solutions but emit intensely as nanoaggregates in poor solvents, demonstrating a novel phenomenon of aggregation-induced emission. Their blended films with poly(methyl methacrylate) also display bright emissions. Restriction of intramolecular motion in the condensed phase may be responsible for such unusual behaviour. Multilayer electroluminescence devices with a configuration of indium tin oxide/N,N′-di(1-naphthyl)-N,N′-diphenylbenzidine/emitter/tris(8-hydroxyquinolinolato)aluminum (Alq3)/LiF/Al were constructed, which gave green light with a maximum luminance and current efficiency of 12930 cd cm–2 and 3.04 cd A–1 respectively. The tetraphenylethenes can serve as excellent cell staining agents for selectively illuminating the cytoplasm and vesicles of living cells.
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D0QM00914H
Abstract: A near-infrared AIE probe was developed for super-resolution imaging and nuclear lipid droplets dynamic study with good biocompatibility and high photostability.
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2JM32730A
Publisher: Wiley
Date: 20-12-2016
Abstract: In this work, a morpholine-functionalized aggregation-induced emission luminogen (AIEgen), AIE-LysoY, is reported for lysosomal imaging and autophagy visualization. To attain outstanding imaging contrast, AIE-LysoY is equipped with excited state intramolecular proton transfer (ESIPT) characteristic. AIE-LysoY provides a new platform for lysosome visualization with good biocompatibility, large Stokes shift, superior signal-to-noise ratio, and high photostability.
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2QM00630H
Abstract: This work presents the highly efficient photodynamic combat of viruses by employing three isoquinolinium-based photosensitizers with aggregation-induced emission characteristics.
Publisher: Wiley
Date: 02-02-2015
Abstract: Intracellular viscosity is a crucial parameter that indicates the functioning of cells. In this work, we demonstrate the utility of TPE-Cy, a cell-permeable dye with aggregation-induced emission (AIE) property, in mapping the viscosity inside live cells. Owing to the AIE characteristics, both the fluorescence intensity and lifetime of this dye are increased along with an increase in viscosity. Fluorescence lifetime imaging of live cells stained with TPE-Cy reveals that the lifetime in lipid droplets is much shorter than that from the general cytoplasmic region. The loose packing of the lipids in a lipid droplet results in low viscosity and thus shorter lifetime of TPE-Cy in this region. It demonstrates that the AIE dye could provide good resolution in intracellular viscosity sensing. This is also the first work in which AIE molecules are applied in fluorescence lifetime imaging and intracellular viscosity sensing.
Publisher: Hindawi Limited
Date: 2013
DOI: 10.1155/2013/853154
Abstract: We have fabricated the patterned porous honeycomb-like film with the aggregation-induced emission (AIE) property successfully by breath figure method. Characterization of contact angles (CA) indicated that the patterned porous honeycomb structure can improve the hydrophobicity of the film, while smooth films do not have this function. Characterization of the fluorescence property indicates that the patterned honeycomb structure films are highly emissive. This work could not only be promising for controlling molecular group reorientation and suitable for the application of manipulating surface composition of the film but also make the tetraphenylethene derivatives with AIE properties have a promising application future in special wettability surface. The present findings should open a way for the new application of honeycomb structure materials, which will be useful in many areas, such as sensors, tissue engineering, clinical medicine, and biomaterials.
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C2TB00155A
Publisher: American Chemical Society (ACS)
Date: 10-08-2022
DOI: 10.1021/JACS.2C07443
Abstract: Fluorescence imaging in the second near-infrared window (NIR-II, 1000-1700 nm) using small-molecule dyes has high potential for clinical use. However, many NIR-II dyes suffer from the emission quenching effect and extremely low quantum yields (QYs) in the practical usage forms. The AIE strategy has been successfully utilized to develop NIR-II dyes with donor-acceptor (D-A) structures with acceptable QYs in the aggregate state, but there is still large room for QY improvement. Here, we rationally designed a NIR-II emissive dye named TPE-BBT and its derivative (TPEO-BBT) by changing the electron-donating triphenylamine unit to tetraphenylethylene (TPE). Their nanoparticles exhibited ultrahigh relative QYs of 31.5% and 23.9% in water, respectively. By using an integrating sphere, the absolute QY of TPE-BBT nanoparticles was measured to be 1.8% in water. Its crystals showed an absolute QY of 10.4%, which is the highest value among organic small molecules reported so far. The optimized D-A interaction and the higher rigidity of TPE-BBT in the aggregate state are believed to be the two key factors for its ultrahigh QY. Finally, we utilized TPE-BBT for NIR-II photoluminescence (PL) and chemiluminescence (CL) bioimaging through successive CL resonance energy transfer and Förster resonance energy transfer processes. The ultrahigh QY of TPE-BBT realized an excellent PL imaging quality in mouse blood vessels and an excellent CL imaging quality in the local arthrosis inflammation in mice with a high signal-to-background ratio of 130. Thus, the design strategy presented here brings new possibilities for the development of bright NIR-II dyes and NIR-II bioimaging technologies.
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2CC33780K
Abstract: Melding a benzothiazolium unit with tetraphenylethene generates a new hemicyanine luminogen with aggregation-induced emission characteristics the luminogen exhibits crystochromism and its solid-state emission can be repeatedly tuned from yellow or orange to red by grinding-fuming or grinding-heating processes due to the transformation from the crystalline to the amorphous state and vice versa.
Publisher: Wiley
Date: 19-02-2019
Abstract: Phagocytosis of bacteria is an important biological process. Gaining insight into this process may greatly benefit related pathological studies and further contribute to development of therapies for infectious diseases. Tools for studying these internalization processes, however, are limited. Herein, we demonstrate the feasibility of employing an environmentally sensitive aggregation-induced emission (AIE) probe for bacteria labeling and imaging. By tracking the fluorescence variation of the stained bacteria, the pH changes of its microenvironment can be monitored. In this way, the phagocytic entry of these bacteria into the macrophage cells and the intravacuolar acidification can be visualized in real-time.
Publisher: Chinese Chemical Society
Date: 02-2022
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4CC07128J
Abstract: TPE-IQ, an AEE luminogen, can selectively illuminate mitochondria and serve as a photosensitizer to induce cell apoptosis upon photoexcitation. The mitochondrial-specificity increases its efficiency in photodynamic therapy.
Publisher: Wiley
Date: 06-11-2023
Abstract: Retinoblastoma (RB) is an aggressive eye cancer in infancy and childhood, lethal by metastasis if left untreated. Currently, the survival rate and the chance of saving vision depend on the severity of the disease. In this work, a highly efficient photodynamic ophthalmic therapy for RB is reported by employing an isoquinolinium‐based aggregation‐induced‐emission (AIE) photosensitizer (PS) TPE‐IQ‐2O for photodynamic inactivation (PDI). TPE‐IQ‐2O is an efficient mitochondria‐targeting photosensitizer as an efficient guided photodynamic therapy (PDT) agent against cancer cells. Maximizing cancer‐selectively damage to tumors with minimized side effects on normal tissue is essential for effective anticancer PDT and provides long‐lasting protection against metastasis. In addition, TPE‐IQ‐2O can effectively reduce the degree of tissue inflammation by inhibiting the expression of related inflammatory factors. TPE‐IQ‐2O also exhibits excellent biocompatibility with a neglectable hemolysis effect on mouse red blood cells and almost no killing effect on mammalian cells, which enables its potential applications in the treatment of RB.
Publisher: Wiley
Date: 22-09-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2JM31368E
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9CC07775H
Abstract: An aggregation-enhanced emission probe was developed for ultrafast labeling and high-fidelity imaging of mitochondria in cancer cells with a high signal-to-noise ratio.
Publisher: Wiley
Date: 14-07-2015
Abstract: A luminogen with aggregation-induced emission characteristics is reported for bacterial imaging and antibiotics screening studies. The luminogen can light up bacteria in a wash-free manner, which simplifies the imaging process and increases its accuracy in bacterial detection. It can also be applied to high-throughput screening of antibiotics and fast evaluation of bacterial susceptibility, giving reliable results in less than 5 h.
Publisher: Elsevier BV
Date: 02-2022
Publisher: Wiley
Date: 06-04-2020
Publisher: MyJove Corporation
Date: 21-04-2019
DOI: 10.3791/58669
Abstract: Silver staining is a colorimetric technique widely used to visualize protein bands in polyacrylamide gels following sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The classic silver stains have certain drawbacks, such as high background staining, poor protein recovery, low reproducibility, a narrow linear dynamic range for quantification, and limited compatibility with mass spectrometry (MS). Now, with the use of a fluorogenic Ag
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4TB00551A
Abstract: An aggregation-induced-emission active hemicyanine dye, TPE-Cy, shows high selectivity to homocysteine over glutathione and other amino acids.
Publisher: Wiley
Date: 14-04-2018
Publisher: Springer Science and Business Media LLC
Date: 07-03-2014
DOI: 10.1038/SREP04272
Publisher: Chinese Chemical Society
Date: 05-08-2022
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6MH00060F
Abstract: This review outlines the fabrication methods and bioimaging applications of the fluorescent nanoparticles based on AIE luminogens (AIE dots).
Publisher: Wiley
Date: 11-02-2013
Abstract: Chitosan with tetraphenylethene pendants (TPE-CS) are synthesized by reaction between amine and isothiocyanate groups of chitosan and tetraphenylethene (TPE), respectively. Nanoparticles of TPE-CS (TPE-CS NPs) are fabricated by ionic gelation method. The NPs are uniform in size, spherical in shape, monodispersed, and positive in surface charge. The suspension of TPE-CS NPs emits strong blue fluorescence under photoexcitation due to the aggregation-induced emission characteristics of the TPE moieties. The NPs can be internalized into cytoplasm through endocytosis pathway and retain inside the live cells to image the cells. Cytotoxicity assay reveals that TPE-CS NPs are cytocompatible and thus can be used for long-term live cell imaging.
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8MH00799C
Abstract: A new method using a single cell-permeable biochromic fluorescent dye to differentiate, quantify and image both live and dead cells is reported.
Location: Australia
No related grants have been discovered for Sijie Chen.