ORCID Profile
0000-0001-5668-0509
Current Organisation
UNSW Sydney
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Publisher: Hindawi Limited
Date: 2017
DOI: 10.1155/2017/4263762
Abstract: Biological self-assembly is a process in which building blocks autonomously organize to form stable supermolecules of higher order and complexity through domination of weak, noncovalent interactions. For silk protein, the effect of high incubating temperature on the induction of secondary structure and self-assembly was well investigated. However, the effect of freezing and thawing on silk solution has not been studied. The present work aimed to investigate a new all-aqueous process to form 3D porous silk fibroin matrices using a freezing-assisted self-assembly method. This study proposes an experimental investigation and optimization of environmental parameters for the self-assembly process such as freezing temperature, thawing process, and concentration of silk solution. The optical images demonstrated the possibility and potential of −80ST48 treatment to initialize the self-assembly of silk fibroin as well as controllably fabricate a porous scaffold. Moreover, the micrograph images illustrate the assembly of silk protein chain in 7 days under the treatment of −80ST48 process. The surface morphology characterization proved that this method could control the pore size of porous scaffolds by control of the concentration of silk solution. The animal test showed the support of silk scaffold for cell adhesion and proliferation, as well as the cell migration process in the 3D implantable scaffold.
Publisher: Elsevier BV
Date: 10-2019
DOI: 10.1016/J.MSEC.2019.04.049
Abstract: The purpose of this research is to investigate the effect of different oxidation degrees and volume ratios of components on the physical properties and biocompatibility of an in situ cross-linking chitosan-hyaluronic acid-based hydrogel for skin wound healing applications. Carboxymethyl groups (-CH
Publisher: Wiley
Date: 06-09-2929
Abstract: Human tissues and organs exhibit complex hierarchical and gradient structures that are essential to their function and should be recapitulated within biomaterial scaffolds targeting their regeneration. Unidirectional freezing, an ice templating technique where ice acts as a porogen, is uniquely suited to recapitulating the architectural anisotropy, gradients, and hierarchical transitions of human tissues, but ice templating of polymeric systems, including silk fibroin, remains less well understood than their colloidal counterparts. To address this, a versatile and accessible freezing setup for silk that allows tuning of freezing parameters including the polymer cooling rate (30 °C min −1 to 2 °C min −1 ) and ice solidification velocity (2.5 to 0.6 mm min −1 ) using liquid nitrogen, is developed. Real time visual and thermal monitoring of the freezing process for multiple silk concentrations (2–10% wt/v) and material states (liquid, hydrogels) is performed and the conditions are correlated with pore morphology. Unprecedented control over pore size (100–90 000 µm 2 ) and pore morphology (cellular–lamellar), consistent pore alignment, and generation of gradient porosity in silk scaffolds are demonstrated. For the first time, impact of shear thinning behavior of silk in ice crystal formation is demonstrated, showing non‐linear and complex freezing phenomena in silk.
Publisher: MDPI AG
Date: 23-11-2022
DOI: 10.3390/BIOENGINEERING9120722
Abstract: Type 1 diabetes results from the loss of pancreatic β cells, reduced insulin secretion and dysregulated blood glucose levels. Replacement of these lost β cells with stem cell-derived β cells, and protecting these cells within macro-device implants is a promising approach to restore glucose homeostasis. However, to achieve this goal of restoration of glucose balance requires work to optimise β cell function within implants. We know that native β cell function is enhanced by cell–cell and cell–extracellular matrix interactions within the islets of Langerhans. Reproducing these interactions in 2D, such as culture on matrix proteins, does enhance insulin secretion. However, the impact of matrix proteins on the 3D organoids that would be in implants has not been widely studied. Here, we use native β cells that are dispersed from islets and reaggregated into small spheroids. We show these β cell spheroids have enhanced glucose-dependent insulin secretion when embedded into softer alginate hydrogels conjugated with RGD peptide (a common motif in extracellular matrix proteins). Embedding into alginate–RGD causes activation of integrin responses and repositioning of liprin, a protein that controls insulin secretion. We conclude that insulin secretion from β cell spheroids can be enhanced through manipulation of the surrounding environment.
Publisher: Hindawi Limited
Date: 21-03-2019
DOI: 10.1155/2019/7382717
Abstract: Silver nanoparticles have attracted great interests widely in medicine due to its great characteristics of antibacterial activity. In this research, the antibacterial activity and biocompatibility of a topical gel synthesized from polyvinyl alcohol, chitosan, and silver nanoparticles were studied. Hydrogels with different concentrations of silver nanoparticles (15 ppm, 30 ppm, and 60 ppm) were evaluated to compare their antibacterial activity, nanoparticles’ sizes, and in vivo behaviors. The resulted silver nanoparticles in the hydrogel were characterized by TEM showing the nanoparticles’ sizes less than 22 nm. The in vitro results prove that the antibacterial effects of all of the s les are satisfied. However, the in vivo results demonstrate the significant difference among different hydrogels in wound healing, where hydrogel with 30 ppm shows the best healing rate.
Publisher: Elsevier BV
Date: 05-2023
Publisher: Elsevier BV
Date: 12-2020
Publisher: American Chemical Society (ACS)
Date: 27-08-2021
Abstract: Bacterial biofilms are indicated in most medical device-associated infections. Treating these biofilms is challenging yet critically important for applications such as in device-retention surgeries, which can have reinfection rates of up to 80%. This
Immobilization of Antimicrobial Silver and Antioxidant Flavonoid as a Coating for Wound Dressing Materials
Publisher: Informa UK Limited
Date: 12-2019
DOI: 10.2147/IJN.S230214
Publisher: Wiley
Date: 19-02-2023
Abstract: Three‐dimensional (3D) bioprinting technology offers great potential in the treatment of tissue and organ damage. Conventional approaches generally rely on a large form factor desktop bioprinter to create in vitro 3D living constructs before introducing them into the patient's body, which poses several drawbacks such as surface mismatches, structure damage, and high contamination along with tissue injury due to transport and large open‐field surgery. In situ bioprinting inside a living body is a potentially transformational solution as the body serves as an excellent bioreactor. This work introduces a multifunctional and flexible in situ 3D bioprinter (F3DB), which features a high degree of freedom soft printing head integrated into a flexible robotic arm to deliver multilayered biomaterials to internal organs/tissues. The device has a master‐slave architecture and is operated by a kinematic inversion model and learning‐based controllers. The 3D printing capabilities with different patterns, surfaces, and on a colon phantom are also tested with different composite hydrogels and biomaterials. The F3DB capability to perform endoscopic surgery is further demonstrated with fresh porcine tissue. The new system is expected to bridge a gap in the field of in situ bioprinting and support the future development of advanced endoscopic surgical robots.
Publisher: Elsevier BV
Date: 10-2020
Publisher: Informa UK Limited
Date: 11-2019
DOI: 10.2147/IJN.S219487
Publisher: MDPI AG
Date: 13-11-2020
DOI: 10.3390/NANO10112253
Abstract: Bacterial biofilms are involved in most device-associated infections and remain a challenge for modern medicine. One major approach to addressing this problem is to prevent the formation of biofilms using novel antimicrobial materials, device surface modification or local drug delivery however, successful preventive measures are still extremely limited. The other approach is concerned with treating biofilms that have already formed on the devices this approach is the focus of our manuscript. Treating biofilms associated with medical devices has unique challenges due to the biofilm’s extracellular polymer substance (EPS) and the biofilm bacteria’s resistance to most conventional antimicrobial agents. The treatment is further complicated by the fact that the treatment must be suitable for applying on devices surrounded by host tissue in many cases. Nanomaterials have been extensively investigated for preventing biofilm formation on medical devices, yet their applications in treating bacterial biofilm remains to be further investigated due to the fact that treating the biofilm bacteria and destroying the EPS are much more challenging than preventing adhesion of planktonic bacteria or inhibiting their surface colonization. In this highly focused review, we examined only studies that demonstrated successful EPS destruction and biofilm bacteria killing and provided in-depth description of the nanomaterials and the biofilm eradication efficacy, followed by discussion of key issues in this topic and suggestion for future development.
Publisher: Springer Singapore
Date: 24-09-2017
No related grants have been discovered for Hien Tran.