ORCID Profile
0000-0003-1463-6352
Current Organisation
Universitas Padjadjaran
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Maad Rayan Publishing Company
Date: 02-10-2020
DOI: 10.34172/PS.2020.63
Abstract: Background: Epithelial sodium channel (ENaC) is a transmembrane protein involved in maintaining sodium levels in blood plasma. It is also a potential biomarker for the early detection of hypertension since the amount of ENaC is related to the familial history of hypertension. ENaC can be detected by an aptamer, a single-stranded DNA (ssDNA) or RNA which offers advantages over an antibody. This study aimed to obtain an ssDNA aptamer specific to ENaC through virtual screening. Methods: Forty-one aptamers were retrieved from the Protein Data Bank (PDB) and the RNA was converted to ssDNA aptamers. The X-ray crystallographic structure of ENaC protein was remodelled using Modeller 9.20 to resolve missing residues. Molecular docking of aptamers against ENaC was performed using Patchdock and Firedock, then the selected aptamer was subjected to molecular docking against other ion channel proteins to assess its selectivity to ENaC. A molecular dynamics (MD) simulation was also conducted using Amber16 to acquire an in-depth understanding of the interaction within the aptamer-ENaC complex. Results: The virtual screening suggested that the ssDNA of iSpinach aptamer (PDB: 5OB3) displayed the strongest binding to ENaC (-49.46 kcal/mol) and was selective for ENaC over the other ion protein channels. An MMGBSA calculation on the complex of aptamer-ENaC revealed binding energy of -42,12 kcal/mol. Conclusion: The iSpinach-based aptamer is a potential probe for detecting ENaC or iDE and may be useful for the development of hypertension early detection systems.
Publisher: College of Science for Women
Date: 12-2020
DOI: 10.21123/BSJ.2020.17.4.1198
Abstract: This study was aimed to develop an optimized Dy determination method using differential pulse voltammetry (DPV). The Plackett-Burman (PB) experimental design was used to select significant factors that affect the electrical current response, which were further optimized using the response surface method-central composite design (RSM-CCD). The type of electrolyte solution and litude modulation were found as two most significant factors, among the nine factors tested, which enhance the current response based on PB design. Further optimization using RSM-CCD shows that the optimum values for the two factors were 0.1046 M and 0.1082 V respectively. When the optimum conditions were applied for Dy determination good recovery and precision were achieved with values of 91.58%, and 99.80%, respectively. The detection limit and quantification limit of the method were 1.4322 mg/L and of 4.7741 mg/L, respectively.
Publisher: IOP Publishing
Date: 02-2017
Publisher: The Royal Society
Date: 02-2021
DOI: 10.1098/RSOS.202040
Abstract: Epithelial sodium channel (ENaC) is a transmembrane protein that has an essential role in maintaining the levels of sodium in blood plasma. A person with a family history of hypertension has a high enough amount of ENaC protein in the kidneys or other organs, so that the ENaC protein acts as a marker that a person is susceptible to hypertension. An aptasensor involves aptamers, which are oligonucleotides that function similar to antibodies, as sensing elements. An electrochemical aptasensor for the detection of ENaC was developed using a screen-printed carbon electrode (SPCE) which was modified by electrodeposition of cerium oxide (CeO 2 ). The aptamer immobilization was via the streptavidin–biotin system. The measurement of changes in current of the active redox [Fe(CN) 6 ] 3−/4− was carried out by differential pulse voltammetry. The surfaces of SPCE and SPCE/CeO 2 were characterized using scanning electron microscopy, voltammetry and electrochemical impedance spectroscopy. The Box–Behnken experimental optimization design revealed the streptavidin incubation time, aptamer incubation time and streptavidin concentrations were 30 min, 30 min and 10.8 µg ml −1 , respectively. Various concentrations of ENaC were used to obtain the linearity range of 0.05–3.0 ng ml −1 , and the limits of detection and quantification were 0.012 ng ml −1 and 0.038 ng ml −1 , respectively. This aptasensor method has the potential to measure the ENaC protein levels in urine s les as well as to be a point-of-care device.
No related grants have been discovered for Yeni Wahyuni Hartati.