ORCID Profile
0000-0001-9494-4245
Current Organisations
The University of Texas at Austin
,
Cornell University
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Publisher: Cambridge University Press (CUP)
Date: 11-2007
DOI: 10.1017/S0007114507764747
Abstract: Consensus recommendations on behalf of the European Commission research projects Perinatal Lipid Metabolism (PeriLip www.perilip.org ) and Early Nutrition Programming (EARNEST www.metabolic-programming.org ), developed jointly with representatives of the Child Health Foundation (Stiftung Kindergesundheit www.kindergesundheit.de ), the Diabetic Pregnancy Study Group (DPSG www.medfak.uu.se/dpsg ), the European Association of Perinatal Medicine (EAPM www.europerinatal.com ), the European Society for Clinical Nutrition and Metabolism (ESPEN www.espen.org ), the European Society for Paediatric Gastroenterology, Hepatology and Nutrition, Committee on Nutrition (ESPGHAN www.espghan.org ), the International Federation of Placenta Associations (IFPA aculeate.hopto.org/IFPA ) and the International Society for the Study of Fatty Acids and Lipids (ISSFAL email www.issfal.org.uk ). Members of the Perinatal Lipid Intake Working Group Gioia Alvino, Juliana von Berlepsch, Hans Konrad Biesalski, Tom Clandinin, Hildegard Debertin a , Tamás Decsi, Hans Demmelmair a , Gernot Desoye bc , Veronika Dietz, Peter Dodds, Pauline Emmett, Fabio Facchinetti d , Matthew W. Gillman, Joachim Heinrich, Emilio Herrera b , Irene Hoesli, William C. Heird, Matthew Hyde, Kirsi Laitinen, John Laws, Elvira Larqué Daza, Iliana Lopez-Soldado, Maria Makrides, Kim Fleischer Michaelsen e , Sjurdur Olsen, Henar Ortega, Guy Putet, Imogen Rogers, Paola Roggero, Lubos Sobotka f , Hania Szajewska g , Hope Weiler. (Representing: a Child Health Foundation, b DPSG, c IFPA, d EAPM, e ISSFAL, f ESPEN, g ESPGHAN.) Dietary fat intake in pregnancy and lactation affects pregnancy outcomes and child growth, development and health. The European Commission charged the research project PERILIP, jointly with the Early Nutrition Programming Project, to develop recommendations on dietary fat intake in pregnancy and lactation. Literature reviews were performed and a consensus conference held with international experts in the field, including representatives of international scientific associations. The adopted conclusions include: dietary fat intake in pregnancy and lactation (energy%) should be as recommended for the general population pregnant and lactating women should aim to achieve an average dietary intake of at least 200 mg DHA/d intakes of up to 1 g/d DHA or 2·7 g/d n -3 long-chain PUFA have been used in randomized clinical trials without significant adverse effects women of childbearing age should aim to consume one to two portions of sea fish per week, including oily fish intake of the DHA precursor, α-linolenic acid, is far less effective with regard to DHA deposition in fetal brain than preformed DHA intake of fish or other sources of long-chain n -3 fatty acids results in a slightly longer pregnancy duration dietary inadequacies should be screened for during pregnancy and in idual counselling be offered if needed.
Publisher: Elsevier BV
Date: 12-2008
DOI: 10.1016/J.CHROMA.2008.10.045
Abstract: The applicability of comprehensive two-dimensional gas chromatography (GCxGC) for sterol analysis was investigated by separation and identification of endogenous sterols in standards, and spiked in human urine. The modulation temperature was optimized to achieve the best separation and signal enhancement. The separation pattern of trimethylsilyl (TMS) derivatives of sterols was compared on two complementary column sets. Whilst the BPX5/BPX50 column set offers better overall separation, BPX50/BPX5 provides better peak shape and sensitivity. Comparison of the identification power of GCxGC-TOFMS against both the NIST05 MS library and a laboratory created (in-house) TOFMS library was carried out on a free sterols extract of urine, derivatised and spiked at the World Anti-Doping Agency (WADA) limit of 2 ng mL(-1). The average match quality for 19 analysed sterols on the BPX50/BPX5 column set was 950/1000 when searched against the in-house library only four were identified against the NIST05 library, at a match threshold of 800. The match quality of GCxGC-TOFMS spectra was superior to that for analysis using 1D GC-TOFMS for sterols spiked in urine at 10 ng mL(-1). An r(2)>0.997 was obtained for the concentration range between 0.25 ng mL(-1) and 10 ng mL(-1) for three selected sterols. The lowest limit of detection (LOD) was obtained for estrone (0.1 ng mL(-1)) and the highest LOD was for 5alpha-androstan-3alpha,11beta-diol-17-one, epitestosterone and cholesteryl butyrate (1 ng mL(-1)), using a match threshold of at least 800 and signal-to-noise ratio of at least 10. TOFMS coupled to GCxGC enabled satisfactory identification of sterols in urine at their LOD. A minimum acceptable match (MAM) criterion for urinary sterols using 2D retention times and TOF mass spectra is introduced. This study shows that GCxGC-TOFMS yields high specificity for steroids derived from urine, with detection limits appropriate for use in doping control.
Publisher: American Chemical Society (ACS)
Date: 13-09-2022
DOI: 10.1021/ACS.ANALCHEM.2C00923
Abstract: Ru-based catalysis results in highly unsaturated fatty acid (HUFA) ethyl esters (EE) deuterated to various extents. The products carry
Publisher: Wiley
Date: 04-2005
DOI: 10.1007/S11745-006-1394-0
Abstract: Supercritical fluid extracts of New Zealand green-lipped mussels (NZGLM) have been suggested to have therapeutic properties related to their oil components. The large number of minor FA in NZGLM extract was characterized by a GC-CIMS/MS method that excels at identification of double-bond positions in FAME. The extract contained five major lipid classes: sterol esters, TAG, FFA, sterols, and polar lipids. The total FA content of the lipid extract was 0.664 g/mL. Fifty-three unsaturated FA (UFA) were fully identified, of which 37 were PUFA, and a further 21 UFA were detected for which concentrations were too low for assignment of double-bond positions. There were 17 saturated FA, with 14:0, 16:0, and 18:0 present in the greatest concentration. The 10 n-3 PUFA detected included 20:5n-3 and 22:6n-3, the two main n-3 FA n-3 PUFA at low concentrations were 18:3, 18:4, 20:3, 20:4, 21:5, 22:5, 24:6, and 28:8. There were 43 UFA from the n-4, n-5, n-6, n-7, n-8, n-9, n-10, n-1 families, with 16:2n-4, 16:1n-5, 18:1n-5, 18:2n-6, 20:4n-6, 16:1n-7, 20:1n-7, 16:1n-9, 18:1 n-9, and 20:1 n-9 being the most abundant. In general, we estimated that FAME concentrations greater than 0.05% (w/w) were sufficient to assign double-bond positions. In total, 91 FA were detected in an extract of the NZGLM, whereas previous studies of fresh flesh from the NZGLM had reported identification of 42 FA. These data demonstrate a remarkable ersity of NZGLM FA.
Publisher: Elsevier BV
Date: 08-2011
DOI: 10.1016/J.CHROMA.2011.06.014
Abstract: An alternative calibration procedure for the gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) measurements of the World Antidoping Agency (WADA) Accredited Laboratories is presented. To alleviate the need for externally calibrated CO₂ gas for GC-C-IRMS analysis of urinary steroid metabolites, calibration using an external standard mixture solution of steroids with certified isotopic composition was investigated. The reference steroids of the calibration mixture and routine s les underwent identical instrumental processes. The calibration standards bracketed the entire range of the relevant δ¹³C values for the endogenous and exogenous steroids as well as their chromatographic retention times. The certified δ¹³C values of the reference calibrators were plotted in relation to measured m/z ¹³CO₂/¹²CO₂ (i.e. R(45/44)) mass spectrometric signals of each calibrator. δ¹³C values of the s le steroids were calculated from the least squares fit through the calibration curve. The effect of the external calibration on δ¹³C values, using the same calibration standards and set of urine s les but different brands of GC-C-IRMS instruments, was assessed by an interlaboratory study in the WADA Accredited Laboratories of Sydney, Australia and Athens, Greece. Relative correspondence between the laboratories for determination of androsterone, etiocholanolone, 5β-androstane-3α,17β-diacetate, and pregnanediacetate means were SD(δ¹³C)=0.12‰, 0.58‰, -0.34‰, and -0.40‰, respectively. These data demonstrate that accurate intralaboratory external calibration with certified steroids provided by United States Antidoping Agency (USADA) and without external CO₂ calibration is feasible and directly applicable to the WADA Accredited Laboratories for the harmonization of the GC-C-IRMS measurements.
No related grants have been discovered for James Thomas Brenna.