ORCID Profile
0000-0002-3059-4503
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Publisher: Wiley
Date: 28-07-2022
Abstract: Conventional biosensors rely on the diffusion‐dominated transport of the target analyte to the sensor surface. Consequently, they require an incubation step that may take several hours to allow for the capture of analyte molecules by sensor biorecognition sites. This incubation step is a primary cause of long s le‐to‐result times. Here, alternating current electrothermal flow (ACET) is integrated in an organic electrochemical transistor (OECT)‐based sensor to accelerate the device operation. ACET is applied to the gate electrode functionalized with nanobody–SpyCatcher fusion proteins. Using the SARS‐CoV‐2 spike protein in human saliva as an ex le target, it is shown that ACET enables protein recognition within only 2 min of s le exposure, supporting its use in clinical practice. The ACET integrated sensor exhibits better selectivity, higher sensitivity, and lower limit of detection than the equivalent sensor with diffusion‐dominated operation. The performance of ACET integrated sensors is compared with two types of organic semiconductors in the channel and grounds for device‐to‐device variations are investigated. The results provide guidelines for the channel material choice in OECT‐based biochemical sensors, and demonstrate that ACET integration substantially decreases the detection speed while increasing the sensitivity and selectivity of transistor‐based sensors.
Publisher: American Chemical Society (ACS)
Date: 07-02-2023
Publisher: Springer Science and Business Media LLC
Date: 24-05-2021
Publisher: IEEE
Date: 06-03-2022
Publisher: IEEE
Date: 10-07-2022
Publisher: Cold Spring Harbor Laboratory
Date: 13-11-2020
DOI: 10.1101/2020.11.12.20228874
Abstract: The COVID-19 pandemic highlights the need for rapid protein detection and quantification at the single-molecule level in a format that is simple and robust enough for widespread point-of-care applications. We here introduce a modular nanobody-organic electrochemical transistor architecture that enables the fast and specific detection and quantification of single-molecule to nanomolar protein antigen concentrations in complex bodily fluids. The sensor combines a new solution-processable organic semiconductor material in the transistor channel with the high-density and orientation-controlled bioconjugation of nanobody fusion proteins on disposable gate electrodes. It provides results after a 10 minutes exposure to 5 µL of unprocessed s les, maintains high specificity and single-molecule sensitivity in human saliva or serum, and is rapidly reprogrammed towards any protein target for which nanobodies exist. We demonstrate the use of this highly modular platform for the detection of green fluorescent protein, SARS-CoV-1/2, and MERS-CoV spike proteins and validate the sensor for COVID-19 screening in unprocessed clinical nasopharyngeal swab and saliva s les.
Publisher: Wiley
Date: 25-08-2019
Location: Saudi Arabia
No related grants have been discovered for Shofarul Wustoni.