ORCID Profile
0000-0002-8607-7871
Current Organisation
RMIT University
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Publisher: The Optical Society
Date: 06-08-2019
DOI: 10.1364/OE.27.023919
Publisher: Wiley
Date: 21-01-2016
Abstract: Dielectrophoresis is a widely used means of manipulating suspended particles within microfluidic systems. In order to efficiently design such systems for a desired application, various numerical methods exist that enable particle trajectory plotting in two or three dimensions based on the interplay of hydrodynamic and dielectrophoretic forces. While various models are described in the literature, few are capable of modeling interactions between particles as well as their surrounding environment as these interactions are complex, multifaceted, and computationally expensive to the point of being prohibitive when considering a large number of particles. In this paper, we present a numerical model designed to enable spatial analysis of the physical effects exerted upon particles within microfluidic systems employing dielectrophoresis. The model presents a means of approximating the effects of the presence of large numbers of particles through dynamically adjusting hydrodynamic drag force based on particle density, thereby introducing a measure of emulated particle-particle and particle-liquid interactions. This model is referred to as "dynamic drag force based on iterative density mapping." The resultant numerical model is used to simulate and predict particle trajectory and velocity profiles within a microfluidic system incorporating curved dielectrophoretic microelectrodes. The simulated data are compared favorably with experimental data gathered using microparticle image velocimetry, and is contrasted against simulated data generated using traditional "effective moment Stokes-drag method," showing more accurate particle velocity profiles for areas of high particle density.
Publisher: Wiley
Date: 10-09-2019
Publisher: The Optical Society
Date: 16-07-2019
DOI: 10.1364/OE.27.021532
Publisher: OSA
Date: 2019
Publisher: SPIE
Date: 30-12-2019
DOI: 10.1117/12.2541248
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6RA20688C
Abstract: We report a novel injection moulding technique for fabrication of complex multi-layer microfluidic structures, allowing one-step robust integration of functional components with microfluidic channels and fabrication of elastomeric valves.
Publisher: OSA
Date: 2018
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C7LC01320E
Abstract: Using a battery of biological and haemodynamic testing we identify a pneumatic microvalve geometry with optimised haemocompatibility.
Publisher: Springer Science and Business Media LLC
Date: 21-11-2017
DOI: 10.1038/S41598-017-16276-7
Abstract: Localized Ca 2+ influx via TRPV4 on the surface of endothelial cells greatly influences endothelial adaptation to blood flow, but how mechanical stress from blood flow controls TRPV4 integration into this physiological function is not fully understood. Here, we studied the spatial organization of TRPV4 and its relationship to the adherens junction component β-catenin using single- and dual-color direct stochastic optical reconstruction microscopy (dSTORM). In non-stimulated endothelial cells, TRPV4 is clustered in small protein islands, as is β-catenin. Using dual-color imaging, we found that TRPV4 and β-catenin reside in similar islands and can be found at both the basolateral and basal membranes. Following shear stress stimulation, TRPV4 molecules formed smaller clusters, with the majority residing outside of clusters. Further shear stress stimulation changed the molecular distribution of TRPV4 molecules, limiting them to the basal membrane. This redistribution and the smaller clusters resulted in the segregation of TRPV4 from β-catenin. Furthermore, TRPV4 trafficking was controlled by focal adhesion kinase and activation of the α5ß1 integrin. These highly differentiated spatial redistributions suggest that mechanotransduction of blood flow is controlled via a more complex hierarchy than previously thought.
Publisher: Portico
Date: 31-01-2015
Abstract: Lab-on-a-chip based portable blood analysis systems would allow point-of-care measurements, e.g. in an ambulance, or in remote areas with no fast access to medical care. Such a systemwould provide much faster information about the health of a patient. Here,we present a system that is based on absorption spectroscopy and uses an organic laser, which is tunable in the visible range. The feasibility of the system is shown with a table-top setup using laboratory equipment. Measurements of human albumin show linear behaviour in a range from 2.5 g/L to 60 g/L. In a consecutive setup the system is implemented on a microfluidic chip and is capable of measuring simultaneously transmitted and side scattered intensities, even with ambient light present. Air-suspended grating couplers on polymers are shown as the first element of a lab-on-a-chip implementation.
Publisher: OSA
Date: 2019
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7LC00524E
Abstract: This work presents an on-chip valve-based microfluidic automation module, capable of performing the complex fluid handling required for photonic biosensors.
No related grants have been discovered for Markus Knoerzer.