ORCID Profile
0000-0002-0837-0351
Current Organisations
Scuola Normale Superiore
,
Universita degli Studi di Bologna
,
University of Nottingham
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Publisher: Wiley
Date: 12-2007
Abstract: Glycopolymers have been synthesised by post‐functionalisation of well‐defined alkyne‐functional polymers with sugar azides to yield N ‐glycosyl 1,2,3‐triazole functional polymers. The Cu(I)‐catalysed Huisgen cycloaddition was used to attach α‐mannoside, β‐galactoside and β‐lactoside derivatives via an azide functionality bound directly to the sugar anomeric carbon. Three different catalytic systems were investigated for the click reactions [(PPh 3 ) 3 Cu(I)Br], TBTA/Cu(I)Br and bathophenanthrolinedisulphonic acid disodium salt/Cu(I)Br. The latter of these was found to be the most efficient for the attachment of the larger/more sterically hindered disaccharide lactose moiety. The interaction of the lactose‐ and galactose‐bearing glycopolymers with Ricinus Communis Agglutinin (RCA I) lectin was investigated by affinity HPLC analysis. The rate of the interaction between mannose polymer and concanavalin A (Con A) lectin was assessed by turbidimetry. The results from the lectin conjugation studies indicate that the glycopolymers prepared in this work are able to function as multivalent ligands, further suggesting that the attachment of the triazole directly to the sugar anomeric carbon has no significant effect on the interaction of these glycopolymers with Con A and RCA I.
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C3PY00744H
Publisher: Royal Society of Chemistry (RSC)
Date: 2006
DOI: 10.1039/B609065F
Publisher: American Chemical Society (ACS)
Date: 17-11-2007
DOI: 10.1021/JA072999X
Publisher: Royal Society of Chemistry (RSC)
Date: 2007
DOI: 10.1039/B618325E
Publisher: American Chemical Society (ACS)
Date: 15-09-2007
DOI: 10.1021/MA071362V
Publisher: American Chemical Society (ACS)
Date: 02-06-2015
DOI: 10.1021/ACS.BIOMAC.5B00286
Abstract: New pH-responsive polymersomes for active anticancer oligonucleotide delivery were prepared from triblock copolymers. The delivery systems were formed by two terminal hydrophilic blocks, PEG and polyglycerolmethacrylate (poly-GMA), and a central weakly basic block, polyimidazole-hexyl methacrylate (poly-ImHeMA), which can complex with oligonucleotides and control vesicle formation/disassembly via pH variations. Targeted polymersomes were prepared by mixing folate-derivatized and underivatized copolymers. At pH 5, ds-DNA was found to complex with the pH-responsive copolymers at a N/P molar ratio above ∼2:1, which assisted the encapsulation of ds-DNA in the polymersomes, while low association was observed at pH 7.4. Cytotoxicity studies performed on folate receptor overexpressing KB and B16-F10 cells and low folate receptor expressing MCF-7 cells showed high tolerance of the polymersomes at up to 3 mg/mL concentration. Studies performed with red blood cells showed that at pH 5.0 the polymersomes have endosomolytic properties. Cytofluorimetric studies showed a 5.5-fold higher uptake of ds-DNA loaded folate-functional polymersomes in KB cells compared to nontargeted polymersomes. In addition, ds-DNA was found to be localized both in the nucleus and in the cytosol. The incubation of luciferase transfected B16-F10 cells with targeted polymersomes loaded with luciferase and Hsp90 expression silencing siRNAs yielded 31 and 23% knockdown in target protein expression, respectively.
Publisher: Elsevier BV
Date: 04-2016
Publisher: American Chemical Society (ACS)
Date: 25-09-2004
DOI: 10.1021/JA0456454
Abstract: Application of proteins and peptides as human therapeutics is expanding rapidly as drug discovery becomes more prevalent. Conjugation of polymers to proteins can circumvent many problems and pegylation of proteins is now emerging as acceptable practice. This paper describes the synthesis of alpha-aldehyde-terminated poly(methoxyPEG)methacrylates from Cu(I) mediated living radical polymerization (Mn = 11 000, 22 000 and 32 000 PDi < 1.15), and their efficient conjugation to lysozyme, as a model protein. This offers an attractive and flexible alternative to linear poly(ethylene glycol) opening up the possibility of using the full power of living radical polymerization.
Publisher: Royal Society of Chemistry (RSC)
Date: 2007
DOI: 10.1039/B709080C
Abstract: The biotin-terminated PAMAM dendron has been synthesized and the asymmetric dendron used to modify the protein avidin via non-covalent bioconjugation.
Publisher: American Chemical Society (ACS)
Date: 12-02-2005
DOI: 10.1021/JA0430999
Abstract: A series of alpha-functional maleimide polymethacrylates (M(n) = 4.1-35.4 kDa, PDi = 1.06-1.27) have been prepared via copper-catalyzed living radical polymerization (LRP). Two independent synthetic protocols have been successfully developed and the polymers obtained in multigram scale, with an 80-100% content of maleimide reactive chain ends, depending on the method employed. A method for the synthesis of amino-terminated polymers, starting from Boc-protected amino initiators, has also been developed, as these derivatives are key intermediates in one of the two processes studied in the present work. The alternative synthetic pathway involves an initiator containing a maleimide unit "protected" as a Diels-Alder adduct. After the polymerization step, the maleimide functionality has been reintroduced by retro-Diels-Alder reaction, by simply refluxing those polymers in toluene for 7 h. These maleimido-terminated materials, poly(methoxyPEG((475))) methacrylates and poly(glycerol) methacrylates, differ for both the nature and size of the polymer side branches and showed an excellent solubility in water, a property that made them an ideal candidate for the synthesis of new polymer-(poly)peptide biomaterials. These functional polymers have been successfully employed in conjugation reactions in the presence of thiol-containing model substrates, namely, reduced glutathione (gamma-Glu-Cys-Gly) and the carrier protein, bovine serum albumin (BSA), in 100 mM phosphate buffer (pH 6.8-7.4) and ambient temperature.
Publisher: Royal Society of Chemistry (RSC)
Date: 2005
DOI: 10.1039/B500558B
Abstract: Azide terminally functional poly(methyl methacrylate)s (Mn = 4000-6000, PDI = 1.21-1.28) have been prepared by living radical polymerization and successfully reacted with alkynes in a Huisgen cycloaddition (click) reaction in one pot using the same catalyst for both processes.
Publisher: Royal Society of Chemistry (RSC)
Date: 2008
DOI: 10.1039/B718112D
Abstract: Polymers containing poly(ethylene glycol) methacrylate and 2-(2-methoxyethoxy)ethyl methacrylate have been synthesized by Cu(0)-mediated radical polymerisation for use as thermoresponsive water-dispersants for carbon nanotubes.
Publisher: Royal Society of Chemistry (RSC)
Date: 2004
DOI: 10.1039/B407712A
Abstract: The synthesis of protein-polymer bioconjugates is reported using N-succinimidyl ester functionalised polymers from transition metal mediated living radical polymerisation.
Publisher: Wiley
Date: 08-07-2016
DOI: 10.1002/POLA.28215
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Giuseppe Mantovani.