Renier van der Hoorn

Classification and Nomenclature of Metacaspases and Paracaspases: No More Confusion with Caspases

Publisher: Elsevier BV

Date: 03-2020

DOI: 10.1016/J.MOLCEL.2019.12.020

Major Cys protease activities are not essential for senescence in individually darkened Arabidopsis leaves

Publisher: Springer Science and Business Media LLC

Date: 06-01-2017

DOI: 10.1186/S12870-016-0955-5

Enhanced late blight resistance by engineering an EpiC2B‐insensitive immune protease

Publisher: Wiley

Date: 30-10-2023

DOI: 10.1111/PBI.14209

Activity‐based protein profiling of hydrolytic enzymes induced by gibberellic acid in isolated aleurone layers of malting barley

Publisher: Wiley

Date: 04-08-2016

DOI: 10.1002/1873-3468.12320

Abstract: During barley germination, the aleurone layer secretes most of the enzymes required to degrade the endosperm, many of which are yet to be characterized. We used activity-based protein profiling (ABPP) to detect a range of active enzymes extracted from aleurone layers isolated from grains of a commercial malting barley variety incubated with or without gibberellic acid (GA). Enzymes found to be induced by GA were putative aleurains, cathepsin-B-like proteases and serine hydrolases. By using an inhibitory sugar panel, a specific active retaining β-glycosidase in the barley aleurone was identified as a putative xylanase. Our results show that ABPP can be used rapidly to identify a variety of active enzyme isoforms in cereal aleurone without the need for enzyme purification.

Enhanced late blight resistance by engineering an EpiC2B-insensitive immune protease

Publisher: Cold Spring Harbor Laboratory

Date: 29-05-2023

DOI: 10.1101/2023.05.29.541874

Abstract: Crop protection strategies relying on the improvement of the natural plant immune system via genetic engineering are sustainable solutions against the pathogen thread on food security. Here we describe a novel way to improve the plant immune system by immune protease engineering. As proof of concept, we increased resistance against the late blight pathogen Phytopththora infestans by rendering the tomato secreted immune protease Pip1 insensitive to the P. infestans -secreted inhibitor Epic2B. This concept can be applied to secreted immune proteases in crops by precision breeding.

University Of Oxford

Location: United Kingdom of Great Britain and Northern Ireland

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Discovery Projects - Grant ID: DP190102058

Start Date: 2019

End Date: 12-2021

Amount: $422,000.00

Funder: Australian Research Council

Discovery Projects - Grant ID: DP180103377

Start Date: 04-2018

End Date: 03-2024

Amount: $485,806.00

Funder: Australian Research Council