ORCID Profile
0000-0002-5763-9767
Current Organisation
The University of Auckland
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Publisher: Elsevier BV
Date: 2013
DOI: 10.1016/J.YMPEV.2012.09.027
Abstract: The origins and evolutionary history of the New Zealand flora has been the subject of much debate. The recent description of Cyathodophyllum novaezelandieae from early Miocene sediments in New Zealand provides possible evidence for the antiquity of the fleshy fruited epacrids (tribe Styphelieae, Ericaceae) in New Zealand. Yet the extant species in this tribe are thought to be very closely related to or conspecific with Australian taxa, suggesting recent trans-Tasman origins. In order to investigate the origins and evolution of the extant New Zealand Styphelieae we produced molecular phylogenetic trees based on sequences of three plastid regions that include representatives of all the genera of the tribe and eight of the ten New Zealand species. We estimated the range of minimum ages of the New Zealand lineages with Bayesian relaxed-clock analyses using different calibration methods and relative dating. We found strong support for each of the eight extant species of New Zealand Styphelieae being a distinct lineage that is nested within an Australian clade. In all except one case the sister is from Tasmania and/or the east coast of mainland Australia for Acrothamnus colensoi the sister is in New Guinea. Estimated dates indicate that all of the New Zealand lineages erged from their non-New Zealand sisters within the last 7 Ma. Time discontinuity between the fossil C.novae-zelandiae (20-23 Ma) and the origins of the extant New Zealand lineages (none older than 5 Ma) indicates that the fossil and extant Styphelieae in New Zealand are not related. The relative dating analysis showed that to accept this relationship, it would be necessary to accept that the Styphelieae arose in the early-mid Mesozoic (210-120 Ma), which is starkly at odds with multiple lines of evidence on the age of Ericales and indeed the angiosperms. Therefore, our results do not support the hypothesis that Styphelieae have been continuously present in New Zealand since the early Miocene. Instead they suggest a historical biogeographical scenario in which the lineage to which C. novae-zelandiae belongs went extinct in New Zealand, and the extant New Zealand Styphelieae are derived from Australian lineages that recolonised (presumably by long distance dispersal) no earlier than the late Miocene to Pliocene.
Publisher: CSIRO Publishing
Date: 2012
DOI: 10.1071/SB11033
Abstract: Leucopogon extremus Hislop & Puente-Lelievre, a new species of Leucopogon s. str. with a remarkably anomalous morphology, is described, illustrated and its distribution mapped. Its placement within the genus is confirmed through phylogenetic analyses of the plastid rbcL, matK, atpB–rbcL and trnH–psbA sequences.
Publisher: CSIRO Publishing
Date: 2020
DOI: 10.1071/SB18050
Abstract: The taxonomic limits of Styphelia (Ericaceae, Epacridoideae, Styphelieae) have been contentious since the genus was first described. At one extreme, it has been circumscribed so broadly as to include most epacrids with drupaceous fruit, at the other, to include only those species that also have long-exserted anthers and styles. Recent molecular phylogenetic analyses have indicated that while all previous circumscriptions of Styphelia are non-monophyletic, a large clade (the Astroloma–Styphelia clade) is consistently well supported. This clade comprises Astroloma, in part (i.e. section Stomarrhena sensu Bentham), Coleanthera, Croninia, Leucopogon, in part (i.e. section Pleuranthus sensu Bentham) and Styphelia sensu Bentham. On the basis of those analyses, we here recircumscribe Styphelia phylogenetically to include all species belonging to the Styphelia–Astroloma clade. The 146 taxa occur mostly in Australia, with smaller numbers in New Zealand, New Caledonia (1 species extends to Fiji and Vanuatu) and Malesia. An additional 74 phrase-named taxa belong to this clade, including 70 from Western Australia and 4 from eastern Australia (all other Australian states and territories). The Styphelia floras of Western Australia, eastern Australia, New Caledonia and Malesia are each endemic or nearly so 1 species (S. nesophila (DC.) Sleumer) is shared between New Zealand and eastern Australia, and 2 species (S. cordifolia (Lindl.) F.Muell. and S. woodsii (F.Muell.) F.Muell.) are shared between Western Australia and eastern Australia. An amended diagnosis of Styphelia is provided, new combinations are made for 25 taxa, and new names published for another 9. Lectotypes are designated for two names (Leucopogon brevicuspis Benth. and L. strictus Benth.) found to have taxonomically heterogeneous syntypes.
Publisher: American Chemical Society (ACS)
Date: 03-08-2018
Abstract: Peanut is one of the most commonly consumed allergy-causing foods in the United States. Prevention of accidental consumption by allergic in iduals is assisted by methods that effectively identify the presence of peanut in food, even at trace levels. This study presents a multiplex real-time polymerase chain reaction (PCR) assay that uses chloroplast markers ( matK, rpl16, and trnH-psbA) to specifically detect peanut in three types of foods: baked goods, chocolate, and tomato sauces. Food matrices were spiked with raw peanut at concentrations ranging from 0.1 to 10
Publisher: Elsevier BV
Date: 03-2021
Publisher: Springer Science and Business Media LLC
Date: 26-05-2020
DOI: 10.1038/S41598-020-64919-Z
Abstract: DNA barcoding is a valuable tool to support species identification with broad applications from traditional taxonomy, ecology, forensics, food analysis, and environmental science. We introduce Microfluidic Enrichment Barcoding (MEBarcoding) for plant DNA Barcoding, a cost-effective method for high-throughput DNA barcoding. MEBarcoding uses the Fluidigm Access Array to simultaneously lify targeted regions for 48 DNA s les and hundreds of PCR primer pairs (producing up to 23,040 PCR products) during a single thermal cycling protocol. As a proof of concept, we developed a microfluidic PCR workflow using the Fluidigm Access Array and Illumina MiSeq. We tested 96 s les for each of the four primary DNA barcode loci in plants: rbcL, matK, trnH-psbA , and ITS. This workflow was used to build a reference library for 78 families and 96 genera from all major plant lineages – many currently lacking in public databases. Our results show that this technique is an efficient alternative to traditional PCR and Sanger sequencing to generate large amounts of plant DNA barcodes and build more comprehensive barcode databases.
Publisher: CSIRO Publishing
Date: 2019
DOI: 10.1071/SB18026
Abstract: Styphelia geniculata Crayn and S. piliflora Crayn are described, phylogenetically placed and compared with related species. Their relationships within Ericaceae tribe Styphelieae, resolved by phylogenetic analysis of plastid and nuclear DNA sequences, support their description in the expanded genus Styphelia. Illustrations, pollen scanning electron micrographs, distribution maps and a key to the species and related northern Queensland taxa are provided, along with notes on the ecology and conservation status.
Publisher: CSIRO Publishing
Date: 2016
DOI: 10.1071/SB14041
Abstract: The Styphelieae is the largest of the seven tribes within the subfamily Epacridoideae Arn. (Ericaceae Juss.). Recent molecular phylogenetic work has resulted in the recircumscription of some genera and the erection of new ones, but several non-monophyletic genera remain. Most of them are concentrated in the well-supported Styphelia–Astroloma clade, which contains species currently assigned to Leucopogon R.Br., Styphelia Sm., Astroloma R.Br., Croninia J.M. Powell and Coleanthera Stschegl. Parsimony and Bayesian analyses of sequence data from four plastid markers (rbcL, matK, trnH–psbA, and atpB–rbcL), and the nuclear ribosomal internal transcribed spacer (ITS) for 207 taxa corroborate the polyphyly of the genera Astroloma, Leucopogon and Styphelia and resolve 12 well supported groups. Of these groups, two can be distinguished by unique morphological features and another six by different character combinations. The remaining groups are morphologically heterogeneous and inconsistent, and not readily distinguishable. A number of species remain ungrouped either because their phylogenetic relationships are not clear or because they do not show strong morphological affinities with the group to which they have a close phylogenetic relationship. Translating the results into a phylogenetic classification is a choice between accepting a single, large genus or at least 12 smaller genera. The first option would result in a heterogeneous assemblage conveying limited morphological information. The multi-generic option would be a better reflection of the morphological ersity of the clade, but would result in many genera lacking readily observable, diagnostic morphological characters. We prioritise the nomenclatural stability inherent in the former approach and advocate expanding Styphelia to include all taxa in the Styphelia–Astroloma clade.
Publisher: Elsevier BV
Date: 05-2018
No related grants have been discovered for Caroline Puente-Lelievre.