ORCID Profile
0009-0000-7128-8097
Current Organisation
University of Technology Sydney Faculty of Engineering and Information Technology
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Publisher: Elsevier BV
Date: 11-2022
DOI: 10.1016/J.CTRV.2022.102439
Abstract: Clinically, HPV-positive oropharyngeal cancers (OPCs) have been shown to have a distinct prognosis, compared to HPV-negative tumours, particularly in survival rates and responses to treatment. These patients have better survival chances and improved prognosis, indicating that a more exhaustive knowledge of these distinctions would aid in the discovery of clinical approaches for both HPV-positive and negative tumours. Furthermore, there is increasing evidence that HPV-related oropharyngeal cancers constitute an epidemiological, molecular, and clinical distinct form as compared to non-HPV related ones therefore, the treatment of these specific subtype of oropharyngeal cancers should adopt a distinct clinical treatment pipeline. Our review will examine the current approaches for the diagnosis and treatment of OPC and discuss the relevance of de-escalation clinical trials in progress.
Publisher: Elsevier BV
Date: 08-2018
DOI: 10.1016/J.VIROL.2018.05.022
Abstract: Human papillomavirus (HPV), notably type 16, is a risk factor for up to 75% of oropharyngeal squamous cell carcinomas (SCC). It has been demonstrated that small non-coding RNAs known as microRNAs play a vital role in the cellular transformation process. In this study, we used an LNA array to further investigate the impact of HPV16 on the expression of microRNAs in oropharyngeal (tonsillar) cancer. A number of miRNAs were found to be deregulated, with miR-496 showing a four-fold decrease. Over-expression of the high risk E6 oncoprotein down-regulated miR-496, impacting upon the post-transcriptional control of the transcription factor E2F2. These HPV specific miRNAs were integrated with the HPV16 interactome to identify possible mechanistic pathways. These analyses provide insights into novel molecular interactions between HPV16 and miRNAs in oropharyngeal cancers.
Publisher: Wiley
Date: 20-07-2020
DOI: 10.1002/HED.26348
Publisher: Springer Science and Business Media LLC
Date: 10-08-2020
DOI: 10.1186/S12936-020-03360-Z
Abstract: Extracellular vesicles (EVs) have been broadly studied in malaria for nearly a decade. These vesicles carry various functional biomolecules including RNA families such as microRNAs (miRNA). These EVs-derived microRNAs have numerous roles in host-parasite interactions and are considered promising biomarkers for disease severity. However, this field lacks clinical studies of malaria-infected s les. In this study, EV specific miRNAs were isolated from the plasma of patients from Thailand infected with Plasmodium vivax and Plasmodium falciparum . In addition, it is postulated that these miRNAs were differentially expressed in these groups of patients and had a role in disease onset through the regulation of specific target genes. EVs were purified from the plasma of Thai P. vivax -infected patients (n = 19), P. falciparum -infected patients (n = 18) and uninfected in iduals (n = 20). EV-derived miRNAs were then prepared and abundance of hsa-miR-15b-5p, hsa-miR-16-5p, hsa-let-7a-5p and hsa-miR-150-5p was assessed in these s les. Quantitative polymerase chain reaction was performed, and relative expression of each miRNA was calculated using hsa-miR-451a as endogenous control. Then, the targets of up-regulated miRNAs and relevant pathways were predicted by using bioinformatics. Receiver Operating Characteristic with Area under the Curve (AUC) was then calculated to assess their diagnostic potential. The relative expression of hsa-miR-150-5p and hsa-miR-15b-5p was higher in P. vivax -infected patients compared to uninfected in iduals, but hsa-let-7a-5p was up-regulated in both P. vivax -infected patients and P. falciparum -infected patients. Bioinformatic analysis revealed that these miRNAs might regulate genes involved in the malaria pathway including the adherens junction and the transforming growth factor-β pathways. All up-regulated miRNAs could potentially be used as disease biomarkers as determined by AUC however, the sensitivity and specificity require further investigation. An upregulation of hsa-miR-150-5p and hsa-miR-15b-5p was observed in P. vivax -infected patients while hsa-let-7a-5p was up-regulated in both P. vivax -infected and P. falciparum -infected patients. These findings will require further validation in larger cohort groups of malaria patients to fully understand the contribution of these EVs miRNAs to malaria detection and biology.
Location: Australia
No related grants have been discovered for Fiona Deutsch.