ORCID Profile
0000-0003-0358-2774
Current Organisations
SLAC National Accelerator Laboratory
,
NASA Goddard Space Flight Center
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Publisher: American Astronomical Society
Date: 18-08-2023
Abstract: We present an analysis of microlensing event OGLE-2019-BLG-0825. This event was identified as a planetary candidate by preliminary modeling. We find that significant residuals from the best-fit static binary-lens model exist and a xallarap effect can fit the residuals very well and significantly improves χ 2 values. On the other hand, by including the xallarap effect in our models, we find that binary-lens parameters such as mass ratio, q , and separation, s , cannot be constrained well. However, we also find that the parameters for the source system such as the orbital period and semimajor axis are consistent between all the models we analyzed. We therefore constrain the properties of the source system better than the properties of the lens system. The source system comprises a G-type main-sequence star orbited by a brown dwarf with a period of P ∼ 5 days. This analysis is the first to demonstrate that the xallarap effect does affect binary-lens parameters in planetary events. It would not be common for the presence or absence of the xallarap effect to affect lens parameters in events with long orbital periods of the source system or events with transits to caustics, but in other cases, such as this event, the xallarap effect can affect binary-lens parameters.
Publisher: IOP Publishing
Date: 13-08-2013
Publisher: The Optical Society
Date: 20-01-2015
DOI: 10.1364/OE.23.001213
Publisher: SPIE
Date: 19-10-2012
DOI: 10.1117/12.930075
Publisher: American Physical Society (APS)
Date: 28-02-2014
Publisher: International Union of Crystallography (IUCr)
Date: 15-03-2012
DOI: 10.1107/S0021889812002312
Abstract: In order to address the specific needs of the emerging technique of `serial femtosecond crystallography', in which structural information is obtained from small crystals illuminated by an X-ray free-electron laser, a new software suite has been created. The constituent programs deal with viewing, indexing, integrating, merging and evaluating the quality of the data, and also simulating patterns. The specific challenges addressed chiefly concern the indexing and integration of large numbers of diffraction patterns in an automated manner, and so the software is designed to be fast and to make use of multi-core hardware. Other constituent programs deal with the merging and scaling of large numbers of intensities from randomly oriented snapshot diffraction patterns. The suite uses a generalized representation of a detector to ease the use of more complicated geometries than those familiar in conventional crystallography. The suite is written in C with supporting Perl and shell scripts, and is available as source code under version 3 or later of the GNU General Public License.
Publisher: International Union of Crystallography (IUCr)
Date: 21-03-2022
DOI: 10.1107/S1600577522001862
Abstract: Serial crystallography of membrane proteins often employs high-viscosity injectors (HVIs) to deliver micrometre-sized crystals to the X-ray beam. Typically, the carrier medium is a lipidic cubic phase (LCP) media, which can also be used to nucleate and grow the crystals. However, despite the fact that the LCP is widely used with HVIs, the potential impact of the injection process on the LCP structure has not been reported and hence is not yet well understood. The self-assembled structure of the LCP can be affected by pressure, dehydration and temperature changes, all of which occur during continuous flow injection. These changes to the LCP structure may in turn impact the results of X-ray diffraction measurements from membrane protein crystals. To investigate the influence of HVIs on the structure of the LCP we conducted a study of the phase changes in monoolein/water and monoolein/buffer mixtures during continuous flow injection, at both atmospheric pressure and under vacuum. The reservoir pressure in the HVI was tracked to determine if there is any correlation with the phase behaviour of the LCP. The results indicated that, even though the reservoir pressure underwent (at times) significant variation, this did not appear to correlate with observed phase changes in the s le stream or correspond to shifts in the LCP lattice parameter. During vacuum injection, there was a three-way coexistence of the gyroid cubic phase, diamond cubic phase and lamellar phase. During injection at atmospheric pressure, the coexistence of a cubic phase and lamellar phase in the monoolein/water mixtures was also observed. The degree to which the lamellar phase is formed was found to be strongly dependent on the co-flowing gas conditions used to stabilize the LCP stream. A combination of laboratory-based optical polarization microscopy and simulation studies was used to investigate these observations.
Publisher: Springer Science and Business Media LLC
Date: 11-12-2012
DOI: 10.1038/NCOMMS2288
Abstract: Diffractive imaging with free-electron lasers allows structure determination from ensembles of weakly scattering identical nanoparticles. The ultra-short, ultra-bright X-ray pulses provide snapshots of the randomly oriented particles frozen in time, and terminate before the onset of structural damage. As signal strength diminishes for small particles, the synthesis of a three-dimensional diffraction volume requires simultaneous involvement of all data. Here we report the first application of a three-dimensional spatial frequency correlation analysis to carry out this synthesis from noisy single-particle femtosecond X-ray diffraction patterns of nearly identical s les in random and unknown orientations, collected at the Linac Coherent Light Source. Our demonstration uses unsupported test particles created via aerosol self-assembly, and composed of two polystyrene spheres of equal diameter. The correlation analysis avoids the need for orientation determination entirely. This method may be applied to the structural determination of biological macromolecules in solution.
Publisher: The Optical Society
Date: 12-12-2019
DOI: 10.1364/OE.27.037816
Publisher: Springer Science and Business Media LLC
Date: 10-05-2021
DOI: 10.1038/S41467-021-22590-6
Abstract: MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production. We previously observed that the TIR domain of MAL (MAL TIR ) forms filaments in vitro and induces formation of crystalline higher-order assemblies of the MyD88 TIR domain (MyD88 TIR ). These crystals are too small for conventional X-ray crystallography, but are ideally suited to structure determination by microcrystal electron diffraction (MicroED) and serial femtosecond crystallography (SFX). Here, we present MicroED and SFX structures of the MyD88 TIR assembly, which reveal a two-stranded higher-order assembly arrangement of TIR domains analogous to that seen previously for MAL TIR . We demonstrate via mutagenesis that the MyD88 TIR assembly interfaces are critical for TLR4 signaling in vivo, and we show that MAL promotes unidirectional assembly of MyD88 TIR . Collectively, our studies provide structural and mechanistic insight into TLR signal transduction and allow a direct comparison of the MicroED and SFX techniques.
Publisher: AIP Publishing
Date: 15-12-2016
DOI: 10.1063/1.4972069
Abstract: Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. It provides (i) room temperature structures at near atomic resolution, (ii) time resolution ranging from microseconds to seconds, and (iii) convenient reaction initiation. It outruns radiation damage by using femtosecond X-ray pulses allowing damage and chemistry to be separated. Here, we demonstrate that MISC is feasible at an X-ray free electron laser by studying the reaction of M. tuberculosis ß-lactamase microcrystals with ceftriaxone antibiotic solution. Electron density maps of the apo-ß-lactamase and of the ceftriaxone bound form were obtained at 2.8 Å and 2.4 Å resolution, respectively. These results pave the way to study cyclic and non-cyclic reactions and represent a new field of time-resolved structural dynamics for numerous substrate-triggered biological reactions.
Publisher: Springer Science and Business Media LLC
Date: 09-07-2014
DOI: 10.1038/NATURE13453
Publisher: SPIE
Date: 17-10-2012
DOI: 10.1117/12.929294
Publisher: The Optical Society
Date: 14-11-2013
DOI: 10.1364/OE.21.028729
Publisher: Springer Science and Business Media LLC
Date: 02-2011
DOI: 10.1038/NATURE09748
Publisher: American Astronomical Society
Date: 07-2022
Abstract: We report the first unambiguous detection and mass measurement of an isolated stellar-mass black hole (BH). We used the Hubble Space Telescope (HST) to carry out precise astrometry of the source star of the long-duration ( t E ≃ 270 days), high-magnification microlensing event MOA-2011-BLG-191/OGLE-2011-BLG-0462 (hereafter designated as MOA-11-191/OGLE-11-462), in the direction of the Galactic bulge. HST imaging, conducted at eight epochs over an interval of 6 yr, reveals a clear relativistic astrometric deflection of the background star’s apparent position. Ground-based photometry of MOA-11-191/OGLE-11-462 shows a parallactic signature of the effect of Earth’s motion on the microlensing light curve. Combining the HST astrometry with the ground-based light curve and the derived parallax, we obtain a lens mass of 7.1 ± 1.3 M ⊙ and a distance of 1.58 ± 0.18 kpc. We show that the lens emits no detectable light, which, along with having a mass higher than is possible for a white dwarf or neutron star, confirms its BH nature. Our analysis also provides an absolute proper motion for the BH. The proper motion is offset from the mean motion of Galactic disk stars at similar distances by an amount corresponding to a transverse space velocity of ∼45 km s −1 , suggesting that the BH received a “natal kick” from its supernova explosion. Previous mass determinations for stellar-mass BHs have come from radial velocity measurements of Galactic X-ray binaries and from gravitational radiation emitted by merging BHs in binary systems in external galaxies. Our mass measurement is the first for an isolated stellar-mass BH using any technique.
Publisher: The Optical Society
Date: 29-01-2014
DOI: 10.1364/OE.22.002497
Publisher: American Astronomical Society
Date: 31-08-2022
Abstract: We report the light-curve analysis for the event MOA-2020-BLG-135, which leads to the discovery of a new Neptune-class planet, MOA-2020-BLG-135Lb. With a derived mass ratio of q = 1.52 − 0.31 + 0.39 × 10 − 4 and separation s ≈ 1, the planet lies exactly at the break and likely peak of the exoplanet mass-ratio function derived by the Microlensing Observations in Astrophysics (MOA) Collaboration. We estimate the properties of the lens system based on a Galactic model and considering two different Bayesian priors: one assuming that all stars have an equal planet-hosting probability and the other that planets are more likely to orbit more-massive stars. With a uniform host mass prior, we predict that the lens system is likely to be a planet of mass m planet = 11.3 − 6.9 + 19.2 M ⊕ and a host star of mass M host = 0.23 − 0.14 + 0.39 M ⊙ , located at a distance D L = 7.9 − 1.0 + 1.0 kpc . With a prior that holds that planet occurrence scales in proportion to the host-star mass, the estimated lens system properties are m planet = 25 − 15 + 22 M ⊕ , M host = 0.53 − 0.32 + 0.42 M ⊙ , and D L = 8.3 − 1.0 + 0.9 kpc . This planet qualifies for inclusion in the extended MOA-II exoplanet microlens s le.
Publisher: International Union of Crystallography (IUCr)
Date: 20-10-2017
DOI: 10.1107/S2052252517014324
Abstract: Serial diffraction data collected at the Linac Coherent Light Source from crystalline amyloid fibrils delivered in a liquid jet show that the fibrils are well oriented in the jet. At low fibril concentrations, diffraction patterns are recorded from single fibrils these patterns are weak and contain only a few reflections. Methods are developed for determining the orientation of patterns in reciprocal space and merging them in three dimensions. This allows the in idual structure litudes to be calculated, thus overcoming the limitations of orientation and cylindrical averaging in conventional fibre diffraction analysis. The advantages of this technique should allow structural studies of fibrous systems in biology that are inaccessible using existing techniques.
Publisher: Springer Science and Business Media LLC
Date: 19-11-2020
DOI: 10.1038/S41597-020-00745-2
Abstract: Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μ m x 1.7 μ m full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.
Publisher: Springer Science and Business Media LLC
Date: 08-2016
Abstract: Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological s les without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The s le was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.
Publisher: AIP Publishing
Date: 21-04-2015
DOI: 10.1063/1.4918726
Abstract: Intense femtosecond x-ray pulses from free-electron laser sources allow the imaging of in idual particles in a single shot. Early experiments at the Linac Coherent Light Source (LCLS) have led to rapid progress in the field and, so far, coherent diffractive images have been recorded from biological specimens, aerosols, and quantum systems with a few-tens-of-nanometers resolution. In March 2014, LCLS held a workshop to discuss the scientific and technical challenges for reaching the ultimate goal of atomic resolution with single-shot coherent diffractive imaging. This paper summarizes the workshop findings and presents the roadmap toward reaching atomic resolution, 3D imaging at free-electron laser sources.
Publisher: The Optical Society
Date: 13-05-2013
DOI: 10.1364/OE.21.012385
Publisher: Springer Science and Business Media LLC
Date: 14-04-2020
DOI: 10.1038/S41467-020-15610-4
Abstract: X-ray free-electron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed upon synchrotron measurements by radiation damage. The need for very short XFEL pulses is relieved through gating of Bragg diffraction by loss of crystalline order as damage progresses, but not if ionization events are spatially non-uniform due to underlying elemental distributions, as in biological s les. Indeed, correlated movements of iron and sulfur ions were observed in XFEL-irradiated ferredoxin microcrystals using unusually long pulses of 80 fs. Here, we report a femtosecond time-resolved X-ray pump/X-ray probe experiment on protein nanocrystals. We observe changes in the protein backbone and aromatic residues as well as disulfide bridges. Simulations show that the latter’s correlated structural dynamics are much slower than expected for the predicted high atomic charge states due to significant impact of ion caging and plasma electron screening. This indicates that dense-environment effects can strongly affect local radiation damage-induced structural dynamics.
Publisher: American Astronomical Society
Date: 24-03-2023
Abstract: We analyze the MOA-2020-BLG-208 gravitational microlensing event and present the discovery and characterization of a new planet, MOA-2020-BLG-208Lb, with an estimated sub-Saturn mass. With a mass ratio q = 3.17 − 0.26 + 0.28 × 10 − 4 , the planet lies near the peak of the mass-ratio function derived by the MOA collaboration and near the edge of expected s le sensitivity. For these estimates we provide results using two mass-law priors: one assuming that all stars have an equal planet-hosting probability, and the other assuming that planets are more likely to orbit around more massive stars. In the first scenario, we estimate that the lens system is likely to be a planet of mass m planet = 46 − 24 + 42 M ⊕ and a host star of mass M host = 0.43 − 0.23 + 0.39 M ⊙ , located at a distance D L = 7.49 − 1.13 + 0.99 kpc . For the second scenario, we estimate m planet = 69 − 34 + 37 M ⊕ , M host = 0.66 − 0.32 + 0.35 M ⊙ , and D L = 7.81 − 0.93 + 0.93 kpc . The planet has a projected separation as a fraction of the Einstein ring radius s = 1.3807 − 0.0018 + 0.0018 . As a cool sub-Saturn-mass planet, this planet adds to a growing collection of evidence for revised planetary formation models.
Publisher: International Union of Crystallography (IUCr)
Date: 16-02-2011
Location: United States of America
Location: United States of America
No related grants have been discovered for Andrew Aquila.