ORCID Profile
0000-0001-8289-0497
Current Organisation
SLAC National Accelerator Laboratory
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Publisher: American Association for the Advancement of Science (AAAS)
Date: 06-05-2016
Abstract: Many biological processes depend on detecting and responding to light. The response is often mediated by a structural change in a protein that begins when absorption of a photon causes isomerization of a chromophore bound to the protein. Pande et al. used x-ray pulses emitted by a free electron laser source to conduct time-resolved serial femtosecond crystallography in the time range of 100 fs to 3 ms. This allowed for the real-time tracking of the trans-cis isomerization of the chromophore in photoactive yellow protein and the associated structural changes in the protein. Science , this issue p. 725
Publisher: Springer Science and Business Media LLC
Date: 19-10-2016
DOI: 10.1038/SREP35279
Abstract: Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 Å resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
Publisher: American Association for the Advancement of Science (AAAS)
Date: 25-03-2011
Abstract: Three techniques are used to probe the pseudogap state of cuprate high-temperature superconductors.
Location: United States of America
Location: United States of America
Location: United States of America
No related grants have been discovered for Jake Koralek.