ORCID Profile
0000-0002-1383-5256
Current Organisations
Hong Kong University of Science and Technology
,
CQM - Centro de Química da Madeira, Universidade da Madeira
,
Université Paris Descartes
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Related Links
Publications
Mechanism of Cationic Phosphorus Dendrimer Toxicity against Murine Neural Cell Lines
Publisher: American Chemical Society (ACS)
Date: 30-07-2013
DOI: 10.1021/MP4003255
Abstract: The purpose of this manuscript is to study the toxic responses against murine embryonic hippoc al cells (mHippoE-18) and neuroblastoma cells (N2a) to treatment with cationic phosphorus dendrimers (CPD). Two low generations of CPD--generation 2 (G2) and generation 3 (G3)--were applied to cell cultures to monitor events leading to either apoptosis or necrosis. These processes were analyzed using several bioassays, which included the detection of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm) alterations, morphology changes, apoptotic and dead cells, cytochrome c (Cyt c) release, caspase 3 activity, DNA fragmentation, as well as changes in cell cycle phases distribution. The results showed that CPD became highly cytotoxic at concentrations above 1 μM and at 0.7 μM in the case of G3 for mHippoE-18 cells. The toxicity was manifested by a pronounced decrease in cell viability, which is correlated with disturbances in cellular activities, such as massive ROS generation. The breakdown of cellular processes leads mainly to the necrotic cell death. Our findings are of high importance in the context of further biomedical studies on CPD.
Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (B). Efficiency of pharmacological action
Publisher: Elsevier BV
Date: 05-2015
DOI: 10.1016/J.IJPHARM.2015.03.034
Abstract: This paper examines a perspective to use newly engineered nanomaterials as effective and safe carriers for gene therapy of cancer. Three different groups of cationic dendrimers (PAMAM, phosphorus, and carbosilane) were complexed with anticancer siRNA and the biophysical properties of the dendriplexes created were analyzed. The potential of the dendrimers as nanocarriers for anticancer Bcl-xl, Bcl-2, Mcl-1 siRNAs and additionally a scrambled sequence siRNA has been explored. Dendrimer/siRNA complexes were characterised by various methods including fluorescence, zeta potential, dynamic light scattering, circular dichroism, gel electrophoresis and transmission electron microscopy. In this part of study, the transfection of complexes in HeLa and HL-60 cells was analyzed using both single apoptotic siRNAs and a mixture (cocktail) of them. Cocktails were more effective than single siRNAs, allowing one to decrease siRNAs concentration in treating cells. The dendrimers were compared as siRNA carriers, the most effective being the phosphorus-based ones. However, they were also the most cytotoxic on their own, so that in this regard the application of all dendrimers in anticancer therapy will be discussed.
Visualization and monitoring of dynamic damaging–healing processes of polymers by using AIEgen-loaded multifunctional microcapsules
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2TA02918A
Abstract: The damaging–healing processes of multifunctional polymer coatings with self-healing capability, photoprotection properties, and water-proof functions are clearly visualized and monitored using AIEgen-loaded multifunctional microcapsules.
A Facile Strategy To Prepare Smart Coatings with Autonomous Self-Healing and Self-Reporting Functions
Publisher: American Chemical Society (ACS)
Date: 30-12-2019
Abstract: Herein, we report a smart coating with autonomous self-healing and self-reporting functions by simple integration of one-component microcapsules into the matrix without external intervention. The microcapsules containing hexamethylene diisocyanate (HDI) solution of aggregation-induced emission luminogens (AIEgens) were synthesized, and their properties, such as their composition, thermal stability, morphology, and damage-indicating ability, were investigated systematically. The AIEgen/HDI microcapsule-embedded coatings display adaptive self-repair of scratches and simultaneous high-contrast indication of the healed damage. Two commercialized AIEgens, tetraphenylethylene (TPE) and its derivative with dimethoxyl and benzylidene-methyloxazolone moieties (DM-TPE-BMO), were utilized as ex les to demonstrate the feasibility of this concept in erse polymer matrixes (including blue autofluorescent matrixes). It was found that the content of AIEgens can even be lowered to 0.05 wt %. This facile, economical, and feasible strategy toward the dual functions of self-repairing and self-sensing provides a new route for enhancing the longevity and reliability of polymer coatings, which is appealing and of great importance in practical applications.
Construction of polydopamine-coated gold nanostars for CT imaging and enhanced photothermal therapy of tumors: an innovative theranostic strategy
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6TB00773B
Abstract: A theranostic nanoplatform for in vivo CT imaging and enhanced PTT of tumors is reported.
Promising Low-Toxicity of Viologen-Phosphorus Dendrimers against Embryonic Mouse Hippocampal Cells
Publisher: MDPI AG
Date: 30-09-2013
A promising dual mode SPECT/CT imaging platform based on99mTc-labeled multifunctional dendrimer-entrapped gold nanoparticles
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7TB00543A
Abstract: Multifunctional 99m Tc-labeled dendrimer-entrapped gold nanoparticles modified with different surface groups can be used for preferential SPECT/CT imaging of different organs.
Related Organisations
CQM - Centro De Química Da Madeira, Universidade Da Madeira
Location: Portugal
Related Funding Activities
No related grants have been discovered for Serge Mignani.