ORCID Profile
0000-0002-2625-9876
Current Organisation
YTEK Pty Ltd
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Publisher: Elsevier BV
Date: 02-2016
Publisher: Elsevier BV
Date: 04-2007
DOI: 10.1016/J.NEUROSCIENCE.2007.01.012
Abstract: We have recently demonstrated that co-administration of 3,4-methylenedioxymeth hetamine (MDMA, "ecstasy") with the reversible monoamine oxidase type A (MAO-A) inhibitor moclobemide at an ambient temperature of 22 degrees C significantly increases striatal 5-HT outflow and 5-HT-mediated behaviors. In the present study, using microdialysis, we examined the effects of co-administration of MDMA or para-methoxy hetamine (PMA) with moclobemide on striatal 5-HT outflow at the elevated ambient temperatures of 30 degrees C. S les were collected every 30 min for 4 h and analyzed by high-performance liquid chromatography assay with electrochemical detection (HPLC-ED). 5-HT-mediated effects on body temperature and behavior were also recorded. Rats were treated with either saline or 20 mg/kg (i.p.) moclobemide, followed by 10 mg/kg (i.p.) MDMA, 10 mg/kg (i.p.) PMA or saline 60 min later. Both MDMA and PMA produced significant increases in 5-HT outflow (370% peak and 309% peak, respectively, P<0.05). MDMA and PMA significantly increased body temperature (+2.0 degrees C and +2.1 degrees C, respectively, P<0.01) and drug-related behaviors (P<0.05). When MDMA or PMA was co-administered with moclobemide, additional significant increases were seen in 5-HT outflow (850% peak, P<0.01 and 1450% peak, P<0.001, respectively) and only MDMA showed additional significant increase in body temperature (+5.0 degrees C, P<0.001). No additional increases were seen in behavioral activity. When moclobemide was co-administered with MDMA, sustained increases in body temperature were recorded that were significantly higher than with MDMA alone and such increases were not observed in our previous study at normal room temperature. Our results suggest greater risk of MDMA-induced adverse effects on body temperature regulation, compared with PMA, when used in combination with moclobemide at elevated ambient temperatures.
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7SC01647F
Abstract: A prodrug strategy for the release of the gasotransmitter carbon monoxide (CO) at physiological pH, based upon 3 a -bromo-norborn-2-en-7-one Diels–Alder cycloadducts has been developed.
Publisher: Elsevier BV
Date: 12-2020
Publisher: American Society for Pharmacology & Experimental Therapeutics (ASPET)
Date: 02-04-2013
Abstract: Ketamine is primarily metabolized to norketamine by hepatic CYP2B6 and CYP3A4-mediated N-demethylation. However, the relative contribution from each enzyme remains controversial. The CYP2B6*6 allele is associated with reduced enzyme expression and activity that may lead to interin idual variability in ketamine metabolism. We examined the N-demethylation of in idual ketamine enantiomers using human liver microsomes (HLMs) genotyped for the CYP2B6*6 allele, insect cell-expressed recombinant CYP2B6 and CYP3A4 enzymes, and COS-1 cell-expressed recombinant CYP2B6.1 and CYP2B6.6 protein variant. Effects of CYP-selective inhibitors on norketamine formation were also determined in HLMs. The two-enzyme Michaelis-Menten model best fitted the HLM kinetic data. The Michaelis-Menten constants (K(m)) for the high-affinity enzyme and the low-affinity enzyme were similar to those for the expressed CYP2B6 and CYP3A4, respectively. The intrinsic clearance for both ketamine enantiomers by the high-affinity enzyme in HLMs with CYP2B6*1/*1 genotype were at least 2-fold and 6-fold higher, respectively, than those for CYP2B6*1/*6 genotype and CYP2B6*6/*6 genotype. The V(max) and K(m) values for CYP2B6.1 were approximately 160 and 70% of those for CYP2B6.6, respectively. N,N'N'-triethylenethiophosphoramide (thioTEPA) (CYP2B6 inhibitor, 25 μM) and the monoclonal antibody against CYP2B6 but not troleandomycin (CYP3A4 inhibitor, 25 μM) or the monoclonal antibody against CYP3A4 inhibited ketamine N-demethylation at clinically relevant concentrations. The degree of inhibition was significantly reduced in HLMs with the CYP2B6*6 allele (gene-dose P < 0.05). These results indicate a major role of CYP2B6 in ketamine N-demethylation in vitro and a significant impact of the CYP2B6*6 allele on enzyme-ketamine binding and catalytic activity.
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1NR00150G
Abstract: Printed electronic sensors offer a breakthrough in the availability of low-cost devices for improving the quality of human life. Conductive ink is the core of printing technology and is one of the fastest growing ink industries.
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0NR04933F
Abstract: Graphene and related 2D materials offer an ideal platform for next generation disruptive technologies and in particular the potential to produce printed electronic devices with low cost and high throughput.
Publisher: Wiley
Date: 15-11-2021
Abstract: Wearable sensors are currently one of the top emerging areas with enormous growth potential. Low‐cost fabrication techniques using simple and scalable printing technologies are making a significant impact on their development. Recent advances in high‐performance gas/vapor sensors based on carbon nanomaterials have shown potential applications ranging from disease diagnostics to environmental monitoring and defences. Herein, a hybrid sensing material of 1D carbon nanotubes (CNTs) and 2D graphene is developed, and a conductive ink is formulated, which is applied for fabricating a nitrogen dioxide (NO 2 ) gas sensor array within a compact design utilizing extrusion printing. To improve NO 2 ‐sensing performance and optimal operating temperature, a reverse‐side layer is designed, which combines MXene and poly(3,4‐ethylenedioxythiophene)‐doped poly(styrene sulfonate) (PEDOT:PSS), and functions as a Joule heater. The printed CNT–graphene‐based sensor with an embedded MXene/PEDOT:PSS heater is capable of detecting trace amounts of NO 2 gas (1 ppm) at 65 °C. The sensor is able to distinguish between various gases/volatile organic compounds and target NO 2 gas based on their chemical affinities. The printed CNT–graphene sensor array also demonstrates a high‐level of recoverability, satisfied stability, durability, and reproducibility, which render this sensor a suitable candidate for practical applications.
No related grants have been discovered for Nathan Stanley.