ORCID Profile
0000-0002-8512-2802
Current Organisation
University of Sheffield
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Publisher: Wiley
Date: 29-04-2009
DOI: 10.1111/J.1469-8137.2009.02851.X
Abstract: Pseudomonas fluorescens WCS417r bacteria and beta-aminobutyric acid can induce disease resistance in Arabidopsis, which is based on priming of defence. In this study, we examined the differences and similarities of WCS417r- and beta-aminobutyric acid-induced priming. Both WCS417r and beta-aminobutyric acid prime for enhanced deposition of callose-rich papillae after infection by the oomycete Hyaloperonospora arabidopsis. This priming is regulated by convergent pathways, which depend on phosphoinositide- and ABA-dependent signalling components. Conversely, induced resistance by WCS417r and beta-aminobutyric acid against the bacterial pathogen Pseudomonas syringae are controlled by distinct NPR1-dependent signalling pathways. As WCS417r and beta-aminobutyric acid prime jasmonate- and salicylate-inducible genes, respectively, we subsequently investigated the role of transcription factors. A quantitative PCR-based genome-wide screen for putative WCS417r- and beta-aminobutyric acid-responsive transcription factor genes revealed distinct sets of priming-responsive genes. Transcriptional analysis of a selection of these genes showed that they can serve as specific markers for priming. Promoter analysis of WRKY genes identified a putative cis-element that is strongly over-represented in promoters of 21 NPR1-dependent, beta-aminobutyric acid-inducible WRKY genes. Our study shows that priming of defence is regulated by different pathways, depending on the inducing agent and the challenging pathogen. Furthermore, we demonstrated that priming is associated with the enhanced expression of transcription factors.
Publisher: eLife Sciences Publications, Ltd
Date: 04-01-2019
DOI: 10.7554/ELIFE.40655
Abstract: Variation in DNA methylation enables plants to inherit traits independently of changes to DNA sequence. Here, we have screened an Arabidopsis population of epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These lines share the same genetic background, but show variation in heritable patterns of DNA methylation. We identified four epigenetic quantitative trait loci (epiQTLs) that provide quantitative resistance without reducing plant growth or resistance to other (a)biotic stresses. Phenotypic characterisation and RNA-sequencing analysis revealed that Hpa-resistant epiRILs are primed to activate defence responses at the relatively early stages of infection. Collectively, our results show that hypomethylation at selected pericentromeric regions is sufficient to provide quantitative disease resistance, which is associated with genome-wide priming of defence-related genes. Based on comparisons of global gene expression and DNA methylation between the wild-type and resistant epiRILs, we discuss mechanisms by which the pericentromeric epiQTLs could regulate the defence-related transcriptome.
Publisher: Springer Science and Business Media LLC
Date: 03-10-2018
DOI: 10.1038/S41598-018-32448-5
Abstract: Progeny of heavily diseased plants develop transgenerational acquired resistance (TAR). In Arabidopsis, TAR can be transmitted over one stress-free generation. Although DNA methylation has been implicated in the regulation of TAR, the relationship between TAR and global DNA methylation remains unknown. Here, we characterised the methylome of TAR-expressing Arabidopsis at different generations after disease exposure. Global clustering of cytosine methylation revealed TAR-related patterns in the F3 generation, but not in the F1 generation. The majority of differentially methylated positions (DMPs) occurred at CG context in gene bodies. TAR in F3 progeny after one initial generation of disease, followed by two stress-free generations, was lower than TAR in F3 progeny after three successive generations of disease. This difference in TAR effectiveness was proportional to the intensity of differential methylation at a sub-set of cytosine positions. Comparison of TAR-related DMPs with previously characterised cytosine methylation in mutation accumulation lines revealed that ancestral disease stress preferentially acts on methylation-labile cytosine positions, but also extends to methylation-stable positions. Thus, the TAR-related impact of ancestral disease extends beyond stochastic variation in DNA methylation. Our study has shown that the Arabidopsis epigenome responds globally to disease in previous generations and we discuss its contribution to TAR.
Publisher: Elsevier BV
Date: 11-2008
Publisher: Springer Science and Business Media LLC
Date: 19-02-2018
DOI: 10.1038/S41477-018-0108-Y
Abstract: The magnitude of future climate change could be moderated by immediately reducing the amount of CO
Publisher: Cold Spring Harbor Laboratory
Date: 21-08-2023
DOI: 10.1101/2023.08.21.554086
Abstract: The phytohormone salicylic acid (SA) is an important molecular signal that mediates pathogen defence mechanisms, including triggering Arabidopsis immune responses to the hemi-biotroph Pseudomonas syringae pv. tomato ( Pst ). SA induces the expression of a myriad of defence genes via its receptor and transcriptional regulator NONEXPRESSER OF PR GENES 1 (NPR1). Here, we used chlorophyll fluorescence imaging of F v /F m , to detect damage to photosystem II before Pst -induced disease symptoms were visible. We observed that the pathogen only induced damage, and subsequent cell death, in mature leaves while developing leaves in the center of the rosette appeared to be protected. However, in the npr1-1 mutant, Pst -infected mature leaves were able to systemically transmit a signal that caused damage to the photosynthetic machinery in uninfected young leaves. Reductions in F v /F m could also be induced systemically in developing npr1-1 leaves by high levels of SA in mature leaves, and rescued by SA biosynthesis deficiency in npr1-1sid2-2 mutants. Together, these results indicate that, in addition to its well-known role as a positive regulator of SA responses, NPR1 also acts to suppress SA-dependent immune responses and thereby protects developing leaves from autoimmune damage.
Publisher: Wiley
Date: 10-06-2019
DOI: 10.1111/PCE.13570
Publisher: American Association for the Advancement of Science (AAAS)
Date: 12-11-2021
Abstract: Centromeres attach chromosomes to spindle microtubules during cell ision and, despite this conserved role, show paradoxically rapid evolution and are typified by complex repeats. We used long-read sequencing to generate the Col-CEN
Publisher: Springer Science and Business Media LLC
Date: 28-04-2014
Publisher: Wiley
Date: 07-09-2016
DOI: 10.1111/TPJ.13252
Publisher: American Association for the Advancement of Science (AAAS)
Date: 29-09-2023
Publisher: eLife Sciences Publications, Ltd
Date: 21-11-2018
Publisher: Scientific Societies
Date: 10-2019
Publisher: Cold Spring Harbor Laboratory
Date: 16-03-2023
DOI: 10.1101/2023.03.15.532766
Abstract: Molecular clocks are the basis for dating the ergence between lineages over macro-evolutionary timescales (~10 5 -10 8 years). However, classical DNA-based clocks tick too slowly to inform us about the recent past. Here, we demonstrate that stochastic DNA methylation changes at a subset of cytosines in plant genomes possess a clock-like behavior. This ‘epimutation-clock’ is orders of magnitude faster than DNA-based clocks and enables phylogenetic explorations on a scale of years to centuries. We show experimentally that epimutation-clocks recapitulate known topologies and branching times of intra-species phylogenetic trees in the selfing plant A. thaliana and the clonal seagrass Z. marina , which represent two major modes of plant reproduction. This discovery will open new possibilities for high-resolution temporal studies of plant bio ersity.
Publisher: Wiley
Date: 21-06-2010
Publisher: Springer Science and Business Media LLC
Date: 25-05-2018
DOI: 10.1038/S41477-018-0162-5
Abstract: In the version of this Perspective originally published, 'acidification' was incorrectly spelt as 'adification' in Fig. 4. This has now been corrected.
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Jurriaan Ton.