ORCID Profile
0000-0001-9033-0158
Current Organisations
Hong Kong University of Science and Technology
,
City University of Hong Kong
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Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1EN00021G
Abstract: AgNP toxicity was attributed to dissolved Ag + , which was released, transported, and concentrated in the mitochondria, finally leading to exhaustion of the reserve respiratory capacity and cell death.
Publisher: American Chemical Society (ACS)
Date: 29-04-2019
Abstract: Although numerous studies have been conducted on the toxicity and biodistribution of AgNPs and corresponding ionic counterparts, it is still debatable whether the toxicity originates from the accumulation of particles within specific organs or is mediated by the dissolved Ag ions. To gain a better insight into the toxic mechanisms of AgNPs, two aggregation-induced emission fluorogens (AIEgens AIEgens-coated AgNPs and a fluorogenic Ag
Publisher: American Chemical Society (ACS)
Date: 19-11-2019
Abstract: Copper (Cu) is a key trace element for many biological processes, but there is little available information regarding its requirements and functions in critical life stages of marine bivalves, during which dramatic morphological and physiological changes occur. In this study, the ontogeny pattern of Cu accumulation across the life history of oysters was explored for the first time and the distributions of Cu in oysters at critical life stages (pe eliger and early settled spat) were in situ mapped by nanoscale secondary ion mass spectrometry (NanoSIMS) with high lateral resolution. We first demonstrated that the late pelagic stage to early settled stage was the critical stage requiring Cu during oyster development. NanoSIMS imaging revealed a significant elevation of intercellular Cu levels along with mitochondrial calcium overload and obvious structural degradation of mitochondria in velar cells at the pe eliger stage, implying the possible role of Cu in cell apoptosis of the velum during larval metamorphosis. Furthermore, an obvious enrichment of Cu together with calcium was observed in the nucleus and mitochondria of ciliated cells of gill tissue at the early settled stage. Their accumulation in the gill cells was significantly higher than that in the juveniles, indicating the potential role of Cu in sustaining the fast growth of the gill filament and the concomitant acceleration of energy metabolism during early benthic development. Our findings offer new insights into the understanding of the interactions between trace metals and marine bivalves.
Publisher: American Chemical Society (ACS)
Date: 24-07-2023
Publisher: American Chemical Society (ACS)
Date: 08-12-2017
Abstract: Determining the in situ localization of trace elements at high lateral resolution levels in the biological system is very challenging, but critical for our understanding of metal sequestration and detoxification. Here, the cellular and subcellular distributions of Cu and Zn in contaminated oysters of Crassostrea hongkongensis were for the first time mapped using nanoscale secondary ion mass spectrometry (nanoSIMS). Three types of metal-containing cells were revealed in the gill and mantle of oysters, including Cu-specific hemocytes, Cu and Zn-containing granular hemocytes, and Cu and Zn-containing calcium cells. Obvious intercellular distribution of Cu was found in the gill tissue, indicating the potential role of hemolymph in the transportation of Cu in oysters. The distribution of Cu showed a strong colocalization with sulfur and nitrogen in Cu-specific hemocyte and intercellular hemolymph. In the Cu and Zn-containing granular hemocytes and calcium cells, the co-occurrence of Cu and Zn with phosphorus and calcium was also found. Different relationships of distributions between Cu/Zn and macronutrient elements (nitrogen, sulfur and phosphorus) implied the differential metal complexation in oysters. Interestingly, quantitative analysis of the ratios of
Publisher: Elsevier BV
Date: 12-2021
Publisher: American Chemical Society (ACS)
Date: 11-08-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/C9SC06226B
Abstract: An AIE-active ratiometric probe for the first time achieved the long-term quantification of lysosomal pH during the medaka larva's caudal fin regeneration.
Publisher: Elsevier BV
Date: 08-2019
DOI: 10.1016/J.ENVPOL.2019.04.140
Abstract: The Pearl River Estuary (PRE) is the third largest estuary in China, where estuarine organisms are under metal stress at various biological levels. Based on the metal concentrations measured in oyster Crassostrea hongkongensis, we documented a change in dominance of metal contamination from Cd, Cr, Cu, Ni and Zn to Ag, Cd, Cu and Zn. In general, metal concentrations were higher in upstream stations and displayed a clear up-downstream gradient. Compared to the historical values, we noted the reductions in Cd, Cr and Ni concentrations, and the changing inputs due to evolving industrial activities were responsible for shaping the metal contamination profile in the PRE region. Along with metal concentrations, a suite of biomarkers was analyzed. Among the metals measured in the oyster tissues, Ag, Cd, Cu, Ni and Zn showed the strongest associations with pro-oxidant and oxidative stress responses (superoxide dismutase, lipid peroxidation and lysosomal membrane destabilization) and detoxification responses (glutathione and metallothionein), suggesting that the present metal contamination still exerts significant amount of stress in biota in the PRE. Metal contamination in estuaries in China is still severe compared to other countries, therefore continuous efforts should be taken to monitor the changing metal profiles with necessary control and remediation measures.
Publisher: American Chemical Society (ACS)
Date: 31-07-2020
Publisher: Elsevier BV
Date: 02-2011
Publisher: Elsevier BV
Date: 04-2011
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9EN00553F
Abstract: A fluorescence method was employed to monitor the real-time dissolution kinetics of zinc oxide nanoparticles.
Publisher: American Chemical Society (ACS)
Date: 11-05-2021
Publisher: Wiley
Date: 29-05-2019
Publisher: Springer Science and Business Media LLC
Date: 11-06-2013
Publisher: Elsevier BV
Date: 07-2019
DOI: 10.1016/J.ENVPOL.2019.04.003
Abstract: Metal flux measurements inform the mobility, potential bioavailability and risk of toxicity for metals in contaminated sediments and therefore is an important approach for sediment quality assessment. The binding and release of metals that contribute to the net flux is strongly influenced by the presence and behaviors of benthic organisms. Here we studied the effects of bioturbation on the mobility and efflux of metals from multi-metal contaminated sediments that inhabited by oligochaete worms or both worms and bivalves. Presence of bivalves enhanced the release of Mn, Co, Ni and Zn but not for copper and chromium, which is likely due to the high affinities of copper and chromium for the solid phase. Metals in the overlying water were primarily associated with fractions smaller than 10 kDa, and the fractionation of all metals were not affected by the presence of the bivalve. Metal fluxes attributed to different processes were also distinguished, and the bioturbation induced effluxes were substantially higher than the diffusive effluxes. Temporal variabilities in the total net effluxes of Mn, Co, Ni and Zn were also observed and were attributed to the biological activities of the bivalves. Overall, the present study demonstrated that the response of different metals to the same bioturbation behavior was different, resulting in distinct mobility and fate of the metal contaminants.
Publisher: Elsevier BV
Date: 2018
DOI: 10.1016/J.ENVPOL.2017.09.040
Abstract: The behaviour of arsenic (As) from geogenic soil exposed to aerobic conditions is critical to predict the impact of As on the environment, which processes remain unresolved. The current study examined the depth profile of As in geologically derived subsoil cores from Hong Kong and investigated the mobilization, plant availability, and bioaccessibility of As in As-contaminated soil at different depths (0-45.8 m). Results indicated significant heterogeneity, with high levels of As in three layers of soil reaching up to 505 mg/kg at a depth of 5 m, 404 mg/kg at a depth of 15 m, and 1510 mg/kg at a depth of 27-32 m. Arsenic in porewater s les was <11.5 μg/L in the study site. X-ray absorption spectroscopy (XAS) indicated that main As species in soil was arsenate (As(V)), as adsorbed fraction to Fe oxides (41-69% on goethite and 0-8% on ferrihydrite) or the mineral form scorodite (30-57%). Sequential extraction procedure demonstrated that 0.5 ± 0.4% of As was exchangeable. Aerobic incubation experiments exhibited that a very small amount (0.14-0.48 mg/kg) of As was desorbed from the soil because of the stable As(V) complex structure on abundant Fe oxides (mainly goethite), where indigenous microbes partly (59 ± 18%) contributed to the release of As comparing with the sterilized control. Furthermore, no As toxicity in the soil was observed with the growth of ryegrass. The bioaccessibility of As was <27% in the surface soil using simplified bioaccessibility extraction test. Our systematic evaluation indicated that As in the geogenic soil profile from Hong Kong is relatively stable exposing to aerobic environment. Nevertheless, children and workers should avoid incidental contact with excavated soil, because high concentration of As was present in the digestive solution (<0.1-268 μg/L).
Publisher: American Chemical Society (ACS)
Date: 16-12-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1EN00146A
Abstract: Nano-ZnO with low extracellular dissolution stimulates high lysosomal accumulation and ROS production and exhibits greater cytotoxicity than dissolved Zn 2+ .
Publisher: American Chemical Society (ACS)
Date: 22-06-2021
Abstract: Nanoparticles (NPs) for delivering chemotherapeutic drugs are now in clinical trials, and cellular uptake of NPs plays an important role in determining the drug delivery efficiency. Herein, we reported that the bioaccumulation and internalization of NPs were governed by the cell cycle. Specifically, we found that the bioaccumulation of NPs was more favored in the G 2 /M stages, followed by the S and G 0 /G 1 stages. We demonstrated that three key parameters-clathrin-mediated endocytosis capacity, algal cell membrane permeability, and exopolymer substance (EPS) thickness-were critical in the bioaccumulation of NPs during the cell cycling process. Over the 24-h average duration of cell cycle, clathrin-mediated endocytosis capacity was much higher at the S stage than that at the G 0 /G 1 and G 2 /M stages. Besides, cell membrane permeability was measured to be higher in S and G 2 /M stages while the lowest in G 0 /G 1 stage. We have also identified the change of EPS thickness during the 24-h cell cycle. Transition from G 0 /G 1 to S and G 2 /M induced the attenuation in EPS thickness, and the thinnest EPS was found at the end of mitosis. The cell cycle control NPs internalization were further verified by exposing Ag nanoparticles to algae at different cell cycle stages, confirming the important roles of EPS thickness and cell cycle control in the dynamic internalization processes. The present study highlights the important roles of cell cycle controlling the NPs bioaccumulation and internalization, with possible implications in maximizing NPs internalization efficiency while reducing the cost.
Publisher: American Chemical Society (ACS)
Date: 20-11-2018
Abstract: Release of silver ions (Ag
Publisher: American Chemical Society (ACS)
Date: 02-10-2023
Publisher: Elsevier BV
Date: 11-2018
DOI: 10.1016/J.AQUATOX.2018.09.011
Abstract: Copper (Cu) can cause oxidative stress and inflammatory responses, and there is arising evidence between Cu toxicity and lipid disturbance. In this study, we examined the relationships between Cu exposure and lipid metabolism in an estuarine oyster (Crassostrea hongkongensis) and aimed to understand the effects and resilience strategies of Cu on oyster metabolism. We exposed the oysters to waterborne Cu (10 and 50 μg/L) and measured the physiological changes (condition index and clearance rate), lipid accumulation and lipid peroxidation in the oysters. We found more altered lipid responses in oysters exposed to a lower Cu concentration (10 μg/L), and speculated that oysters exposed to 50 μg/L may upregulate the defenses. We further evaluated the changes in lipidome profiling of the Cu-exposed oysters in aspects of membrane dynamics, lipid signaling and energy metabolism. We documented the phospholipid remodeling as well as quick modulation in inflammatory responses and extensive vesicle formation for subcellular compartmentalization and autophagosome formation, as well as the possible impacts on mitochondrial bioenergetics in the Cu-exposed oysters. The lipidomics approach provided a comprehensive lipid profile of possible alteration by Cu exposure. In combination with other omics approaches, it may be possible to elucidate the pathways and mechanisms in stress acclimation and resilience associated between Cu contamination and lipid metabolism.
Publisher: American Chemical Society (ACS)
Date: 03-11-2021
Publisher: American Chemical Society (ACS)
Date: 05-11-2021
DOI: 10.1021/ACSSENSORS.1C01821
Abstract: Extracellular polymeric substances (EPS) are produced by many microorganisms and play an essential role in physiological systems such as nutrient storage and stress resistance. Besides, EPS show great potential in biomedical and therapeutic applications due to their biocompatibility and biodegradability. In situ noninvasive monitoring of the EPS produced by microorganisms is thus critical but has not yet been achieved. Herein, we developed a novel aggregation-induced emission (AIE) active nanoprobe enabling in situ visualization of the EPS distribution produced by various microorganisms (cyanobacteria, yeast, freshwater, and marine phytoplankton). The synthesized AIE-active nanoprobe displayed excellent specificity and precision for the staining of EPS, as well as strong photostability, showing great advantage in sensing the EPS in living organisms. With the application of this novel probe, the three-dimensional (3D) framework of EPS distribution was visualized under different environmental conditions (temperature, light intensity, nutrition, and pH). The EPS distribution was found to correlate significantly with the metal tolerance and cyanobacterial photosynthesis capability. Collectively, this study proposed an AIE-active nanoprobe for visualizing the EPS distribution and quantifying the EPS thickness/volume, and has significant implications in understanding the physiological functions of microorganisms.
Publisher: American Chemical Society (ACS)
Date: 25-05-2021
No related grants have been discovered for Wenxiong Wang.