ORCID Profile
0000-0003-1742-6350
Current Organisations
University of Adelaide
,
Northern Technical University
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Publisher: Springer Science and Business Media LLC
Date: 07-02-2018
DOI: 10.1186/S13104-018-3223-Y
Abstract: This study aimed to provide a rapid, accurate and cost-effective diagnostic real time polymerase chain reaction-high resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single commercial dairy farm in South Australia. One set of genus-level universal primers was designed targeting the 16S ribosomal RNA gene. Real time PCR-HRM analysis was able to identify and distinguish between five different mollicutes, namely A. laidlawii , M. arginini , M. bovirhinis , M. bovis and uncultured Mycoplasma . Results were confirmed through sequencing. Our developed assay provides rapid and accurate screening for Mycoplasma mastitis detection.
Publisher: IOP Publishing
Date: 04-2023
DOI: 10.1088/1755-1315/1158/5/052017
Abstract: The current study was carried out from October 15, 2021, to June 15, 2022, in the area of animal production at the Agricultural Technical College, Northern Technical University, Mosul, Iraq. 24 Awassi ewes between the ages of 2-3, were employed in the study and split into two groups. In the first group, 12 ewes had winter lambs in December–January and 12 ewes had spring lambs in March–April. This was required to determine how the season affected some productive and biochemical blood features. The milk yield and its components of Awassi sheep, and some biochemical characteristics, as well as the number of somatic cells in milk, the estrus of the ewes were standardized using vaginal sponges. The season had a noticeable effect on milk production, as ewes with spring births produced significantly more milk in the second, fourth, eighth, and tenth weeks after birth. In the eighth and tenth week of the experiment, non-fat solids were consumed. Except for the fourth week of the trial, the number of somatic cells in the milk of spring-born ewes increased significantly (P0.05) in all other weeks of the study. The proportions of fat, protein, and solid non-fat percentages increased significantly. In addition to an increase in the number of somatic cells increased significantly and the incidence of mastitis in the milk of ewes with spring births.
Publisher: University of Mosul
Date: 12-2022
Publisher: IOP Publishing
Date: 04-2023
DOI: 10.1088/1755-1315/1158/5/052004
Abstract: The objective of this review is to compile and assess the findings of several studies on the use of Rocket (Eruca sativa) as feed additives or alternatives to fish nutrition. Antioxidant glucosinolates, flavonoids, and vitamins A and C are present in this plant, which has been shown to improve growth metrics and blood characteristics, according to the findings of the scientists. Effective oxidation against stress, the generation of free radicals, and pathogenic microorganisms, which enhances the health of fish and thus promotes growth and food utilization. In addition to its medical value as an alternative to antibiotics with negative side effects.
Publisher: IOP Publishing
Date: 04-2023
DOI: 10.1088/1755-1315/1158/5/052010
Abstract: The current study was carried out from October 15, 2021, to June 15, 2022, in the area of animal production at the Agricultural Technical College, Northern Technical University, Mosul, Iraq. 24 Awassi ewes between the ages of 2-3, were employed in the study and split into two groups. In the first group, 12 ewes had winter lambs in December–January and 12 ewes had spring lambs in March–April. This was required to determine how the season affected some productive and biochemical blood features. The milk yield and its component of Awassi sheep, in addition to measuring the weights of the ewes and lambs, the weight gain every two weeks, final weight gain, relative growth rate, and some biochemical characteristics, as well as the number of somatic cells in milk, the estrus of the ewes was standardized using vaginal sponges. The results revealed that the body weight of ewes with spring births in the second, fourth, sixth, eighth, tenth, and twelfth weeks was significantly (P0.05) greater than that of ewes with spring births. There was a substantial (P0.05) rise in the average weight of lambs with spring births in the tenth and twelfth weeks of the trial, as well as a significant increase in the relative growth rate of lambs with spring births in the tenth week. In addition, the total rate of weight gains increased. The season had a noticeable effect on milk production, as ewes with spring births produced significantly more milk in the second, fourth, eighth, and tenth weeks after birth. In the eighth and tenth week of the experiment, nonfat solids were consumed. Except for the fourth week of the trial, the number of somatic cells in the milk of spring-born ewes increased significantly (P0.05) in all other weeks of the study. The weights of ewes and lambs, as well as the total weight gain, increased during the spring. furthermore, the proportions of fat, protein, and solid non-fat percentage increased significantly. In addition to an increase in the number of somatic cells and the prevalence of mastitis, the milk of spring-born ewes is non-fatty.
Publisher: Egypts Presidential Specialized Council for Education and Scientific Research
Date: 07-2023
Publisher: Oxford University Press (OUP)
Date: 09-2022
DOI: 10.1111/JAM.15655
Abstract: The aim of this study was to assess a phage-displayed MilA protein of Myc. bovis in an indirect ELISA for the detection of Myc. bovis antibodies in milk s les. The desired sequence of milA gene was synthesized and cloned into pCANTAB-F12 phagemid vector. The expression of the MilA on the phage surface was confirmed by Western blotting. The recombinant phage was used in the development of an indirect ELISA to detect Myc. bovis antibodies in milk s les. There was a significant agreement between the results of phage-based ELISA and recombinant GST-MilA ELISA for the detection of Myc. bovis antibodies in milk s les. The inexpensive and convenient phage-based ELISA can be used instead of recombinant protein eptide ELISA as an initial screening of Myc. bovis-associated mastitis. Mastitis associated with Myc. bovis is a continuous and serious problem in the dairy industry. Sero-monitoring of Myc. bovis infection cases are one of the key factors for surveillance of the infections in dairy farms. Despite the existence of some commercially serological assays for Myc. bovis antibodies, they have some limitations regarding their sensitivity and availability. The development of accurate diagnosis tools could contribute to control programmes of Myc. bovis-associated mastitis in the dairy herds.
Publisher: Kafkas University
Date: 2020
Publisher: Springer Science and Business Media LLC
Date: 25-11-2017
Publisher: Springer Science and Business Media LLC
Date: 02-04-2018
Publisher: The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS
Date: 02-06-2020
DOI: 10.3906/VET-1811-62
Publisher: Elsevier BV
Date: 06-2018
DOI: 10.1016/J.VETMIC.2018.04.004
Abstract: Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have recently emerged as a significant cause of bovine mastitis worldwide. Here we describe the isolation of MRCoNS from cases of bovine mastitis from a single dairy farm in Australia. Fourteen CoNS isolates were identified as MRCoNS on the basis of having an oxacillin MIC of ≥0.5 μg/mL. The isolates were speciated as S. chromogenes (n = 1) S. fleurettii (n = 1), S. haemolyticus (n = 2), S. sciuri (n = 5), S. simulans (n = 1) S. succinus (n = 2) and S. xylosus (n = 2). Five of the isolates (S. fleuretti, S. haemolyticus S. sciuri and two S. succinus) were mecA-positive. We also detected a previously described S. sciuri mecA homolog in four oxacillin-resistant S. sciuri isolates. The remainder of the putative MRCoNS did not contain any mecA-related resistance determinants in their genomes. Comparative genomic analysis of three previously published S. sciuri isolates, from humans, a squirrel and a cereal crop (rice), and a representative isolate from our study demonstrated clustering and a high degree of genetic homogeneity (>95%), suggesting S. sciuri has low host specificity. In conclusion, CoNS, in particular S. sciuri, may act as a reservoir for SCCmec elements that can easily be spread between different host species by direct cross-infection.
Publisher: Egypts Presidential Specialized Council for Education and Scientific Research
Date: 07-2023
No related grants have been discovered for Abd Al-Bar Al-Farha.