ORCID Profile
0000-0002-4809-9826
Current Organisation
University of California, San Diego
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Publisher: American Chemical Society (ACS)
Date: 27-10-2017
Publisher: American Chemical Society (ACS)
Date: 25-08-2017
Publisher: American Chemical Society (ACS)
Date: 20-11-2020
DOI: 10.1021/ACSBIOMATERIALS.0C01313
Abstract: Porous silicon (pSi) nanoparticles are loaded with Immunoglobulin A-2 (IgA2) antibodies, and the assembly is coated with pH-responsive polymers on the basis of the Eudragit family of enteric polymers (L100, S100, and L30-D55). The temporal release of the protein from the nanocomposite formulations is quantified following an in vitro protocol simulating oral delivery: incubation in simulated gastric fluid (SGF at pH 1.2) for 2 h, followed by a fasting state simulated intestinal fluid (FasSIF at pH 6.8) or phosphate buffer solution (PBS at pH 7.4). The nanocomposite formulations display a negligible release in SGF, while more than 50% of the loaded IgA2 is released in solutions at a pH of 6.8 (FasSIF) or 7.4 (PBS). Between 21 and 44% of the released IgA2 retains its functional activity. A capsule-based system is also evaluated, where the IgA2-loaded particles are packed into a gelatin capsule and the capsule is coated with either EudragitL100 or EudragitS100 polymer for a targeted release in the small intestine or the colon, respectively. The capsule-based formulations outperform polymer-coated nanoparticles in vitro , preserving 45-54% of the activity of the released protein.
Publisher: Wiley
Date: 12-07-2017
Abstract: There is an urgent need for new materials to treat bacterial infections. In order to improve antibacterial delivery, an anti‐infective nanomaterial is developed that utilizes two strategies for localization: i) a biodegradable nanoparticle carrier to localize therapeutics within the tissue, and ii) a novel tandem peptide cargo to localize payload to bacterial membranes. First, a library of antibacterial peptides is screened that combines a membrane‐localizing peptide with a toxic peptide cargo and discovers a tandem peptide that displays synergy between the two domains and is able to kill Pseudomonas aeruginosa at sub‐micromolar concentrations. To apply this material to the lung, the tandem peptide is loaded into porous silicon nanoparticles (pSiNPs). Charged peptide payloads are loaded into the pores of the pSiNP at ≈30% mass loading and ≈90% loading efficiency using phosphonate surface chemistry. When delivered to the lungs of mice, this anti‐infective nanomaterial exhibits improved safety profiles over free peptides. Moreover, treatment of a lung infection of P. aeruginosa results in a large reduction in bacterial numbers and markedly improves survival compared to untreated mice. Collectively, this study presents the selection of a bifunctional peptide‐based anti‐infective agent and its delivery via biodegradable nanoparticles for application to an animal model of lung infection.
Publisher: Wiley
Date: 12-11-2019
Publisher: American Chemical Society (ACS)
Date: 27-01-2017
Publisher: American Chemical Society (ACS)
Date: 16-01-2018
Publisher: American Chemical Society (ACS)
Date: 17-01-2018
Publisher: American Chemical Society (ACS)
Date: 24-01-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/C9NR08474F
Abstract: Synthetic DNA-based oligonucleotides are loaded into porous silicon nanoparticles (pSiNPs) and incorporated into nanofibers of poly(lactide-co-glycolide) (PLGA), poly-l-lactic acid (PLA), or polycaprolactone (PCL). The resulting hybrid nanofibers are characterized for their ability to release the functional oligonucleotide payload under physiologic conditions. Under temperature and pH conditions mimicking physiological values, the PLGA-based nanofibers release >80% of their DNA cargo within 5 days, whereas the PLA and PCL-based fibers require 15 days to release >80% of their cargo. The quantity of DNA released scales with the quantity of DNA-loaded pSiNPs embedded in the nanofibers mass loadings of between 2.4 and 9.1% (based on mass of DNA-pSiNP construct relative to mass of polymer composite) are investigated. When a responsive DNA-based nanodevice (i.e. molecular beacon) is used as a payload, it retains its functionality during the release period, independent of the polymer used for the formation of the nanofibers.
Publisher: Wiley
Date: 26-08-2009
Publisher: American Chemical Society (ACS)
Date: 09-2021
Publisher: American Chemical Society (ACS)
Date: 24-04-2020
Publisher: American Chemical Society (ACS)
Date: 14-03-2022
Publisher: American Chemical Society (ACS)
Date: 28-04-2017
Publisher: Wiley
Date: 24-01-2018
Publisher: American Chemical Society (ACS)
Date: 22-01-2021
Publisher: American Chemical Society (ACS)
Date: 26-01-2018
Publisher: American Chemical Society (ACS)
Date: 27-07-2018
Publisher: Wiley
Date: 11-05-2020
Location: United States of America
No related grants have been discovered for Michael Sailor.