Lew Zipin

The contemporary challenge of activism as curriculum work

Publisher: Informa UK Limited

Date: 28-02-2022

DOI: 10.1080/00220620.2020.1866508

A bacterial pioneer produces cellulase complexes that persist through community succession

Publisher: Springer Science and Business Media LLC

Date: 06-11-2018

DOI: 10.1038/S41564-017-0052-Z

Abstract: Cultivation of microbial consortia provides low-complexity communities that can serve as tractable models to understand community dynamics. Time-resolved metagenomics demonstrated that an aerobic cellulolytic consortium cultivated from compost exhibited community dynamics consistent with the definition of an endogenous heterotrophic succession. The genome of the proposed pioneer population, ‘ Candidatus Reconcilibacillus cellulovorans’, possessed a gene cluster containing multidomain glycoside hydrolases (GHs). Purification of the soluble cellulase activity from a 300litre cultivation of this consortium revealed that ~70% of the activity arose from the ‘ Ca . Reconcilibacillus cellulovorans’ multidomain GHs assembled into cellulase complexes through glycosylation. These remarkably stable complexes have supramolecular structures for enzymatic cellulose hydrolysis that are distinct from cellulosomes. The persistence of these complexes during cultivation indicates that they may be active through multiple cultivations of this consortium and act as public goods that sustain the community. The provision of extracellular GHs as public goods may influence microbial community dynamics in native biomass-deconstructing communities relevant to agriculture, human health and biotechnology.

Opening school walls to funds of knowledge

Publisher: Routledge

Date: 10-05-2023

DOI: 10.4324/9781003369851-6

Introduction - knowledge-and-ethics: strong curriculum values both, together

Publisher: Springer Science and Business Media LLC

Date: 04-2020

DOI: 10.1007/S41297-019-00091-3

Identification of the O-GalNAcylation site(s) on FOXA1 catalyzed by ppGalNAc-T2 enzyme in vitro

Publisher: Elsevier BV

Date: 06-2019

DOI: 10.1016/J.BBRC.2019.04.146

Abstract: FOXA1 functions as a pioneer factor of transcriptional regulation that binds to specific sites in the chromatin and recruits other transcription factors, promoting the initiation of gene transcription and mediating the regulation of downstream target gene expression. FOXA1 was reported to facilitate or reprogram ERα binding, thus playing a key function in breast cancer progression. Our previous results indicated that the O-linked N-acetylgalactosamine (O-GalNAc) modification of FOXA1 plays a potentially significant role in the ERα transcription network. However, further investigations are needed to identify the specific mechanism of modification and the specific glycosylation sites on FOXA1. In this study, we first suggested that FOXA1 could be O-GalNAcylated by ppGalNAc-T2 in vitro. By iding and expressing recombinant FOXA1 as three segments, two O-GalNAcylation sites were found on FOXA1, both located at the C-terminal of the protein. Then, synthesized peptides, including the predicted O-GalNAc sites in the C-terminus of FOXA1, were used in a vitro reaction, and peptides mutated at the predicted O-GalNAc sites were employed as controls. Through an ESI-MS assay, S354 and S355 were identified as probable O-GalNAcylation sites on FOXA1. Additionally, we performed ESI-ETD-MS/MS analysis of the full-length O-GalNAcylated FOXA1 protein and identified S355 as the O-GalNAc modification site on FOXA1, consistent with the peptide reaction. In conclusion, our results demonstrated that FOXA1 can be O-GalNAcylated by ppGalNAc-T2 at S355 in vitro. These results will provide new insights for studying the role of O-GalNAcylation in the development of breast cancer.

CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation

Publisher: Public Library of Science (PLoS)

Date: 29-08-2018

DOI: 10.1371/JOURNAL.PBIO.2005817

The contemporary challenge of activism as curriculum work

Publisher: Routledge

Date: 08-09-2023

DOI: 10.4324/9781003451631-7

Building curriculum knowledge work around community-based “problems that matter”: let’s dare to imagine

Publisher: Springer Science and Business Media LLC

Date: 04-2020

DOI: 10.1007/S41297-019-00096-Y

Hyposialylated IgG activates endothelial IgG receptor FcγRIIB to promote obesity-induced insulin resistance

Publisher: American Society for Clinical Investigation

Date: 27-11-2017

DOI: 10.1172/JCI89333

Line segment based structure and motion from two views

Publisher: SPIE

Date: 10-02-2011

DOI: 10.1117/12.872346

Aggravating Students’ Structural Vulnerabilities: Cruel Miseries of Selection for ‘Success’ in Schools with Power-Marginalised Intake

Publisher: Springer Science and Business Media LLC

Date: 09-2021

DOI: 10.1007/S43151-021-00054-4

A Novel Germline Variant in CSF3R Reduces N-Glycosylation and Exerts Potent Oncogenic Effects in Leukemia

Publisher: American Association for Cancer Research (AACR)

Date: 13-12-2018

DOI: 10.1158/0008-5472.CAN-18-1638

Abstract: This study reveals the critical importance of membrane-proximal N-linked glycosylation of CSF3R for the maintenance of ligand dependency in leukemia

University Of South Australia

Location: Australia

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The University Of Canberra

Location: Australia

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Université De Picardie Jules Verne

Location: France

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Université De Bourgogne

Location: France

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Victoria University

Location: Australia

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University Of Wisconsin Madison

Location: United States of America

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Linkage Projects - Grant ID: LP0454869

Start Date: 07-2004

End Date: 06-2012

Amount: $363,828.00

Funder: Australian Research Council

Discovery Projects - Grant ID: DP120101492

Start Date: 03-2012

End Date: 12-2017

Amount: $180,000.00

Funder: Australian Research Council

Linkage Projects - Grant ID: LP100200841

Start Date: 05-2011

End Date: 05-2016

Amount: $386,000.00

Funder: Australian Research Council