ORCID Profile
0000-0002-1830-9917
Current Organisations
Monash University
,
University of Melbourne
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Publisher: Wiley
Date: 10-05-2016
DOI: 10.1111/ENE.13020
Abstract: Investigation of the relationship between mitochondrial DNA (mtDNA) variants and Parkinson disease (PD) remains an issue awaiting more supportive evidence. Moreover, an affirming cellular model study is also lacking. The index mtDNA variants and their defining mitochondrial haplogroup were determined in 725 PD patients and 744 non-PD controls. Full-length mtDNA sequences were also conducted in 110 cases harboring various haplogroups. Cybrid cellular models, composed by fusion of mitochondria-depleted rho-zero cells and donor mitochondria, were used for a rotenone-induced PD simulation study. Multivariate logistic regression analysis revealed that subjects harboring the mitochondrial haplogroup B5 have resistance against PD (odds ratio 0.50, 95% confidence interval 0.32-0.78 P = 0.002). Furthermore, a composite mtDNA variant group consisting of A10398G and G8584A at the coding region was found to have resistance against PD (odds ratio 0.50, 95% confidence interval 0.33-0.78 P = 0.001). In cellular studies, B4 and B5 cybrids were selected according to their higher resistance to rotenone, in comparison with cybrids harboring other haplogroups. The B5 cybrid, containing G8584A/A10398G variants, showed more resistance to rotenone than the B4 cybrid not harboring these variants. This is supported by findings of low reactive oxygen species generation and a low apoptosis rate in the B5 cybrid, whereas a higher expression of autophagy was observed in the B4 cybrid particularly under medium dosage and longer treatment time with rotenone. Our studies, offering positive results from clinical investigations and cybrid experiments, provide data supporting the role of variant mtDNA in the risk of PD.
Publisher: No publisher found
Date: 2013
Publisher: Elsevier BV
Date: 05-2015
DOI: 10.1016/J.MITO.2015.02.006
Abstract: Adult mesenchymal stem cell (MSC)-conducted mitochondrial transfer has been recently shown to rescue cellular bioenergetics and prevent cell death caused by mitochondrial dysfunction. Wharton's jelly-derived MSCs (WJMSCs) harvested from postpartum umbilical cords are an accessible and abundant source of stem cells. This study aimed to determine the capability of WJMSCs to transfer their own mitochondria and rescue impaired oxidative phosphorylation (OXPHOS) and bioenergetics caused by mitochondrial DNA defects. To do this, WJMSCs were co-cultured with mitochondrial DNA (mtDNA)-depleted ρ(0) cells and the recapture of mitochondrial function was evaluated. WJMSCs were shown to be capable of transferring their own mitochondria into ρ(0) cells and underwent interorganellar mixture within these cells. Permissive culture media (BrdU-containing and pyruvate- and uridine-free) sieved out a survival cell population from the co-cultured WJMSCs (BrdU-sensitive) and ρ(0) cells (pyruvate/uridine-free). The survival cells had mtDNA identical to that of WJMSCs, whereas they expressed cellular markers identical to that of ρ(0) cells. Importantly, these ρ(0)-plus -WJMSC-mtDNA (ρ(+W)) cells recovered the expression of mtDNA-encoded proteins and exhibited functional oxygen consumption and respiratory control, as well as the activity of electron transport chain (ETC) complexes I, II, III and IV. In addition, ETC complex V-inhibitor-sensitive ATP production and metabolic shifting were also recovered. Furthermore, cellular behaviors including attachment-free proliferation, aerobic viability and OXPHOS-reliant cellular motility were also regained after mitochondrial transfer by WJMSCs. The therapeutic effect of WJMSCs-derived mitochondrial transfer was able to stably sustain for at least 45 passages. In conclusion, this study suggests that WJMSCs may serve as a potential therapeutic strategy for diseases linked to mitochondrial dysfunction through the donation of healthy mitochondria to cells with genetic mitochondrial defects.
Publisher: SAGE Publications
Date: 03-1992
DOI: 10.1057/FR.1992.7
Publisher: American Diabetes Association
Date: 13-09-2012
DOI: 10.2337/DB11-1369
Abstract: Both the coding and control regions of mitochondrial DNA (mtDNA) play roles in the generation of diabetes however, no studies have thoroughly reported on the combined diabetogenic effects of variants in the two regions. We determined the mitochondrial haplogroup and the mtDNA sequence of the control region in 859 subjects with diabetes and 1,151 normoglycemic control subjects. Full-length mtDNA sequences were conducted in 40 subjects harboring specific diabetes-related haplogroups. Multivariate logistic regression analysis with adjustment for age, sex, and BMI revealed that subjects harboring the mitochondrial haplogroup B4 have significant association with diabetes (DM) (odds ratio [OR], 1.54 [95% CI 1.18–2.02] P & 0.001), whereas subjects harboring D4 have borderline resistance against DM generation (0.68 [0.49–0.94] P = 0.02). Upon further study, we identified an mtDNA composite group susceptible to DM generation consisting of a 10398A allele at the coding region and a polycytosine variant at nucleotide pair 16184–16193 of the control region, as well as a resistant group consisting of C5178A, A10398G, and T152C variants. The OR for susceptible group is 1.31 (95% CI 1.04–1.67 P = 0.024) and for the resistant group is 0.48 (0.31–0.75 P = 0.001). Our study found that mtDNA variants in the coding and control regions can have combined effects influencing diabetes generation.
Publisher: Informa UK Limited
Date: 03-09-2017
Publisher: Routledge
Date: 03-06-2020
Publisher: Springer International Publishing
Date: 2019
Publisher: Oxford University Press (OUP)
Date: 03-1995
Publisher: Routledge
Date: 03-06-2020
Publisher: Edinburgh University Press
Date: 03-2007
Abstract: This article discusses the current ‘popularity’ of trauma research in the Humanities and examines the ethics and politics of trauma theory, as exemplified in the writings of Caruth and Felman and Laub.Written from a position informed by Laplanchian and object relations psychoanalytic theory, it begins by examining and offering a critique of trauma theory's model of subjectivity, and its relations with theories of referentiality and representation, history and testimony. Next, it proposes that although trauma theory's subject matter—the sufferings of others—makes critique difficult, the theory's politics, its exclusions and inclusions, and its unconscious drives and desires are as deserving of attention as those of any other theory. Arguing that the political and cultural contexts within which this theory has risen to prominence have remained largely unexamined, the article concludes by proposing that trauma theory needs to act as a brake against rather than as a vehicle for cultural and political Manicheanism.
Publisher: Informa UK Limited
Date: 2001
Publisher: Palgrave Macmillan UK
Date: 2007
Publisher: Oxford University Press (OUP)
Date: 09-1991
Publisher: Informa UK Limited
Date: 06-2012
Publisher: SAGE Publications
Date: 28-06-2013
Abstract: For the psychoanalyst D.W. Winnicott, living takes place at the intersection of inner and outer worlds, in a space riven by destructiveness and enlivened by love, play and repair. Working with Winnicott’s understandings of empathy and play, this essay explores creative writing’s prompting of a recognition of the not-yet-past, as it suffuses the place where we live. This essay focuses on the rancorous debates among historians and critics that followed the publication of Kate Grenville’s The Secret River, a novel about Australia’s settler-colonial past. After pointing to the centrality of empathy within these debates, the essay examines Winnicott’s exploration of destructiveness as the precursor to empathy and reparation. The essay continues by examining the different modes of acknowledged and disavowed empathy associated with historiography and fiction, exploring, in particular, The Secret River’s engagement of empathy. The essay concludes by suggesting that Grenville’s work of ‘historical’ fiction ‘contributes’ (in Winnicott’s terms) by provoking an awareness that the violence that was part of Australia’s settler-colonialist history cannot be simply consigned to the past but lives on – and requires recognition – in the place that is Australia today.
Publisher: SAGE Publications
Date: 17-09-2020
Abstract: This essay responds to Astrid Erll’s question about what it might mean to do memory studies in different parts of the world. We offer a response from the perspective of three researchers based in Australia. Focused on a season-opening gala performance, a photographic series, a site-specific protest, and a film that takes a choir from Central Australia to Germany, the essay tracks the emergence, in culture, of something we term the ‘here-now’. The essay argues that this ‘here-now’ belongs neither to historical temporality’s linear time-line, nor to the cosmology of an unsullied Indigenous culture – and cannot easily be addressed in the language of memory studies. Taking our lead from four case studies, we try to find words for what it is that the ‘here-now’ makes present, as it emerges in the artworks and events we discuss. We find that the ‘here-now’s’ ordering of place/time insistently evokes a yet-to-be realized Australia, while prompting recognition of the hard truths that still stand in its way.
Publisher: No publisher found
Date: 2013
Publisher: Informa UK Limited
Date: 28-01-2010
Publisher: University of Chicago Press
Date: 09-2002
DOI: 10.1086/340889
Publisher: Oxford University Press (OUP)
Date: 05-1985
Publisher: SAGE Publications
Date: 28-06-2013
Publisher: Wiley
Date: 12-06-2013
DOI: 10.1016/J.FEBSLET.2013.06.003
Abstract: Demonstration of the function of the Arabidopsis thaliana acyl-CoA:diacylglycerol acyltransferase 2 (AtDGAT2) has remained elusive despite biochemical testing of genetic mutants and overexpression lines. We show that transiently expressed AtDGAT2 in the Nicotiana benthamiana leaf resulted in an increase in triacylglycerol twice as great as the increase observed following parallel expression of AtDGAT1. AtDGAT2 showed higher conversion from labeled diacylglycerol to triacylglycerol compared to AtDGAT1, and was acyl-CoA dependent. In addition, AtDGAT2 had different acyl-CoA substrate preference than AtDGAT1. These results allow us to conclude that AtDAGT2 is a functional acyl-CoA:diacylglycerol acyltransferase enzyme that can catalyse substantial increase in TAG synthesis.
Publisher: Informa UK Limited
Date: 03-09-2018
Publisher: Informa UK Limited
Date: 03-09-2018
Publisher: Informa UK Limited
Date: 11-2011
Publisher: Oxford University Press (OUP)
Date: 03-2005
DOI: 10.1093/HWJ/DBI012
Publisher: SAGE Publications
Date: 2008
Abstract: This article examines the opportunities and risks afforded by the consolidation of memory research into the subject area of `memory studies'. Debates about memory culture outside the academy and within academic memory research have hinged on its perceived over-personalization of the political. However, memory research is often informed by a broader ethical turn that understands itself to be transforming politics. The article argues that this split results in part from the over-generalizations produced by the travelling concepts of a transdisciplinary memory studies. It concludes that the politics of memory culture and of memory research might be best analysed and practiced within the disciplines and by means of the research methods from which memory studies borrows.
Publisher: Oxford University Press (OUP)
Date: 03-2005
DOI: 10.1093/HWJ/DBI011
Publisher: Fordham University Press
Date: 2010
Publisher: MDPI AG
Date: 22-01-2014
DOI: 10.3390/IJMS15011625
Publisher: Oxford University Press (OUP)
Date: 06-2001
Publisher: Peter Lang
Publisher: Bloomsbury Academic
Date: 2000
Publisher: SensePublishers
Date: 2013
Publisher: Bloomsbury Academic
Date: 2000
Publisher: Hindawi Limited
Date: 2012
DOI: 10.1155/2012/824275
Abstract: Mitochondrial DNA (mtDNA) haplogroups may contribute to the development of aging-related diseases. A reliable in vitro cellular system for investigating the physiologic significance of mtDNA haplogroups is essential. This study aims to construct and characterize a series of cybrid cell lines harboring variant mtDNA haplogroups collected from healthy Taiwanese volunteers. Cybrid cells harboring different mtDNA haplogroups like B4a, B4b, B4c, B4d, B5, R, F1a, F2, D4e, D4a, D5b, D5a, E, M8, C, and N9a were prepared. Luminex 1000 and full-length mtDNA sequencing were used to confirm that mtDNA haplogroups of transmitochondrial cybrids were identical to their original donors. Cybrid B4b had a significantly lower oxygen consumption rate and higher mitochondrial membrane potential compared to F1a, B5, D5a, D4a, and N9a but had more susceptibility to H 2 O 2 -induced oxidative stress than cybrid F1a, D4a, and N9a. Cybrid N9a had better oxygen consumption and H 2 O 2 -challenged viability compared to B4b, F1a, B5, D5a, and D4a. A series of cybrid cells harboring the main haplogroups of the Taiwanese population with ethnic Chinese background has been developed in vitro . With this mtDNA haplogroup population, the underlying mechanisms of aging-related diseases may be better understood, and therapeutic interventions can be accelerated.
Publisher: S. Karger AG
Date: 1996
DOI: 10.1159/000174050
Abstract: Multifrequency bio-electrical impedance analysis (MFBIA) was evaluated as a technique for monitoring changes in extracellular and total body water (ECW and TBW, respectively) of 15 subjects during dialysis. Dilution analysis, using deuterium oxide and sodium bromide, was also performed on each subject before dialysis so that prediction equations for ECW and TBW based on the MFBIA measures could be developed. These prediction equations were then used to estimate water compartment volume changes during dialysis and compared with volumetric measures of the dialysate removed. The results show that MFBIA does not accurately measure ECW and TBW changes during dialysis. The MFBIA measures tend to overestimate the changes and are not sufficiently precise to be clinically useful.
Publisher: Wiley
Date: 09-1993
Publisher: Routledge
Date: 29-09-2017
Publisher: Routledge
Date: 03-12-2007
Publisher: Peter Lang UK
Date: 12-05-2011
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Susannah Radstone.