ORCID Profile
0000-0001-6821-6690
Current Organisation
University of Nottingham
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Publisher: Frontiers Media SA
Date: 09-04-2015
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422266
Abstract: Supplementary Figure 1: M1-Macrophage infiltrate neuroblastoma
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422257.V1
Abstract: Supplementary Figure 4 Macrophage infiltration and downstream effects on neuroblastoma tumours
Publisher: Elsevier BV
Date: 10-2021
DOI: 10.1016/J.TVJL.2021.105731
Abstract: Streptococcus uberis (S. uberis) is a mastitis pathogen with an environmental reservoir. Management factors related to housing design and bedding are associated with the risk of S. uberis mastitis. This study aimed to investigate the ability of five distinct strains of S. uberis to survive and replicate on three common bedding materials (sand, wheat straw and kiln dried pine sawdust). Sterilized bedding substrates were inoculated with S. uberis and incubated at room temperature. Bacterial recovery from these media over time indicated that S. uberis numbers increased on used bedding materials, suggesting the addition of faeces and urine promoted replication. The bacterium was recovered for at least 35 days on straw and sand bedding, but could not be recovered beyond 7 days on clean or used sawdust. This study demonstrates the importance of bedding type and management on the environmental survival of S. uberis.
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422248
Abstract: Supplementary Materials and Methods CLEAN version
Publisher: American Dairy Science Association
Date: 11-2021
Publisher: American Society of Hematology
Date: 09-04-2015
DOI: 10.1182/BLOOD-2014-09-600643
Abstract: Arginase depletion with BCT-100 pegylated recombinant human arginase is cytotoxic to AML blasts.
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422263.V1
Abstract: Supplementary Figure 2 Cytokine profile of tumour induced macrophages or granulocytes
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422263
Abstract: Supplementary Figure 2 Cytokine profile of tumour induced macrophages or granulocytes
Publisher: Microbiology Society
Date: 06-2012
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422260
Abstract: Supplementary Figure 3 Neuroblastoma conditioning upregulates IL-1ï�¢ï€ and TNF-ï�¡ï€ expression in macrophages
Publisher: Springer Science and Business Media LLC
Date: 28-01-2009
Abstract: Streptococcus uberis , a Gram positive bacterial pathogen responsible for a significant proportion of bovine mastitis in commercial dairy herds, colonises multiple body sites of the cow including the gut, genital tract and mammary gland. Comparative analysis of the complete genome sequence of S. uberis strain 0140J was undertaken to help elucidate the biology of this effective bovine pathogen. The genome revealed 1,825 predicted coding sequences (CDSs) of which 62 were identified as pseudogenes or gene fragments. Comparisons with related pyogenic streptococci identified a conserved core (40%) of orthologous CDSs. Intriguingly, S. uberis 0140J displayed a lower number of mobile genetic elements when compared with other pyogenic streptococci, however bacteriophage-derived islands and a putative genomic island were identified. Comparative genomics analysis revealed most similarity to the genomes of Streptococcus agalactiae and Streptococcus equi subsp. zooepidemicus . In contrast, streptococcal orthologs were not identified for 11% of the CDSs, indicating either unique retention of ancestral sequence, or acquisition of sequence from alternative sources. Functions including transport, catabolism, regulation and CDSs encoding cell envelope proteins were over-represented in this unique gene set a limited array of putative virulence CDSs were identified. S. uberis utilises nutritional flexibility derived from a ersity of metabolic options to successfully occupy a discrete ecological niche. The features observed in S. uberis are strongly suggestive of an opportunistic pathogen adapted to challenging and changing environmental parameters.
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422266.V1
Abstract: Supplementary Figure 1: M1-Macrophage infiltrate neuroblastoma
Publisher: Elsevier BV
Date: 02-2017
DOI: 10.1016/J.BBAMEM.2016.12.002
Abstract: The anionic-polyelectrolyte nature of the wall of Gram-positive bacteria has long been suspected to be involved in homeostasis of essential cations and bacterial growth. A better understanding of the coupling between the biophysics and the biology of the wall is essential to understand some key features at play in ion-homeostasis in this living system. We consider the wall as a polyelectrolyte gel and balance the long-range electrostatic repulsion within this structure against the penalty entropy required to condense cations around wall polyelectrolytes. The resulting equations define how cations interact physically with the wall and the characteristic time required for a cation to leave the wall and enter into the bacterium to enable its usage for bacterial metabolism and growth. The model was challenged against experimental data regarding growth of Gram-positive bacteria in the presence of varying concentration of alent ions. The model explains qualitatively and quantitatively how alent cations interact with the wall as well as how the biophysical properties of the wall impact on bacterial growth (in particular the initiation of bacterial growth). The interplay between polymer biophysics and the biology of Gram positive bacteria is defined for the first time as a new set of variables that contribute to the kinetics of bacterial growth. Providing an understanding of how bacteria capture essential metal cations in way that does not follow usual binding laws has implications when considering the control of such organisms and their ability to survive and grow in extreme environments.
Publisher: American Association for Cancer Research (AACR)
Date: 08-2015
DOI: 10.1158/0008-5472.CAN-14-3443
Abstract: Neuroblastoma is the most common extracranial solid tumor of childhood, and survival remains poor for patients with advanced disease. Novel immune therapies are currently in development, but clinical outcomes have not matched preclinical results. Here, we describe key mechanisms in which neuroblastoma inhibits the immune response. We show that murine and human neuroblastoma tumor cells suppress T-cell proliferation through increased arginase activity. Arginase II is the predominant isoform expressed and creates an arginine-deplete local and systemic microenvironment. Neuroblastoma arginase activity results in inhibition of myeloid cell activation and suppression of bone marrow CD34+ progenitor proliferation. Finally, we demonstrate that the arginase activity of neuroblastoma impairs NY-ESO-1–specific T-cell receptor and GD2-specific chimeric antigen receptor–engineered T-cell proliferation and cytotoxicity. High arginase II expression correlates with poor survival for patients with neuroblastoma. The results support the hypothesis that neuroblastoma creates an arginase-dependent immunosuppressive microenvironment in both the tumor and blood that leads to impaired immunosurveillance and suboptimal efficacy of immunotherapeutic approaches. Cancer Res 75(15) 3043–53. ©2015 AACR.
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422254.V1
Abstract: Supplementary Figure 5 : IL-1ï�¢ï€ and TNF-ï�¡ï€ drive neuroblastoma cell proliferation
Publisher: Elsevier BV
Date: 09-2008
DOI: 10.1016/J.MIMET.2008.04.008
Abstract: Johne's disease is a slowly developing intestinal disease, primarily of ruminants, caused by Mycobacterium avium subspecies paratuberculosis. The disease contributes to significant economic losses worldwide in agricultural industry. Analysis of bacterial proteomes isolated directly from infected animals can provide important information about the repertoire of proteins present during infection and disease progression. In this study, M. avium subspecies paratuberculosis has been extracted from Johne's disease-infected cattle and goat intestinal tissue sections in a manner compatible with direct 2-DE proteomic analysis for comparison with in vitro-cultured bacteria. M. avium subspecies paratuberculosis was harvested from the submucosa and mucosa of intestinal sections and enriched from macerated tissue by hypotonic lysis, sonication and centrifugation through a viscosity gradient. Subsequent comparison of the proteomes of the in vivo- and in vitro-derived bacteria identified a number of proteins that were differentially expressed. Among them, a number of hypothetical proteins of unknown function and a hypothetical fatty acyl dehydrogenase (FadE3_2) and 3-hydroxyacyl-CoA dehydrogenase, possibly important for in vivo metabolism, utilising the pathway for the beta-oxidation of fatty acids.
Publisher: Springer Science and Business Media LLC
Date: 23-04-2015
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422260.V1
Abstract: Supplementary Figure 3 Neuroblastoma conditioning upregulates IL-1ï�¢ï€ and TNF-ï�¡ï€ expression in macrophages
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422254
Abstract: Supplementary Figure 5 : IL-1ï�¢ï€ and TNF-ï�¡ï€ drive neuroblastoma cell proliferation
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422257
Abstract: Supplementary Figure 4 Macrophage infiltration and downstream effects on neuroblastoma tumours
Publisher: Hindawi Limited
Date: 08-12-2020
DOI: 10.1155/2020/8828624
Abstract: Streptococcus uberis is one of the leading causes worldwide of mastitis in the dairy industry, with the most likely sources of infection attributed to environmental reservoirs such as contaminated bedding materials. Early detection of those cases most likely to progress to clinical disease would lead to improved animal welfare, a critical component of overall health and productivity. A multiplex PCR-based diagnostic test was developed for detection of S. uberis directly from milk and targeting two genes previously identified as important for intramammary colonisation and persistence in dairy cattle. Results indicated the threshold for detection directly from milk was 20,000 CFU/ml and this was achieved without the need for preenrichment. In addition, S. uberis could be identified from milk s les collected during intramammary challenge studies, prior to clinical signs of infection and at much lower detection limits. The PCR test developed for confirmation of the presence of S. uberis directly from infected milk has potential value as a diagnostic test to identify early infection and/or to confirm that antibiotic therapy has been successful.
Publisher: American Association for Cancer Research (AACR)
Date: 02-2019
DOI: 10.1158/0008-5472.CAN-18-2139
Abstract: These findings illustrate that cross-talk between myeloid cells and tumor cells creates a metabolic regulatory loop that promotes neuroblastoma progression.
Publisher: EDP Sciences
Date: 04-06-2010
Publisher: American Society for Microbiology
Date: 03-09-2020
DOI: 10.1128/MRA.00674-20
Abstract: Here, we report the complete genome of piscine Streptococcus agalactiae 01173 serotype Ia, which was generated using long-read sequencing technology. The bacteria were isolated from wild fish displaying signs of streptococcosis, from a fish kill incident in Kuwait.
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422251
Abstract: Supplementary Figure Legends and Tables
Publisher: Springer Science and Business Media LLC
Date: 18-05-2018
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422248.V1
Abstract: Supplementary Materials and Methods CLEAN version
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.22422251.V1
Abstract: Supplementary Figure Legends and Tables
Publisher: American Association for Cancer Research (AACR)
Date: 31-03-2023
DOI: 10.1158/0008-5472.C.6511169.V1
Abstract: Abstract Neuroblastoma is the most common childhood solid tumor, yet the prognosis for high-risk disease remains poor. We demonstrate here that arginase 2 (ARG2) drives neuroblastoma cell proliferation via regulation of arginine metabolism. Targeting arginine metabolism, either by blocking cationic amino acid transporter 1 (CAT-1)–dependent arginine uptake i in vitro /i or therapeutic depletion of arginine by pegylated recombinant arginase BCT-100, significantly delayed tumor development and prolonged murine survival. Tumor cells polarized infiltrating monocytes to an M1-macrophage phenotype, which released IL1β and TNFα in a RAC-alpha serine/threonine-protein kinase (AKT)–dependent manner. IL1β and TNFα established a feedback loop to upregulate ARG2 expression via p38 and extracellular regulated kinases 1/2 (ERK1/2) signaling in neuroblastoma and neural crest–derived cells. Proteomic analysis revealed that enrichment of IL1β and TNFα in stage IV human tumor microenvironments was associated with a worse prognosis. These data thus describe an immune-metabolic regulatory loop between tumor cells and infiltrating myeloid cells regulating ARG2, which can be clinically exploited. Significance: These findings illustrate that cross-talk between myeloid cells and tumor cells creates a metabolic regulatory loop that promotes neuroblastoma progression. /
Publisher: American Dairy Science Association
Date: 05-2020
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Sharon Egan.