ORCID Profile
0000-0003-2828-4829
Current Organisations
Directorate of Maize Research
,
University of Western Australia
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Publisher: Computers, Materials and Continua (Tech Science Press)
Date: 2022
Publisher: Sciencedomain International
Date: 19-02-2020
DOI: 10.9734/CJAST/2020/V39I130487
Abstract: A set of 48 rice genotypes were evaluated to assess the magnitude of genetic ersity under irrigated (control) and reproductive stage drought stress conditions during Kharif, 2018 at Rice Research Farm, Bihar Agricultural University, Sabour (Bhagalpur), India. On the basis of D2 statistics, all the genotypes were grouped into nineteen clusters in irrigated condition with cluster I consisting of maximum number of genotypes (24) followed by cluster III (7) and rest of the clusters were represented by single genotype in irrigated condition. Under drought stress condition, forty eight genotypes were grouped into eleven clusters, cluster I consisted of maximum number of genotypes (24) followed by cluster II and III (8 genotypes in each cluster) and rest of the clusters were mono-genotypic. The highest inter-cluster distance was recorded between cluster XVIII and XIX (28.53), followed by cluster X and VIII (24.20), cluster XIII and XVIII (23.98) and cluster VII and XVIII (23.79) in irrigated condition while in drought stress condition the highest inter-cluster distance was observed between cluster IX and X (31.72), followed by cluster V and IX (28.77), cluster VI and VII (25.98) and cluster IV and IX (25.98) indicating wider genetic ersity among the genotypes between these clusters. The hybridisation programme involving genotype of cluster XVIII and cluster XIX under irrigated condition and the genotypes of cluster IX and X under drought stress condition could be undertaken to isolate high yielding segregants, since these genotypes have high yielding potential, number of effective tillers per hill, relative water content, leaf area, root biomass, panicle length, biological yield, harvest index, plant height, number of fertile grains per panicle, total number of spikelets per panicle, leaf area and proline content with more genetic distances. The parents for hybridisation could be selected on the basis of their large inter-cluster distance for isolating useful recombinants in the segregating generations. Hence, these genotypes might be used in a multiple crossing programme to recover transgressive segregants. Therefore, it is suggested that if the erse genotypes from these groups along with the other desirable attributes are used in breeding programmes, it is expected to produce better segregants for high grain yield and yield contributing traits due to non-allelic interaction.
Publisher: Springer Science and Business Media LLC
Date: 25-06-2022
DOI: 10.1007/S11033-022-07679-5
Abstract: Conventional agricultural practices rely heavily on chemical fertilizers to boost production. Among the fertilizers, phosphatic fertilizers are copiously used to ameliorate low-phosphate availability in the soil. However, phosphorus-use efficiency (PUE) for major cereals, including maize, is less than 30% resulting in more than half of the applied phosphate being lost to the environment. Rock phosphate reserves are finite and predicted to exhaust in near future with the current rate of consumption. Thus, the dependence of modern agriculture on phosphatic fertilizers poses major food security and sustainability challenges. Strategies to optimize and improve PUE, like genetic interventions to develop high PUE cultivars, could have a major impact in this area. Here, we present the current understanding and recent advances in the biological phenomenon of phosphate uptake, translocation, and adaptive responses of plants under phosphate deficiency, with special reference to maize. Maize is one of the most important cereal crops that is cultivated globally under erse agro-climatic conditions. It is an industrial, feed and food crop with multifarious uses and a fast-rising global demand and consumption. The interesting aspects of ersity in the root system architecture traits, the interplay between signaling pathways contributing to PUE, and an in-depth discussion on promising candidate genes for improving PUE in maize are elaborated.
Publisher: Springer Science and Business Media LLC
Date: 15-06-2022
Publisher: MDPI AG
Date: 17-03-2022
Abstract: Several maize breeding programs in India have developed numerous inbred lines but the lines have not been characterized using high-density molecular markers. Here, we studied the molecular ersity, population structure, and linkage disequilibrium (LD) patterns in a panel of 314 tropical normal corn, two sweet corn, and six popcorn inbred lines developed by 17 research centers in India, and 62 normal corn from the International Maize and Wheat Improvement Center (CIMMYT). The 384 inbred lines were genotyped with 60,227 polymorphic single nucleotide polymorphisms (SNPs). Most of the pair-wise relative kinship coefficients (58.5%) were equal or close to 0, which suggests the lack of redundancy in the genomic composition in the majority of inbred lines. Genetic distance among most pairs of lines (98.3%) varied from 0.20 to 0.34 as compared with just 1.7% of the pairs of lines that differed by .20, which suggests greater genetic variation even among sister lines. The overall average of 17% heterogeneity was observed in the panel indicated the need for further inbreeding in the high heterogeneous genotypes. The mean nucleotide ersity and frequency of polymorphic sites observed in the panel were 0.28 and 0.02, respectively. The model-based population structure, principal component analysis, and phylogenetic analysis revealed three to six groups with no clear patterns of clustering by centers-wise breeding lines, types of corn, kernel characteristics, maturity, plant height, and ear placement. However, genotypes were grouped partially based on their source germplasm from where they derived.
Publisher: Public Library of Science (PLoS)
Date: 08-12-2014
Publisher: Springer Science and Business Media LLC
Date: 12-2021
Publisher: Springer Science and Business Media LLC
Date: 10-03-2022
DOI: 10.1007/S00122-022-04060-9
Abstract: Improving crop resistance against insect pests is crucial for ensuring future food security. Integrating genomics with modern breeding methods holds enormous potential in dissecting the genetic architecture of this complex trait and accelerating crop improvement. Insect resistance in crops has been a major research objective in several crop improvement programs. However, the use of conventional breeding methods to develop high-yielding cultivars with sustainable and durable insect pest resistance has been largely unsuccessful. The use of molecular markers for identification and deployment of insect resistance quantitative trait loci (QTLs) can fastrack traditional breeding methods. Till date, several QTLs for insect pest resistance have been identified in field-grown crops, and a few of them have been cloned by positional cloning approaches. Genome editing technologies, such as CRISPR/Cas9, are paving the way to tailor insect pest resistance loci for designing crops for the future. Here, we provide an overview of erse defense mechanisms exerted by plants in response to insect pest attack, and review recent advances in genomics research and genetic improvements for insect pest resistance in major field crops. Finally, we discuss the scope for genomic breeding strategies to develop more durable insect pest resistant crops.
Publisher: The Indian Society of Genetics and Plant Breeding
Date: 25-04-2022
Abstract: The present study involved estimating the combining ability effects and heterotic grouping to increase the efficacy of parental selection for successful hybrid development. A total of 52 F1 hybrids were generated by Line × Tester mating design by crossing 26 erse maize inbred lines with two testers, namely, HKI 323 and HKI 1105. The 26 baby corn inbred lines were classified into a different heterotic group based on the specific combining ability (SCA) effects and heterotic groups specific and general combining ability (HSGCA). The SCA effects and HSGCA of baby corn yield without husk (BCY) classified the inbred lines into five and four groups, respectively. This is the first study of heterotic’s grouping for baby corn germplasm considering BCY based on SCA and HSGCA methods. The information generated through heterotic grouping may be used to derive new inbred lines and develop different heterotic combinations for further utilization in breeding programs.
Publisher: Puspa Publishing House
Date: 31-08-2021
Abstract: The present study was conducted on genetic ersity analyses among 24 pea genotypes during 2017–2018 to assess the molecular ersity of pea genotypes using SSR markers. Out of 62, eleven markers were found to be polymorphic and the polymorphic information content (PIC) of the simple sequence repeat (SSR) markers ranged from 0.19 to as high as 0.64. Molecular profiling of these genotypes using 11 SSRs distributed throughout the genome generated 32 alleles with a mean of 2.91 alleles per locus. The genetic dissimilarity based on simple matching coefficient for 24 genotypes ranged from 0.00 to 0.91 with an average of 0.52. Cluster analyses grouped 24 genotypes into two major clusters with one outlier and supported by principal coordinate analysis (PCoA) in which genotypes were distributed across four quadrangles. Analysis of molecular variance (AMOVA) showed significant estimated value at degree of 1000 permutations. Percentage of variability was higher among in idual (67%) than among populations (11%). Percentage of variability within in idual was also higher (22%) than among populations (11%). Pop1 (I=0.707, He=0.446, and uHe=0.466) shows higher ersity than pop2 (I=0.630, He=0.381 and uHe=0.398). The percentage of polymorphic loci per population (PPL) ranged from 81.82% (pop2) to 90.91% (pop1) with an average of 86.36%. The present study demonstrates the utility of microsatellite markers for estimating molecular ersity as well as genotype identification in pea. This study also suggests a potential use of these markers in further association studies.
Publisher: Diva Enterprises Private Limited
Date: 2017
Publisher: The Indian Society of Genetics and Plant Breeding
Date: 2014
Publisher: MDPI AG
Date: 04-03-2022
DOI: 10.3390/IJMS23052838
Abstract: Heat stress (HS) is one of the major abiotic stresses affecting the production and quality of wheat. Rising temperatures are particularly threatening to wheat production. A detailed overview of morpho-physio-biochemical responses of wheat to HS is critical to identify various tolerance mechanisms and their use in identifying strategies to safeguard wheat production under changing climates. The development of thermotolerant wheat cultivars using conventional or molecular breeding and transgenic approaches is promising. Over the last decade, different omics approaches have revolutionized the way plant breeders and biotechnologists investigate underlying stress tolerance mechanisms and cellular homeostasis. Therefore, developing genomics, transcriptomics, proteomics, and metabolomics data sets and a deeper understanding of HS tolerance mechanisms of different wheat cultivars are needed. The most reliable method to improve plant resilience to HS must include agronomic management strategies, such as the adoption of climate-smart cultivation practices and use of osmoprotectants and cultured soil microbes. However, looking at the complex nature of HS, the adoption of a holistic approach integrating outcomes of breeding, physiological, agronomical, and biotechnological options is required. Our review aims to provide insights concerning morpho-physiological and molecular impacts, tolerance mechanisms, and adaptation strategies of HS in wheat. This review will help scientific communities in the identification, development, and promotion of thermotolerant wheat cultivars and management strategies to minimize negative impacts of HS.
Publisher: Springer Science and Business Media LLC
Date: 31-03-2020
DOI: 10.1007/S00425-020-03372-8
Abstract: While transgenic technology has heralded a new era in crop improvement, several concerns have precluded their widespread acceptance. Alternative technologies, such as cisgenesis and genome-editing may address many of such issues and facilitate the development of genetically engineered crop varieties with multiple favourable traits. Genetic engineering and plant transformation have played a pivotal role in crop improvement via introducing beneficial foreign gene(s) or silencing the expression of endogenous gene(s) in crop plants. Genetically modified crops possess one or more useful traits, such as, herbicide tolerance, insect resistance, abiotic stress tolerance, disease resistance, and nutritional improvement. To date, nearly 525 different transgenic events in 32 crops have been approved for cultivation in different parts of the world. The adoption of transgenic technology has been shown to increase crop yields, reduce pesticide and insecticide use, reduce CO
Publisher: Elsevier
Date: 2019
Publisher: Springer Science and Business Media LLC
Date: 16-05-2019
DOI: 10.1007/S10142-019-00684-1
Abstract: The ever-rising population of the twenty-first century together with the prevailing challenges, such as deteriorating quality of arable land and water, has placed a big challenge for plant breeders to satisfy human needs for food under erratic weather patterns. Rice, wheat, and maize are the major staple crops consumed globally. Drought, waterlogging, heat, salinity, and mineral toxicity are the key abiotic stresses drastically affecting crop yield. Conventional plant breeding approaches towards abiotic stress tolerance have gained success to limited extent, due to the complex (multigenic) nature of these stresses. Progress in breeding climate-resilient crop plants has gained momentum in the last decade, due to improved understanding of the physiochemical and molecular basis of various stresses. A good number of genes have been characterized for adaptation to various stresses. In the era of novel molecular markers, mapping of QTLs has emerged as viable solution for breeding crops tolerant to abiotic stresses. Therefore, molecular breeding-based development and deployment of high-yielding climate-resilient crop cultivars together with climate-smart agricultural practices can pave the path to enhanced crop yields for smallholder farmers in areas vulnerable to the climate change. Advances in fine mapping and expression studies integrated with cheaper prices offer new avenues for the plant breeders engaged in climate-resilient plant breeding, and thereby, hope persists to ensure food security in the era of climate change.
Publisher: Springer Science and Business Media LLC
Date: 11-04-2023
Publisher: Elsevier BV
Date: 09-2022
Publisher: Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Date: 16-12-2020
DOI: 10.56093/IJAS.V90I11.108564
Abstract: The success of any pearl millet hybrid programme depends upon the assessment of the structure of parental ersity. In this investigation, polymorphism data generated using 42 simple sequence repeat (SSR) markers and 12 agromorphological traits were used for genetic ersity assessment among 32 genotypes (29 restorers and 3 maintainers) of pearl millet [Pennisetum glaucum (L.) R. Br.].In contrast to morphological data, SSR based clustering pattern and Principal Coordinate Analysis revealed that the genotypes were found to be consistent with their pedigree and origin. Polymorphism information content (PIC) values based on microsatellites ranged from 0.117 to 0.841 per locus with an average PIC of 0.348. Allelic richness varied from two to five per locus. The highest similarity index (0.74) was observed between restorers WGI 58 and WGI 148, whereas the lowest similarity index (0.27) was observed between seed parent 841 B and PPMI 269. AMOVA analysis observed more genetic variance among the in iduals than within in iduals.The study led to the identification of maintainer 841 B as a genetically erse parent from other maintainers as well as among most of the restorers and hence may be used in future hybrid development programmes. Further, the correlation between phenotypic and genetic distance matrices was observed to be very low. Appropriate heterotic combinations were identified based on morphological and molecular ersity.
Publisher: Elsevier
Date: 2021
Publisher: Springer International Publishing
Date: 2021
Publisher: Springer International Publishing
Date: 2023
Publisher: MDPI AG
Date: 03-02-2022
Abstract: Heat stress events during flowering in Brassica crops reduce grain yield and are expected to increase in frequency due to global climate change. We evaluated heat stress tolerance and molecular genetic ersity in a global collection of Brassica rapa accessions, including leafy, rooty and oilseed morphotypes with spring, winter and semi-winter flowering phenology. Tolerance to transient daily heat stress during the early reproductive stage was assessed on 142 lines in a controlled environment. Well-watered plants of each genotype were exposed to the control (25/15 °C day/night temperatures) or heat stress (35/25 °C) treatments for 7 d from the first open flower on the main stem. Bud and leaf temperature depression, leaf conductance and chlorophyll content index were recorded during the temperature treatments. A large genetic variation for heat tolerance and sensitivity was found for above-ground biomass, whole plant seed yield and harvest index and seed yield of five pods on the main stem at maturity. Genetic ersity was assessed on 212 lines with 1602 polymorphic SNP markers with a known location in the B. rapa physical map. Phylogenetic analyses confirmed two major genetic populations: one from East and South Asia and one from Europe. Heat stress-tolerant lines were distributed across erse geographic origins, morphotypes (leafy, rooty and oilseed) and flowering phenologies (spring, winter and semi-winter types). A genome-wide association analysis of heat stress-related yield traits revealed 57 SNPs distributed across all 10 B. rapa chromosomes, some of which were associated with potential candidate genes for heat stress tolerance.
Publisher: Research Square Platform LLC
Date: 23-05-2023
DOI: 10.21203/RS.3.RS-2952076/V1
Abstract: Maydis leaf blight (MLB) is a prevalent disease affecting maize worldwide, caused by the necrotrophic plant pathogen Bipolaris maydis (Nisikado and Miyake). Depending on environmental conditions, MLB can lead to yield losses of up to 40% or more. To combat this disease, various chemical and biocontrol/botanical agents have been developed and proven effective. This study aimed to assess the efficacy of different combinations of disease management components as an alternative approach. The effectiveness of three modules, namely organic, chemical, and IDM, was tested in hot spot locations, namely Ludhiana, Karnal, and Delhi, during 2019 and 2020. Results indicated that the chemical module demonstrated superior disease control, achieving percentages of 54.16 and 52.92 at Ludhiana and Karnal, respectively. The IDM module also showed promising results, with disease control percentages of 45.87 and 44.69 at Ludhiana and Karnal, respectively. Conversely, the organic module exhibited the least effectiveness. Notably, at the Delhi location, the standard control (Foliar spray with Mancozeb 75 WP @ 2.5 g / l water) proved most effective, achieving a disease control percentage of 64.29, followed by the IDM module at 50.00. The chemical module exhibited the highest percent increase in yield (PIY), with figures of 86.47 and 52.92 at Ludhiana and Karnal, respectively, compared to other treatments. This study highlights the superior efficacy of the chemical and IDM modules in comparison to the positive control (check). Consequently, these modules present alternative strategies for effectively managing MLB.
Publisher: Springer Science and Business Media LLC
Date: 05-03-2022
Publisher: Springer International Publishing
Date: 2018
Publisher: Excellent Publishers
Date: 10-04-2017
Publisher: Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Date: 29-03-2022
DOI: 10.56093/IJAS.V92I3.122539
Abstract: Maize (Zea mays L.) is the third most important staple crop after rice and wheat with enormous ersity and adaptation ability. Hybrid breeding is the most important approach for developing high yielding cultivars in maize. It relies upon the generation of pure inbred lines with desirable traits in quick span to achieve higher genetic gains. Rapidly rising global population and climate change necessitates the development of innovative technologies that can help to safeguard the food security in future. Doubled Haploid (DH) technology is the best approach for rapid development of new inbred lines and has contributed immensely in the rapid generation of inbred lines and hybrid development. In addition, the use of molecular markers with DH technology resulted into mapping of genomic regions for different traits. The recent development in identification of alternative markers for haploid selection and genome editing approaches will further strengthen the DH technology for commercial maize breeding. This review describes important landmarks of maize DH technology, its applications, and recent advances in utilization of emerging technologies, viz. CRIPSR-cas and genomics approaches for DH technology
Publisher: Research Square Platform LLC
Date: 08-2023
DOI: 10.21203/RS.3.RS-3198338/V1
Abstract: Viral diseases substantially impact global maize production. Genetic resistance is the most feasible approach to combat losses caused by viral diseases. Various researchers have mapped several QTLs for viral disease resistance (VDR) in maize. The meta-QTL (MQTL) analysis is the best way to leverage such independent studies to find overlapping or common genomic regions governing target traits. Towards this direction, in the present study, the MQTL analysis was carried out using a total of 39 QTL mapping investigations on resistance against 14 viral diseases. 53 (27.04%) out of 196 QTLs could be projected on the maize genome. The study discovered 14 MQTLs for resistance against 11 viral diseases on chromosomes 1, 3 and 10. Marker trait associations (MTAs) in genome-wide association studies confirmed the role of two MQTLs (MQTL3_2 and MQTL10_2) for VDR. The identified MQTL regions harboured 1715 candidate genes. Further, the genes expressed constitutively in the MQTLs identified for VDR were analyzed for their involvement in metabolic pathways. The identified genes were found to be implicated in antiviral resistance, and thus, constitute a good resource for allele mining to decipher the mechanism of plant-virus interactions.
Publisher: Acta Scientific Publications Pvt. Ltd.
Date: 09-2022
Publisher: Springer Science and Business Media LLC
Date: 16-08-2021
Publisher: Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Date: 02-12-2021
DOI: 10.56093/IJAS.V91I11.118535
Abstract: Pooled analysis of the test-crosses evaluation of 61 newly developed maize (Zea mays L.) inbred lines usingtwo inbred testers namely BML-6 and BML-7 during rabi 2016-17 and 2017-18 at ICAR-IIMR Regional Centre,Begusarai revealed significant variation among genotypes, general combining ability (GCA) and specific combiningability (SCA) effects for all the traits. Out of 61 inbred lines, 29 exhibited significant positive GCA effect for grainyield with maximum GCA effect value of 25.64. Inbred lines, viz. IMLSB-1299-5, IMLSB-406-2, IMLSB-334B-2,IMLSB-814-2 and IMLSB-285-1 were identified as the best general combiners on the basis of GCA effects whichcan be utilized to a greater extent in hybrid breeding programme. Out of 29 inbred lines with significant positive GCAeffects, 12 and 17 lines also showed significant positive SCA effects with tester, BML-7 and BML-6 respectively, thuswere classified into two heterotic groups ‘A’ and ‘B’, respectively. Out of 122 test-crosses, 14 exhibited significantand positive heterosis for yield over three national checks. The information generated on the heterotic grouping willhelp in further streamlining the available germplasm into heterotic pools and thereby augmenting the national hybridbreeding programme.
Publisher: Springer International Publishing
Date: 2023
Publisher: MDPI AG
Date: 27-03-2022
Abstract: Advances in sequencing technologies and bioinformatics tools have fueled a renewed interest in whole genome sequencing efforts in many organisms. The growing availability of multiple genome sequences has advanced our understanding of the within-species ersity, in the form of a pangenome. Pangenomics has opened new avenues for future research such as allowing dissection of complex molecular mechanisms and increased confidence in genome mapping. To comprehensively capture the genetic ersity for improving plant performance, the pangenome concept is further extended from species to genus level by the inclusion of wild species, constituting a super-pangenome. Characterization of pangenome has implications for both basic and applied research. The concept of pangenome has transformed the way biological questions are addressed. From understanding evolution and adaptation to elucidating host–pathogen interactions, finding novel genes or breeding targets to aid crop improvement to design effective vaccines for human prophylaxis, the increasing availability of the pangenome has revolutionized several aspects of biological research. The future availability of high-resolution pangenomes based on reference-level near-complete genome assemblies would greatly improve our ability to address complex biological problems.
Publisher: MDPI AG
Date: 23-11-2021
DOI: 10.3390/SU132312975
Abstract: Micronutrient deficiencies, particularly iron (Fe) and zinc (Zn), in human diets are affecting over three billion people globally, especially in developing nations where diet is cereal-based. Wheat is one of several important cereal crops that provide food calories to nearly one-third of the population of the world. However, the bioavailability of Zn and Fe in wheat is inherently low, especially under Zn deficient soils. Although various fortification approaches are available, biofortification, i.e., development of mineral-enriched cultivars, is an efficient and sustainable approach to alleviate malnutrition. There is enormous variability in Fe and Zn in wheat germplasm, especially in wild relatives, but this is not utilized to the full extent. Grain Fe and Zn are quantitatively inherited, but high-heritability and genetic correlation at multiple locations indicate the high stability of Fe and Zn in wheat. In the last decade, pre-breeding activities have explored the potential of wild relatives to develop Fe and Zn rich wheat varieties. Furthermore, recent advances in molecular biology have improved the understanding of the uptake, storage, and bioavailability of Fe and Zn. Various transportation proteins encoding genes like YSL 2, IRT 1, OsNAS 3, VIT 1, and VIT 2 have been identified for Fe and Zn uptake, transfer, and accumulation at different developing stages. Hence, the availability of major genomic regions for Fe and Zn content and genome editing technologies are likely to result in high-yielding Fe and Zn biofortified wheat varieties. This review covers the importance of wheat wild relatives for Fe and Zn biofortification, progress in genomics-assisted breeding, and transgenic breeding for improving Fe and Zn content in wheat.
Publisher: Springer Science and Business Media LLC
Date: 04-10-2023
Publisher: Public Library of Science (PLoS)
Date: 19-08-2021
DOI: 10.1371/JOURNAL.PONE.0256389
Abstract: The rising demand for popcorn necessitates improving the popping quality with higher yield of popcorn cultivars. Towards this direction several Quantitative Traits Loci (QTLs) for popping traits have been identified. However, identification of accurate and consistent QTLs across different genetic backgrounds and environments is necessary to effectively utilize the identified QTLs in marker-assisted breeding. In the current study, 99 QTLs related to popping traits reported in 8 different studies were assembled and projected on the reference map "Genetic 2005" using BioMercator v4.2 to identify metaQTLs with consistent QTLs. Total ten metaQTLs were identified on chromosome 1 (7 metaQTLs) and 6 (3 metaQTLs) with physical distance ranging between 0.43 and 12.75 Mb, respectively. Four identified metaQTLs, viz ., mQTL1_1, mQTL1_5, mQTL1_7 and mQTL6_2 harboured 5–8 QTL clusters with moderately high R 2 value. The clustered QTLs were from two or more experiments. Based on the expression pattern in endosperm and pericarp tissues, a total of 229 genes were selected. Nineteen of these genes are involved in carbohydrate metabolism. Of the 19 genes specifically involved in carbohydrate metabolism, 11 of them were in these regions, implying the importance of these clustered QTLs. MetaQTL1_1 at bin location 1.01 coincided with the reported QTLs related to various agronomic traits like stalk diameter, tassel length, leaf area and plant height. The identified metaQTLs can be further explored for fine mapping and candidate gene identification, which can be validated by loss or gain of function. Identified metaQTLs can be used for introgression of popping traits towards enhancing the popping ability.
Publisher: CABI
Date: 02-07-2021
Publisher: No publisher found
Date: 2022
Publisher: The Indian Society of Genetics and Plant Breeding
Date: 11-01-2020
Abstract: Baby corn has emerged as one of the most important sources to augment the farmer’s income in peri-urban areas. It has erse uses as vegetables, snacks, value-added products and assured supply of green fodder for livestock. The multilocation varietal trials mainly emphasize on the identification of new superior cultivars over commercial checks, while genotype×environment interaction (GEI) is ignored. In the current study, 13 baby corn hybrids were evaluated for green ear yield, baby corn yield and green fodder yield over eight locations (environments) in kharif seasons of 2015 and 2016 using GGE biplot analysis. The results revealed a higher proportion of the variation in the data is attributable to the environment (72.4-87.0%), while genotype contributed only 2.5-7.3% of the total variation. GEI contributed 10.5-24.1% of the total variation. Superior stable hybrids for green ear yield, baby corn yield and green fodder yield could be identified using a biplot graphical approach effectively. ‘Which won where’ plot for each of the traits partitioned testing locations into three mega-environments with different winning genotypes for different traits in respective mega-environments. Thus it can be concluded that similar inferences can be drawn from one or two representatives of each mega-environment instead of using several locations. Hence, the presence of extensive crossover GEI in baby corn multi-location trials clearly suggests the need to emphasize on smaller zonation of testing locations and location-specific breeding. Particularly in baby corn, this is the first study on GGE biplot analysis to identify mega-environments for effective evaluation of baby corn trials.
Publisher: The Indian Society of Genetics and Plant Breeding
Date: 2015
Publisher: Agricultural Research Communication Center
Date: 03-05-2022
DOI: 10.18805/IJAR.B-4771
Abstract: Background: This study was taken up to assess the effect of time of sowing and number of irrigations on the yield of maize cultivar J-1006 and to see their impact on the nutritive value of fresh and ensiled fodder. Methods: Maize cultivar J-1006 was sown at 50 cm row to row and 10 cm plant to plant spacing at 10 days interval in quintuplicate. The crop was harvested after 80-85 days of sowing. The J-1006 on the first date of sowing (B1) was given one irrigation and rest of the sowings (B2-B5) received two irrigations till harvest. The nutritive value (NV) of fresh and ensiled maize fodder was assessed by in vitro gas production (IVGP) technique. Result: Highest biological, DM and protein yield was recorded in B2 maize fodder while the lowest was recorded in B5. The cell wall constituents were the highest (P less than 0.01) in B1 and the lowest in B5 fodder resulting in highest (P less than 0.01) microbial biomass production (MBP) and the NV in B5 maize fodder. Irrespective of time of sowing, OM, NDF and hemicellulose contents were reduced (P less than 0.01) in ensiled maize fodder. The net gas production (NGP P less than 0.01) and ME (P less than 0.05) were depressed and the total and in idual VFAs and MBP were improved (P less than 0.01) in the ensiled maize. Irrespective of processing, the maize given two irrigations resulted in higher (P less than 0.01) MBP and NV as comparison to that given single irrigation. It was concluded that B5 maize fodder with the lowest yield given two irrigations had the best nutritive value.
Publisher: Elsevier BV
Date: 04-2023
Publisher: Elsevier
Date: 2023
Publisher: EJPB, ISPB, CPBG - Tamil Nadu Agricultural University
Date: 2016
Publisher: MDPI AG
Date: 22-05-2022
DOI: 10.3390/PROTEOMES10020017
Abstract: Wheat is an important staple cereal for global food security. However, climate change is h ering wheat production due to abiotic stresses, such as heat, salinity, and drought. Besides shoot architectural traits, improving root system architecture (RSA) traits have the potential to improve yields under normal and stressed environments. RSA growth and development and other stress responses involve the expression of proteins encoded by the trait controlling gene/genes. Hence, mining the key proteins associated with abiotic stress responses and RSA is important for improving sustainable yields in wheat. Proteomic studies in wheat started in the early 21st century using the two-dimensional (2-DE) gel technique and have extensively improved over time with advancements in mass spectrometry. The availability of the wheat reference genome has allowed the exploration of proteomics to identify differentially expressed or abundant proteins (DEPs or DAPs) for abiotic stress tolerance and RSA improvement. Proteomics contributed significantly to identifying key proteins imparting abiotic stress tolerance, primarily related to photosynthesis, protein synthesis, carbon metabolism, redox homeostasis, defense response, energy metabolism and signal transduction. However, the use of proteomics to improve RSA traits in wheat is in its infancy. Proteins related to cell wall biogenesis, carbohydrate metabolism, brassinosteroid biosynthesis, and transportation are involved in the growth and development of several RSA traits. This review covers advances in quantification techniques of proteomics, progress in identifying DEPs and/or DAPs for heat, salinity, and drought stresses, and RSA traits, and the limitations and future directions for harnessing proteomics in wheat improvement.
Publisher: Springer Science and Business Media LLC
Date: 28-06-2023
Publisher: Springer Science and Business Media LLC
Date: 02-2022
DOI: 10.1007/S12298-022-01146-Y
Abstract: In agro-ecosystem, plant pathogens h er food quality, crop yield, and global food security. Manipulation of naturally occurring defense mechanisms in host plants is an effective and sustainable approach for plant disease management. Various natural compounds, ranging from cell wall components to metabolic enzymes have been reported to protect plants from infection by pathogens and hence provide specific resistance to hosts against pathogens, termed as induced resistance. It involves various biochemical components, that play an important role in molecular and cellular signaling events occurring either before (elicitation) or after pathogen infection. The induction of reactive oxygen species, activation of defensive machinery of plants comprising of enzymatic and non-enzymatic antioxidative components, secondary metabolites, pathogenesis-related protein expression (e.g. chitinases and glucanases), phytoalexin production, modification in cell wall composition, melatonin production, carotenoids accumulation, and altered activity of polyamines are major induced changes in host plants during pathogen infection. Hence, the altered concentration of biochemical components in host plants restricts disease development. Such biochemical or metabolic markers can be harnessed for the development of “pathogen-proof” plants. Effective utilization of the key metabolites-based metabolic markers can pave the path for candidate gene identification. This present review discusses the valuable information for understanding the biochemical response mechanism of plants to cope with pathogens and genomics-metabolomics-based sustainable development of pathogen proof cultivars along with knowledge gaps and future perspectives to enhance sustainable agricultural production.
Publisher: Wiley
Date: 03-2023
DOI: 10.1111/JAC.12637
Abstract: Terminal heat stress is a major constraint for taking a profitable crop of wheat by small and marginal farmers in the Indo‐Gangetic Plains of south Asia. Hence, breeders remain in constant search for heat‐tolerant genotypes. This study was done with the purpose to find out high‐yielding wheat genotypes that perform stably under terminal heat stress as well as to conclude an easy phenotyping trait for this objective. A cross (HUW 234 × HUW 468) was made using two popular cultivars of the eastern Gangetic Plains of India. HUW 234 carries terminal heat tolerance whereas, HUW 468 is high yielding but suffers from terminal heat stress when planted late. So obtained 167 recombinant inbred lines (RILs) were exposed to both timely sown (TS) and late sown (LS) conditions at three locations for two consecutive years 2016–17 and 2017–18 at Varanasi (Uttar Pradesh), Jabalpur (Madhya Pradesh) and Pusa, Samastipur (Bihar). Combined ANOVA revealed that normalized difference vegetation index (NDVI) at anthesis stage (NDVI_AH) and grain filling duration (GFD) was significant for genotype (G), environment (E) and genotype × environment interaction (GEI). Likewise, differences were significant for grain yield (GY) and 1000‐grain weight (TGW). AMMI analysis revealed similar results. However, NDVI_AH showed low variation under heat stressed late sown conditions. Furthermore, the which‐won‐where model and mean versus stability identified the best performing, above‐average yielding and stable lines across the environments. Correlation among measured traits revealed that NDVI_AH had a significant association with GY in most of the environments. Several lines that performed superior to better parent for GY also showed higher values for NDVI_AH and GFD. The study demonstrates that NDVI_AH and GFD appear to be important traits to be considered while breeding for terminal heat stress tolerance in wheat.
Publisher: Society for Advancement of Wheat and Barley Research
Date: 05-05-2020
Publisher: Research Square Platform LLC
Date: 27-03-2023
DOI: 10.21203/RS.3.RS-2718476/V1
Abstract: Background : Maize is an excellent fodder crop due to its high biomass, better palatability, succulency, and nutrition. Studies on morpho-physiological and biochemical characterization of fodder maize are limited. The present study aimed to explore the genetic variation in fodder maize landraces for various morpho-physiological traits and estimation of genetic relationship and population structure. Methods and Results : The study in 47 fodder maize landraces revealed significant variation for all morpho-physiological traits except leaf-stem ratio. Plant height, stem girth, leaf-width and number of leaves showed positive correlation with green fodder yield. Morpho-physiological traits-based clustering grouped the landraces into three major clusters, whereas neighbour joining cluster and population structure analysis using 40 SSR markers revealed four and five major groups, respectively. Most landraces of Northern Himalaya-Kashmir and Ludhiana fall into a single group, whereas rest groups mainly had landraces from North-Eastern Himalaya. A total of 101alleles were generated with mean polymorphic information content value of 0.36 and major allele frequency of 0.68. The pair wise genetic dissimilarity between genotypes ranged from 0.21 and 0.67. Mantel test revealed weak but significant correlation between morphological and molecular distance. Biochemical characterisation of superior landraces revealed significant variation for neutral detergent fiber, acid detergent fiber, cellulose and lignin content. Conclusion : Interestingly, significant, and positive correlation of SPAD with lignin content can be explored to bypass the costly affair of invitro quality assessment for digestibility parameters. The study identified superior landraces and demonstrated the use of molecular markers in genetic ersity assessment and grouping of genotypes for fodder maize improvement.
Publisher: Informa UK Limited
Date: 04-05-2021
Publisher: Springer Science and Business Media LLC
Date: 02-06-2021
Publisher: Springer International Publishing
Date: 2023
Publisher: Wiley
Date: 25-02-2021
DOI: 10.1111/PPL.13343
Publisher: MDPI AG
Date: 29-03-2022
Abstract: Maize is an important cereal crop in the world for feed, food, fodder, and raw materials of industries. Turcicum leaf blight (TLB) is a major foliar disease that can cause more than 50% yield losses in maize. Considering this, the molecular ersity, population structure, and genome-wide association study (GWAS) for TLB resistance were studied in 288 erse inbred lines genotyped using 89 polymorphic simple sequence repeats (SSR) markers. These lines werescreened for TLB disease at two hot-spot locations under artificially inoculated conditions. The average percent disease incidence (PDI) calculated for each genotype ranged from 17 (UMI 1201) to 78% (IML 12-22) with an overall mean of 40%. The numbers of alleles detected at a locus ranged from twoto nine, with a total of 388 alleles. The polymorphic information content (PIC) of each marker ranged between 0.04 and 0.86. Out of 89 markers, 47 markers were highly polymorphic (PIC ≥ 0.60). This indicated that the SSR markers used were very informative and suitable for genetic ersity, population structure, and marker-trait association studies.The overall observed homozygosity for highly polymorphic markers was 0.98, which indicated that lines used were genetically pure. Neighbor-joining clustering, factorial analysis, and population structure studies clustered the 288 lines into 3–5 groups. The patterns of grouping were in agreement with the origin and pedigree records of the genotypesto a greater extent.A total of 94.10% lines were successfully assigned to one or another group at a membership probability of ≥0.60. An analysis of molecular variance (AMOVA) revealed highly significant differences among populations and within in iduals. Linkage disequilibrium for r2 and D′ between loci ranged from 0 to 0.77 and 0 to 1, respectively. A marker trait association analysis carried out using a general linear model (GLM) and mixed linear model (MLM), identified 15 SSRs markers significantly associated with TLB resistance.These 15 markers were located on almost all chromosomes (Chr) except 7, 8, and 9. The phenotypic variation explained by these loci ranged from 6% (umc1367) to 26% (nc130, phi085). Maximum 7 associated markers were located together on Chr 2 and 5. The selected regions identified on Chr 2 and 5 corroborated the previous studies carried out in the Indian maize germplasm. Further, 11 candidate genes were identified to be associated with significant markers. The identified sources for TLB resistance and associated markers may be utilized in molecular breeding for the development of suitable genotypes.
Publisher: MDPI AG
Date: 20-04-2022
Abstract: Meiosis is the least explored stage for thermotolerance in wheat. We evaluated the impact of 5 d of moderate transient daily heat stress during meiosis in the main stem spike on physiological and grain yield traits in 30 erse wheat cultivars which vary widely in heat tolerance and sensitivity. We found that a moderate heat stress event during meiosis in the main stem spike had lasting impacts on plant growth and reproduction in heat-sensitive, but not heat-tolerant, wheat cultivars. Heat-tolerant cultivars maintained grain yield, grain number and in idual grain weight in the main stem spike and also total plant grain yield and biomass in the heat stress treatment relative to the control. Heat-sensitive cultivars responded to heat stress by producing fewer and smaller grains per spikelet on the main stem, fewer tillers, lower biomass and lower total plant grain yield in the high temperature treatment relative to the control. Heat-sensitive cultivars produced higher flag leaf chlorophyll content in the high temperature treatment relative to the control than heat-tolerant cultivars during the first 3 d of heat treatment. There was small reduction in pollen viability from 98% to 96% following heat stress during meiosis which was unrelated to heat tolerance or sensitivity. Moderate transient heat stress during meiosis did not greatly reduce the production of viable male gametes, but had long-lasting negative impacts on fertilization and subsequent seed production in heat-sensitive cultivars.
Publisher: Springer Science and Business Media LLC
Date: 09-02-2015
Publisher: Springer Science and Business Media LLC
Date: 07-2023
Publisher: Wiley
Date: 21-11-2019
DOI: 10.1002/BBB.2060
No related grants have been discovered for Mukesh Choudhary.