Publication
Intraperitoneal transfer of wild‐type bone marrow repopulates tissue macrophages in the Csf1r knockout rat without contributing to monocytopoiesis
Publisher:
Wiley
Date:
05-2023
DOI:
10.1002/EJI.202250312
Abstract: Homozygous null mutation of the Csf1r gene ( Csf1rko ) in rats leads to the loss of most tissue macrophage populations and pleiotropic impacts on postnatal growth and organ maturation, leading to early mortality. The phenotype can be reversed by intraperitoneal transfer of WT BM cells (BMT) at weaning. Here, we used a Csf1r ‐mApple transgenic reporter to track the fate of donor‐derived cells. Following BMT into Csf1rko recipients, mApple +ve cells restored IBA1 + tissue macrophage populations in every tissue. However, monocytes, neutrophils, and B cells in the BM, blood, and lymphoid tissues remained of recipient (mApple −ve ) origin. An mApple +ve cell population expanded in the peritoneal cavity and invaded locally in the mesentery, fat pads, omentum, and diaphragm. One week after BMT, distal organs contained foci of mApple +ve , IBA1 −ve immature progenitors that appeared to proliferate, migrate, and differentiate locally. We conclude that rat BM contains progenitor cells that are able to restore, replace, and maintain all tissue macrophage populations in a Csf1rko rat directly without contributing to the BM progenitor or blood monocyte populations.