ORCID Profile
0000-0001-5499-060X
Current Organisations
Children's Hospital at Westmead
,
University of Technology Sydney
,
Western Sydney University
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Publisher: Elsevier BV
Date: 02-2017
Publisher: Informa UK Limited
Date: 12-2003
DOI: 10.1080/10520290410001671335
Abstract: Current uses of orcein to demonstrate elastic fibers and, following permanganate oxidation (Shikata's modification), hepatitis B surface antigen, copper associated protein, and sulfated mucins, are reviewed. Variations in staining performance with batch of dye and age of dye solution is also discussed. Additional experimental findings support the view that the orcein stain for elastic tissue and Shikata's modification produces consistent, high quality results as long as appropriate controls and suitable dye batches, e.g., Biological Stain Commission certified dyes, are used.
Publisher: Informa UK Limited
Date: 31-07-2018
Publisher: S. Karger AG
Date: 2007
DOI: 10.1159/000325762
Abstract: Urinary cytology is an uncommon investigative tool for pediatric tumors. A 16-month-old infant presented for investigation ofhematuria and blood clots into diapers. Urinary cytology showed a population of small, round, blue cells with little cytoplasm and high nuclear/cytoplasmic ratios. The cells were positive for desmin and negative for cytokeratin. A cytologic diagnosis of embryonal rhabdomyosarcoma was offered and subsequently confirmed by histopathology. Embryonal rhabdomyosarcomas are not uncommon in the pediatric population. Urinary cytology may be useful for rapid diagnosis and early management of such cases.
Publisher: Elsevier BV
Date: 06-1992
DOI: 10.1016/0165-1838(92)90053-J
Abstract: The occurrence, distribution and regional variation of neurones immunoreactive for the neuropeptides, vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), enkephalin (ENK), calcitonin gene-related peptide (CGRP), and substance P (SP) were investigated in human ureters by indirect immunohistochemistry. In addition, immunoreactivities to tyrosine hydroxylase (TH), a marker of noradrenergic neurones and to protein gene product (PGP) 9.5, a general marker of neurones, were also studied. Neurones displaying PGP-, NPY-, VIP- and TH-like immunoreactivity (-LIR) provided a rich innervation to the smooth muscle and blood vessels of the ureter, where they formed dense muscular and perivascular nerve plexuses. In contrast, there was only a moderate to sparse innervation by SP and CGRP-LIR neurones, most of which were distributed to blood vessels and to the sub mucosal layer, and only rarely to smooth muscle bundles. No ENK-LIR was detected in this study. Nerve fibre bundle densities were estimated for each of the localized neurochemicals according to a method described. NPY-LIR nerve fibre bundles were found to account for 80% of the total nerve fibre bundles (i.e. PGP-LIR) in the ureter. On the other hand, TH-LIR and VIP-LIR nerve fibre bundles each accounted for 50% of the total ureteral innervation, whereas SP- and CGRP-LIR nerve fibre bundles each comprised 20% of the total innervation. The abundance and pattern of tissues innervated by these immunoreactive neurones is consistent with the view that some of these neuropeptide substances co-exist with other peptide substances and/or with other known neurotransmitters, such as noradrenaline or acetylcholine. A gradient of innervation was found to exist for all the neurochemicals demonstrated in the ureter, whereby the lower ureter receives a greater density of innervation than the upper ureter. This finding suggests the human ureter is primarily innervated by fibres arising from or via the lower pelvis, i.e. the pelvic plexus. It also supports the view that the lower ureter may perform an important physiological role, such as coordinating the tone of this region during bladder filling and emptying.
Publisher: Informa UK Limited
Date: 2007
Publisher: Elsevier BV
Date: 07-2015
DOI: 10.1016/J.JPEDSURG.2014.10.046
Abstract: The Bethesda classification for cytology is used to classify thyroid nodules into one of six categories, and for each category there is an implied cancer risk and also recommendation for management. Despite lack of data in children, the American thyroid association promotes the use of the same management guidelines as in adults. Our aim was to study the risk of malignancy for each Bethesda class in children with thyroid nodules. We included all patients ≤18years of age that had underwent a thyroid fine needle aspiration (FNA) at one of two centers between January 1998 and July 2013. FNA results were reclassified according to the Bethesda criteria. Histological, repeat cytological, radiological and clinical follow-up were recorded. Fifty-six patients (66 nodules) underwent FNB. Mean age was 13.6 years. Numbers of nodules reported as BI-BVI were 7, 38, 11, 4, 3 and 3, respectively. Follow-up was achieved for 55 (83%) nodules. Twelve (18%) nodules were malignant by histology and revealed papillary (n=7), follicular (n=3) or insular thyroid cancer (n=2), The proportion of nodules with malignancy for BI-BVI was: 0%, 0%, 18%, 100%, 100% and 100%. The rate of malignancy in thyroid nodules in children seems to be higher than reported in adults. The Bethesda criteria seem to accurately identify benign nodules, but other categories have a very high rate of malignancy and BIII nodules pose a particular challenge.
Publisher: Informa UK Limited
Date: 21-09-2010
DOI: 10.3109/10520295.2010.518891
Abstract: Hot commercial dishwashing detergent has been used to deparaffinize and hydrate formalin fixed, paraffin embedded sections for immunohistochemistry. Fifty-five antibodies, used routinely for diagnosis, were used to compare hot detergent dewaxing with the proprietary hydrocarbon-based dewaxing reagent supplied with the Bond Max immunohistochemistry system®. A 2% concentration of commercial dishwashing detergent in distilled water was heated to 90° C and paraffin sections were treated twice for 1 min each. Nearly all antibodies gave equivalent results except CD10 and CD57 (hydrocarbon-based dewaxing better) and CD45 and alpha fetoprotein (detergent dewaxing better) the differences, however, were minimal. There also was a significant cost saving using detergent dewaxing.
Publisher: Wiley
Date: 02-1998
Publisher: SAGE Publications
Date: 25-11-2022
Publisher: Informa UK Limited
Date: 06-2013
Publisher: Informa UK Limited
Date: 03-2020
Publisher: Informa UK Limited
Date: 02-01-2017
Publisher: Wiley
Date: 26-08-2011
DOI: 10.1002/DC.21798
Abstract: Laboratory contamination of clinical specimens submitted for cytological examination is an on-going threat that requires continuous vigilance and quality control. We recently were confronted with a suspected fungal infection in a bone aspirate that was found to derive from contaminated Hanks solution used in cytological preparation. Diff-Quik staining of air-dried cytocentrifuged preparations of Hanks fluid as well as any other suspect laboratory fluid is recommended as a routine quality control procedure.
Publisher: Informa UK Limited
Date: 09-2002
Publisher: Wiley
Date: 23-01-2014
DOI: 10.1111/CYT.12129
Publisher: Elsevier BV
Date: 08-2023
Publisher: Informa UK Limited
Date: 12-2008
Publisher: Informa UK Limited
Date: 09-2007
Publisher: Elsevier BV
Date: 02-2017
Publisher: Informa UK Limited
Date: 04-03-2019
Publisher: Informa UK Limited
Date: 03-2005
Publisher: Informa UK Limited
Date: 25-08-2022
Publisher: Wiley
Date: 05-1998
DOI: 10.1046/J.1365-2559.1998.0358B.X
Abstract: Intraoperative fluorescence imaging is currently used in a variety of surgical fields for four main purposes: visualising anatomy, assessing tissue perfusion, identifying/localising cancer and mapping lymphatic systems. To establish evidence-based guidance for research and practice, understanding the state of research on fluorescence imaging in different surgical fields is needed. We evaluated the evidence on fluorescence imaging used to visualise anatomical structures using the IDEAL framework, a framework designed to describe the stages of innovation in surgery and other interventional procedures. IDEAL staging based on a thorough literature review. All publications on intraoperative fluorescence imaging for visualising anatomical structures reported in PubMed through 2020 were identified for five surgical procedures: cholangiography, hepatic segmentation, lung segmentation, ureterography and parathyroid identification. The IDEAL stage of research evidence was determined for each of the five procedures using a previously described approach. 225 articles (8427 cases) were selected for analysis. Current status of research evidence on fluorescence imaging was rated IDEAL stage 2a for ureterography and lung segmentation, IDEAL 2b for hepatic segmentation and IDEAL stage 3 for cholangiography and parathyroid identification. Enhanced tissue identification rates using fluorescence imaging relative to conventional white-light imaging have been documented for all five procedures by comparative studies including randomised controlled trials for cholangiography and parathyroid identification. Advantages of anatomy visualisation with fluorescence imaging for improving short-term and long-term postoperative outcomes also were demonstrated, especially for hepatobiliary surgery and (para)thyroidectomy. No adverse reactions associated with fluorescent agents were reported. Intraoperative fluorescence imaging can be used safely to enhance the identification of anatomical structures, which may lead to improved postoperative outcomes. Overviewing current research knowledge using the IDEAL framework aids in designing further studies to develop fluorescence imaging techniques into an essential intraoperative navigation tool in each surgical field.
Publisher: Informa UK Limited
Date: 22-11-2010
DOI: 10.3109/10520295.2010.527859
Abstract: Tissue microarrays place tens to hundreds of formalin fixed, paraffin embedded tissue cores into a paraffin block in a systematic grid pattern that permits their simultaneous evaluation in a single section. The fragmented nature of the tissue cores often makes sectioning of tissue microarrays difficult so that the resulting disks of tissue lose their shape, fracture or fall out of the paraffin section altogether. We have evaluated an alternative sectioning protocol for stabilizing the tissue microarray surface by placing an adhesive tape "window" over the face of the paraffin block prior to sectioning. Once sectioned, the tape/sections are transferred directly onto coated microscope slides, thereby avoiding routine floating of sections on a water bath. After sectioning with either the tape transfer or standard protocols, slides were stained either using hematoxylin and eosin or immunohistochemistry using antibodies to S-100 protein and the tissue specific antigens, keratin (AE1/3) and the leukocyte common antigen CD45. We found that the tape method produced thicker sections that were darker and more densely packed with loss of tissue definition compared to sections prepared using water bath flotation. Quantitative image analysis of immunohistochemical staining demonstrated that the tape method produced a higher incidence of nonspecific staining, which raised the potential for false positive staining.
Publisher: Informa UK Limited
Date: 2002
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 23-06-2020
Publisher: Maney Publishing
Date: 09-2010
Publisher: Informa UK Limited
Date: 03-04-2017
Publisher: Informa UK Limited
Date: 02-10-2017
Publisher: Informa UK Limited
Date: 03-04-2019
Publisher: MDPI AG
Date: 28-02-2014
Publisher: American Association for Cancer Research (AACR)
Date: 04-2012
DOI: 10.1158/1538-7445.AM2012-42
Abstract: Neuroblastoma (NB) is the most common extracranial childhood malignant solid tumour which shows remarkable biological heterogeneity. It has been speculated that schwann cells influence NB tumour growth, by secreting as yet to be identified ‘soluble factors’ that serve as anti-proliferative and differentiation signals for neuronal cells. Several gene expression studies indicate that interleukin 7 (IL7) has increased expression in NB specifically in tumours with a better prognosis, making IL7 a key candidate for this soluble factor. In this study, the expression of IL7, IL7 receptor (IL7R) and its downstream signalling proteins, including the Janus Kinases (JAK1 and JAK3), Signal Transducers and Activators of Transcription (STAT5) and Phosphorylated STAT5 (pSTAT5) were analysed using immunohistochemical stains in a cohort of 100 patients with stroma poor NB and 24 with stroma rich ganglioneuroma (GN). The expression of these proteins were correlated to the expression of the diagnostic markers S100 (stromal marker), NB84 (neuroblast marker) and CD99 (negative marker for NB). The immunohistochemical analysis showed that IL7 expression was strongly positive exclusively in schwannian stroma in both favourable and unfavourable histology whilst staining ganglionic cells as well. The levels of expression, as quantified by specialised digital image analysis algorithms (Aperio, USA), revealed IL7, IL7R, JAK1, STAT5 and pSTAT5 were all increased in GNs compared to NBs. There was no correlated change in expression levels of JAK3 in the tumours. Expression of pStat5 was found to be significantly reduced (t-test, p=0.00013) in stroma poor NB compared to GN. Our findings implicate IL7 within the schwannian stroma of the tumour architecture as having a paracrine signalling effect on neighbouring neuroblasts which may provide the anti-proliferative and differentiation signals postulated. Further investigation of the importance of IL7 in neuroblastoma will define the biological significance of the IL7-Stat5 signalling cascade and whether targeting the specific IL7 signalling pathway proteins will raise novel treatment strategies. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research 2012 Mar 31-Apr 4 Chicago, IL. Philadelphia (PA): AACR Cancer Res 2012 (8 Suppl):Abstract nr 42. doi:1538-7445.AM2012-42
Publisher: Wiley
Date: 24-01-2021
DOI: 10.1111/CUP.13959
Publisher: SAGE Publications
Date: 2015
Abstract: Parvovirus B19 infection causes 5% to 15% of cases of nonimmune hydrops fetalis. The aim of our study was to evaluate the use of immunohistochemistry in diagnosing parvovirus infection in fetal and placental tissue during routine fetal and perinatal autopsies. Histology slides of 20 cases of confirmed parvovirus infection were reviewed, and immunohistochemistry was applied to selected blocks of fetal and placental tissue. Immunohistochemistry was positive in all 20 cases, and histologic viral inclusions were seen in 19 cases. Immunohistochemical staining was closely correlated with histology and was more sensitive than histology in detecting virally infected cells, especially in autolyzed tissue. All cases also had confirmatory evidence of parvovirus infection by polymerase chain reaction of fetal liver and positive maternal serology, where it was available. We conclude that parvovirus immunohistochemistry is a reliable method for diagnosing parvovirus infection, especially in autolyzed tissue where histologic assessment may be suboptimal.
Publisher: Informa UK Limited
Date: 20-03-2018
No related grants have been discovered for Anthony Henwood.