ORCID Profile
0000-0001-5850-8470
Current Organisation
Theraproteins Lda
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Publisher: Springer Science and Business Media LLC
Date: 09-05-2015
Publisher: Springer Science and Business Media LLC
Date: 10-01-2014
DOI: 10.1186/SCRT394
Publisher: SAGE Publications
Date: 05-2015
Abstract: Mesenchymal stromal cells (MSCs) play an important role in tissue regeneration mainly through the secretion of trophic factors that enhance the repair of damaged tissues. The main goal of this work was to study the paracrine mechanisms by which an umbilical cord tissue-derived MSC population (UCX ® ) promotes the migration capacity of human dermal fibroblasts and keratinocytes, which is highly relevant for skin regeneration. Furthermore, the differences between paracrine activities of MSCs from the umbilical cord tissue and the bone marrow (BM-MSCs) were also evaluated. In vitro scratch assays revealed that conditioned media (CM) obtained from both growing and stationary-phase UCX ® cultures induced human dermal fibroblast (HDF) and keratinocyte (HaCaT) migration. These assays showed that the motogenic activity of UCX ® CM to HaCaTs was significantly higher than to HDFs, in opposition to the effect seen with CM produced by BM-MSCs that preferentially induced HDF migration. Accordingly, a comparative quantification of key factors with vital importance in the consecutive stages of wound healing revealed very different secretome profiles between UCX ® and BM-MSCs. The relatively higher UCX ® expression of EGF, FGF-2, and KGF strongly supports early induction of keratinocyte migration and function, whereas the UCX ® -specific expression of G-CSF suggested additional roles in mobilization of healing-related cells including CD34 - /CD45 - precursors (MSCs) known to be involved in tissue regeneration. Accordingly, in vitro chemotaxis assays and an in vivo transplantation model for chemoattraction confirmed that UCX ® are chemotactic to CD34 - /CD45 - BM-MSCs via a cell-specific mobilization mechanism mediated by G-CSF. Overall, the results strongly suggest different paracrine activities between MSCs derived from different tissue sources, revealing the potential of UCX ® to extend the regenerative capacity of the organism by complementing the role of endogenous BM-MSCs.
Publisher: Walter de Gruyter GmbH
Date: 05-07-2023
DOI: 10.2478/AMNS.2023.2.00011
Abstract: The author proposes a model evaluation based on the GA-SVM model to better understand the evaluation of the company’s relationship and core competitiveness. The system index is reduced by relative gray analysis, the support vector machine model is optimized by a genetic algorithm, and the specific algorithm steps are introduced. Select models from the top 100 enterprises in China’s construction industry in 2020 published by China Construction Industry Market, using relative gray to reduce the measure, and then use the genetic algorithm support vector machine (GA-SVM) model for the training model to achieve the evaluation of the core competencies of the target construction technique business. The experimental results show that the relative error of prediction of the GA-SVM(Genetic algorithm-support vector machine) model for the evaluation of the competitive core of the business is not more than 5, which meets the should be made of accurate predictions. Therefore, choosing the Gaussian radial basis kernel function (RBF) as the kernel function of the GA-SVM intelligent evaluation model is good. It proves that the GA-SVM model can analyze the relationship between the role and the important competition.
Location: Portugal
Location: Portugal
No related grants have been discovered for Helder Cruz.