ORCID Profile
0000-0001-8295-2950
Current Organisations
University of Western Australia
,
Otto von Guericke Universität Magdeburg
,
Western Australian Museum
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Publications
Conspicuously concealed: revision of the arid clade of theGehyra variegata(Gekkonidae) group in Western Australia using an integrative molecular and morphological approach, with the description
Publisher: PeerJ
Date: 19-07-2018
DOI: 10.7717/PEERJ.5334
Abstract: The methods used to detect and describe morphologically cryptic species have advanced in recent years, owing to the integrative nature of molecular and morphological techniques required to elucidate them. Here we integrate recent phylogenomic work that sequenced many genes but few in iduals, with new data from mtDNA and morphology from hundreds of gecko specimens of the Gehyra variegata group from the Australian arid zone. To better understand morphological and geographical boundaries among cryptic forms, we generated new sequences from 656 Gehyra in iduals, largely assigned to G. variegata group members over a wide area in Western Australia, with especially dense s ling in the Pilbara region, and combined them with 566 Gehyra sequences from GenBank, resulting in a dataset of 1,222 specimens. Results indicated the existence of several cryptic species, from new species with diagnostic morphological characters, to cases when there were no useful characters to discriminate among genetically distinctive species. In addition, the cryptic species often showed counter-intuitive distributions, including broad sympatry among some forms and short range endemism in other cases. Two new species were on long branches in the phylogram and restricted to the northern Pilbara region: most records of the moderately sized G. incognita sp. nov. are near the coast with isolated inland records, whereas the small-bodied saxicoline G. unguiculata sp. nov. is only known from a small area in the extreme north of the Pilbara. Three new species were on shorter branches in the phylogram and allied to G. montium . The moderately sized G. crypta sp. nov. occurs in the western and southern Pilbara and extends south through the Murchison region this species was distinctive genetically, but with wide overlap of characters with its sister species, G. montium . Accordingly, we provide a table of diagnostic nucleotides for this species as well as for all other species treated here. Two small-bodied species occur in isolated coastal regions: G. capensis sp. nov. is restricted to the North West Cape and G. ocellata sp. nov. occurs on Barrow Island and other neighbouring islands. The latter species showed evidence of introgression with the mtDNA of G. crypta sp. nov., possibly due to recent connectivity with the mainland owing to fluctuating sea levels. However, G. ocellata sp. nov. was more closely related to G. capensis sp. nov. in the phylogenomic data and in morphology. Our study illustrates the benefits of combining phylogenomic data with extensive screens of mtDNA to identify large numbers of in iduals to the correct cryptic species. This approach was able to provide sufficient s les with which to assess morphological variation. Furthermore, determination of geographic distributions of the new cryptic species should greatly assist with identification in the field, demonstrating the utility of s ling large numbers of specimens across wide areas.
Patterns of gene flow in two species of eel-tailed catfish, Neosilurus hyrtlii and Porochilus argenteus (Siluriformes: Plotosidae), in western Queensland's dryland rivers
Publisher: Oxford University Press (OUP)
Date: 24-02-2006
Author Correction: Tissue-resident memory CD8+ T cells promote melanoma–immune equilibrium in skin
Publisher: Springer Science and Business Media LLC
Date: 02-2019
DOI: 10.1038/S41586-019-0958-0
Abstract: Panel j was inadvertently labelled as panel k in the caption to Fig. 4. Similarly, 'Fig. 4k' should have been 'Fig. 4j' in the sentence beginning 'TNF-α-deficient gBT-I cells were…'. In addition, the surname of author Umaimainthan Palendira was misspelled 'Palendria'. These errors have been corrected online.
The effect of landscape processes upon gene flow and genetic diversity in an Australian freshwater fish, Neosilurus hyrtlii
Publisher: Wiley
Date: 19-03-2008
Diverse Anti-Tumor Immune Potential Driven by Individual IFNα Subtypes
Publisher: Frontiers Media SA
Date: 03-04-2020
CD8+XCR1neg Dendritic Cells Express High Levels of Toll-Like Receptor 5 and a Unique Complement of Endocytic Receptors
Publisher: Frontiers Media SA
Date: 16-01-2019
IFNβ Is a Potent Adjuvant for Cancer Vaccination Strategies.
Publisher: Frontiers Media SA
Date: 06-09-2021
DOI: 10.3389/FIMMU.2021.735133
Abstract: Cancer vaccination drives the generation of anti-tumor T cell immunity and can be enhanced by the inclusion of effective immune adjuvants such as type I interferons (IFNs). Whilst type I IFNs have been shown to promote cross-priming of T cells, the role of in idual subtypes remains unclear. Here we systematically compared the capacity of distinct type I IFN subtypes to enhance T cell responses to a whole-cell vaccination strategy in a pre-clinical murine model. We show that vaccination in combination with IFNβ induces significantly greater expansion of tumor-specific CD8 + T cells than the other type I IFN subtypes tested. Optimal expansion was dependent on the presence of XCR1 + dendritic cells, CD4 + T cells, and CD40/CD40L signaling. Therapeutically, vaccination with IFNβ delayed tumor progression when compared to vaccination without IFN. When vaccinated in combination with anti-PD-L1 checkpoint blockade therapy (CPB), the inclusion of IFNβ associated with more mice experiencing complete regression and a trend in increased overall survival. This work demonstrates the potent adjuvant activity of IFNβ, highlighting its potential to enhance cancer vaccination strategies alone and in combination with CPB.
CD4+ T cell-induced inflammatory killing controls immune evasive tumours
Publisher: Research Square Platform LLC
Date: 29-06-2022
DOI: 10.21203/RS.3.RS-1764653/V1
Abstract: Current clinically applied cancer immunotherapies largely focus on the ability of CD8+ cytolytic T-cells to directly recognise and kill tumour cells1–3. These strategies are limited by the emergence of MHC-I-deficient or IFN-unresponsive tumour cells and the development of an immunosuppressive tumour microenvironment4–6. CD4+ effector T-cells can contribute to tumour immune defence independent of CD8+ T-cells. However, the potential and the mechanisms of CD4+ T-cell-mediated anti-tumour immunity are incompletely understood7–12. Here, we show how an indirect CD4+ T-cell-mediated mode of action, that is fundamentally different from CD8+ T-cells, enables the eradication of tumours that would otherwise escape direct T-cell targeting. CD4+ effector T-cells preferentially cluster at tumour invasive margins where they engage in antigen-specific interactions with MHC-II+CD11c+ cells, while CD8+ T-cells briskly infiltrate tumour tissues. CD4+ T-cells and innate immune stimulation reprogram the tumour-associated inflammatory monocyte network towards IFN-activated antigen-presenting and tumouricidal effector phenotypes. This results in an lification loop driving the release of T-cell-derived IFNγ and myeloid cell-derived nitric oxide which cooperatively induce apoptotic death of MHC-deficient and IFN-unresponsive tumour cells that escape cytolytic CD8+ T-cell therapy. Exploiting the ability of CD4+ T-cells to orchestrate indirect inflammatory killing of tumour cells complements the direct cytolytic activity of T-cells to advance cancer immunotherapies.
Acquired resistance during adoptive cell therapy by transcriptional silencing of immunogenic antigens.
Publisher: Informa UK Limited
Date: 03-06-2019
CD4+ T cell-induced inflammatory cell death controls immune-evasive tumours
Publisher: Springer Science and Business Media LLC
Date: 14-06-2023
DOI: 10.1038/S41586-023-06199-X
Abstract: Most clinically applied cancer immunotherapies rely on the ability of CD8 + cytolytic T cells to directly recognize and kill tumour cells 1–3 . These strategies are limited by the emergence of major histocompatibility complex (MHC)-deficient tumour cells and the formation of an immunosuppressive tumour microenvironment 4–6 . The ability of CD4 + effector cells to contribute to antitumour immunity independently of CD8 + T cells is increasingly recognized, but strategies to unleash their full potential remain to be identified 7–10 . Here, we describe a mechanism whereby a small number of CD4 + T cells is sufficient to eradicate MHC-deficient tumours that escape direct CD8 + T cell targeting. The CD4 + effector T cells preferentially cluster at tumour invasive margins where they interact with MHC-II + CD11c + antigen-presenting cells. We show that T helper type 1 cell-directed CD4 + T cells and innate immune stimulation reprogramme the tumour-associated myeloid cell network towards interferon-activated antigen-presenting and iNOS-expressing tumouricidal effector phenotypes. Together, CD4 + T cells and tumouricidal myeloid cells orchestrate the induction of remote inflammatory cell death that indirectly eradicates interferon-unresponsive and MHC-deficient tumours. These results warrant the clinical exploitation of this ability of CD4 + T cells and innate immune stimulators in a strategy to complement the direct cytolytic activity of CD8 + T cells and natural killer cells and advance cancer immunotherapies.
Evidence for multiple historical colonizations of an endoreic drainage basin by an Australian freshwater fish
Publisher: Wiley
Date: 10-2011
DOI: 10.1111/J.1095-8649.2011.03088.X
Abstract: The contemporary and historical colonization capacity of an Australian freshwater fish, north-west glassfish Ambassis sp., was tested using mtDNA sequence data and six newly developed microsatellite loci in an endoreic basin in central Australia. Overall, Ambassis sp. exhibited weak genetic structure within catchments, suggesting some capacity to recolonize extirpated waterholes after disturbance. Genetic structure revealed that the historical pattern of connectivity among catchments in the Lake Eyre Basin was dramatically different from other species studied in this region. Two highly ergent clades were detected in separate catchments in the basin. mtDNA from in iduals s led in catchments north of the Lake Eyre Basin suggest that Ambassis sp. has colonized on two separate occasions from catchments in northern Australia, subsequently generating two highly ergent lineages.
High levels of genetic structure in the Australian freshwater fish, Ambassis macleayi
Publisher: University of Chicago Press
Date: 09-2010
DOI: 10.1899/09-093.1
Identifying the optimal donor for natural killer cell adoptive therapy to treat paediatric B- and T-cell acute lymphoblastic leukaemia
Publisher: Wiley
Date: 2020
DOI: 10.1002/CTI2.1151
Population demography and biology of a new species of giant spiny trapdoor spider (Araneae: Idiopidae: Euoplos) from inland Queensland: developing a ‘slow science’ study system to address a con
Publisher: Wiley
Date: 30-08-2018
DOI: 10.1111/AEN.12367
Impaired T cell proliferation byex vivoBET-inhibition impedes adoptive immunotherapy in a murine melanoma model
Publisher: Informa UK Limited
Date: 26-08-2019
Tissue-resident memory CD8+ T cells promote melanoma–immune equilibrium in skin
Publisher: Springer Science and Business Media LLC
Date: 31-12-2018
DOI: 10.1038/S41586-018-0812-9
Abstract: The immune system can suppress tumour development both by eliminating malignant cells and by preventing the outgrowth and spread of cancer cells that resist eradication
Related Organisations
Related Funding Activities
No related grants have been discovered for Anthony Buzzai.