ORCID Profile
0000-0001-9535-4925
Current Organisation
Fondation Mérieux
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Publisher: World Health Organization, Western Pacific Regional Office
Date: 10-01-2015
Publisher: American Society for Microbiology
Date: 15-07-2016
DOI: 10.1128/JVI.00093-16
Abstract: The discovery of influenza virus broadly neutralizing (BrN) antibodies prompted efforts to develop universal vaccines. Influenza virus stem-reactive (SR) broadly neutralizing antibodies have been detected by screening antibody phage display libraries. However, studies of SR BrN antibodies in human serum, and their association with natural infection, are limited. To address this, pre- and postpandemic sera from a prospective community cohort study in Vietnam were assessed for antibodies that inhibit SR BrN monoclonal antibody (MAb) (C179) binding to H1N1 pandemic 2009 virus (H1N1pdm09). Of 270 households, 33 with at least one confirmed H1N1pdm09 illness or at least two seroconverters were included. The included households comprised 71 infected and 41 noninfected participants. Sera were tested as 2-fold dilutions between 1:5 and 1:40. Fifty percent C179 inhibition (IC 50 ) titers did not exceed 10, although both IC 50 titers and percent C179 inhibition by sera diluted 1:5 or 1:10 correlated with hemagglutination inhibition (HI) and microneutralization (MN) titers (all P 0.001). Thirteen (12%) participants had detectable prepandemic IC 50 titers, but only one reached a titer of 10. This proportion increased to 44% after the pandemic, when 39 participants had a titer of 10, and 67% of infected compared to 44% of noninfected had detectable IC 50 titers ( P 0.001). The low levels of SR antibodies in prepandemic sera were not associated with subsequent H1N1pdm09 infection ( P = 0.241), and the higher levels induced by H1N1pdm09 infection returned to prepandemic levels within 2 years. The findings indicate that natural infection induces only low titers of SR antibodies that are not sustained. IMPORTANCE Universal influenza vaccines could have substantial health and economic benefits. The focus of universal vaccine research has been to induce antibodies that prevent infection by erse influenza virus strains. These so-called broadly neutralizing antibodies are readily detected in mice and ferrets after infection with a series of distinct influenza virus strains. The 2009 H1N1 pandemic provided an opportunity to investigate whether infection with a novel strain induced broadly neutralizing antibodies in humans. We found that broadly neutralizing antibodies were induced, but levels were low and poorly maintained. This could represent an obstacle for universal vaccine development and warrants further investigation.
Publisher: Elsevier BV
Date: 03-2019
Publisher: American Society of Tropical Medicine and Hygiene
Date: 03-05-2017
Publisher: Elsevier BV
Date: 2012
Publisher: Public Library of Science (PLoS)
Date: 24-03-2011
Publisher: Proceedings of the National Academy of Sciences
Date: 14-05-2013
Abstract: Dengue is the most prevalent arboviral disease of humans. The host and virus variables associated with dengue virus (DENV) transmission from symptomatic dengue cases ( n = 208) to Aedes aegypti mosquitoes during 407 independent exposure events was defined. The 50% mosquito infectious dose for each of DENV-1–4 ranged from 6.29 to 7.52 log10 RNA copies/mL of plasma. Increasing day of illness, declining viremia, and rising antibody titers were independently associated with reduced risk of DENV transmission. High early DENV plasma viremia levels in patients were a marker of the duration of human infectiousness, and blood meals containing high concentrations of DENV were positively associated with the prevalence of infectious mosquitoes 14 d after blood feeding. Ambulatory dengue cases had lower viremia levels compared with hospitalized dengue cases but nonetheless at levels predicted to be infectious to mosquitoes. These data define serotype-specific viremia levels that vaccines or drugs must inhibit to prevent DENV transmission.
Publisher: Oxford University Press (OUP)
Date: 27-09-2017
Publisher: Microbiology Society
Date: 12-2015
DOI: 10.1099/JGV.0.000298
Abstract: Human respiratory syncytial virus (RSV) is the major cause of lower respiratory tract infections in children 2 years of age. Little is known about RSV intra-host genetic ersity over the course of infection or about the immune pressures that drive RSV molecular evolution. We performed whole-genome deep-sequencing on 53 RSV-positive s les (37 RSV subgroup A and 16 RSV subgroup B) collected from the upper airways of hospitalized children in southern Vietnam over two consecutive seasons. RSV A NA1 and RSV B BA9 were the predominant genotypes found in our s les, consistent with other reports on global RSV circulation during the same period. For both RSV A and B, the M gene was the most conserved, confirming its potential as a target for novel therapeutics. The G gene was the most variable and was the only gene under detectable positive selection. Further, positively selected sites in G were found in close proximity to and in some cases overlapped with predicted glycosylation motifs, suggesting that selection on amino acid glycosylation may drive viral genetic ersity. We further identified hotspots and coldspots of intra-host genetic ersity in the RSV genome, some of which may highlight previously unknown regions of functional importance.
Publisher: Elsevier BV
Date: 09-2016
Publisher: Microbiology Society
Date: 12-2014
Abstract: During a hospital-based diarrhoeal disease study conducted in Ho Chi Minh City, Vietnam from 2009 to 2010, we identified four symptomatic children infected with G26P[19] rotavirus (RV) – an atypical variant that has not previously been reported in human gastroenteritis. To determine the genetic structure and investigate the origin of this G26P[19] strain, the whole genome of a representative ex le was characterized, revealing a novel genome constellation: G26–P[19]–I5–R1–C1–M1–A8–N1–T1–E1–H1. The genome segments were most closely related to porcine (VP7, VP4, VP6 and NSP1) and Wa-like porcine RVs (VP1–3 and NSP2–5). We proposed that this G26P[19] strain was the product of zoonotic transmission coupled with one or more reassortment events occurring in human and/or animal reservoirs. The identification of such strains has potential implications for vaccine efficacy in south-east Asia, and outlines the utility of whole-genome sequencing for studying RV ersity and zoonotic potential during disease surveillance.
Publisher: Oxford University Press (OUP)
Date: 07-2016
DOI: 10.1093/VE/VEW027
Publisher: Wiley
Date: 18-04-2017
DOI: 10.1111/ZPH.12362
Publisher: Public Library of Science (PLoS)
Date: 08-08-2016
Publisher: Public Library of Science (PLoS)
Date: 18-05-2007
Publisher: Elsevier BV
Date: 06-2014
Publisher: Cambridge University Press (CUP)
Date: 12-03-2015
DOI: 10.1017/S0950268815000187
Abstract: A reassortant swine-origin A(H3N2) virus (A/swine/BinhDuong/03-9/2010) was detected through swine surveillance programmes in southern Vietnam in 2010. This virus contains haemagglutinin and neuraminidase genes from a human A(H3N2) virus circulating around 2004–2006, and the internal genes from triple-reassortant swine influenza A viruses (IAVs). To assess population susceptibility to this virus we measured haemagglutination inhibiting (HI) titres to A/swine/BinhDuong/03-9/2010 and to seasonal A/Perth/16/2009 for 947 sera collected from urban and rural Vietnamese people during 2011–2012. Seroprevalence (HI ⩾ 40) was high and similar for both viruses, with 62·6% [95% confidence interval (CI) 59·4–65·7] against A/Perth/16/2009 and 54·6% (95% CI 51·4–57·8%) against A/swine/BinhDuong/03-9/2010, and no significant differences between urban and rural participants. Children aged years lacked antibodies to the swine origin H3 virus despite high seroprevalence for A/Perth/16/2009. These results reveal vulnerability to infection to this contemporary swine IAV in children aged years however, cross-reactive immunity in adults would likely limit epidemic emergence potential.
Publisher: Wiley
Date: 09-06-2017
DOI: 10.1111/ZPH.12364
Location: United States of America
Location: United States of America
No related grants have been discovered for Juliet Bryant.