ORCID Profile
0000-0002-9825-4425
Current Organisation
Universitas Padjadjaran
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Publisher: MDPI AG
Date: 06-06-2023
Abstract: Food preservation is one of the strategies taken to maintain the level of public health. Oxidation activity and microbial contamination are the primary causes of food spoilage. For health reasons, people prefer natural preservatives over synthetic ones. Syzygnium polyanthum is widely spread throughout Asia and is utilized as a spice by the community. S. polyanthum has been found to be rich in phenols, hydroquinones, tannins, and flavonoids, which are potential antioxidants and antimicrobial agents. Consequently, S. polyanthum presents a tremendous opportunity as a natural preservative. This paper reviews recent articles about S. polyanthum dating back to the year 2000. This review summarizes the findings of natural compounds presented in S. polyanthum and their functional properties as antioxidants, antimicrobial agents, and natural preservatives in various types of food.
Publisher: Springer Science and Business Media LLC
Date: 12-2008
Publisher: Universitas Padjadjaran
Date: 12-04-2018
Publisher: Universitas Padjadjaran
Date: 12-12-2015
Publisher: MDPI AG
Date: 25-07-2023
DOI: 10.3390/MOLECULES28155625
Abstract: Marennine, a blue pigment produced by the blue diatom Haslea ostrearia, is known to have some biological activities. This pigment is responsible for the greening of oysters on the West Coast of France. Other new species of blue diatom, H. karadagensis, H. silbo sp. inedit., H. provincialis sp. inedit, and H. nusantara, also produce marennine-like pigments with similar biological activities. Aside from being a potential source of natural blue pigments, H. ostrearia-like diatoms present a commercial potential for the aquaculture, food, cosmetics, and health industries. Unfortunately, for a hundred years, the exact molecular structure of this bioactive compound has remained a mystery. A lot of hypotheses regarding the chemical structure of marennine have been proposed. The recent discovery of this structure revealed that it is a macromolecule, mainly carbohydrates, with a complex composition. In this study, some glycoside hydrolases were used to digest marennine, and the products were further analyzed using nuclear magnetic resonance (NMR) and mass spectroscopy (MS). The reducing sugar assay showed that marennine was hydrolyzed only by endo-1,3-β-glucanase. Further insight into the structure of marennine was provided by the spectrum of 1H NMR, MS, a colorimetric assay, and a computational study, which suggest that the chemical structure of marennine contains 1,3-β-glucan.
Publisher: College of Science for Women
Date: 12-2020
DOI: 10.21123/BSJ.2020.17.4.1198
Abstract: This study was aimed to develop an optimized Dy determination method using differential pulse voltammetry (DPV). The Plackett-Burman (PB) experimental design was used to select significant factors that affect the electrical current response, which were further optimized using the response surface method-central composite design (RSM-CCD). The type of electrolyte solution and litude modulation were found as two most significant factors, among the nine factors tested, which enhance the current response based on PB design. Further optimization using RSM-CCD shows that the optimum values for the two factors were 0.1046 M and 0.1082 V respectively. When the optimum conditions were applied for Dy determination good recovery and precision were achieved with values of 91.58%, and 99.80%, respectively. The detection limit and quantification limit of the method were 1.4322 mg/L and of 4.7741 mg/L, respectively.
Publisher: Hindawi Limited
Date: 05-12-2017
DOI: 10.1155/2017/4086845
Abstract: Starch is a polymeric carbohydrate composed of glucose. As a source of energy, starch can be degraded by various amylolytic enzymes, including α -amylase. In a large-scale industry, starch processing cost is still expensive due to the requirement of high temperature during the gelatinization step. Therefore, α -amylase with raw starch digesting ability could decrease the energy cost by avoiding the high gelatinization temperature. It is known that the carbohydrate-binding module (CBM) and the surface-binding site (SBS) of α -amylase could facilitate the substrate binding to the enzyme’s active site to enhance the starch digestion. These sites are a noncatalytic module, which could interact with a lengthy substrate such as insoluble starch. The major interaction between these sites and the substrate is the CH i-stacking interaction with the glucose ring. Several mutation studies on the Halothermothrix orenii , SusG Bacteroides thetaiotamicron , Barley , Aspergillus niger , and Saccharomycopsis fibuligera α -amylases have revealed that the stacking interaction through the aromatic residues at the SBS is essential to the starch adsorption. In this review, the SBS in various α -amylases is also presented. Therefore, based on the structural point of view, SBS is suggested as an essential site in α -amylase to increase its catalytic activity, especially towards the insoluble starch.
Publisher: Springer Science and Business Media LLC
Date: 27-11-2017
DOI: 10.1007/S11274-017-2380-9
Abstract: Membrane lipid unsaturation index and membrane fluidity have been related to yeast ethanol stress tolerance in published studies, however findings have been inconsistent. In this study, viability reduction on exposure to 18% (v/v) ethanol was compared to membrane fluidity determined by laurdan generalized polarization. Furthermore, in the determination of viability reduction, we examined the effectiveness of two methods, namely total plate count and methylene violet staining. We found a strong negative correlation between ethanol tolerance and membrane fluidity, indicated by negative Pearson correlation coefficients of - 0.79, - 0.65 and - 0.69 for Saccharomyces cerevisiae strains A12, PDM and K7, respectively. We found that lower membrane fluidity leads to higher ethanol tolerance, as indicated by decreased viability reduction and higher laurdan generalized polarization in respiratory phase compared to respiro-fermentative phase cells. Total plate count better differentiated ethanol tolerance of yeast cells in different growth phases, while methylene violet staining was better to differentiate ethanol tolerance of the different yeast strains at a particular culture phase. Hence, both viability assessment methods have their own advantages and limitations, which should be considered when comparing stress tolerance in different situations.
Publisher: Universitas Padjadjaran
Date: 15-04-2020
Publisher: IOP Publishing
Date: 02-2017
Publisher: Elsevier BV
Date: 04-2019
Publisher: Sunan Gunung Djati State Islamic University of Bandung
Date: 10-05-2020
Abstract: Antosianin merupakan pigmen pewarna alami yang berwarna dan dapat berperan sebagai antioksidan. Senyawa ini dapat digunakan sebagai salah satu bahan tambahan makanan sebagai pewarna. Selain peranannya sebagai zat warna, antosianin juga dapat berperan sebagai senyawa antioksidan, sehingga ketika ditambahkan pada makanan akan menghasilkan suatu pangan fungsional. Salah satu bahan yang kaya akan antosianin adalah ubi jalar ungu (Ipomoea batatas L.). Tujuan dari penelitian ini adalah untuk mengekstraski antosianin dari ubi jalar ungu. Metode yang dilakukan meliputi preparasi antosianin melalui maserasi dengan etanol, pemekatan dengan teknik evaporasi vakum ekstrak ubi ungu, karakterisasi spektrum sinar t ak serta penentuan residu etanol pada ekstrak. Hasil yang diperoleh menunjukkan bahwa dari 500 g ubi jalar ungu segar yang diekstraksi dengan 1 L etanol diperoleh 8 g pasta ekstrak ubi jalar ungu. Karakterisasi dengan spektrofotometri pada pajang gelombang sinar t ak menghasilkan spektrum yang sama dengan spektrum yang telah dipublikasikan sebelumnya. Ekstrak yang diperoleh memiliki aktivitas antioksidan yang cukup tinggi dengan nilai konsentrasi inhibisi 50 (IC50) sebesar 41,1±7,3 ppm. Karena tujuan ekstraksi ini adalah untuk aplikasi pada bidang pangan, residu etanol yang terdapat pada ekstrak ditentukan dengan metode enzimatik menggunakan alkohol dehidrogenase dan diperoleh residu etanol sebanyak 0,122 ±0,033% v/v.
Publisher: Universitas Padjadjaran
Date: 12-12-2019
Publisher: Informa UK Limited
Date: 06-2019
DOI: 10.2147/AABC.S198110
Publisher: Universitas Padjadjaran
Date: 12-12-2020
Publisher: Universitas Sarjanawiyata Tamansiswa
Date: 03-08-2020
Abstract: Cream cheese adalah salah satu jenis keju yang kaya akan nutrisi dan dibuat dengan metode koagulasi asam, termasuk menggunakan fermentasi dengan bakteri asam laktat. Cream cheese memiliki tekstur yang lembut, halus, putih dan creamy serta memiliki cita rasa asam. Untuk meningkatkan kualitas cream cheese dapat ditambah dengan kulit ari psyllium (Platago ovata) dan susu full cream yang masing-masing dapat berperan sebagai stabilizer dan memperbaiki cita rasa. Penelitian ini betujuan untuk menentukan pengaruh kulit ari psyllium dan susu full cream terhadap kandungan air dan abu produk cream cheese. Fermentasi susu dilakukan dengan menggunakan Lactobacillus bulgaricus dengan variasi penambahan kulit ari psyllium (0.1-0.5%) dan susu full cream (8-16%). Percobaan dilakukan dengan menggunakan response surface method-central composite design. Hasil eksperimen menunjukkan bahwa kulit ari psyllium memberikan efek yang signifikan terhadap kandungan air (p = 0,035) dan abu (p = 0.009) produk cream cheese. Persamaan yang diperoleh pada eksperimen ini dapat digunakan untuk mengatur kandungan air dan abu produk cream cheese yang dibuat.
Publisher: SAGE Publications
Date: 2017
Abstract: α-Amylase is one of the important enzymes in the starch-processing industry. However, starch processing requires high temperature, thus resulting in high cost. The high adsorptivity of α-amylase to the substrate allows this enzyme to digest the starch at a lower temperature. α-Amylase from Saccharomycopsis fibuligera R64 (Sfamy R64), a locally sourced enzyme from Indonesia, has a high amylolytic activity but low starch adsorptivity. The objective of this study was to design a computational model of Sfamy R64 with increased starch adsorptivity using bioinformatics method. The model structure of Sfamy R64 was compared with the positive control, ie, Aspergillus niger α-amylase. The structural comparison showed that Sfamy R64 lacks the surface-binding site (SBS). An SBS was introduced to the structure of Sfamy R64 by S383Y/S386W mutations. The dynamics and binding affinity of the SBS of mutant to the substrate were also improved and comparable with that of the positive control.
Publisher: MDPI AG
Date: 26-09-2022
Abstract: Rice (Oryza sativa L.) is a primary food that is widely consumed throughout the world, especially in Asian countries. The two main subspecies of rice are japonica and indica which are different in physical characteristics. In general, both indica and japonica rice consist of three types of grain colors, namely white, red, and black. Furthermore, rice and rice by-products contain secondary metabolites such as phenolic compounds, flavonoids, and tocopherols that have bioactivities such as antioxidants, antimicrobial, cancer chemopreventive, antidiabetic, and hypolipidemic agents. The existence of health benefits in rice bran, especially as antioxidants, gives rice bran the opportunity to be used as a functional food. Most of the bioactive compounds in plants are found in bound form with cell wall components such as cellulose and lignin. The process of releasing bonds between bioactive components and cell wall components in rice bran can increase the antioxidant capacity. Fermentation and treatment with enzymes were able to increase the total phenolic content, total flavonoids, tocotrienols, tocopherols, and γ-oryzanol in rice bran.
No related grants have been discovered for Safri Ishmayana.