ORCID Profile
0000-0001-5373-8701
Current Organisations
Université Laval
,
Canadian Museum of Nature
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Publisher: Wiley
Date: 26-01-2020
DOI: 10.1111/JZO.12756
Publisher: Springer Science and Business Media LLC
Date: 13-10-2022
DOI: 10.1186/S13071-022-05446-W
Abstract: Bartonella are intracellular bacteria that are transmitted via animal scratches, bites and hematophagous arthropods. Rodents and their associated fleas play a key role in the maintenance of Bartonella worldwide, with 22 species identified in rodent hosts. No studies have addressed the occurrence and ersity of Bartonella species and vectors for small mammals in Arctic and Subarctic ecosystems, which are increasingly impacted by invasive species and climate change. In this study, we characterized the ersity of rodent fleas using conventional PCR targeting the mitochondrial cytochrome c oxidase II gene (COII) and Bartonella species in rodents and shrews ( n = 505) from northern Canada using conventional PCR targeting the ITS (intergenic transcribed spacer) region and gltA (citrate synthase) gene. Metagenomic sequencing of a portion of the gltA gene was completed on a subset of 42 rodents and four rodent flea pools. Year, total summer precipitation the year prior to s ling, average minimum spring temperature and small mammal species were significant factors in predicting Bartonella positivity. Occurrence based on the ITS region was more than double that of the gltA gene and was 34% ( n = 349) in northern red-backed voles, 35% ( n = 20) in meadow voles, 37% ( n = 68) in deer mice and 31% ( n = 59) in shrews. Six species of Bartonella were identified with the ITS region, including B. grahamii, B. elizabethae , B. washoensis , Candidatus B. rudakovii, B. doshiae , B. vinsonii subsp. berkhoffii and subsp. arupensis . In addition, 47% ( n = 49/105) of ITS licons had 97% identity to sequences in GenBank, possibly due to a limited reference library or previously unreported species. An additional Bartonella species ( B. heixiaziensis ) was detected during metagenomic sequencing of the gltA gene in 6/11 rodents that had ITS sequences with 97% identity in GenBank, highlighting that a limited reference library for the ITS marker likely accounted for low sequence similarity in our specimens. In addition, one flea pool from a northern red-backed vole contained multiple species ( B. grahamii and B. heixiaziensis ). Our study calls attention to the usefulness of a combined approach to determine the occurrence and ersity of Bartonella communities in hosts and vectors.
Publisher: Canadian Science Publishing
Date: 12-2020
DOI: 10.1139/AS-2019-0023
Abstract: Morphological differentiation and relationships among collared lemmings (Dicrostonyx Gloger, 1841) remain unclear. This issue is particularly important in the Kivalliq Region, Nunavut, Canada, where Dicrostonyx groenlandicus (Traill, 1823) and Dicrostonyx richardsoni Merriam, 1900 ranges overlap. Possible sympatry of both species obscures the taxonomic status of collared lemmings from this area. We compared 2D outline shapes of the skull and three upper molars of collared lemmings collected from seven areas of the Canadian Arctic, including specimens from the Baker Lake – Aberdeen Lake area, in the Kivalliq Region, and Dicrostonyx hudsonius (Pallas, 1778) as an outgroup. Multivariate analyses revealed two distinct groups when considering the molars: D. hudsonius, and the remainder of lemmings. Dicrostonyx richardsoni, D. groenlandicus, and the lemmings from the Baker Lake – Aberdeen Lake area showed significant differences, especially when considering skull shapes, thus suggesting three distinct groups. However, skull shapes proved inefficient in discriminating between species. These differences suggest that collared lemmings from the Baker Lake – Aberdeen Lake area may not be correctly assigned to either of the two species without further genetic evaluation. They also suggest that these lemmings should have a peculiar taxonomic status. Our study calls for further taxonomical investigations for collared lemmings from the overlapping distribution ranges of D. groenlandicus and D. richardsoni.
Location: Canada
Location: No location found
No related grants have been discovered for Dominique Fauteux.