ORCID Profile
0000-0003-4275-5519
Current Organisations
University of Queensland
,
Wellcome Sanger Institute
,
University of California, Irvine
,
University of Cambridge
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Elsevier BV
Date: 02-2013
Publisher: The Royal Society
Date: 17-11-2010
Abstract: Indoor residual spray (IRS) of insecticides and insecticide-treated bednets (ITNs) are the two most important malaria vector control tools in the tropical world. Application of both tools in the same locations is being implemented for malaria control in endemic and epidemic Africa. The two tools are assumed to have synergistic benefits in reducing malaria transmission because they both act at multiple stages of the transmission cycle. However, this assumption has not been rigorously examined, empirically or theoretically. Using mathematical modelling, we obtained the conditions for which a combination strategy can be expected to improve upon single control tools. Specifically, spraying of dichlorodiphenyltrichloroethane (DDT) in all houses where residents are not using ITNs can reduce transmission of malaria ( R 0 ) by up to 10 times more than the reduction achieved through ITNs alone. Importantly, however, we also show how antagonism between control tools can arise via interference of their modes of action. Repellent IRS reduces the likelihood that ITNs are contacted within sprayed houses and ITNs reduce the rate at which blood-fed mosquitoes rest on sprayed walls. For ex le, 80 per cent coverage of ITNs and DDT used together at the household level resulted in an R 0 of 11.1 when compared with an R 0 of 0.1 achieved with 80 per cent ITN coverage without DDT. While this undesired effect can be avoided using low-repellence pyrethroid chemicals for IRS, the extent of the potential benefits is also attenuated. We discuss the impact that this result will likely have on future efforts in malaria control combination strategy.
Publisher: Elsevier BV
Date: 11-2021
Publisher: Wiley
Date: 16-06-2011
DOI: 10.1002/PS.2218
Abstract: Insecticide discovery screens carried out on whole organisms screen for potency resulting from chemical activity at the target site. However, many potentially insecticidal compounds are naturally detoxified in vivo and do not make it to the target site. It is hypothesised that insect strains with their xenobiotic detoxification machinery compromised could be used to identify such compounds that normally fail to show up in screens these compounds could then be more rationally designed to increase their bioavailability. This strategy was tested with transgenic Drosophila lines with altered expression of Cyp6g1 and Dhr96. It was observed that Cyp6g1 knockdown transgenic lines have increased susceptibility to the test compound imidacloprid, while Dhr96 knockdown transgenic lines are resistant. Evidence was found for a systemic response to xenobiotic exposure, uncovered by piperonyl butoxide treatment and by gene expression profiling. Sex-specific gene expression regulated by DHR96 was also observed. The results confirm that this approach to chemical discovery could identify compounds that escape traditional screens. The complexity of the system means that a panel of single and multiple gene knockdown transgenic lines may be required.
Publisher: Springer Science and Business Media LLC
Date: 03-03-2016
Publisher: Springer Science and Business Media LLC
Date: 25-04-2022
DOI: 10.1038/S41467-022-29834-Z
Abstract: The Notch signalling pathway is a master regulator of cell fate transitions in development and disease. In the brain, Notch promotes neural stem cell (NSC) proliferation, regulates neuronal migration and maturation and can act as an oncogene or tumour suppressor. How NOTCH and its transcription factor RBPJ activate distinct gene regulatory networks in closely related cell types in vivo remains to be determined. Here we use Targeted DamID (TaDa), requiring only thousands of cells, to identify NOTCH and RBPJ binding in NSCs and their progeny in the mouse embryonic cerebral cortex in vivo. We find that NOTCH and RBPJ associate with a broad network of NSC genes. Repression of NSC-specific Notch target genes in intermediate progenitors and neurons correlates with decreased chromatin accessibility, suggesting that chromatin compaction may contribute to restricting NOTCH-mediated transactivation.
Publisher: Springer Science and Business Media LLC
Date: 29-04-2016
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Rebecca Yakob.