ORCID Profile
0000-0002-6043-2791
Current Organisations
The University of Edinburgh
,
University of Maryland Center for Environmental Science
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Publisher: Springer Science and Business Media LLC
Date: 2013
Publisher: Oxford University Press (OUP)
Date: 16-08-2017
Publisher: Springer Science and Business Media LLC
Date: 23-04-2001
Publisher: Inter-Research Science Center
Date: 2001
DOI: 10.3354/MEPS223121
Publisher: Springer Science and Business Media LLC
Date: 11-2001
DOI: 10.1007/S10126-001-0065-7
Abstract: The presence of Archaea in the Great Barrier Reef marine sponge Rhopaloeides odorabile was investigated by 16S ribosomal RNA community analysis of total DNA extracted from the sponge tissue. The 16S rRNA gene sequences corresponding to group I crenarchaeotes and group II euryarchaeotes were recovered from R. odorabile tissue. The location of archaeal cells within the sponge tissue was investigated using fluorescently labeled oligonucleotide probes. The presence of Archaea was confirmed within all regions of the sponge tissue from R. odorabile, with a significantly higher number of archaeal cells located in the pinacoderm than the mesohyl region. This is the first report of euryarchaeaotes associated with marine sponges.
Publisher: Wiley
Date: 02-2001
Publisher: Wiley
Date: 09-2016
DOI: 10.1136/VR.103729
Publisher: Elsevier BV
Date: 2000
Publisher: Inter-Research Science Center
Date: 2002
DOI: 10.3354/MEPS232305
Publisher: American Society for Microbiology
Date: 2001
DOI: 10.1128/AEM.67.1.434-444.2001
Abstract: Molecular techniques were employed to document the microbial ersity associated with the marine sponge Rhopaloeides odorabile . The phylogenetic affiliation of sponge-associated bacteria was assessed by 16S rRNA sequencing of cloned DNA fragments. Fluorescence in situ hybridization (FISH) was used to confirm the presence of the predominant groups indicated by 16S rDNA analysis. The community structure was extremely erse with representatives of the Actinobacteria , low-G+C gram-positive bacteria, the β- and γ-sub isions of the Proteobacteria , Cytophaga/Flavobacterium , green sulfur bacteria, green nonsulfur bacteria, planctomycetes, and other sequence types with no known close relatives. FISH probes revealed the spatial location of these bacteria within the sponge tissue, in some cases suggesting possible symbiotic functions. The high proportion of 16S rRNA sequences derived from novel actinomycetes is good evidence for the presence of an indigenous marine actinomycete assemblage in R. odorabile. High microbial ersity was inferred from low duplication of clones in a library with 70 representatives. Determining the phylogenetic affiliation of sponge-associated microorganisms by 16S rRNA analysis facilitated the rational selection of culture media and isolation conditions to target specific groups of well-represented bacteria for laboratory culture. Novel media incorporating sponge extracts were used to isolate bacteria not previously recovered from this sponge.
Location: United Kingdom of Great Britain and Northern Ireland
Location: United States of America
No related grants have been discovered for Damon Querry.