ORCID Profile
0000-0002-0630-1520
Current Organisation
University of Illinois at Urbana-Champaign College of Engineering
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Epigenetics (incl. Genome Methylation and Epigenomics) | Nanotechnology | Analytical Biochemistry | Nanobiotechnology |
Expanding Knowledge in the Biological Sciences | Expanding Knowledge in Technology
Publisher: American Chemical Society (ACS)
Date: 09-09-2010
DOI: 10.1021/JP1015406
Publisher: American Scientific Publishers
Date: 10-2012
Abstract: A one-step approach towards the synthesis of folic acid (FA) coated silver nanoparticles (AgNPs) and doxorubicin (DOX) for drug delivery is proposed. The FA-AgNPs were 23 +/- 2 nm in diameter and showed good monodispersity with a characteristic surface plasmon peak at 409 nm. Taking advantage of the enhanced Raman signal of FA on AgNPs and targeting specificity of FA to folate-receptor expressing cancer cells, the as-prepared FA-AgNPs showed excellent receptor-mediated cellular uptake, as demonstrated both by surface enhanced raman scattering (SERS) imaging and fluorescent lifetime imaging (FLIM). Next, a chemotherapeutic drug, DOX was attached to the FA-AgNPs surface through electrostatic bonding and the release of DOX from FA-AgNPs was monitored by FLIM. The release of DOX into the cytoplasm after 4 hours of incubation was clearly seen by FLIM. After 8 hours, the cells underwent cell death due to the release of DOX from nanoparticles. However, in control cell lines with low-expression levels of the FA receptor, no scattering signal from AgNPs could be observed from within the cells even after an incubation period of 24 hours. Our study shows that nanocarriers can be designed with various chemistries to study targeting and release of disease-specific drugs.
Publisher: CRC Press
Date: 19-12-2017
DOI: 10.1201/B16234
Publisher: American Chemical Society (ACS)
Date: 16-06-2010
DOI: 10.1021/NN100351H
Abstract: Nanoparticles are increasingly being used to investigate biological processes in various animal models due to their versatile chemical, unique optical, and multifunctional properties. In this report we address the biocompatibility and biodistribution of nanoparticle sensors used for Raman chemical imaging in live zebrafish (Danio rerio) embryos. Surface-enhanced Raman scattering (SERS) nanoprobes (NPs) comprising gold nanoparticles (AuNPs) as enhancing substrate and nonfluorescent Raman labels were synthesized and microinjected into zebrafish embryos at the one-cell stage. Raman mapping was performed to assess their distribution in various cell-types and tissues of developing embryo at five different stages between 6 and 96 hpf (hours post-fertilization). Biocompatibility and toxicity studies indicate that the NPs are not toxic and the embryos were found to exhibit normal morphological and gene expression in addition to the proper form and function of major organs such as the heart and vasculature (of 7 day old NPs injected zebrafish embryos). A multiplex in vivo detection protocol was developed by SERS imaging to demonstrate that multiple labels can be detected by Raman mapping in undifferentiated cells as they develop into distinct cell- and tissue-types. The present work is the first to report on multiplex Raman imaging of zebrafish embryos with potential implications in tracking tissue development and biological processes at single molecule sensitivity using appropriate target molecules in vivo.
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C0CC04140H
Abstract: SERS biosensor for sensitive and selective detection of lead ions (Pb(2+)) based on DNAzyme was developed by taking advantage of the specific catalytic reaction of DNAzyme upon binding to Pb(2+) ions. Detection was accomplished by SERS nanoprobe labeled with DNA and Raman reporters for signal lification.
Publisher: Elsevier BV
Date: 03-2011
Publisher: MDPI AG
Date: 02-12-2021
DOI: 10.3390/S21238050
Abstract: Organophosphates (OPs) are neurotoxic agents also used as pesticides that can permanently block the active site of the acetylcholinesterase (AChE). A robust and sensitive detection system of OPs utilising the enzyme mimic potential of the cysteamine capped gold nanoparticles (C-AuNPs) was developed. The detection assay was performed by stepwise addition of AChE, parathion ethyl (PE)-a candidate OP, acetylcholine chloride (ACh), C-AuNPs, and 3, 3′, 5, 5′-tetramethylbenzidine (TMB) in the buffer solution. The whole sensing protocol completes in 30–40 min, including both incubations. The Transmission Electron Microscopy (TEM) results indicated that the NPs are spherical and have an average size of 13.24 nm. The monomers of C-AuNPs exhibited intense catalytic activity (nanozyme) for the oxidization of TMB, revealed by the production of instant blue colour and confirmed by a sharp peak at 652 nm. The proposed biosensor’s detection limit and linear ranges were 5.8 ng·mL−1 and 11.6–92.8 ng·mL−1, respectively, for PE. The results strongly advocate that the suggested facile colorimetric biosensor may provide an excellent platform for on-site monitoring of OPs.
Publisher: Springer Science and Business Media LLC
Date: 07-12-2010
DOI: 10.1007/S00216-010-4453-6
Abstract: A rapid and sensitive method was developed here for separation and detection of multiple pathogens in food matrix by magnetic surface-enhanced Raman scattering (SERS) nanoprobes. Silica-coated magnetic probes (MNPs@SiO(2)) of ~100 nm in diameter were first prepared via the reverse microemulsion method using cetyltrimethylammonium bromide as a surfactant and tetraethyl orthosilicate as the silica precursor. The as-prepared MNPs@SiO(2) were functionalized with specific pathogen antibodies to first capture threat agents directly from a food matrix followed by detection using an optical approach enabled by SERS. In this scheme, pathogens were first immuno-magnetically captured with MNPs@SiO(2), and pathogen-specific SERS probes (gold nanoparticles integrated with a Raman reporter) were functionalized with corresponding antibodies to allow the formation of a sandwich assay to complete the sensor module for the detection of multiple pathogens in selected food matrices, just changing the kinds of Raman reporters on SERS probes. Here, up to two key pathogens, Salmonella enterica serovar Typhimurium and Staphylococcus aureus, were selected as a model to illustrate the probability of this scheme for multiple pathogens detection. The lowest cell concentration detected in spinach solution was 10(3) CFU/mL. A blind test conducted in peanut butter validated the limit of detection as 10(3) CFU/mL with high specificity, demonstrating the potential of this approach in complex matrices.
Publisher: American Chemical Society (ACS)
Date: 11-11-2011
DOI: 10.1021/NN2032177
Abstract: Recent advances in fluorescent metal nanoclusters have spurred tremendous interest in nanomedicine due to the ease of fabrication, excellent biocompatibility, and, more importantly, excellent wavelength-dependent tunability. Herein, we report our findings on fluorescent BSA-protected gold nanoclusters (AuNCs), ∼2 nm in size conjugated with Herceptin (AuNCs-Her), for specific targeting and nuclear localization in ErbB2 over-expressing breast cancer cells and tumor tissue as a novel fluorescent agent for simultaneous imaging and cancer therapy. More interestingly, we found that AuNCs-Her could escape the endolysosomal pathway and enter the nucleus of cancer cells to enhance the therapeutic efficacy of Herceptin. We elucidate the diffusion characteristics (diffusion time and number of diffusers) and concentration of the fluorescing clusters in the nucleus of live cells. Our findings also suggest that the nuclear localization effect of AuNCs-Her enhances the anticancer therapeutic efficacy of Herceptin as evidenced by the induction of DNA damage. This study not only discusses a new nanomaterial platform for nuclear delivery of drugs but also provides important insights on nuclear targeting for enhanced therapy.
Publisher: Royal Society of Chemistry (RSC)
Date: 2010
DOI: 10.1039/B919607B
Abstract: A multicomponent nanostructure comprising of gold nanorod-nanoparticle (AuNR-AuNP) composites was fabricated to detect thrombin at subnanomolar concentrations in diluted human blood serum. Simulation and experiments revealed that the strong electromagnetic coupling resonance at the nanorod-nanoparticle junction of these probes can be used to construct highly sensitive SERS aptasensors.
Publisher: The Royal Society
Date: 05-02-2013
Abstract: Single-molecule (SM) spectroscopy has been an exciting area of research offering significant promise and hope in the field of sensor development to detect targets at ultra-low levels down to SM resolution. To the experts and developers in the field of surface-enhanced Raman spectroscopy (SERS), this has often been a challenge and a significant opportunity for exploration. Needless to say, the opportunities and excitement of this multidisciplinary area impacts span the fields of physics, chemistry and engineering, along with a significant thrust in applications constituting areas in medicine, biology, environment and agriculture among others. In this review, we will attempt to provide a quick snapshot of the basics of SM-SERS, nanostructures and devices that can enable SM Raman measurement. We will conclude with a discussion on SERS implications in biomedical sciences.
Publisher: Proceedings of the National Academy of Sciences
Date: 12-05-2014
Abstract: Plasmonic and metamaterial devices require high-performance material building blocks, both plasmonic and dielectric, to be useful in any real-world application. Here, we develop both plasmonic and dielectric materials that can be grown epitaxially into ultrathin and ultrasmooth layers with sharp interfaces. We show that a superlattice consisting of titanium nitride as a plasmonic component behaves as an optical hyperbolic metamaterial and exhibits extremely high photonic density of states.
Publisher: Elsevier BV
Date: 02-2012
DOI: 10.1016/J.EJMECH.2011.12.036
Abstract: This study exhibits the fabrication of target-specific Gold nanorods (GNRs) coupled with an anti-tumorigenic apoptotic drug and provides tracking of the labeled particles as they migrate through cells and release their drug-load to targeted cancer cells. We utilize the photoluminescence property of GNRs and their ability to be conjugated with multiple agents to transform facile rods to a targeted drug delivery vehicle. GNRs of aspect ratio 2.8 were conjugated with a targeting ligand, folic acid and an anthracycline drug, Doxorubicin. The multifunctional nanorods were then used to target folate receptor expressing cancers cells for the delivery of a concentration dependent dosage of Doxorubicin (DOX). By utilizing the photoluminescence of GNRs and the innate fluorescence of DOX, multi-photon fluorescence lifetime imaging was utilized to monitor the uptake of functionalized nanorods, the release of the drug and its localization in living cells. We show that these nano-vehicles successfully targeted cancer cells over expressing folate receptors and showed low toxicity to control cell lines. Release of DOX was observed in the cytoplasmic region and after 16 h was found to be redistributed in the nucleus resulting in cell death. Our theragnostic approach demonstrates the fabrication of multifunctional GNRs for targeted drug delivery and monitoring of the drug and the vehicle by multi-photon microscopy using fluorescence intensity and lifetime imaging.
Location: United States of America
Start Date: 05-2023
End Date: 05-2026
Amount: $390,000.00
Funder: Australian Research Council
View Funded Activity