ORCID Profile
0000-0001-5706-3932
Current Organisations
Monash Institute of Pharmaceutical Sciences
,
Monash University
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Synthesis of Materials | Macromolecular and Materials Chemistry | Pharmaceutical Sciences | Pharmacology and Pharmaceutical Sciences | Structural Chemistry | Nanotechnology | Organic Chemistry | Physical Organic Chemistry | Organic Chemical Synthesis | Nanochemistry and Supramolecular Chemistry | Physical Chemistry (Incl. Structural) | Physical Chemistry Of Macromolecules | Environmental Technologies | Civil Engineering | Nanomaterials | Nanomedicine | Toxicology (Incl. Clinical Toxicology) | Nanotechnology | Macromolecular and Materials Chemistry not elsewhere classified | Membrane And Separation Technologies | Water And Sanitary Engineering | Bacteriology
ANZSRC Socio-Economic Objective (SEO)
Human Pharmaceutical Treatments (e.g. Antibiotics) | Expanding Knowledge in the Biological Sciences | Plastics in primary forms | Biological sciences | Chemical sciences | Expanding Knowledge in the Medical and Health Sciences | Land and water management | Veterinary Pharmaceutical Treatments (e.g. Antibiotics) | Water services and utilities | Expanding Knowledge in the Physical Sciences | Cancer and Related Disorders | Treatments (e.g. chemicals, antibiotics) |
Related Links
Publications
Biomimetic polymers responsive to a biological signaling molecule: Nitric oxide triggered reversible self-assembly of single macromolecular chains into nanoparticles
Publisher: Wiley
Date: 04-06-2014
Abstract: Novel nitric oxide (NO) responsive monomers (NAPMA and APUEMA) containing o-phenylenediamine functional groups have been polymerized to form NO-responsive macromolecular chains as truly biomimetic polymers. Upon exposure to NO--a ubiquitous cellular signaling molecule--the NAPMA- and APUEMA-labeled thermoresponsive copolymers exhibited substantial changes in solubility, clearly characterized by tuneable LCST behavior, thereby inducing self-assembly into nanoparticulate structures. Moreover, the NO-triggered self-assembly process in combination with environmentally sensitive fluorescence dyes could be employed to detect and image endogenous NO.
Lipidated polymers for the stabilization of cubosomes: nanostructured drug delivery vehicles
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7CC05842J
Abstract: We report a straightforward approach for preparing polymers with lipidic chain ends, and show their application in stabilising nanostructured drug delivery vehicles.
The applications of 3D printing in pulmonary drug delivery and treatment of respiratory disorders
Publisher: Bentham Science Publishers Ltd.
Date: 20-03-2018
DOI: 10.2174/1381612825666181206123414
Abstract: Pulmonary diseases are the third leading cause of morbidity worldwide, however treatment and diagnosis of these diseases continue to be challenging due to the complex anatomical structure as well as physiological processes in the lungs. 3D printing is progressively finding new avenues in the medical field and this technology is constantly being used for diseases where diagnosis and treatment heavily rely on the thorough understanding of complex structural-physiology relationships. The structural and functional complexity of the pulmonary system makes it well suited to 3D printing technology. 3D printing can be used to deconstruct the complex anatomy of the lungs and improve our understanding of its physiological mechanisms, cell interactions and pathophysiology of pulmonary diseases. Thus, this technology can be quite helpful in the discovery of novel therapeutic targets, new drugs and devices for the treatment of lung diseases. The intention of this review is to detail our current understanding of the applications of 3D printing in the design and evaluation of inhalable medicines and to provide an overview on its application in the diagnosis and treatment of pulmonary diseases. This review also discusses other technical and regulatory challenges associated with the progression of 3D printing into clinical practice.
Nano-assemblies of cationic mPEG brush block copolymers with gadolinium polyoxotungstate [Gd(W5O18)(2)](9-) form stable, high relaxivity MRI contrast agents
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8NR00020D
Abstract: Iron-doped silver with lotus-like flower structures as efficient ORR electrocatalysts with outstanding stability and tolerance against various hydrocarbon impurities.
The effect of high-polyphenol extra virgin olive oil on cardiovascular risk factors: A systematic review and meta-analysis.
Publisher: Informa UK Limited
Date: 13-11-2018
Influence of Size and Shape on the Biodistribution of Nanoparticles Prepared by Polymerization-Induced Self-Assembly
Publisher: American Chemical Society (ACS)
Date: 29-09-2017
DOI: 10.1021/ACS.BIOMAC.7B00995
Abstract: Polymerization-induced self-assembly (PISA) is a facile one-pot synthetic technique for preparing polymeric nanoparticles with different sizes and shapes for application in a variety of fields including nanomedicine. However, the in vivo biodistribution of nanoparticles obtained by PISA still remains unclear. To address this knowledge gap, we report the synthesis, cytotoxicity, and biodistribution in an in vivo tumor-bearing mouse model of polystyrene micelles with various sizes and polystyrene filomicelles with different lengths prepared by PISA. First, a library of nanoparticles was prepared comprised of poly(glycidyl methacrylate)-b-poly(oligo(ethylene glycol) methyl ether methacrylate)-b-polystyrene polymers, and their size and morphology were tuned by varying the polystyrene block length without affecting the surface chemistry. The
Lymphatic targeting by albumin-hitchhiking: Applications and optimisation
Publisher: Elsevier BV
Date: 11-2020
The use of endogenous gaseous molecules (NO and CO2) to regulate the self-assembly of a dual-responsive triblock copolymer
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C4PY01678E
Abstract: A nitric oxide (NO) and carbon dioxide (CO 2 ) dual-responsive block copolymer was self-assembled in aqueous solution upon gas stimuli to form nanostructures.
Influence of Monomer Type on Miniemulsion Polymerization Systems Stabilized by Graphene Oxide as Sole Surfactant
Publisher: Wiley
Date: 09-10-2013
DOI: 10.1002/POLA.26947
The impact of size and charge on the pulmonary pharmacokinetics and immunological response of the lungs to PLGA nanoparticles after intratracheal administration to rats
Publisher: Elsevier BV
Date: 11-2020
Modular photo-induced RAFT polymerised hydrogels via thiol-ene click chemistry for 3D cell culturing
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7PY01038A
Abstract: Visible-light induced thiol–ene click gelation of RAFT polymers, creating a modular hydrogel system for 3D cell culture assays.
Utilization of endogenous albumin trafficking pathways in the lungs has potential to modestly increase the lung interstitial access and absorption of drug delivery systems after inhaled administration
Publisher: Informa UK Limited
Date: 03-08-2023
PH-Responsive copolymer micelles to enhance itraconazole efficacy against: Candida albicans biofilms
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/C9TB02586C
Abstract: Candida albicans ( C. albicans ) is a common fungal pathogen causing both localised and systemic infections.
Self‐assembly of well‐defined amphiphilic polymeric miktoarm stars, dendrons, and dendrimers in water: The effect of architecture
Publisher: Wiley
Date: 12-10-2009
DOI: 10.1002/POLA.23672
Synthesis of well-defined poly(acrylates) in ionic liquids via copper(II)-mediated photoinduced living radical polymerization
Publisher: American Chemical Society (ACS)
Date: 27-07-2015
Degradative chain transfer in vinyl acetate polymerizations using toluene as solvent
Publisher: Wiley
Date: 21-06-2007
DOI: 10.1002/POLA.22101
Development of a shape-controlled H2S delivery system using epoxide-functional nanoparticles
Publisher: Wiley
Date: 23-04-2019
DOI: 10.1002/POLA.29382
H2S-Donating trisulfide linkers confer unexpected biological behaviour to poly(ethylene glycol)-cholesteryl conjugates
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/C9TB02614B
Abstract: A comprehensive in vitro study into trisulfide-bearing PEG-conjugates was conducted. For these materials the combination of a cholesteryl group and an H 2 S donating moiety is required to confer cytoprotective and ROS-mitigating effects.
Metal-binding hydrogel particles alleviate soil toxicity and facilitate healthy plant establishment of the native metallophyte grass Astrebla lappacea in mine waste rock and tailings
Publisher: Elsevier BV
Date: 03-2013
DOI: 10.1016/J.JHAZMAT.2013.01.025
Abstract: Soil contaminants are potentially a major threat to human and ecosystem health and sustainable production of food and energy where mineral processing wastes are discharged into the environment. In extreme conditions, metal concentrations in wastes often exceed even the metal tolerance thresholds of metallophytes (metal-tolerant plants) and sites remain barren with high risks of contaminant leaching and dispersion into the environment via erosion. A novel soil amendment based on micron-size thiol functional cross-linked acrylamide polymer hydrogel particles (X3) binds toxic soluble metals irreversibly and significantly reduces their concentrations in the soil solution to below the phytotoxicity thresholds. X3 mixed into the top 50mm of phytotoxic mine waste materials in pots in glasshouse conditions reduced total soluble concentrations of toxic contaminants by 90.3-98.7% in waste rock, and 88.6-96.4% in tailings immediately after application. After 61 days, quality of unamended bottom layer of X3-treated pots was also significantly improved in both wastes. Combination of X3 and metallophytes was more efficient at improving soil solution quality than X3 alone. Addition of X3 to substrates increased substrate water retention and water availability to plants by up to 108% and 98% for waste rock and tailings respectively. Soil quality improvement by X3 allowed successful early establishment of the native metallophyte grass Astrebla lappacea on both wastes where plants failed to establish otherwise.
Magnetic nanoparticles with diblock glycopolymer shells give lectin concentration-dependent MRI signals and selective cell uptake
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C3SC52838C
Phosphorylation of alginate
Publisher: American Chemical Society (ACS)
Date: 07-03-2011
DOI: 10.1021/BM1011773
Abstract: Phosphorylation of alginate was achieved using a heterogeneous urea hosphate reaction. The degree and stereoselectivity of phosphorylation as well as the effects on the physical properties of the polysaccharide were investigated by Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopies, inductively coupled plasma optical-emission spectroscopy (ICP-OES), and size exclusion chromatography (SEC). Multidimensional NMR studies of the phosporylated alginate revealed that phosphorylation of the M residues occurred predominantly at the C3 (equatorial) carbon of the polysaccharide ring. In addition, a more comprehensive assignment of the (1)H NMR spectrum of alginate, compared with those previously reported in the literature, is provided here. Hydrogel materials were formed from ionically cross-linked blends of phosphorylated alginate and alginate. These blended hydrogels showed an enhanced resistance to degradation by chelating agents compared with cross-linked alginate hydrogels and a reduction in their mineralization potential.
Recent advances in magnetic nanoparticle-based molecular probes for hepatocellular carcinoma diagnosis and therapy.
Publisher: Bentham Science Publishers Ltd.
Date: 15-10-2018
DOI: 10.2174/1381612824666180516111939
Abstract: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, leading to the second most likely cause of cancer-related deaths. Medical imaging is crucial in clinic for HCC screening and diagnosis. Due to the relatively high special resolution and excellent sensitivity, magnetic resonance imaging (MRI) by using magnetic nanoparticle-based contrast agents has been used so far in HCC imaging and staging, demonstrating great potential and promising in vivo applications. This review focuses on the use of different magnetic nanoparticles for construction of HCC nanoprobes for MR imaging and theranostic purpose.
Oxytocin receptor antagonists as a novel pharmacological agent for reducing smooth muscle tone in the human prostate
Publisher: Springer Science and Business Media LLC
Date: 18-03-2021
DOI: 10.1038/S41598-021-85439-4
Abstract: Pharmacotherapies for the treatment of Benign Prostatic Hyperplasia (BPH) are targeted at reducing cellular proliferation (static component) or reducing smooth muscle tone (dynamic component), but response is unpredictable and many patients fail to respond. An impediment to identifying novel pharmacotherapies is the incomplete understanding of paracrine signalling. Oxytocin has been highlighted as a potential paracrine mediator of BPH. To better understand oxytocin signalling, we investigated the effects of exogenous oxytocin on both stromal cell proliferation, and inherent spontaneous prostate contractions using primary models derived from human prostate tissue. We show that the Oxytocin Receptor (OXTR) is widely expressed in the human prostate, and co-localises to contractile cells within the prostate stroma. Exogenous oxytocin did not modulate prostatic fibroblast proliferation, but did significantly ( p 0.05) upregulate the frequency of spontaneous contractions in prostate tissue, indicating a role in generating smooth muscle tone. Application of atosiban, an OXTR antagonist, significantly ( p 0.05) reduced spontaneous contractions. In idual tissue responsiveness to both exogenous oxytocin (R 2 = 0.697, p 0.01) and atosiban (R 2 = 0.472, p 0.05) was greater in tissue collected from older men. Overall, our data suggest that oxytocin is a key regulator of inherent spontaneous prostate contractions, and targeting of the OXTR and associated downstream signalling is an attractive prospect in the development of novel BPH pharmacotherapies.
Synthesis of Hollow Polymer Nanocapsules Exploiting Gold Nanoparticles as Sacrificial Templates
Publisher: American Chemical Society (ACS)
Date: 25-01-2010
DOI: 10.1021/MA902663N
Synthesis of monocyclic and linear polystyrene using the reversible coupling/cleavage of thiol/disulfide groups
Publisher: American Chemical Society (ACS)
Date: 12-2006
DOI: 10.1021/MA061070E
Schwann cell endosome CGRP signals elicit periorbital mechanical allodynia in mice
Publisher: Springer Science and Business Media LLC
Date: 03-02-2022
DOI: 10.1038/S41467-022-28204-Z
Abstract: Efficacy of monoclonal antibodies against calcitonin gene-related peptide (CGRP) or its receptor (calcitonin receptor-like receptor/receptor activity modifying protein-1, CLR/RAMP1) implicates peripherally-released CGRP in migraine pain. However, the site and mechanism of CGRP-evoked peripheral pain remain unclear. By cell-selective RAMP1 gene deletion, we reveal that CGRP released from mouse cutaneous trigeminal fibers targets CLR/RAMP1 on surrounding Schwann cells to evoke periorbital mechanical allodynia. CLR/RAMP1 activation in human and mouse Schwann cells generates long-lasting signals from endosomes that evoke cAMP-dependent formation of NO. NO, by gating Schwann cell transient receptor potential ankyrin 1 (TRPA1), releases ROS, which in a feed-forward manner sustain allodynia via nociceptor TRPA1. When encapsulated into nanoparticles that release cargo in acidified endosomes, a CLR/RAMP1 antagonist provides superior inhibition of CGRP signaling and allodynia in mice. Our data suggest that the CGRP-mediated neuronal/Schwann cell pathway mediates allodynia associated with neurogenic inflammation, contributing to the algesic action of CGRP in mice.
Controlled radical polymerization of styrene and methyl acrylate in the presence of reversible addition–fragmentation chain transfer agents, phenylethyl phenyl dithioacetate and phenyldithioacetic aci
Publisher: Wiley
Date: 20-09-2005
DOI: 10.1002/POLA.21020
Synthesis of Thermoresponsive, Catechol-Rich Poly(ethylene glycol) Brush Polymers for Attenuating Cellular Oxidative Stress
Publisher: American Chemical Society (ACS)
Date: 05-12-2022
Biomimetic polymers responsive to a biological signaling molecule: Nitric oxide (NO) triggered reversible self-assembly of single macromolecular chains into nanoparticles
Publisher: Elsevier BV
Date: 09-2015
Microgel stars via Reversible Addition Fragmentation Chain Transfer (RAFT) polymerisation - a facile route to macroporous membranes, honeycomb patterned thin films and inverse opal substrates
Publisher: Royal Society of Chemistry (RSC)
Date: 2003
DOI: 10.1039/B304208C
A tunable one-pot three-component synthesis of an125I and Gd-labelled star polymer nanoparticle for hybrid imaging with MRI and nuclear medicine
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8PY00621K
Abstract: Bimodal radioiodine/Gd labelled polymeric nanoparticles prepared using a versatile one-step three-component click reaction.
A pH-responsive nanoparticle targets the neurokinin 1 receptor in endosomes to prevent chronic pain
Publisher: Springer Science and Business Media LLC
Date: 04-11-2019
PEGylated gold nanoparticles functionalized with β-cyclodextrin inclusion complexes: Towards metal nanoparticle-polymer-carbohydrate cluster biohybrid materials
Publisher: CSIRO Publishing
Date: 2010
DOI: 10.1071/CH10091
Abstract: A cholesterol-functional trithiocarbonate reversible addition–fragmentation chain transfer (RAFT) agent was synthesized and employed to generate well-defined poly(polyethylene glycol) acrylate with cholesterol chain termini using RAFT polymerization. Subsequently, the polymers were grafted onto the surface of gold nanoparticles using the trithiocarbonate functionality to bind to the gold surface. The cholesterol moieties were then modified via complexation with β-cyclodextrin. The step-by-step modification of gold nanoparticles was characterized by dynamic light scattering, attenuated total reflection infrared spectroscopy and surface plasmon resonance analysis.
Glycopolymer Decoration of Gold Nanoparticles Using a LbL Approach
Publisher: American Chemical Society (ACS)
Date: 10-03-2010
DOI: 10.1021/MA100250X
Precise control of drug loading and release of an NSAID–polymer conjugate for long term osteoarthritis intra-articular drug delivery
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7TB01518F
Abstract: The synthesis of biocompatible polymer diclofenac conjugates (PDCs) that have a high drug loading and high degree of control over diclofenac (DCF) release is described.
Adsorption of Well-Defined Fluorine-Containing Polymers onto Poly(tetrafluoroethylene)
Publisher: American Chemical Society (ACS)
Date: 17-10-2008
DOI: 10.1021/LA802300Q
Abstract: Adsorption of well-defined fluorinated polymers onto clinically relevant poly(tetrafluoroethylene) (PTFE) substrates offers an attractive method for modifying the surface properties of chemically inert PTFE. Reversible addition-fragmentation chain transfer (RAFT) was successfully used for synthesis of the polymers in this study: the homopolymers poly(2,3,4,5,6-pentafluorostyrene) (PFS), poly(2,2,3,3-tetrafluoropropyl acrylate) (PTFPA), and poly(2,2,3,3-tetrafluoropropyl methacrylate) (PTFPMA) as well as their block copolymers with tert-butyl acrylate ( (t)BA). Water-soluble blocks were synthesized through the hydrolysis of the t-butyl side groups of P( (t)BA) to the corresponding carboxylic acid. Adsorption of selected polymers onto PTFE from a series of solvents (methyl ethyl ketone (MEK), dimethylformamide (DMF), fluorobenzene (FB), dichloromethane (DCM)) was investigated using X-ray photoelectron spectroscopy (XPS) and sessile water drop measurements. The three homopolymers studied all adsorbed irreversibly (i.e., were not removed by washing) from organic solvents at ambient temperature. PFS displayed the highest adsorption, and was attributed to strong hydrophobic interactions. From angle-resolved XPS it was concluded that PFS became impregnated into the PTFE substrate down to depths of 100 A when using FB as a solvent. The carboxylic acid-containing block copolymers adsorbed more effectively from DMF (a good solvent for the poly(acrylic acid) block) compared to MEK. The resulting modified PTFE substrates displayed high stability with respect to desorption in aqueous solution, yet conformational changes of the adsorbed polymer resulted in a switchable hydrophobic-hydrophilic surface (in air or water, respectively). These results highlight the success of a facile and simple approach to irreversibly adsorb functional polymers to a nonfunctional fluorinated surface.
Copper(0)-mediated radical polymerisation in a self-generating biphasic system
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C2PY20560B
Bioconjugation and Fluorescence Labeling of Iron Oxide Nanoparticles Grafted with Bromomaleimide-Terminal Polymers
Publisher: American Chemical Society (ACS)
Date: 11-10-2018
DOI: 10.1021/ACS.BIOMAC.8B01282
Abstract: Iron oxide nanoparticles have been widely applied in biomedical applications for their unique physical properties. Despite the relatively mature synthetic approaches for iron oxide nanoparticles, surface modification strategies for obtaining particles with satisfactory biofunctionality are still urgently needed to meet the challenge of nanomedicine. Herein, we report a surface modification and biofunctionalization strategy for iron oxide-based magnetic nanoparticles based on a dibromomaleimide (DBM)-terminated polymer with brushed polyethylene glycol (PEG) chains. PEG acrylate and phosphonate monomers, serving as antibiofouling and surface anchoring compartments for iron oxide nanoparticles, were incorporated utilizing a novel DBM containing reversible addition-fragmentation chain transfer (RAFT) agent. The particles prepared through this new surface architecture possessed high colloidal stability in a physiological buffer and the capacity of covalent conjugation with biomolecules for targeting. Cell tracking of the molecular probes was achieved concomitantly by exploiting DBM conjugation-induced fluorescence of the nanoparticles.
Reactive Alkyne and Azide Solid Supports To Increase Purity of Novel Polymeric Stars and Dendrimers via the “Click” Reaction
Publisher: American Chemical Society (ACS)
Date: 10-08-2007
DOI: 10.1021/MA071121N
Nano-sized graphene oxide as sole surfactant in miniemulsion polymerization for nanocomposite synthesis: Effect of pH and ionic strength
Publisher: Elsevier BV
Date: 08-2014
Synthesis and Aggregation Behavior of Four-Arm Star Amphiphilic Block Copolymers in Water
Publisher: American Chemical Society (ACS)
Date: 13-10-2006
DOI: 10.1021/LA0616449
Abstract: We report the first synthesis of hiphilic four-arm star diblock copolymers consisting of styrene (STY) and acrylic acid (AA) made using reversible addition-fragmentation chain transfer (RAFT Z group approach with no star-star coupling). The polymerization proceeded in an ideal "living" manner. The size of the poly(AA(132)-STY(m)4 stars in DMF were small and close to 7 nm, suggesting no star aggregation. Slow addition of water (pH = 6.8) to this mixture resulted in aggregates of 15 stars per micelle with core-shell morphology. Calculations showed that the polyAA blocks were slightly extended with a shell thickness of 15 nm. Treatment of these micelles with piperidine to cleave the block arms from the core resulted in little or no change on micelle size or morphology, but the polyAA shell thickness was close to 29 nm (33 nm is the maximum at full extension) suggesting a release of entropy when the arms are detached from the core molecule. In this work we showed through the use of star hiphilic polymers that the micelle size, aggregation number, and morphology could be controlled.
Outer‐sphere electron transfer metal‐catalyzed polymerization of styrene using a macrobicyclic ligand
Publisher: Wiley
Date: 16-11-2007
DOI: 10.1002/POLA.22366
An optimised Cu(0)-RDRP approach for the synthesis of lipidated oligomeric vinyl azlactone: Toward a versatile antimicrobial materials screening platform
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9TB01624D
Abstract: For the first time Cu(0)-RDRP conditions were optimised to allow for the fast and controlled polymerisation of vinyl azlactone with tuneable lipid elements: a versatile platform material for the high-throughput synthesis of antimicrobial materials.
Water-Soluble, Thermoresponsive, Hyperbranched Copolymers Based on PEG-Methacrylates: Synthesis, Characterization, and LCST Behavior
Publisher: Wiley
Date: 24-05-2010
DOI: 10.1002/POLA.24027
Surface-Functionalized Polymer Nanoparticles for Selective Sequestering of Heavy Metals
Publisher: Wiley
Date: 03-03-2006
Cellular Uptake of Densely Packed Polymer Coatings on Gold Nanoparticles
Publisher: American Chemical Society (ACS)
Date: 30-11-2009
DOI: 10.1021/NN9011237
Abstract: A variety of functional polymer chains prepared by RAFT were directly grafted onto 5, 10, and 20 nm gold nanoparticles (AuNPs). The polymer shell coating the AuNPs was densely packed because of the strong binding between the trithioester groups on the polymer chain-ends and gold. It was found that due to the densely packed nature of the shell the polymer chains were significantly stretched compared to their usual Gaussian coil conformation in water. This was even evident for polymer chains where ionic repulsion between neighboring chains should be significant. Therefore, with such high grafting densities the surface properties and size of the hybrid nanoparticles should be the only contributing factors in cellular uptake in epithelial Caco-2 cells. This study has provided valuable insight into the effects of charge and size of NPs for the application of NPs in the delivery of therapeutic agents across the intestine. Our results showed that the negatively charged AuNPs were taken up by the cells with greater efficiency than the neutral AuNPs, most probably due to binding with membrane proteins. The positively charged AuNPs as expected gave the greatest uptake efficiency. Interestingly, the uptake for PNIPAM-AuNPs (hydrophobic coating at 37 degrees C) increased from approximately 2% efficiency after a 30 min incubation to 8% after 2 h, and was much greater than the negative or neutral AuNPs. We believe that this was due to the interplay between the hydrophobic nature of the NPs and their increased size.
Cationic acrylate oligomers comprising amino acid mimic moieties demonstrate improved antibacterial killing efficiency
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C6TB02787C
Abstract: A new class of oligomeric cationic polymers with lipophilic tails were developed as antibacterial lipopeptide mimics, and revealed structurally dependent bacterial killing.
Organic arsenicals as efficient and highly specific linkers for protein/peptide-polymer conjugation
Publisher: American Chemical Society (ACS)
Date: 20-03-2015
DOI: 10.1021/JACS.5B01140
Abstract: The entropy-driven affinity of trivalent (in)organic arsenicals for closely spaced dithiols has been exploited to develop a novel route to peptide rotein-polymer conjugation. A trivalent arsenous acid (As(III)) derivative (1) obtained from p-arsanilic acid (As(V)) was shown to readily undergo conjugation to the therapeutic peptide salmon calcitonin (sCT) via bridging of the Cys(1)-Cys(7) disulfide, which was verified by RP-HPLC and MALDI-ToF-MS. Conjugation was shown to proceed rapidly (t < 2 min) in situ and stoichiometrically through sequential reduction-conjugation protocols, therefore exhibiting conjugation efficiencies equivalent to those reported for the current leading disulfide-bond targeting strategies. Furthermore, using bovine serum albumin as a model protein, the trivalent organic arsenical 1 was found to demonstrate enhanced specificity for disulfide-bond bridging in the presence of free cysteine residues relative to established maleimide functional reagents. This specificity represents a shift toward potential orthogonality, by clearly distinguishing between the reactivity of mono- and disulfide-derived (vicinal or neighbors-through-space) dithiols. Finally, p-arsanilic acid was transformed into an initiator for aqueous single electron-transfer living radical polymerization, allowing the synthesis of hydrophilic arsenic-functional polymers which were shown to exhibit negligible cytotoxicity relative to a small molecule organic arsenical, and an unfunctionalized polymer control. Poly(poly[ethylene glycol] methyl ether acrylate) (PPEGA480, DPn = 10, Mn,NMR = 4900 g·mol(-1), Đ = 1.07) possessing a pentavalent arsenic acid (As(V)) α-chain end was transformed into trivalent As(III) post-polymerization via initial reduction by biological reducing agent glutathione (GSH), followed by binding of GSH. Conjugation of the resulting As(III)-functional polymer to sCT was realized within 35 min as indicated by RP-HPLC and verified later by thermodynamically driven release of sCT, from the conjugate, in the presence of strong chelating reagent ethanedithiol.
Star Polymers Reduce Islet Amyloid Polypeptide Toxicity via Accelerated Amyloid Aggregation
Publisher: American Chemical Society (ACS)
Date: 31-10-2017
One-Pot Sequence-Controlled (SC) Multiblock Copolymers via Copper-Mediated Polymerization
Publisher: Wiley
Date: 04-12-2018
Exploiting Macromolecular Design to Optimize the Antibacterial Activity of Alkylated Cationic Oligomers
Publisher: American Chemical Society (ACS)
Date: 25-10-2018
DOI: 10.1021/ACS.BIOMAC.8B01317
Abstract: There is growing interest in synthetic polymers which co-opt the structural features of naturally occurring antimicrobial peptides. However, our understanding of how macromolecular architecture affects antibacterial activity remains limited. To address this, we investigated whether varying architectures of a series of block and statistical co-oligomers influenced antibacterial and hemolytic activity. Cu(0)-mediated polymerization was used to synthesize oligomers constituting 2-(Boc-amino)ethyl acrylate units and either diethylene glycol ethyl ether acrylate (DEGEEA) or poly(ethylene glycol) methyl ether acrylate units with varying macromolecular architecture subsequent deprotection produced primary amine functional oligomers. Further guanylation provided an additional series of antimicrobial candidates. Both chemical composition and macromolecular architecture were shown to affect antimicrobial activity. A broad spectrum antibacterial oligomer (containing guanidine moieties and DEGEEA units) was identified that possessed promising activity (MIC = 2 μg mL
An overview of protein–polymer particles
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C0SM00412J
Physiological and pharmacological impact of oxytocin on epididymal propulsion during the ejaculatory process in rodents and men
Publisher: Wiley
Date: 27-05-2021
Thiol-Reactive Star Polymers Display Enhanced Association with Distinct Human Blood Components
Publisher: American Chemical Society (ACS)
Date: 03-04-2017
Abstract: Directing nanoparticles to specific cell types using nonantibody-based methods is of increasing interest. Thiol-reactive nanoparticles can enhance the efficiency of cargo delivery into specific cells through interactions with cell-surface proteins. However, studies to date using this technique have been largely limited to immortalized cell lines or rodents, and the utility of this technology on primary human cells is unknown. Herein, we used RAFT polymerization to prepare pyridyl disulfide (PDS)-functionalized star polymers with a methoxy-poly(ethylene glycol) brush corona and a fluorescently labeled cross-linked core using an arm-first method. PDS star polymers were examined for their interaction with primary human blood components: six separate white blood cell subsets, as well as red blood cells and platelets. Compared with control star polymers, thiol-reactive nanoparticles displayed enhanced association with white blood cells at 37 °C, particularly the phagocytic monocyte, granulocyte, and dendritic cell subsets. Platelets associated with more PDS than control nanoparticles at both 37 °C and on ice, but they were not activated in the duration examined. Association with red blood cells was minor but still enhanced with PDS nanoparticles. Thiol-reactive nanoparticles represent a useful strategy to target primary human immune cell subsets for improved nanoparticle delivery.
Reference Gene U2 Enables Direct Comparison between Relative Gene Expression Levels of Vascular Smooth Muscle Cells in Tissue and Culture Using Real-Time Quantitative PCR
Publisher: MDPI AG
Date: 23-08-2023
Abstract: In nearly every lab, real-time quantitative polymerase chain reaction (qPCR) is used to quantify gene expression. However, a comparison of different s les requires the careful selection of suitable reference genes (RGs), sometimes referred to as housekeeping genes. In the case of vascular smooth muscle cells (vSMCs), it is important to know under which conditions gene expression in isolated and cultured vSMCs can be compared with vSMCs in a healthy blood vessel. We isolated the vSMC-containing layer of the rat aorta (tunica media) and used one (longitudinal) half for direct RNA extraction, while the other half served to isolate and culture vSMCs prior to RNA extraction. First, the expression of the routinely used RGs beta-actin (Actb) and Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) is investigated in intact media and corresponding cultured vSMCs. Significant differences in their Ct values show that these RGs could not be used for such direct comparisons therefore, we select 15 different RGs. Only the gene expression of the small ribonuclear protein (snRNP) U2 shows no significant differences between the absolute Ct values of cultured vSMCs and the intact media moreover, no differences were found between male and female rats in our experimental setup. In conclusion, U2 was shown to be an appropriate (sex-independent) RG to compare relative expression levels of vSMCs in culture to those vSMCs within their physiological tissue environment.
The importance of nanoparticle shape in cancer drug delivery
Publisher: Informa UK Limited
Date: 20-08-2015
DOI: 10.1517/17425247.2014.950564
Abstract: Nanoparticles have been successfully used for cancer drug delivery since 1995. In the design of commercial nanoparticles, size and surface characteristics have been exploited to achieve efficacious delivery. However, the design of optimized drug delivery platforms for efficient delivery to disease sites with minimal off-target effects remains a major research goal. One crucial element of nanoparticle design influencing both pharmacokinetics and cell uptake is nanoparticle morphology (both size and shape). In this succinct review, the authors collate the recent literature to assess the current state of understanding of the influence of nanoparticle shape on the effectiveness of drug delivery with a special emphasis on cancer therapy. This review draws on studies that have focused on the role of nonspherical nanoparticles used for cancer drug delivery. In particular, the authors summarize the influence of nanoparticle shape on biocirculation, biodistribution, cellular uptake and overall drug efficacy. By comparing spherical and nonspherical nanoparticles, they establish some general design principles to serve as guidelines for developing the next generation of nanocarriers for drug delivery. Pioneering studies on nanoparticles show that nonspherical shapes show great promise as cancer drug delivery vectors. Filamentous or worm-like micelles together with other rare morphologies such as needles or disks may become the norm for next-generation drug carriers, though at present, traditional spherical micelles remain the dominant shape of nanocarriers described in the literature due to synthesis and testing difficulties. The few reports that do exist describing nonspherical nanoparticles show a number of favorable properties that should encourage more efforts to develop facile and versatile nanoparticle synthesis methodologies with the flexibility to create different shapes, tunable sizes and adaptable surface chemistries. In addition, the authors note that there is a current lack of understanding into the factors governing (and optimizing) the inter-relationships of size, surface characteristics and shapes of many nanoparticles proposed for use in cancer therapy.
Divergent synthesis and self‐assembly of amphiphilic polymeric dendrons with selective degradable linkages
Publisher: Wiley
Date: 22-01-2008
DOI: 10.1002/POLA.22528
Polymers with Dithiobenzoate End Groups Constitutively Release Hydrogen Sulfide upon Exposure to Cysteine and Homocysteine
Publisher: American Chemical Society (ACS)
Date: 26-03-2020
Optimising dissolved air flotation/filtration treatment of algae-laden lagoon effluent using surface charge: A Bolivar treatment plant case study
Publisher: IWA Publishing
Date: 10-2012
DOI: 10.2166/WST.2012.373
Abstract: Dissolved air flotation (DAF) incorporating filtration (DAFF) is used at the Bolivar wastewater treatment plant (WWTP) to polish lagoon effluent for reuse. Elevated algal populations are frequently experienced and can lead to increased coagulant requirements and process control issues. Streaming current detectors (SCDs) and a charge demand analyser (CDA) were used to monitor the full-scale plant. This was followed by an optimisation study using a pilot plant with a CDA. It was found that the normal operational charge demand range for DAF at Bolivar was between −46 and −40 μeq L−1. Decreasing the pH of coagulation reduced coagulant consumption and facilitated more sensitive CDA responses to changes in alum dose.
Facile access to thermoresponsive filomicelles with tuneable cores
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6CC00900J
Abstract: Thermoresponsive nanoworms with tuneable cores prepared via aqueous RAFT-mediated emulsion polymerizations and temperature-induced morphological transformation (TIMT) technique.
Hydrolyzable Poly[Poly(Ethylene Glycol) Methyl Ether Acrylate]-Colistin Prodrugs through Copper-Mediated Photoinduced Living Radical Polymerization
Publisher: American Chemical Society (ACS)
Date: 07-07-2017
Synthesis of 3-Miktoarm Stars and 1st Generation Mikto Dendritic Copolymers by “Living” Radical Polymerization and “Click” Chemistry
Publisher: American Chemical Society (ACS)
Date: 12-08-2006
DOI: 10.1021/JA0645990
Abstract: The facile synthesis of 3-miktoarm star polymers and 1st generation mikto polymeric dendrimers using atom transfer radical polymerization (ATRP) and "click" chemistry is demonstrated. ATRP was used to synthesize near uniform polymers with Br chain ends, which were easily converted into azido groups. These polymer chains were then attached to a trifunctional alkyne molecule (tripropargylamine) using click reactions in a variety of ways to make the miktoarm stars and miktoarm polymeric dendrimers.
Antibacterial low molecular weight cationic polymers: dissecting the contribution of hydrophobicity, chain length and charge to activity
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C5RA24361K
Abstract: We report the antibacterial activity of a novel class of low molecular weight cationic polymers synthesised using Cu(0) mediated polymerisation.
Facile production of nanoaggregates with tuneable morphologies from thermoresponsive P(DEGMA-co-HPMA)
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C5PY01467K
Abstract: RAFT-mediated emulsion polymerization of styrene and subsequent morphological transition produces nanoaggregates with tuneable morphologies.
Synthesis and postfunctionalization of well-defined star polymers via double click chemistry
Publisher: Wiley
Date: 04-10-2011
DOI: 10.1002/POLA.25001
Design and Synthesis of Dual Thermoresponsive and Antifouling Hybrid Polymer/Gold Nanoparticles
Publisher: American Chemical Society (ACS)
Date: 10-07-2009
DOI: 10.1021/MA9013127
Stability Enhancing N-Terminal PEGylation of Oxytocin Exploiting Different Polymer Architectures and Conjugation Approaches
Publisher: American Chemical Society (ACS)
Date: 15-07-2016
DOI: 10.1021/ACS.BIOMAC.6B00919
Abstract: Oxytocin, a cyclic nine amino acid neurohypophyseal hormone therapeutic, is effectively used in the control of postpartum hemorrhaging (PPH) and is on the WHO List of Essential Medicines. However, oxytocin has limited shelf life stability in aqueous solutions, particularly at temperatures in excess of 25 °C and injectable aqueous oxytocin formulations require refrigeration (<8 °C). This is particularly problematic in the hot climates often found in many developing countries where daytime temperatures can exceed 40 °C and where reliable cold-chain storage is not always achievable. The purpose of this study was to develop N-terminal amine targeted PEGylation strategies utilizing both linear PEG and polyPEG "comb" polymers as an effective method for stabilizing solution formulations of this peptide for prolonged storage in the absence of efficient cold-chain storage. The conjugation chemistries investigated herein include irreversible amine targeted conjugation methods utilizing NHS ester and aldehyde reductive amination chemistry. Additionally, one reversible conjugation method using a Schiff base approach was explored to allow for the release of the native peptide, thus, ensuring that biological activity remains unaffected. The reversibility of this approach was investigated for the different polymer architectures, alongside a nonpolymer oxytocin analogue to monitor how pH can tune native peptide release. Elevated temperature degradation studies of the polymer conjugates were evaluated to assess the stability of the PEGylated analogues in comparison to the native peptide in aqueous formulations to mimic storage conditions in developing nations and regions where storage under appropriate conditions is challenging.
Metal-binding particles alleviate lead and zinc toxicity during seed germination of metallophyte grass Astrebla lappacea
Publisher: Elsevier BV
Date: 06-2011
DOI: 10.1016/J.JHAZMAT.2011.03.116
Abstract: Combining metal-binding particles and metal-tolerant plants (metallophytes) offers a promising new approach for rehabilitation of heavy metal contaminated sites. Three types of hydrogel metal-binding polymer particles were synthesized and their effects on metal concentrations tested in vitro using metal ion solutions. The most effective of the tested polymers was a micron-sized thiol functional cross-linked acrylamide polymer which reduced the available solution concentrations of Pb(2+) (9.65 mM), Cu(2+) (4mM) and Zn(2+) (10mM) by 86.5%, 75.5% and 63.8%, respectively, and was able to store water up to 608% of its dry mass. This polymer was not toxic to seed germination. In deionised water, it enhanced seed germination, and at otherwise phytotoxic Pb(2+) (9.65 mM) and Zn(2+) (10mM) concentrations, it allowed normal germination and root elongation of the metallophyte grass Astrebla lappacea. We conclude that the polymer has the potential to facilitate restoration of heavy metal contaminated lands by reducing the concentration of metal cations in the soil solution and improving germination rates through reduced toxicity and enhanced plant water relations.
Micron-size metal-binding hydrogel particles improve germination and radicle elongation of Australian metallophyte grasses in mine waste rock and tailings
Publisher: Elsevier BV
Date: 03-2013
DOI: 10.1016/J.JHAZMAT.2013.01.049
Abstract: Metal contamination of landscapes as a result of mining and other industrial activities is a pervasive problem worldwide. Metal contaminated soils often lack effective vegetation cover and are prone to contaminant leaching and dispersion through erosion, leading to contamination of the environment. Metal-binding hydrogel particle amendments could ameliorate mine wastes prior to planting and enhance seedling emergence. In this study, micron-size thiol functional cross-linked acrylamide polymer hydrogel particles (X3) were synthesised and tested in laboratory-scale experiments on phytotoxic mine wastes to determine their capacity to: (i) increase substrate water holding capacity (WHC) (ii) reduce metal availability to plants to below the phytotoxicity threshold and (iii) enhance germination characteristics and early radicle development of two Australian metallophyte grasses under limiting and non-limiting water conditions. Addition of X3 to mine wastes significantly increased their WHC and lowered toxic soluble metal concentrations in mine waste leachates. Germination percentages and radicle elongation of both grasses in wastes were significantly increased. Highest germination percentages and greater radicle development recorded in X3 amended wastes under water limited conditions suggests that X3 was able to ameliorate metal toxicity to radicles, and provide moisture, which improved the imbibition and consequent germination of the seeds.
Surfactant-free RAFT emulsion polymerization using a novel biocompatible thermoresponsive polymer
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C6PY02158A
Abstract: A facile, high-scale, and versatile technique to prepare biocompatible nanoparticles with tailorable properties from thermoresponsive macro-CTAs and macro-stabilizers.
A HIGH-RESOLUTION NMR INVESTIGATION INTO THE MICROSTRUCTURE OF HEMA AND EEMA COPOLYMERS
Publisher: Elsevier BV
Date: 1995
A traceless reversible polymeric colistin prodrug to combat multidrug-resistant (MDR) gram-negative bacteria.
Publisher: Elsevier BV
Date: 08-2017
DOI: 10.1016/J.JCONREL.2017.02.005
Abstract: Colistin methanesulfonate (CMS) is the only prodrug of colistin available for clinical use for the treatment of infections caused by multidrug-resistant (MDR) Gram-negative bacteria. Owing to its slow and variable release, an alternative is urgently required to improve effectiveness. Herein we describe a PEGylated colistin prodrug whereby the PEG is attached via a cleavable linker (col-aaPEG) introducing an acetic acid terminated poly (ethylene glycol) methyl ether (aaPEG) onto the Thr residue of colistin. Due to the labile ester containing link, this prodrug is converted back into active colistin in vitro within 24h. Compared to CMS, it showed a similar or better antimicrobial performance against two MDR isolates of Pseudomonas aeruginosa and Acinetobacter baumannii through in vitro disk diffusion, broth dilution and time-kill studies. In a mouse infection model, col-aaPEG displayed acceptable bacterial killing against P. aeruginosa ATCC 27853 and no nephrotoxicity was found after systemic administration, suggesting it to be a potential alternative for CMS.
Intra-articular Treatment of Osteoarthritis with Diclofenac-Conjugated Polymer Reduces Inflammation and Pain
Publisher: American Chemical Society (ACS)
Date: 24-05-2019
Abstract: The most common treatment for osteoarthritis is daily oral administration of a nonsteroidal anti-inflammatory drug such as diclofenac. This daily dosage regime is often associated with severe side effects. In this study, we explored the potential of utilizing a high molecular weight cross-linked polyurethane polymer covalently linked to diclofenac (
Highly-ordered hybrid organic-inorganic isoporous membranes from polymer modified nanoparticles
Publisher: Wiley
Date: 30-03-2005
Recent advances in the delivery of hydrogen sulfide via a macromolecular approach
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8PY00938D
Abstract: This mini review highlights recent advances in the design of macromolecular materials that can deliver hydrogen sulfide either spontaneously or in response to chemical and physical triggers.
Functional, star polymeric molecular carriers, built from biodegradable microgel/nanogel cores
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C0CC04532B
Abstract: Acid and disulfide biodegradable cross-linkers have been employed to generate microgel star polymers, using RAFT-polymer arms. RAFT end-groups were then exploited to attach functional compounds via both thiol-ene and thiol-pyridyl disulfide exchange reactions.
Delivering nitric oxide with nanoparticles
Publisher: Elsevier BV
Date: 05-2015
DOI: 10.1016/J.JCONREL.2015.02.007
Abstract: While best known for its important signalling functions in human physiology, nitric oxide is also of considerable therapeutic interest. As such, nanoparticle-based systems which enable the sustained exogenous delivery of nitric oxide have been the subject of considerable investigation in recent years. Herein we review the various nanoparticle systems that have been used to date for nitric oxide delivery, and explore the array of potential therapeutic applications that have been reported. Specifically, we discuss the modification of sol-gel based silica particles, functionalised metal/metal oxide nanoparticles, polymer-coated metal nanoparticles, dendrimers, micelles and star polymers to impart nitric oxide release capability. We also consider the various areas in which therapeutic applications are envisaged: wound healing, antimicrobial applications, cardiovascular treatments, sexual medicine and cancer treatment. Finally, we discuss possible future directions for this versatile and potentially important technology.
Macromolecular Hydrogen Sulfide Donors Trigger Spatiotemporally Confined Changes in Cell Signaling
Publisher: American Chemical Society (ACS)
Date: 23-12-2016
DOI: 10.1021/ACS.BIOMAC.5B01469
Abstract: Hydrogen sulfide (H2S) is involved in a myriad of cell signaling processes that trigger physiological events ranging from vasodilation to cell proliferation. Moreover, disturbances to H2S signaling have been associated with numerous pathologies. As such, the ability to release H2S in a cellular environment and stimulate signaling events is of considerable interest. Herein we report the synthesis of macromolecular H2S donors capable of stimulating cell signaling pathways in both the cytosol and at the cell membrane. Specifically, copolymers having pendent oligo(ethylene glycol) and benzonitrile groups were synthesized, and the benzonitrile groups were subsequently transformed into primary aryl thioamide groups via thionation using sodium hydrosulfide. These thioamide moieties could be incorporated into a hydrophilic copolymer or a block copolymer (i.e., into either the hydrophilic or hydrophobic domain). An electrochemical sensor was used to demonstrate release of H2S under simulated physiological conditions. Subsequent treatment of HEK293 cells with a macromolecular H2S donor elicited a slow and sustained increase in cytosolic ERK signaling, as monitored using a FRET-based biosensor. The macromolecular donor was also shown to induce a small, fast and sustained increase in plasma membrane-localized PKC activity immediately following addition to cells. Studies using an H2S-selective fluorescent probe in live cells confirmed release of H2S from the macromolecular donor over physiologically relevant time scales consistent with the signaling observations. Taken together, these results demonstrate that by using macromolecular H2S donors it is possible to trigger spatiotemporally confined cell signaling events. Moreover, the localized nature of the observed signaling suggests that macromolecular donor design may provide an approach for selectively stimulating certain cellular biochemical pathways.
Hydrophobically-associating cationic polymers as micro-bubble surface modifiers in dissolved air flotation for cyanobacteria cell separation
Publisher: Elsevier BV
Date: 09-2014
DOI: 10.1016/J.WATRES.2014.05.032
Abstract: Dissolved air flotation (DAF), an effective treatment method for clarifying algae/cyanobacteria-laden water, is highly dependent on coagulation-flocculation. Treatment of algae can be problematic due to unpredictable coagulant demand during blooms. To eliminate the need for coagulation-flocculation, the use of commercial polymers or surfactants to alter bubble charge in DAF has shown potential, termed the PosiDAF process. When using surfactants, poor removal was obtained but good bubble adherence was observed. Conversely, when using polymers, effective cell removal was obtained, attributed to polymer bridging, but polymers did not adhere well to the bubble surface, resulting in a cationic clarified effluent that was indicative of high polymer concentrations. In order to combine the attributes of both polymers (bridging ability) and surfactants (hydrophobicity), in this study, a commercially-available cationic polymer, poly(dimethylaminoethyl methacrylate) (polyDMAEMA), was functionalised with hydrophobic pendant groups of various carbon chain lengths to improve adherence of polymer to a bubble surface. Its performance in PosiDAF was contrasted against commercially-available poly(diallyl dimethyl ammonium chloride) (polyDADMAC). All synthesised polymers used for bubble surface modification were found to produce positively charged bubbles. When applying these cationic micro-bubbles in PosiDAF, in the absence of coagulation-flocculation, cell removals in excess of 90% were obtained, reaching a maximum of 99% cell removal and thus demonstrating process viability. Of the synthesised polymers, the polymer containing the largest hydrophobic functionality resulted in highly anionic treated effluent, suggesting stronger adherence of polymers to bubble surfaces and reduced residual polymer concentrations.
Elucidating the effect of sequence and degree of polymerization on antimicrobial properties for block copolymers
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9PY01435G
Abstract: Sequence-controlled copolymers have recently attracted great interest in a variety of applications, including antimicrobial materials.
Uptake and transcytosis of functionalized superparamagnetic iron oxide nanoparticles in an in vitro blood brain barrier model
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C7BM01012E
Abstract: Phosphoryl choline grafting secured high cellular uptake and increased transcytosis of superparamagnetic iron oxide nanoparticles through a model blood brain barrier.
Synthesis of multi-block copolymer stars using a simple iterative Cu(0)-mediated radical polymerization technique
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C1PY00384D
Abstract: A new iterative copper(0)-mediated radical polymerization approach is presented that represents a significant advance in the synthesis of high order multi-block star copolymers. The synthesis of these materials can now be achieved in high yield and with controlled structural complexity, with purification only required at the last step. The approach is general, facile and offers the opportunity to synthesize new copolymer stars.
Optimizing the generation of narrow polydispersity ‘arm-first’ star polymers made using RAFT polymerization
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C1PY00102G
Functional iron oxide magnetic nanoparticles with hyperthermia-induced drug release ability by using a combination of orthogonal click reactions
Publisher: Wiley
Date: 19-11-2013
Effect of Degassing on Surfactant-Free Emulsion Polymerizations of Styrene Mediated with RAFT
Publisher: American Chemical Society (ACS)
Date: 07-01-2006
DOI: 10.1021/MA052295C
Elucidating the Influences of Size, Surface Chemistry, and Dynamic Flow on Cellular Association of Nanoparticles Made by Polymerization-Induced Self-Assembly
Publisher: Wiley
Date: 25-07-2018
Abstract: The size and surface chemistry of nanoparticles dictate their interactions with biological systems. However, it remains unclear how these key physicochemical properties affect the cellular association of nanoparticles under dynamic flow conditions encountered in human vascular networks. Here, the facile synthesis of novel fluorescent nanoparticles with tunable sizes and surface chemistries and their association with primary human umbilical vein endothelial cells (HUVECs) is reported. First, a one-pot polymerization-induced self-assembly (PISA) methodology is developed to covalently incorporate a commercially available fluorescent dye into the nanoparticle core and tune nanoparticle size and surface chemistry. To characterize cellular association under flow, HUVECs are cultured onto the surface of a synthetic microvascular network embedded in a microfluidic device (SynVivo, INC). Interestingly, increasing the size of carboxylic acid-functionalized nanoparticles leads to higher cellular association under static conditions but lower cellular association under flow conditions, whereas increasing the size of tertiary amine-decorated nanoparticles results in a higher level of cellular association, under both static and flow conditions. These findings provide new insights into the interactions between polymeric nanomaterials and endothelial cells. Altogether, this work establishes innovative methods for the facile synthesis and biological characterization of polymeric nanomaterials for various potential applications.
Nitric oxide-sensing actuators for modulating structure in lipid-based liquid crystalline drug delivery systems
Publisher: Elsevier BV
Date: 12-2017
DOI: 10.1016/J.JCIS.2017.08.079
Abstract: Herein we report on the development of a nitric oxide-sensing lipid-based liquid crystalline (LLC) system specifically designed to release encapsulated drugs on exposure to NO through a stimulated phase change. A series of nitric oxide (NO)-sensing lipids compatible with phytantriol and GMO cubic phases were designed and synthesized, and utilized in enabling nitric oxide-sensing LLC systems. The nitric oxide (NO)-sensing lipids react with nitric oxide, resulting in hydrolysis of these lipids and phase transition of the LLC system. Specifically, the N-3-aminopyridinyl myristylamine (NAPyM)+phytantriol mixture formed a lamellar phase in excess aqueous environment. The NAPyM+phytantriol LLC responded to the nitric oxide gas as a chemical stimulus which triggers a phase transition from lamellar phase to inverse cubic and hexagonal phase. The nitric oxide-triggered phase transition of the LLC accelerated the release of encapsulated model drug from the LLC bulk phase, resulting in a 15-fold increase in the diffusion coefficient compared to the starting lamellar structure. The nitric oxide-sensing LLC system has potential application in the development of smart medicines to treat nitric oxide implicated diseases.
Trisulfide-Bearing PEG Brush Polymers Donate Hydrogen Sulfide and Ameliorate Cellular Oxidative Stress
Publisher: American Chemical Society (ACS)
Date: 19-11-2020
Synthesis of polystyrene nanoparticles "armoured" with nanodimensional graphene oxide sheets by miniemulsion polymerization
Publisher: Wiley
Date: 14-09-2013
DOI: 10.1002/POLA.26341
Photoinduced sequence-control via one pot living radical polymerization of acrylates
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4SC01374C
Abstract: A versatile and inexpensive photochemical platform for the preparation of high-order multiblock functional materials.
Garlic-inspired trisulfide linkers for thiol-stimulated H2S release
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7CC03820H
Abstract: Garlic-inspired cholesterol-mPEG conjugates incorporating a trisulfide linkage have the ability to cleave upon exposure to thiols with a concomitant release of H 2 S.
Transformation of RAFT Polymer End Groups into Nitric Oxide Donor Moieties: En Route to Biochemically Active Nanostructures
Publisher: American Chemical Society (ACS)
Date: 03-11-2015
Trisulfide linked cholesteryl PEG conjugate attenuates intracellular ROS and collagen-1 production in a breast cancer co-culture model
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D0BM01544J
Abstract: Treatment of cancer cell-fibroblast co-cultures with H 2 S-releasing trisulfide conjugate causes restoration of ROS levels in the cancer cells and normalisation of collagen-1 expression in the fibroblasts.
Modulation of the Surface Charge on Polymer-Stabilized Gold Nanoparticles by the Application of an External Stimulus
Publisher: American Chemical Society (ACS)
Date: 06-11-2021
DOI: 10.1021/LA902746V
Abstract: A new approach to controlling the charge on gold nanoparticle surfaces is described. The method exploits the simultaneous coattachment of both charged and neutral polymers onto gold surfaces. The charged and neutral polymers were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, and the RAFT end-group functionality was used as the anchor for attachment to gold. The approach described is general and can be applied to a wide range of monomers those exemplified in the paper are poly(2-aminoethyl methacrylamide) (P(AEA)), poly(acrylic acid) (PAA), and poly(N,N-diemthylaminoethyl acrylate) (P(DMAEA)) together with neutral polymers based on poly(oligoethylene oxide) acrylate (P(OEG-A)). The hybrid polymer-stabilized GNPs thus formed were characterized in solution using dynamic light scattering and zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, X-ray photoelectron spectroscopy, and attenuated total reflection-Fourier-transform IR spectroscopy. The grafting densities of the polymers on GNPs were measured using thermal gravimetric analyses (TGA), as 0.4 chains/nm(2) (for PAA), 0.9 chains/nm(2) (for neutral polymers, such as P(NIPAAm), and 0.6 chain/nm(2) for the positive charged polymers P(AEA) and P(DMAEA). The directed coassembly of two different polymers (one charged and one noncharged) on the gold nanoparticle surfaces provided a mechanism (dependent on molecular weight) for shielding the surface charge imparted by the charged polymer component, allowing for a range of surface charges on the GNPs from -30 to +39 mV. In further work, the surface-charges were modulated by an external stimulus (temperature). The charge-modulation was controlled by the use of thermosensitive neutral polymers coassembled with charged polymers. The thermosensitive polymers exemplified in this paper are P(oligoethylene oxide acrylate-co-diethylene oxide acrylate) (P(OEG-A-co-DEG-A)) and P(N-isopropyl acrylamide) (P(NIPAAm). The temperature of the aqueous phase (from 15 to 70 degrees C) was then adjusted to tune the zeta potentials of the hybrid GNPs from +39 or -30 to approximately 0 mV. Finally, by manipulating the solution pH, reversible aggregation behavior of the hybrid GNPs could be induced.
Nitroxide-functional PEGylated nanostars arrest cellular oxidative stress and exhibit preferential accumulation in co-cultured breast cancer cells
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1TB00812A
Abstract: Conjugation to nanostars enhances the biological performance of TEMPO.
Synthesis of linear and 4‐arm star block copolymers of poly(methyl acrylate‐b‐solketal acrylate) by SET‐LRP at 25 °C
Publisher: Wiley
Date: 13-08-2008
DOI: 10.1002/POLA.22946
High molecular weight block copolymers by sequential monomer addition via Cu(0)-mediated living radical polymerization (SET-LRP): An optimized approach
Publisher: American Chemical Society (ACS)
Date: 23-09-2013
DOI: 10.1021/MZ4004198
Abstract: The synthesis of well-defined high molecular weight block copolymers by sequential in situ chain extensions via Cu(0)-mediated living radical polymerization is reported. Optimal conditions for iterative high molecular weight block formation were determined using model homopolymer quasiblock systems, including methyl acrylate (MA), ethyl acrylate (EA), and
Rapid synthesis of ultrahigh molecular weight and low polydispersity polystyrene diblock copolymers by RAFT-mediated emulsion polymerization
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5PY00166H
Abstract: An environmentally friendly emulsion technique produces uniform nanoparticles with precise control over molecular weight and particle size.
Synthesis and in vitro properties of iron oxide nanoparticles grafted with brushed phosphorylcholine and polyethylene glycol
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C5PY02024G
Abstract: A new and facile strategy for grafting IONPs by phosphonic acic terminated PC brushes has been demonstrated and characterized in vitro .
Synthesis of Soluble Phosphate Polymers by RAFT and Their in Vitro Mineralization.
Publisher: American Chemical Society (ACS)
Date: 17-10-2006
DOI: 10.1021/BM060583Q
Abstract: Soluble linear (non-cross-linked) poly(monoacryloxyethyl phosphate) (PMAEP) and poly(2-(methacryloyloxy)ethyl phosphate) (PMOEP) were successfully synthesized through reversible addition-fragmentation chain transfer (RAFT)-mediated polymerization and by keeping the molecular weight below 20 K. Above this molecular weight, insoluble (cross-linked) polymers were observed, postulated to be due to residual diene (cross-linkable) monomers formed during purification of the monomers, MOEP and MAEP. Block copolymers consisting of PMAEP or PMOEP and poly(2-(acetoacetoxy)ethyl methacrylate) (PAAEMA) were successfully prepared and were immobilized on aminated slides. Simulated body fluid studies revealed that calcium phosphate (CaP) minerals formed on both the soluble polymers and the cross-linked gels were very similar. Both the PMAEP polymers and the PMOEP gel showed a CaP layer most probably brushite or monetite based on the Ca/P ratios. A secondary CaP mineral growth with a typical hydroxyapatite (HAP) globular morphology was found on the PMOEP gel. The soluble PMOEP film formed carbonated HAP according to Fourier transform infrared (FTIR) spectroscopy. Block copolymers attached to aminated slides showed only patchy mineralization, possibly due to the ionic interaction of negatively charged phosphate groups and protonated amines.
Nitric oxide (NO) cleavable biomimetic thermoresponsive double hydrophilic diblock copolymer with tunable LCST
Publisher: American Chemical Society (ACS)
Date: 11-06-2015
Polymerization-Induced Self-Assembly: The Effect of End Group and Initiator Concentration on Morphology of Nanoparticles Prepared via RAFT Aqueous Emulsion Polymerization
Publisher: American Chemical Society (ACS)
Date: 31-08-2017
Cholesterol modified self-assemblies and their application to nanomedicine
Publisher: American Chemical Society (ACS)
Date: 22-06-2015
DOI: 10.1021/ACS.BIOMAC.5B00550
Abstract: Cholesterol is a ubiquitous molecule in biological systems, and in particular plays various important roles in mammalian cellular processes. The presence of cholesterol is integral to the structure and behavior of biological membranes, and profoundly influences membrane involvement in cellular mechanisms. This review focuses on the incorporation of cholesterol into synthetic nanomaterials and assemblies, focusing on LC phase behavior, morphology/self-organization and hydrophobic interactions, all important factors in the design of nanomedicines. We highlight cholesteryl conjugates, liposomes and polymeric micelles, focusing on self-assembly capabilities, drug encapsulation and intracellular delivery. An area of considerable interest identified in this review is the use of cholesteryl-functional vectors to deliver drugs or nucleic acids. Such applications depend on the ability of the nanoparticle carrier to associate with both the cellular and endosomal membrane.
Brushed Polyethylene Glycol and Phosphorylcholine as Promising Grafting Agents against Protein Binding
Publisher: Elsevier BV
Date: 02-2017
Local inflammation alters the lung disposition of a drug loaded pegylated liposome after pulmonary dosing to rats
Publisher: Elsevier BV
Date: 08-2019
DOI: 10.1016/J.JCONREL.2019.05.043
Abstract: The development of inhalable 'nanomedicines' based on biocompatible lipids and polymers is attracting increasing interest worldwide. Our understanding of how pulmonary inflammation impacts on lung distribution and clearance kinetics however, is limited. Similarly, there is limited information on how the inhaled delivery of biocompatible nanomaterials affects existing respiratory disease. We have addressed these knowledge gaps by describing and comparing the pulmonary pharmacokinetic behaviour of a
pH-Responsive Polymers for Improving the Signal-to-Noise Ratio of Hypoxia PET Imaging with [18F]Fluoromisonidazole
Publisher: Wiley
Date: 06-04-2020
Nano-assemblies of cationic mPEG brush block copolymers with gadolinium polyoxotungstate [Gd(W5O18)2]9− form stable, high relaxivity MRI contrast agents
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8NR01544A
Abstract: Nano-assemblies of gadolinium polyoxotungstate and cationic mPEG brush block copolymer demonstrated good stability and enhanced relaxivity at physiological pH.
Self-Assembly of Amphiphilic Polymeric Dendrimers Synthesized with Selective Degradable Linkages
Publisher: American Chemical Society (ACS)
Date: 12-12-2007
DOI: 10.1021/MA701993W
Cu(0)-mediated controlled/living radical polymerization: A tool for precise multiblock copolymer synthesis
Publisher: American Chemical Society
Date: 2014
Synthesis of functional core, star polymers via RAFT polymerization for drug delivery applications
Publisher: Wiley
Date: 12-04-2012
Abstract: Poly(oligoethylene glycol) methyl ether acrylate was polymerized via reversible addition fragmentation transfer polymerization (RAFT), and then chain extended in the presence of both a cross-linker and vinyl benzaldehyde (VBA), yielding monodisperse star polymers. The presence of aldehyde groups in the core was exploited to attach doxorubicin. The drug loading was controlled by the amount of VBA incorporated (until 28 wt% in drug). The doxorubicin release was studied at pH = 5.5 and 7.4 conditions representative of endosomal and extra cellular environments. In vitro studies revealed that the doxorubicin-conjugated star polymers had a level of cytotoxicity comparable to that found for free doxorubicin. Confocal microscopy and flow cytometry studies confirmed efficient cell uptake of the star polymers.
Original approach to multiblock copolymers via reversible addition–fragmentation chain transfer polymerization
Publisher: Wiley
Date: 19-04-2007
DOI: 10.1002/POLA.21985
Poly(2-isopropenyl-2-oxazoline)-a structural analogue to poly(vinyl azlactone) with Orthogonal Reactivity
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0PY00861C
Abstract: A modular copolymer platform based on two oxazole derivatives is presented. Post-polymerisation modifications revealed the potential to selectively modify the in idual side groups, providing access to functional copolymer libraries in the future.
Delivery of polymeric nanostars for molecular imaging and endoradiotherapy through the enhanced permeability and retention (EPR) effect
Publisher: Ivyspring International Publisher
Date: 2020
DOI: 10.7150/THNO.36777
Thiol-reactive star polymers functionalized with short ethoxy-containing moieties exhibit enhanced uptake in acute lymphoblastic leukemia cells
Publisher: Informa UK Limited
Date: 12-2019
DOI: 10.2147/IJN.S220326
Sequence-controlled methacrylic multiblock copolymers via sulfur-free RAFT emulsion polymerization
Publisher: Springer Science and Business Media LLC
Date: 17-10-2017
DOI: 10.1038/NCHEM.2634
Abstract: Translating the precise monomer sequence control achieved in nature over macromolecular structure (for ex le, DNA) to whole synthetic systems has been limited due to the lack of efficient synthetic methodologies. So far, chemists have only been able to synthesize monomer sequence-controlled macromolecules by means of complex, time-consuming and iterative chemical strategies such as solid-state Merrifield-type approaches or molecularly dissolved solution-phase systems. Here, we report a rapid and quantitative synthesis of sequence-controlled multiblock polymers in discrete stable nanoscale compartments via an emulsion polymerization approach in which a vinyl-terminated macromolecule is used as an efficient chain-transfer agent. This approach is environmentally friendly, fully translatable to industry and thus represents a significant advance in the development of complex macromolecule synthesis, where a high level of molecular precision or monomer sequence control confers potential for molecular targeting, recognition and biocatalysis, as well as molecular information storage.
Grafting of P(OEGA) Onto Magnetic Nanoparticles Using Cu(0) Mediated Polymerization: Comparing Grafting "from" and "to" Approaches in the Search for the Optimal Material, Design of Nanoparticle MRI Contrast Agents
Publisher: American Chemical Society (ACS)
Date: 02-08-2013
DOI: 10.1021/MA401250F
Biomimetic surface modification of honeycomb films via a grafting from approach
Publisher: American Chemical Society (ACS)
Date: 02-07-2010
DOI: 10.1021/LA1011567
Abstract: Hydrophobic isoporous membranes were fabricated using the "breath figure" method from polystyrene stars synthesized via ATRP. The living polymer chain ends at the surface of the films were then used, without further modification, in a "grafting-from" approach to grow surface-linked polyglycidyl methacrylate chains under conditions that maintained the regular honeycomb structure. This versatile functional surface was then used as a platform to build a small library of surfaces using a variety of simple chemistries: (i) the acid hydrolysis of the epoxide to form bis-alcohol groups and (ii) utilizing the "click-like" epoxide-amine reaction to functionalize the surface with a model biomolecule-(biotinamido)pentylamine. The successful modifications were confirmed by a combination of spectroscopic and biological means. Changes in the growth characteristics of nonmotile Psychrobacter sp. strain, SW5, on the honeycomb films, provided further evidence confirming changes in the hydrophobicity of the surface upon grafting.
Acid degradable and biocompatible polymeric nanoparticles for the potential codelivery of therapeutic agents
Publisher: American Chemical Society (ACS)
Date: 16-09-2011
DOI: 10.1021/MA201085Z
Raft mediated surface grafting of t-butyl acrylate onto an ethylene-propylene copolymer initiated by gamma radiation
Publisher: Wiley
Date: 07-02-2007
DOI: 10.1002/POLA.21868
Sulfoxide-Containing Polymer-Coated Nanoparticles Demonstrate Minimal Protein Fouling and Improved Blood Circulation
Publisher: Wiley
Date: 17-05-2020
Engineered hydrogen-bonded glycopolymer capsules and their interactions with antigen presenting cells
Publisher: American Chemical Society (ACS)
Date: 10-02-2017
Abstract: Hollow glycopolymer microcapsules were fabricated by hydrogen-bonded layer-by-layer (LbL) assembly, and their interactions with a set of antigen presenting cells (APCs), including dendritic cells (DCs), macrophages (MACs), and myeloid derived suppressor cells (MDSCs), were investigated. The glycopolymers were obtained by cascade postpolymerization modifications of poly(oligo(2-ethyl-2-oxazoline methacrylate)-stat-glycidyl methacrylate) involving the modification of the glycidyl groups with propargylamine and the subsequent attachment of mannose azide by copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). Multilayer assembly of the hydrogen-bonding pair (glycopolymer oly(methacrylic acid) (PMA)) onto planar and particulate supports (SiO
Molecular weight (hydrodynamic volume) dictates the systemic pharmacokinetics and tumour disposition of PolyPEG star polymers
Publisher: Elsevier BV
Date: 11-2015
DOI: 10.1016/J.NANO.2015.08.001
Abstract: Herein we report for the first time the biological fate of poly[(oligoethylene glycol) acrylate] (POEGA) star polymers synthesised via a versatile arm-first reversible addition-fragmentation chain transfer (RAFT) polymerisation approach. The biopharmaceutical behaviour of three different molecular weight (49, 64 and 94kDa) POEGA stars was evaluated in rats and nude mice bearing human MDA MB-231 tumours after intravenous administration. The 94kDa star polymer exhibited a longer plasma exposure time than the 49kDa or 64kDa star polymer an observation attributable to differences in the rates of both polymer biodegradation and urinary excretion. Tumour biodistribution also correlated with molecular weight and was greatest for the longest circulating 94kDa star. Different patterns of liver and spleen biodistribution were observed between mice and rats for the different sized polymers. The polymers were also well-tolerated in vivo and in vitro at therapeutic concentrations. Advances in nanotechnology has enabled scientists to produce nanoparticle as drug carriers in cancer therapeutics. In this article, the authors studied the biological fate of poly[(oligoethylene glycol) acrylate] (POEGA) star polymers of different size, after intravenous injections. This would allow the subsequent comparison to other drug delivery systems for better drug delivery.
Nitric Oxide (NO) Endows Arylamine-Containing Block Copolymers with Unique Photoresponsive and Switchable LCST Properties
Publisher: American Chemical Society (ACS)
Date: 15-03-2016
Polymers with acyl-protected perthiol chain termini as convenient building blocks for doubly responsive H2S-donating nanoparticles
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7PY01484H
Abstract: H 2 S-releasing polymers with an acyl-protected perthiol chain terminus were prepared using a simple, high yielding end-group modification process.
A hydrogel-based localized release of colistin for antimicrobial treatment of burn wound infection
Publisher: Wiley
Date: 13-09-2017
Abstract: There is an urgent unmet medical need for new treatments for wound and burn infections caused by multidrug-resistant Gram-negative "superbugs," especially the problematic Pseudomonas aeruginosa. In this work, the incorporation of colistin, a potent lipopeptide into a self-healable hydrogel (via dynamic imine bond formation) following the chemical reaction between the amine groups present in glycol chitosan and an aldehyde-modified poly(ethylene glycol), is reported. The storage module (G') of the colistin-loaded hydrogel ranges from 1.3 to 5.3 kPa by varying the amount of the cross-linker and colistin loading providing different options for topical wound healing. The majority of the colistin is released from the hydrogel within 24 h and remains active as demonstrated by both antibacterial in vitro disk diffusion and time-kill assays. Moreover and pleasingly, the colistin-loaded hydrogel performs almost equally well as native colistin against both the colistin-sensitive and also colistin-resistant P. aeruginosa strain in the in vivo animal "burn" infection model despite exhibiting a slower killing profile in vitro. Based on this antibiotic performance along with the biodegradability of the product, it is believed the colistin-loaded hydrogel to be a potential localized wound-healing formulation to treat burn wounds against microbial infection.
Formation of Tethered Polyacrylic Acid Loops in Core−Shell Micelles
Publisher: American Chemical Society (ACS)
Date: 19-05-2007
DOI: 10.1021/LA700724H
Abstract: Well-defined hiphilic four-arm star P(AA-b-STY) block copolymers have been dispersed in water to form core-shell micelles in which the shell consists of tethered PAA loops. The entropic penalty for having such loops resulted in a less densely packed PSTY core when compared to linear diblock copolymers of the same arm length. The surface of the shell is irregular because of the tethering points, but when cleaved the PAA chains extend to form a regular and relatively uniform corona.
Gadolinium-functionalized nanoparticles for application as magnetic resonance imaging contrast agents
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6PY01797E
Abstract: Polymerization-induced self-assembly (PISA) is an easily applied synthetic technique for the preparation of polymer nanoparticles with various shapes and at high concentrations.
Convergent Synthesis of Second Generation AB-Type Miktoarm Dendrimers Using “Click” Chemistry Catalyzed by Copper Wire
Publisher: American Chemical Society (ACS)
Date: 31-01-2008
DOI: 10.1021/MA702707E
Overcoming Surfactant-Induced Morphology Instability of Noncrosslinked Diblock Copolymer Nano-Objects Obtained by RAFT Emulsion Polymerization
Publisher: American Chemical Society (ACS)
Date: 10-01-2018
DOI: 10.1021/ACSMACROLETT.7B00978
Abstract: RAFT emulsion polymerization techniques including polymerization-induced self-assembly (PISA) and temperature-induced morphological transformation (TIMT) are widely used to produce noncrosslinked nano-objects with various morphologies. However, the worm, vesicle and lamellar morphologies produced by these techniques typically cannot tolerate the presence of added surfactants, thus limiting their potential applications. Herein we report the surfactant tolerance of noncrosslinked worms, vesicles, and lamellae prepared by RAFT emulsion polymerizations using poly(di(ethylene glycol) ethyl ether methacrylate-
Rapid Assessment of Nanoparticle Extravasation in a Microfluidic Tumor Model
Publisher: American Chemical Society (ACS)
Date: 29-03-2019
Cu(0)-Mediated Living Radical Polymerization: A Versatile Tool for Materials Synthesis
Publisher: American Chemical Society (ACS)
Date: 30-07-2016
Free volume and water uptake in a copolymer hydrogel series
Publisher: Wiley
Date: 02-1998
DOI: 10.1002/(SICI)1099-0488(199802)36:3<463::AID-POLB9>3.0.CO;2-S
Controlling Nanomaterial Size and Shape for Biomedical Applications via Polymerization-Induced Self-Assembly.
Publisher: Wiley
Date: 09-08-2018
Abstract: Rapid developments in the polymerization-induced self-assembly (PISA) technique have paved the way for the environmentally friendly production of nanoparticles with tunable size and shape for a erse range of applications. In this feature article, the biomedical applications of PISA nanoparticles and the substantial progress made in controlling their size and shape are highlighted. In addition to early investigations into drug delivery, applications such as medical imaging, tissue culture, and blood cryopreservation are also described. Various parameters for controlling the morphology of PISA nanoparticles are discussed, including the degree of polymerization of the macro-CTA and core-forming polymers, the concentration of macro-CTA and core-forming monomers, the solid content of the final products, the solution pH, the thermoresponsitivity of the macro-CTA, the macro-CTA end group, and the initiator concentration. Finally, several limitations and challenges for the PISA technique that have been recently addressed, along with those that will require further efforts into the future, will be highlighted.
Oxytocin in the Male Reproductive Tract; The Therapeutic Potential of Oxytocin-Agonists and-Antagonists
Publisher: Frontiers Media SA
Date: 22-10-2020
The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applications
Publisher: Springer Science and Business Media LLC
Date: 3
Synthesis of star polymers by RAFT polymerization as versatile nanoparticles for biomedical applications
Publisher: CSIRO Publishing
Date: 2017
DOI: 10.1071/CH17391
Abstract: The precise control of polymer chain architecture has been made possible by developments in polymer synthesis and conjugation chemistry. In particular, the synthesis of polymers in which at least three linear polymeric chains (or arms) are tethered to a central core has yielded a useful category of branched architecture, so-called star polymers. Fabrication of star polymers has traditionally been achieved using either a core-first technique or an arm-first approach. Recently, the ability to couple polymeric chain precursors onto a functionalized core via highly efficient coupling chemistry has provided a powerful new methodology for star synthesis. Star syntheses can be implemented using any of the living polymerization techniques using ionic or living radical intermediates. Consequently, there are innumerable routes to fabricate star polymers with varying chemical composition and arm numbers. In comparison with their linear counterparts, star polymers have unique characteristics such as low viscosity in solution, prolonged blood circulation, and high accumulation in tumour regions. These advantages mean that, far beyond their traditional application as rheology control agents, star polymers may also be useful in the medical and pharmaceutical sciences. In this account, we discuss recent advances made in our laboratory focused on star polymer research ranging from improvements in synthesis through to novel applications of the product materials. Specifically, we examine the core-first and arm-first preparation of stars using reversible addition–fragmentation chain transfer (RAFT) polymerization. Further, we also discuss several biomedical applications of the resulting star polymers, particularly those made by the arm-first protocol. Emphasis is given to applications in the emerging area of nanomedicine, in particular to the use of star polymers for controlled delivery of chemotherapeutic agents, protein inhibitors, signalling molecules, and siRNA. Finally, we examine possible future developments for the technology and suggest the further work required to enable clinical applications of these interesting materials.
Glutathione responsive polymers and their application in drug delivery systems
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C6PY01365A
Abstract: Materials which respond to biological cues are the subject of intense research interest due to their possible application in smart drug delivery vehicles.
The Pharmacokinetics and Biodistribution of a 64 kDa PolyPEG Star Polymer After Subcutaneous and Pulmonary Administration to Rats
Publisher: Elsevier BV
Date: 2016
DOI: 10.1016/J.XPHS.2015.11.038
Abstract: PolyPEG star polymers have potential utility as cost-effective polymeric drug delivery vehicles, and as such, it is important to develop an understanding of their biopharmaceutical behavior. Moreover, although a number of studies have evaluated the utility of PolyPEG stars in vitro, investigation of these novel materials in vivo has been limited. Herein, we evaluated the pharmacokinetics of a 64 kDa tritiated PEG-based star polymer after subcutaneous and pulmonary administration in rats. After subcutaneous administration, the star polymer showed near complete bioavailability (∼80%) and a similar organ biodistribution profile to the polymer after intravenous administration. After intratracheal instillation to the lungs, the star polymer showed limited bioavailability (∼3%), and most of the administered radiolabel was recovered in lung tissue and feces after 6 d. The data reported here suggest that star polymers display similar pharmaceutical behavior to PEGylated dendrimers after subcutaneous and inhaled delivery and may therefore be used as similar, but more cost-effective drug delivery vehicles.
Modification of graphene/graphene oxide with polymer brushes using controlled/living radical polymerization
Publisher: Wiley
Date: 08-05-2012
DOI: 10.1002/POLA.26094
Polymeric filomicelles and nanoworms: two decades of synthesis and application
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6PY00639F
Abstract: This review highlights the substantial progress in the syntheses and applications of filomicelles, an emerging nanomaterial with distinct and useful properties.
Application of Heterocyclic Polymers in the Ratiometric Spectrophotometric Determination of Fluoride
Publisher: American Chemical Society (ACS)
Date: 10-06-2015
DOI: 10.1021/MZ500782R
Linker chemistry dictates the delivery of a phototoxic organometallic rhenium(i) complex to human cervical cancer cells from core crosslinked star polymer nanoparticles
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8TB02464B
Abstract: Release of a photoactive Re( i ) complex from a polymer star via ketimine linkages gives improved phototoxicity.
Narrowing down chain length effects on the antibacterial action of guanylated oligomers
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D3PY00203A
Abstract: Synthesis of discrete guanylated antimicrobial oligomers through reversible addition fragmentation chain transfer (RAFT) polymerization followed by flash chromatography is described.
Synthesis of Complex Macromolecules Using Iterative Copper(0)-Mediated Radical Polymerization
Publisher: Wiley
Date: 12-05-2014
DOI: 10.1002/POLA.27220
Reproducible Access to Tunable Morphologies via the Self-Assembly of an Amphiphilic Diblock Copolymer in Water
Publisher: American Chemical Society (ACS)
Date: 19-03-2015
Thiol-responsive lyotropic liquid crystals exhibit triggered phase re-arrangement and hydrogen sulfide (H2S) release
Publisher: Elsevier BV
Date: 05-2022
DOI: 10.1016/J.JCIS.2021.12.180
Abstract: Hydrogen sulfide (H
Polymeric micelles with anti-virulence activity against Candida albicans in a single- and dual-species biofilm
Publisher: Springer Science and Business Media LLC
Date: 13-03-2021
Related Organisations
Related Funding Activities
Linkage Projects - Grant ID: LP140100284
Start Date: 10-2014
End Date: 06-2017
Amount: $450,000.00
Funder: Australian Research Council
View Funded ActivityDiscovery Projects - Grant ID: DP0878733
Start Date: 2008
End Date: 12-2010
Amount: $475,000.00
Funder: Australian Research Council
View Funded ActivityDiscovery Projects - Grant ID: DP200102829
Start Date: 01-2020
End Date: 12-2023
Amount: $430,000.00
Funder: Australian Research Council
View Funded ActivityLinkage Projects - Grant ID: LP0990189
Start Date: 01-2010
End Date: 12-2014
Amount: $390,000.00
Funder: Australian Research Council
View Funded ActivityLinkage Infrastructure, Equipment And Facilities - Grant ID: LE0989946
Start Date: 04-2009
End Date: 12-2010
Amount: $357,000.00
Funder: Australian Research Council
View Funded ActivityDiscovery Projects - Grant ID: DP170102950
Start Date: 2017
End Date: 12-2019
Amount: $322,500.00
Funder: Australian Research Council
View Funded ActivityDiscovery Projects - Grant ID: DP220103654
Start Date: 04-2022
End Date: 03-2025
Amount: $436,908.00
Funder: Australian Research Council
View Funded Activity