ORCID Profile
0000-0001-5593-348X
Current Organisation
Universitat de Barcelona
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Springer Science and Business Media LLC
Date: 29-09-2016
Publisher: American Association for the Advancement of Science (AAAS)
Date: 04-05-2018
Abstract: Environmental DNA reveals unsuspected shark ersity and calls for monitoring and protection of residual populations.
Publisher: Elsevier BV
Date: 12-2021
Publisher: Frontiers Media SA
Date: 02-02-2022
DOI: 10.3389/FMARS.2022.809659
Abstract: Traditional morphological methods for species identification are highly time consuming, especially for small organisms, such as Foraminifera, a group of shell-building microbial eukaryotes. To analyze large amounts of s les more efficiently, species identification methods have extended to molecular tools in the last few decades. Although a wide range of phyla have good markers available, for Foraminifera only one hypervariable marker from the ribosomal region (18S) is widely used. Recently a new mitochondrial marker cytochrome oxidase subunit 1 (COI) has been sequenced. Here we investigate whether this marker has a higher potential for species identification compared to the ribosomal marker. We explore the genetic variability of both the 18S and COI markers in 22 benthic foraminiferal morphospecies (orders Miliolida and Rotaliida). Using single-cell DNA, the genetic variability within specimens (intra) and between specimens (inter) of each species was assessed using next-generation sequencing. Amplification success rate was twice as high for COI (151/200 specimens) than for 18S (73/200 specimens). The COI marker showed greatly decreased intra- and inter-specimen variability compared to 18S in six out of seven selected species. The 18S phylogenetic reconstruction fails to adequately cluster multiple species together in contrast to COI. Additionally, the COI marker helped recognize misclassified specimens difficult to morphologically identify to the species level. Integrative taxonomy, combining morphological and molecular characteristics, provides a robust picture of the foraminiferal species ersity. Finally, we suggest the use of a set of sequences (two or more) to describe species showing intra-genomic variability additionally to using multiple markers. Our findings highlight the potential of the newly discovered mitochondrial marker for molecular species identification and metabarcoding purposes.
Publisher: Springer Science and Business Media LLC
Date: 14-06-2018
Publisher: Wiley
Date: 21-08-2020
DOI: 10.1111/MEC.15564
Publisher: Public Library of Science (PLoS)
Date: 17-09-2012
Location: Norway
Location: United Kingdom of Great Britain and Northern Ireland
Location: No location found
No related grants have been discovered for Owen S. Wangensteen.