ORCID Profile
0000-0002-8386-0131
Current Organisation
Southern Cross University
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Medicinal and Biomolecular Chemistry | Biologically Active Molecules | Biochemistry And Cell Biology Not Elsewhere Classified | Characterisation of Biological Macromolecules | Biochemistry and Cell Biology | Microbiology Not Elsewhere Classified | Ecology And Evolution Not Elsewhere Classified | Nanochemistry and Supramolecular Chemistry | Chemical Characterisation of Materials | Proteins and Peptides |
Expanding Knowledge in the Chemical Sciences | Expanding Knowledge in the Biological Sciences | Expanding Knowledge in the Agricultural and Veterinary Sciences | Biological sciences | Other
Publisher: Elsevier BV
Date: 04-2022
DOI: 10.1016/J.MARENVRES.2022.105590
Abstract: Climate change is impacting living marine resources, whilst concomitantly, global reliance on seafood as a source of nutrition is increasing. Here we review an emerging research frontier, identifying significant impacts of climate-driven environmental change on the nutritional and sensory quality of seafood, and implications for human health. We highlight that changing ocean temperature, pH and salinity can lead to reductions in seafood macro and micronutrients, including essential nutrients such as protein and lipids. However, the nutritional quality of seafood appears to be more resilient in taxa that inhabit naturally variable environments such as estuaries and shallow near-coastal habitats. We develop criteria for assessing confidence in categorising the nutritional quality of seafood as vulnerable or resilient to climate change. The application of this criteria to a subset of seafood nutritional studies demonstrates confidence levels are generally low and could be improved by more realistic experimental designs and research collaboration. We highlight knowledge gaps to guide future research in this emerging field.
Publisher: Elsevier BV
Date: 2007
DOI: 10.1016/J.BMC.2006.10.035
Abstract: A range of substituted 1H-indole-2,3-diones (isatins) were synthesized using standard procedures and their cytotoxicity evaluated against the human monocyte-like histiocytic lymphoma (U937) cell line in vitro. SAR studies identified C(5), C(6), and C(7) substitution greatly enhanced activity with some di- and tri-halogenated isatins giving IC(50) values <10 microM. Of the 23 compounds tested, four were selected for further screening against a panel of five human cancer cell lines. These compounds, in general, showed greater selectivity toward leukemia and lymphoma cells over breast, prostate, and colorectal carcinoma cell lines. The most active compound, 5,6,7-tribromoisatin (2p), was found to be antiproliferative at low micromolar concentrations and also activated the effector caspases 3 and 7 in a dose-dependent manner. These results indicate that di- and tri-substituted isatins may be useful leads for anticancer drug development in the future.
Publisher: Elsevier BV
Date: 06-2023
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 2023
Publisher: MDPI AG
Date: 16-03-2015
DOI: 10.3390/MD13031410
Publisher: Wiley
Date: 27-04-2010
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 2023
Publisher: Elsevier BV
Date: 2014
DOI: 10.1016/J.FSI.2013.10.026
Abstract: Disease is caused by a complex interaction between the pathogen, environment, and the physiological status of the host. Determining how host ontogeny interacts with water temperature to influence the antiviral response of the Pacific oysters, Crassostrea gigas, is a major goal in understanding why juvenile Pacific oysters are dying during summer as a result of the global emergence of a new genotype of the Ostreid herpesvirus, termed OsHV-1 μvar. We measured the effect of temperature (12 vs 22 °C) on the antiviral response of adult and juvenile C. gigas injected with poly I:C. Poly I:C up-regulated the expression of numerous immune genes, including TLR, MyD88, IκB-1, Rel, IRF, MDA5, STING, SOC, PKR, Viperin and Mpeg1. At 22 °C, these immune genes showed significant up-regulation in juvenile and adult oysters, but the majority of these genes were up-regulated 12 h post-injection for juveniles compared to 26 h for adults. At 12 °C, the response of these genes was completely inhibited in juveniles and delayed in adults. Temperature and age had no effect on hemolymph antiviral activity against herpes simplex virus (HSV-1). These results suggest that oysters rely on a cellular response to minimise viral replication, involving recognition of virus-associated molecular patterns to induce host cells into an antiviral state, as opposed to producing broad-spectrum antiviral compounds. This cellular response, measured by antiviral gene expression of circulating hemocytes, was influenced by temperature and oyster age. We speculate whether the vigorous antiviral response of juveniles at 22 °C results in an immune-mediated disorder causing mortality.
Publisher: Elsevier BV
Date: 12-2023
Publisher: Wiley
Date: 17-05-2017
DOI: 10.1002/RCM.7869
Abstract: Nanostructure-based mass spectrometry imaging (MSI) is a promising technology for molecular imaging of small molecules, without the complex chemical background typically encountered in matrix-assisted molecular imaging approaches. Here, we have enhanced these surfaces with silver (Ag) to provide a second tier of MSI data from a single s le. MSI data was acquired through the application of laser desorption/ionization mass spectrometry to biological s les imprinted onto desorption/ionization on silicon (DIOS) substrates. Following initial analysis, ultra-thin Ag layers were overlaid onto the followed by MSI analysis (Ag-DIOS MSI). This approach was first demonstrated for fingermark small molecules including environmental contaminants and sebum components. Subsequently, this bimodal method was translated to lipids and metabolites in fore-stomach sections from a 6-bromoisatin chemopreventative murine mouse model. DIOS MSI allowed mapping of common ions in fingermarks as well as 6-bromoisatin metabolites and lipids in murine fore-stomach. Furthermore, DIOS MSI was complemented by the Ag-DIOS MSI of Ag-adductable lipids such as wax esters in fingermarks and cholesterol in murine fore-stomach. Gastrointestinal acid condensation products of 6-bromoisatin, such as the 6,6'-dibromoindirubin mapped herein, are very challenging to isolate and characterize. By re-analyzing the same tissue imprints, this metabolite was readily detected by DIOS, placed in a tissue-specific spatial context, and subsequently overlaid with additional lipid distributions acquired using Ag-DIOS MSI. The ability to place metabolite and lipid classes in a tissue-specific context makes this novel method suited to MSI analyses where the collection of additional information from the same s le maximises resource use, and also maximises the number of annotated small molecules, in particular for metabolites that are typically undetectable with traditional platforms. Copyright © 2017 John Wiley & Sons, Ltd.
Publisher: Wiley
Date: 09-11-2022
DOI: 10.1002/AQC.3895
Abstract: The temperate soft coral Dendronephthya australis has been listed as Endangered in NSW, Australia, owing to its restricted geographic range, rapidly declining populations, and a number of escalating threats. Research to assess practical methods for aquarium cultivation of D. australis for rehabilitation of wild populations is considered a high priority. Previous attempts at restoration by transplanting D. australis fragments have had limited success, but identified key challenges, which included achieving attachment to substrates and identifying appropriate foods to maximize survival. This study presents the results of experiments promoting the attachment of cuttings (nubbins) removed from donor colonies to moveable substrates (small concrete discs). Using a balanced orthogonal experimental design, three feeding regimes were tested: live artemia, live rotifers and dry powdered zooplankton. Donor colony survival and growth were also monitored over a 6 month period in the field. An overall nubbin survival rate of 100% was achieved under all three feeding regimes. Attachment rates were similar across feeding treatments, although nubbin behavioural responses (percentage of open polyps before feeding) suggested some preference for live rotifers. The cutting removal process had no significant effect on donor colonies in the wild, indicating that this is a sustainable approach for restoration. Overall, this study substantially advances the methodology for maintaining an Endangered azooxanthellate soft coral in aquaria and provides valuable input for management programmes aimed at implementing transplantation into the wild to aid species recovery.
Publisher: Elsevier BV
Date: 08-2014
DOI: 10.1016/J.FSI.2014.06.010
Abstract: Oyster farming is one of the most important aquaculture industries in the world. However, its productivity is increasingly limited by viral disease and we do not yet have management practices, such as protective vaccination, that can control these disease outbreaks. Hence, in the current study we investigated the expression of known anti-viral genes in oysters (Crassostrea gigas) in response to primary and secondary encounter with a virus associated molecular pattern (dsRNA), and tested whether a common form of epigenetic gene regulation (DNA methylation) was associated with the expression of these anti-viral genes. Injection of dsRNA into the adductor muscle resulted in the rapid and transient expression of virus recognition receptors (TLR & MDA5), whereas several anti-viral signalling (IRF & SOC-1) and effector (PKR & viperin) genes were still up-regulated at one week post primary challenge (p 0.05). These results collectively suggest that C. gigas does not have an enhanced anti-viral gene response (immune-priming) to secondary dsRNA challenge and that the sustained up-regulation of anti-viral signalling and effector genes following primary challenge is unlikely to be associated with upstream DNA methylation levels.
Publisher: Elsevier BV
Date: 02-2017
DOI: 10.1016/J.JTHERBIO.2017.01.008
Abstract: Predicted global climate change has prompted numerous studies of thermal tolerances of marine species. The upper thermal tolerance is unknown for most marine species, but will determine their vulnerability to ocean warming. Gastropods in the family Turbinidae are widely harvested for human consumption. To investigate the responses of turbinid snails to future conditions we determined critical thermal maxima (CTMax) and preferred temperatures of Turbo militaris and Lunella undulata from the tropical-temperate overlap region of northern New South Wales, on the Australian east coast. CTMax were determined at two warming rates: 1°C/30min and 1°C/12h. The number of snails that lost attachment to the tank wall was recorded at each temperature increment. At the faster rate, T. militaris had a significantly higher CTMax (34.0°C) than L. undulata (32.2°C). At the slower rate the mean of both species was lower and there was no significant difference between them (29.4°C for T. militaris and 29.6°C for L. undulata). This is consistent with differences in thermal inertia possibly allowing animals to tolerate short periods at higher temperatures than is possible during longer exposure times, but other mechanisms are not discounted. The thermoregulatory behaviour of the turban snails was determined in a horizontal thermal gradient. Both species actively sought out particular temperatures along the gradient, suggesting that behavioural responses may be important in ameliorating short-term temperature changes. The preferred temperatures of both species were higher at night (24.0°C and 26.0°C) than during the day (22.0°C and 23.9°C). As the snails approached their preferred temperature, net hourly displacement decreased. Preferred temperatures were within the average seasonal seawater temperature range in this region. However, with future predicted water temperature trends, the species could experience increased periods of thermal stress, possibly exceeding CTMax and potentially leading to range contractions.
Publisher: Elsevier BV
Date: 10-2014
DOI: 10.1016/J.ANTIVIRAL.2014.08.010
Abstract: Viruses belonging to the family Malacoherpesviridae currently pose a serious threat to global production of the Pacific oyster, Crassostrea gigas. Hemolymph extracts from C. gigas are known to have potent antiviral activity. The compound(s) responsible for this broad-spectrum antiviral activity in oyster hemolymph have not been identified. The objective of this study was to identify these antiviral compound(s) and establish whether hemolymph antiviral activity is under genetic control in the Australian C. gigas population. Hemolymph antiviral activity of 18 family lines of C. gigas were assayed using a herpes simplex virus type 1 (HSV-1) and Vero cell plaque reduction assay. Differences in anti-HSV-1 activity between the family lines were observed (p<0.001) with heritability estimated to be low (h(2)=0.21). A glycoprotein that inhibits HSV-1 replication was identified by resolving oyster hemolymph by native-polyacrylamide gel electrophoresis (PAGE) and assaying extracted protein fractions using the HSV-1 and Vero cell plaque assay. Highest anti-HSV-1 activity corresponded with an N-linked glycoprotein with an estimated molecular mass of 21kDa under non-reducing SDS-PAGE conditions. Amino acid sequencing by tandem mass spectrometry revealed this protein matched the major hemolymph protein, termed cavortin. Our results provide further evidence that cavortin is a multifunctional protein involved in immunity and that assays associated with its activity might be useful for marker-assisted selection of disease resistant oysters.
Publisher: Wiley
Date: 04-2006
Publisher: Informa UK Limited
Date: 19-09-2018
Publisher: Springer Science and Business Media LLC
Date: 09-2015
DOI: 10.1038/SREP13408
Abstract: Despite significant advances in chemical ecology, the biodistribution, temporal changes and ecological function of most marine secondary metabolites remain unknown. One such ex le is the association between choline esters and Tyrian purple precursors in muricid molluscs. Mass spectrometry imaging (MSI) on nano-structured surfaces has emerged as a sophisticated platform for spatial analysis of low molecular mass metabolites in heterogeneous tissues, ideal for low abundant secondary metabolites. Here we applied desorption-ionisation on porous silicon (DIOS) to examine in situ changes in biodistribution over the reproductive cycle. DIOS-MSI showed muscle-relaxing choline ester murexine to co-localise with tyrindoxyl sulfate in the biosynthetic hypobranchial glands. But during egg-laying, murexine was transferred to the capsule gland and then to the egg capsules, where chemical ripening resulted in Tyrian purple formation. Murexine was found to tranquilise the larvae and may relax the reproductive tract. This study shows that DIOS-MSI is a powerful tool that can provide new insights into marine chemo-ecology.
Publisher: Wiley
Date: 15-08-2008
Publisher: Elsevier BV
Date: 06-2007
Publisher: Wiley
Date: 05-04-2016
DOI: 10.1002/FSN3.360
Publisher: Elsevier BV
Date: 10-2018
DOI: 10.1016/J.MARENVRES.2018.08.009
Abstract: Rising levels of atmospheric carbon dioxide are driving ocean warming and acidification. This could cause stress resulting in decreases in nutritional quality of marine species for human consumption, if environmental changes go beyond the optimal range for harvested species. To evaluate this, we used ambient and near-future elevated temperatures and pCO
Publisher: American Chemical Society (ACS)
Date: 08-10-2012
DOI: 10.1021/AC3027433
Abstract: Desorption/ionization on porous silicon-mass spectrometry (DIOS-MS) is an attractive alternative to conventional matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the analysis of low molecular weight compounds. Porous silicon (pSi) chips are also suitable as support for mass spectrometry imaging (MSI). Here, we report an implementation of DIOS-MSI using the biosynthetic organs of a marine mollusc for proof of principle. The tissue section is st ed onto a fluorocarbon-functionalized pSi chip, which extracts and traps small hydrophobic molecules from the tissue under retention of their relative spatial distribution. The section is subsequently removed and the chip is imaged without any remaining tissue. We apply this novel tissue contact printing approach to investigate the distribution of biologically active brominated precursors to Tyrian purple in the hypobranchial gland of the marine mollusc, Dicathais orbita, using DIOS-MSI. The tissue contact printing is also compatible with other types of desorption/ionization surfaces, such as nanoassisted laser desorption/ionization (NALDI) targets.
Publisher: Public Library of Science (PLoS)
Date: 26-10-2017
Publisher: Springer Science and Business Media LLC
Date: 26-08-2019
DOI: 10.1038/S41598-019-48533-2
Abstract: Indole derivatives are a structurally erse group of compounds found in food, toxins, medicines, and produced by commensal microbiota. On contact with acidic stomach conditions, indoles undergo condensation to generate metabolites that vary in solubility, activity and toxicity as they move through the gut. Here, using halogenated ions, we map promising chemo-preventative indoles, i) 6-bromoisatin (6Br), ii) the mixed indole natural extract (NE) 6Br is found in, and iii) the highly insoluble metabolites formed in vivo using desorption/ionisation on porous silicon-mass spectrometry imaging (DIOS-MSI). The functionalised porous silicon architecture allowed insoluble metabolites to be detected that would otherwise evade most analytical platforms, providing direct evidence for identifying the therapeutic component, 6Br, from the mixed indole NE. As a therapeutic lead, 0.025 mg/g 6Br acts as a chemo-preventative compound in a 12 week genotoxic mouse model at this dose 6Br significantly reduces epithelial cell proliferation, tumour precursors (aberrant crypt foci ACF) and tumour numbers while having minimal effects on liver, blood biochemistry and weight parameters compared to controls. The same could not be said for the NE where 6Br originates, which significantly increased liver damage markers. DIOS-MSI revealed a large range of previously unknown insoluble metabolites that could contribute to reduced efficacy and increased toxicity.
Start Date: 2001
End Date: 12-2002
Amount: $269,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 2014
End Date: 12-2015
Amount: $2,000,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 05-2017
End Date: 12-2017
Amount: $450,000.00
Funder: Australian Research Council
View Funded Activity