ORCID Profile
0000-0003-2590-5394
Current Organisation
Universiti Tenaga Nasional
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Publisher: Public Library of Science (PLoS)
Date: 07-10-2016
Publisher: American Chemical Society (ACS)
Date: 08-05-2017
Abstract: Cattle, buffalo, and porcine materials are widely adulterated, and their quantification might safeguard health, religious, economic, and social sanctity. Recently, conventional polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) assays have been documented but they are just suitable for identification, cannot quantify adulterations. We described here a quantitative tetraplex real-time PCR assay with TaqMan Probes to quantify contributions from cattle, buffalo, and porcine materials simultaneously. Amplicon-sizes were very short (106-, 90-, and 146-bp for cattle, buffalo, and porcine) because longer targets could be broken down, bringing serious ambiguity in molecular diagnostics. False negative detection was eliminated through an endogenous control (141-bp site of eukaryotic 18S rRNA). Analysis of 27 frankfurters and 27 meatballs reflected 84-115% target recovery at 0.1-10% adulterations. Finally, a test of 36 commercial products revealed 71% beef frankfurters, 100% meatballs, and 85% burgers contained buffalo adulteration, but no porcine was found in beef products.
Publisher: Informa UK Limited
Date: 04-02-2021
Publisher: Springer Science and Business Media LLC
Date: 09-01-2021
Publisher: Informa UK Limited
Date: 05-07-2017
DOI: 10.1080/10408398.2016.1264361
Abstract: Gelatin is a highly purified animal protein of pig, cow, and fish origins and is extensively used in food, pharmaceuticals, and personal care products. However, the acceptability of gelatin products greatly depends on the animal sources of the gelatin. Porcine and bovine gelatins have attractive features but limited acceptance because of religious prohibitions and potential zoonotic threats, whereas fish gelatin is welcomed in all religions and cultures. Thus, source authentication is a must for gelatin products but it is greatly challenging due to the breakdown of both protein and DNA biomarkers in processed gelatins. Therefore, several methods have been proposed for gelatin identification, but a comprehensive and systematic document that includes all of the techniques does not exist. This up-to-date review addresses this research gap and presents, in an accessible format, the major gelatin source authentication techniques, which are primarily nucleic acid and protein based. Instead of presenting these methods in paragraph form which needs much attention in reading, the major methods are schematically depicted, and their comparative features are tabulated. Future technologies are forecasted, and challenges are outlined. Overall, this review paper has the merit to serve as a reference guide for the production and application of gelatin in academia and industry and will act as a platform for the development of improved methods for gelatin authentication.
Publisher: Wiley
Date: 03-08-2021
DOI: 10.1111/AOR.14045
Abstract: The primary role of bone tissue engineering is to reconcile the damaged bones and facilitate the speedy recovery of the injured bones. However, some of the investigated metallic implants suffer from stress‐shielding, palpability, biocompatibility, etc . Consequently, the biodegradable scaffolds fabricated from polymers have gathered much attention from researchers and thus helped the tissue engineering sector by providing many alternative materials whose functionality is similar to that of natural bones. Herein, we present the fabrication and testing of a novel composite, magnesium (Mg)‐doped hydroxyapatite (HAp) glazed onto polylactic acid (PLA) scaffolds where polyvinyl alcohol (PVA) used as a binder. For the composite formation, Creality Ender‐3 pro High Precision 3D Printer with Shape tool 3D Technology on an FSD machine operated by Catia design software was employed. The composite has been characterized for the crystallinity (XRD), surface functionality (FTIR), morphology (FESEM), biocompatibility (hemolytic and protein absorption), and mechanical properties (stress‐strain and maximum compressive strength). The powder XRD analysis confirmed the semicrystalline nature and intact structure of HAp even after doping with Mg, while FTIR studies for the successful formation of Mg‐HAp/PVA@PLA composite. The FESEM provided analysis indicated for the 3D porous architecture and well‐defined morphology to efficiently transport the nutrients, and the biocompatibility studies are supporting that the composite for blood compatible with the surface being suitable enough for the protein absorption. Finally, the composite's antibacterial activity (against Staphylococcus aureus and Escherichia coli ) and the test of mechanical properties supported for the enhanced inhibition of active growth of microorganisms and maximum compressive strength, respectively. Based on the research outcomes of biocompatibility, antibacterial activity, and mechanical resistance, the fabricated Mg‐HAp/PVA@PLA composite suits well as a promising biomaterial platform for orthopedic applications by functioning towards the open reduction internal fixation of bone fractures and internal repairs.
Publisher: IOP Publishing
Date: 02-2021
Abstract: Conducting polymers are gaining importance in recent years for their remarkable electrical and ionic properties and so, in order to further improve the fundamental properties, they are being integrated with iron oxide (Fe 3 O 4 ) nanoparticles (NPs). Therefore, in the present study, polypyrrole (PPy) doped with iron oxide NPs to form PPy@Fe 3 O 4 nanocomposite by adopting a simple chemical precipitation method of in situ polymerization. The various spectroscopic (UV–vis, FTIR, Raman, XRD, and XPS), and electron microscopic (FESEM and HRTEM) analysis were employed to study the effects of Fe 3 O 4 NPs onto the polymer mixture. The analysis of results revealed the successful doping of Fe 3 O 4 NPs onto the PPy polymer and also, the Raman spectral studies confirmed not only for the stabilization of doped nanocomposites in its polaronic form but also prevented from the auxiliary oxidation of polymer.
No related grants have been discovered for M. A. Motalib Hossain.