ORCID Profile
0000-0003-3866-5836
Current Organisations
Lee Kuan Yew School of Public Policy
,
University of California Los Angeles
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Publisher: Elsevier BV
Date: 11-1995
Abstract: We present evidence that the HIV-1 Tat protein and the RNA-dependent cellular protein kinase, PKR, interact with each other both in vitro and in vivo. Using GST fusion chromatography, we demonstrate that PKR, interacts directly with the HIV-1 Tat protein. The region in Tat sufficient for binding PKR maps within amino acids 20 to 72. In in vitro assays, the two-exon form of Tat (Tat 86) was phosphorylated by PKR, while the one exon form of Tat (Tat 72) inhibited PKR autophosphorylation and substrate phosphorylation. The ability of Tat to interact with PKR was demonstrated in both yeast and mammalian cells. Expression of PKR in yeast results in a growth suppressor phenotype which was reversed by coexpression of a one exon form of Tat. Expression of Tat 72 in HeLa cells resulted in direct interaction with PKR as detected by coimmunprecipitation with a Tat antibody. Tat and PKR also form a coimmunoprecipitable complex in cell-free extracts prepared from productively infected T lymphocytes. The interaction of Tat with PKR provides a potential mechanism by which HIV could suppress the interferon system.
Publisher: MDPI AG
Date: 07-06-2021
DOI: 10.3390/SU13116502
Abstract: While policy study of smart city developments is gaining traction, it falls short of understanding and explaining knowledge transfers across national borders and cities. This article investigates how transboundary learning occurs through the initiation and development of a regional smart cities network: the ASEAN Smart Cities Network (ASCN). The article conducts an in-depth case study from data collected through key informant interviews and document analysis. Spearheaded by Singapore in 2017, ASCN is seen as a soft power extension for Singapore, a branding tool for ASEAN, and a symbiotic platform between the private sector and governments in the region. Most transboundary knowledge transfers within the ASCN are voluntary transfers of policy ideas. Effective branding, demand for knowledge, availability of alternative funding options, enthusiasm from the private actors, and heightened interest from other major economies are highlighted as facilitators of knowledge transfer. However, the complexity of governance structures, lack of political will and resources, limited policy capacity, and lack of explicit operational and regulatory mechanisms hinder transboundary learning. The article concludes that transboundary learning should go beyond exchanges of ideas and recommends promoting facilitators of knowledge transfer, building local policy capacity, encouraging collaborative policy transfer, and transiting from an information-sharing platform to tool/instrument-based transfer.
Location: United States of America
Location: India
Location: United States of America
Location: United States of America
No related grants have been discovered for Kritika Sha.