ORCID Profile
0000-0003-0912-6913
Current Organisation
Badan Riset dan Inovasi Nasional Republik Indonesia
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Publisher: Wiley
Date: 18-10-2011
DOI: 10.1111/J.1365-2958.2011.07855.X
Abstract: Resistance to quinoline antimalarial drugs has emerged in different parts of the world and involves sets of discrete mutational changes in pfcrt and pfmdr1 in the human malaria parasite Plasmodium falciparum. To better understand how the different polymorphic haplotypes of pfmdr1 and pfcrt contribute to drug resistance, we have conducted a linkage analysis in the F1 progeny of a genetic cross where we assess both the susceptibility and the amount of accumulation of chloroquine, amodiaquine, quinine and quinidine. Our data show that the different pfcrt and pfmdr1 haplotypes confer drug-specific responses which, depending on the drug, may affect drug accumulation or susceptibility or both. These findings suggest that PfCRT and PfMDR1 are carriers of antimalarial drugs, but that the interaction with a drug interferes with the carriers' natural transport function such that they are now themselves targets of these drugs. How well a mutant PfCRT and PfMDR1 type copes with its competing transport functions is determined by its specific sets of amino acid substitutions.
Publisher: Rasayan Journal of Chemistry
Date: 2023
DOI: 10.31788/RJC.2023.1618092
Abstract: Mycobacterium tuberculosis drug-resistant strains have emerged, creating a new difficulty in the treatment of tuberculosis around the world and prompting the World Health Organization to declare TB an international emergency. With TB treatment resistance increasing, developing more potent vaccinations can ensure the TB epidemic is stopped or reduced. The MPT64 gene was lified from the genomic DNA of the M. tuberculosis local strain using polymerase chain reaction (PCR) in vitro and then transferred into the pGEM-T Easy-Mpt83+Mpt64 vector and expressed in the E. coli BL21 (DE3) strain. Plasmid DNA was isolated and lified using PCR, followed by DNA sequencing. Subcloned into the expression vector pTrcHisA on NheI/HindIII cloning site before being converted into the E. coli BL21 (DE3) strain were the correct recombinant MPT83 and MPT64 gene fusions. The white recombinant colony was propagated and maintained using 40 µM IPTG, then cells and protein recombinants were harvested, and SDS-PAGE electrophoresis was used to identify it. The protein was then purified with a 6XHis tagged-agarose beads purification kit. The results showed that the MPT83 and MPT64 gene fusion was successfully produced, expressed, and purified. The fusion protein of MPT83 and MPT64 with a His tag from the expression vector had a molecular weight of about 46 kDa and was expressed as a soluble protein. Cloned from the host strain of the E. coli BL21 (DE3) strain cell, the fusion genes were successfully produced in the bacteria. Pathways to tuberculosis diagnoses are laid by the purified recombinant fusion proteins, MPT83 and MPT64, and may be more successful in the future as a vaccine candidate.
Publisher: Atlantis Press International BV
Date: 2023
Publisher: F1000 Research Ltd
Date: 03-01-2023
DOI: 10.12688/F1000RESEARCH.123181.1
Abstract: Background: The enhanced green fluorescent protein (EGFP) gene is a reporter gene that can be used to optimize protein isolation procedures and the functional working of a transduction protein. EGFP, with the addition of eleven arginine residues, has been engineered to functionally improve the protein transduction process, which can later be used for cell reprogramming like induced pluripotent stem cells. The addition of six histidine amino acid residues at its C-terminal is intended for the protein isolation process using the His-tag antibody. Methods: The study aimed to investigate the optimization of the EGFP polyarginine protein expression in Saccharomyces cerevisiae in sufficient quantities for the protein isolation stage. This study also analyzed EGFP expression without polyarginine to analyze the polyarginine addition effect on expression processes. Protein expression was qualitatively measured by looking at expression fluorescence and protein levels of EGFP and EGFP - PolyR proteins. Results: Bands on Western Blots with 6×His-tag monoclonal antibody (primary antibody) and Goat anti-mouse IgG HRP (secondary antibody) showed the EGFP polyarginine and EGFP proteins were expressed in Saccharomyces cerevisiae INVSc1 at relatively low levels. The lyticase incubation time modification and administration of 3-5 kDa microfilter to concentrate increased the yield of isolated protein. Conclusions: The sufficient amount of protein isolation in S. cerevisiae can be achieved by using lyticase and sonicators combination for the lysis process.
Publisher: Public Library of Science (PLoS)
Date: 15-05-2014
Publisher: CSIR-National Institute of Science Communication and Policy Research (NIScPR)
Date: 2022
Location: United Kingdom of Great Britain and Northern Ireland
Location: Indonesia
No related grants have been discovered for Astutiati Nurhasanah.