ORCID Profile
0000-0001-5056-9893
Current Organisations
University of Manchester
,
Universitat de Barcelona - Campus Mundet
,
Institute of Molecular and Cellular Biology of Plants
,
Corporacion Colombiana de Investigación Agropecuaria
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Publications
Tomato geranylgeranyl diphosphate synthase isoform 1 is involved in the stress‐triggered production of diterpenes in leaves and strigolactones in roots
Publisher: Wiley
Date: 28-06-2023
DOI: 10.1111/NPH.19109
Abstract: Carotenoids are photoprotectant pigments and precursors of hormones such as strigolactones (SL). Carotenoids are produced in plastids from geranylgeranyl diphosphate (GGPP), which is erted to the carotenoid pathway by phytoene synthase (PSY). In tomato ( Solanum lycopersicum ), three genes encode plastid‐targeted GGPP synthases ( SlG1 to SlG3 ) and three genes encode PSY isoforms ( PSY1 to PSY3 ). Here, we investigated the function of SlG1 by generating loss‐of‐function lines and combining their metabolic and physiological phenotyping with gene co‐expression and co‐immunoprecipitation analyses. Leaves and fruits of slg1 lines showed a wild‐type phenotype in terms of carotenoid accumulation, photosynthesis, and development under normal growth conditions. In response to bacterial infection, however, slg1 leaves produced lower levels of defensive GGPP‐derived diterpenoids. In roots, SlG1 was co‐expressed with PSY3 and other genes involved in SL production, and slg1 lines grown under phosphate starvation exuded less SLs. However, slg1 plants did not display the branched shoot phenotype observed in other SL‐defective mutants. At the protein level, SlG1 physically interacted with the root‐specific PSY3 isoform but not with PSY1 and PSY2. Our results confirm specific roles for SlG1 in producing GGPP for defensive diterpenoids in leaves and carotenoid‐derived SLs (in combination with PSY3) in roots.
An analysis-ready and quality controlled resource for pediatric brain white-matter research
Publisher: Springer Science and Business Media LLC
Date: 12-10-2022
DOI: 10.1038/S41597-022-01695-7
Abstract: We created a set of resources to enable research based on openly-available diffusion MRI (dMRI) data from the Healthy Brain Network (HBN) study. First, we curated the HBN dMRI data (N = 2747) into the Brain Imaging Data Structure and preprocessed it according to best-practices, including denoising and correcting for motion effects, susceptibility-related distortions, and eddy currents. Preprocessed, analysis-ready data was made openly available. Data quality plays a key role in the analysis of dMRI. To optimize QC and scale it to this large dataset, we trained a neural network through the combination of a small data subset scored by experts and a larger set scored by community scientists. The network performs QC highly concordant with that of experts on a held out set (ROC-AUC = 0.947). A further analysis of the neural network demonstrates that it relies on image features with relevance to QC. Altogether, this work both delivers resources to advance transdiagnostic research in brain connectivity and pediatric mental health, and establishes a novel paradigm for automated QC of large datasets.
Tomato geranylgeranyl diphosphate synthase isoform 1 specifically interacts with phytoene synthase isoform 3 to produce strigolactones in tomato roots
Publisher: Cold Spring Harbor Laboratory
Date: 02-11-2022
DOI: 10.1101/2022.11.01.514744
Abstract: Carotenoids are photoprotectant pigments and precursors of the hormones abscisic acid (ABA) and strigolactones (SL). Carotenoids are produced in plastids from geranylgeranyl diphosphate (GGPP), which is erted to the carotenoid pathway by phytoene synthase (PSY). In tomato ( Solanum lycopersicum ), 3 genes encode plastid-targeted GGPP synthases ( SlG1 to 3 ) and 3 genes encode PSY isoforms ( PSY1 to 3 ). Here we investigated the function of SlG1 by generating loss-of-function lines and combining their metabolic and physiological phenotypes with gene co-expression and co-immunoprecipitation analyses. Leaves and fruits of slg1 lines showed a wild-type phenotype in terms of isoprenoid accumulation, photosynthesis and development. Consistently, SlG1 is co-expressed with PSY3 and other genes involved in the production of carotenoids and SL (but not ABA) only in roots. SlG1 was also found to physically interact with the root-specific PSY3 isoform (and not with PSY1 and PSY2). Root SL (but not ABA) levels were reduced in slg1 lines. Our results confirm a specific role of SlG1 in SL production in combination with PSY3. This role appears to be restricted to roots as slg1 plants do not exhibit the shoot phenotype displayed by other SL-deficient mutants.
Related Organisations
University Of Manchester
Location: United Kingdom of Great Britain and Northern Ireland
Institute Of Molecular And Cellular Biology Of Plants
Location: Spain
Corporacion Colombiana De Investigación Agropecuaria
Location: Colombia
Related Funding Activities
No related grants have been discovered for Esteban Burbano-Erazo.