Design of Cas9 nucleases with reduced basal DNA binding and enhanced recombinase activity for human genome engineering

Funding Activity

Website
http://purl.org/au-research/grants/nhmrc/1090875

Funding Status
Closed

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Funded Activity Summary

Cas9 has recently emerged as a transformative tool for genome engineering. The enzyme is guided by a short RNA to pair with a DNA sequence of interest and to introduce a break. This DNA break becomes a substrate for repair pathways: Non-Homologous End-Joining or Homologous Recombination. The rate of HR is typically lower, limiting the efficiency of insertion of new DNA. The project aims to determine these reasons for limited rates of HR, and to improve these rates through protein engineering.

Funded Activity Details

Start Date: 01-01-2015

End Date: 01-01-2019

Funding Scheme: Early Career Fellowships

Funding Amount: $385,761.00

Funder: National Health and Medical Research Council

Research Topics

ANZSRC Field of Research (FoR)

Medical Molecular Engineering of Nucleic Acids and Proteins

ANZSRC Socio-Economic Objective (SEO)

There are no SEO codes available for this funding activity

Other Keywords

RNA binding proteins | genetic engineering | molecular evolution | protein engineering | targeted therapy

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