Validation and implementation of rapid test kits for detection of OsHV-1

Funding Activity

Website
https://www.frdc.com.au/project/2018-127

Funding Status
Closed

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Funded Activity Summary

Following the outbreak of Pacific Oyster Mortality Syndrome (POMS) in the Port River, South Australia in summer 2017-18, a need was identified for rapid diagnostic technology for OsHV-1, the causative agent of POMS. During the February 2016 OsHV-1 outbreak in Tasmania, tracing activities in Tasmania and South Australia required substantial follow-up and surveillance to define diseased areas and inform OsHV-1 management. This surveillance was slow and expensive, although it was observed that in outbreak situations OsHV-1 prevalence and viral load are high. The Australian Pacific oyster aquaculture industries and State governments have expressed a need for more cost effective surveillance options for monitoring disease in affected areas.

The advantage of a rapid test is that in the event of an oyster mortality, information would be available within hours, rather than days, to guide response measures. It is likely that field techniques will be less sensitive than laboratory methods, but OsHV-1 in outbreaks has characteristics that lend it to field based diagnostic methods including high prevalence and viral load and few diseases with similar presentation.

This project will review available technology, select a candidate approach, and implement an OsHV-1 test using the preferred platform. We will test samples in parallel with qPCR to assess the sensitivity and specificity of the rapid test platform.

Objectives:
1. Identify platform for rapid diagnostic test for OsHV-1
2. Implement OsHV-1 test in the chosen platform
3. Assess DSe and DSp of test in rapid platform
4. Provide guidelines and instructions for use

Funded Activity Details

Start Date: 30-11-2018

End Date: 30-06-2019

Funding Scheme: Funding Scheme not available

Funding Amount: $55,000.00

Funder: Fisheries Research and Development Corporation

Research Topics

ANZSRC Field of Research (FoR)

There are no FoR codes available for this funding activity

ANZSRC Socio-Economic Objective (SEO)

There are no SEO codes available for this funding activity

Other Keywords

Biosecurity | Cost | Diagnostic | Disease | Fisheries Management

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