Linkage Projects - Grant ID: LP150100689

Funded Activity Summary

In vitro expression of macrocyclic peptides. This project aims to develop a novel strategy for the production of polypeptides with unnatural chemical groups using a sense codon reassignment approach. Novel peptides could be used in a range of pharmaceutical applications. Peptides made of 20 natural amino acids cover only a very small fraction of the available chemical and functional space. While a peptide’s functionality can be extended with unnatural amino acids, the methods for their site-selective incorporation are inefficient. The project’s strategy relies on the depletion of selected tRNAs from an in vitro protein translation system and their replacement with synthetic tRNAs, charged with unnatural amino acids. It is expected that the developed technology could be used to rapidly generate and screen highly diversified macrocyclic peptide libraries.

Funded Activity Details

Start Date: 27-06-2016

End Date: 31-12-2020

Funding Scheme: Linkage Projects

Funding Amount: $670,242.00

Funder: Australian Research Council

Research Topics

ANZSRC Field of Research (FoR)

Enzymes | Biochemistry and Cell Biology | Industrial Molecular Engineering of Nucleic Acids and Proteins | Synthetic Biology |

ANZSRC Socio-Economic Objective (SEO)

Expanding Knowledge in the Biological Sciences | Health not elsewhere classified | Expanding Knowledge in Technology

Other Keywords

Biochemistry and Cell Biology | Enzymes | Expanding Knowledge in Technology | Expanding Knowledge in the Biological Sciences | Health not elsewhere classified | Industrial Molecular Engineering of Nucleic Acids and Proteins | Synthetic Biology

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