Discovery Projects - Grant ID: DP0343672

Funding Activity

Website
http://dataportal.arc.gov.au/NCGP/Web/Grant/Grant/DP0343672

Funding Status
Closed

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Funded Activity Summary

Engineering and expression of recombinant antibodies that interact with plant pathogenic phytoplasma membrane proteins - a model for phytoplasma disease management. . In Australia phytoplasmas are associated with serious diseases including papaya dieback and strawberry lethal yellows. Phytoplasmas cannot be cultured so there is a paucity of knowledge at the genomic level. We have identified the ABC Transporter and FtsH genes and expressed these phytoplasma membrane proteins (MP) in E. coli . We will generate antibody fragments that bind these MP and express them in tomato. We will analyse antibody expression in tomato and study their association with the phytoplasma membrane. Transgenic plants will be inoculated with phytoplasma and monitored for resistance. This work represents a major step towards engineering resistance to phytoplasmas.

Funded Activity Details

Start Date: 01-01-2003

End Date: 31-12-2007

Funding Scheme: Discovery Projects

Funding Amount: $290,000.00

Funder: Australian Research Council

Research Topics

ANZSRC Field of Research (FoR)

Microbial Genetics | Plant Biology | Genetic Technologies: Transformation, Site-Directed Mutagenesis, Etc. | Plant Pathology

ANZSRC Socio-Economic Objective (SEO)

Tropical fruit | Horticultural crops |

Other Keywords

Genetic Technologies: Transformation, Site-Directed Mutagenesis, Etc. | Horticultural crops | Microbial Genetics | Plant Biology | Plant Pathology | Tropical fruit

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