Visualising chromatin changes in 3 dimensions: super to ultra resolution. Packaging of genomic information into the nucleus of a cell necessitates the formation of tightly compacted and highly organized genomic structures within the nucleus, a configuration that is inherently repressive for gene transcription. Hence, mechanisms that alter the spatial organisation of DNA are critical to enable a variety of genome functions, including DNA transcription. This proposal will utilise novel adaptations ....Visualising chromatin changes in 3 dimensions: super to ultra resolution. Packaging of genomic information into the nucleus of a cell necessitates the formation of tightly compacted and highly organized genomic structures within the nucleus, a configuration that is inherently repressive for gene transcription. Hence, mechanisms that alter the spatial organisation of DNA are critical to enable a variety of genome functions, including DNA transcription. This proposal will utilise novel adaptations of super resolution microscopy to visualise in 3 dimensions how changes in chromatin modifications impact genome spatial organisation within the nucleus, and how this then links to cellular differentiation. This will provide a picture of how spatial organisation within the nucleus supports general cell differentiation.
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In depth characterisation of the gamma delta T cell immune synapse. This project aims to comprehensively characterise the activation principles of gamma delta T cells. These cells have an understudied but central role in vertebrate immunity and development. A missing piece of the puzzle is how gamma delta T cells sense stress and how this signal leads to activation. Expected outcomes include the generation of fundamental knowledge in immunology and structural biology. This proposal uses high-ski ....In depth characterisation of the gamma delta T cell immune synapse. This project aims to comprehensively characterise the activation principles of gamma delta T cells. These cells have an understudied but central role in vertebrate immunity and development. A missing piece of the puzzle is how gamma delta T cells sense stress and how this signal leads to activation. Expected outcomes include the generation of fundamental knowledge in immunology and structural biology. This proposal uses high-skilled techniques, including cryo-electron microscopy and single-molecule imaging and holds ancillary benefits to postgraduate students. Anticipated outcomes include influential publications, building a critical mass of expertise in Australia and fostering international collaborations with Australia at the epicentre.Read moreRead less
Discovering New Particle Physics with Dark Matter and Astrophysical Neutrinos. This project will place Australia at the forefront of pure basic research, and will forge connections with key international institutions. What is the Universe made of? How did it evolve? We address fundamental questions about our Universe, drawing on recent and forthcoming experimental data. We will contribute to Australia's skill base via the training of the best and brightest postgraduate students. These studen ....Discovering New Particle Physics with Dark Matter and Astrophysical Neutrinos. This project will place Australia at the forefront of pure basic research, and will forge connections with key international institutions. What is the Universe made of? How did it evolve? We address fundamental questions about our Universe, drawing on recent and forthcoming experimental data. We will contribute to Australia's skill base via the training of the best and brightest postgraduate students. These students will be equipped with the skills to act as original thinkers on general problems in the wider community. Fundamental questions about the nature of our Universe engage the intellectual curiosity of the general public and inspire the next generation of scientists.Read moreRead less