Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, ....Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, cytosolic pH and aluminium uptake across the plasma membrane in aluminium toxicity to plants. Knowledge of primary triggers of aluminium toxicity will pay off in a breeding programme aimed at selecting crop genotypes with increased resistance to aluminium toxicity.Read moreRead less
Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop culti ....Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop cultivars are important part of sustainable farming in Australia. This project will characterise early triggers of Al toxicity in plants, providing a foundation for increasing Al resistance in crop cultivars. Understanding the physiological basis of Al toxicity will lead to improved crop breeding strategies.Read moreRead less
IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the A ....IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the AMT family of ammonium transporters and associated signalling pathways which control the uptake and assimilation of ammonium in plants. This project will confirm the mechanisms involved in ammonium uptake from the soil and lead to the development of ammonium-nitrogen efficient crop plants.Read moreRead less
Fingerprinting the soil microbial metagenome. The understanding of the impact of current farming systems on soil biology is in its infancy. Technology previously used to examine soil biology only investigates a very small percentage of all soil organisms. We will use an innovative new technology (DArT) to rapidly gain an overview of all soil microbial biodiversity. We will then evaluate the impact of agricultural practices on that biodiversity, firstly based on our long term trial site exhibiti ....Fingerprinting the soil microbial metagenome. The understanding of the impact of current farming systems on soil biology is in its infancy. Technology previously used to examine soil biology only investigates a very small percentage of all soil organisms. We will use an innovative new technology (DArT) to rapidly gain an overview of all soil microbial biodiversity. We will then evaluate the impact of agricultural practices on that biodiversity, firstly based on our long term trial site exhibiting common farming practices, and then by a broader regional survey. Our longer term goal is to find microbiological indicators of healthy soil through a vastly improved ability to determine a wide range of beneficial and disease organisms to identify sustainable farming practices.Read moreRead less
Plant cell wall - aluminium interactions: a role in aluminium stress. Soil acidity, resulting in Al toxicity, affects production on circa 50% of Australia's intensively used agricultural land, resulting in 8.5 times more foregone agricultural income than dryland salinity (National Land and Water Resources Audit, 2001). Often, remediation with lime is not possible or affordable. By understanding Al reactions with cell wall components, we aim to identify root parameters related to Al resistance by ....Plant cell wall - aluminium interactions: a role in aluminium stress. Soil acidity, resulting in Al toxicity, affects production on circa 50% of Australia's intensively used agricultural land, resulting in 8.5 times more foregone agricultural income than dryland salinity (National Land and Water Resources Audit, 2001). Often, remediation with lime is not possible or affordable. By understanding Al reactions with cell wall components, we aim to identify root parameters related to Al resistance by plants. These parameters will be useful as selection markers to identify and breed Al-tolerant crop genotypes. This, in turn, will improve yields and farm profitability, allowing farmers to use lime to prevent further acidification. This increases sustainability of cropping operations on the 50 Mha with acid soils.Read moreRead less