Towards An Understanding Of Dynamic Genetic Regulation At A Global Scale In Bacteria: A Systems Biology Approach
Funder
National Health and Medical Research Council
Funding Amount
$486,000.00
Summary
To achieve major breakthroughs in the understanding of the regulation of gene transcription in bacteria at a global scale. High throughput technologies (living cell arrays, tiling DNA microarrays, multidimensional liquid chromatography proteomics and quantitative metabolomics) will be developed in conjunction with new computational modelling concepts to facilitate the understanding of biological complexity. The iterative process of simulations and model driven targeted experiments will generate ....To achieve major breakthroughs in the understanding of the regulation of gene transcription in bacteria at a global scale. High throughput technologies (living cell arrays, tiling DNA microarrays, multidimensional liquid chromatography proteomics and quantitative metabolomics) will be developed in conjunction with new computational modelling concepts to facilitate the understanding of biological complexity. The iterative process of simulations and model driven targeted experiments will generate novel hypotheses about the mechanistic nature of dynamic cellular responses, unravel emerging systems properties, and ultimately provide an efficient roadmap to tackle novel, pathogenic organisms.The role of my laboratory in this project is to perform subcellular localisation of transcription factors, establish standard operating procedures (with other groups) for growth, imaging and image analysis, precisely quantify 6 transcription factors under 3 standardised growth conditions for incorporation into metabolic models, and perform some analysis of transcription complexes.Read moreRead less
The Role Of Interferon-regulatory Factors In The Host Defense Against Bacterial Infection
Funder
National Health and Medical Research Council
Funding Amount
$355,711.00
Summary
Type I interferons are used in the treatment of viral infection. However, the therapeutic potential of type I interferons for the treatment of bacterial infection is not known because we do not fully understand their functional roles and regulation in hosts infected with bacteria. My proposal aims to investigate the role of one family of regulatory proteins, known as interferon-regulatory factors, in the host defense against foodborne bacteria.
Alphaherpesvirus recombination: safety implications for attenuated Herpesvirus vaccines. Under certain conditions some herpesviruses, including mild vaccine strains, can recombine to generate virulent viruses. Following findings that this occurred naturally between Australian poultry vaccines, with devastating results, this project will study natural herpesvirus recombination with the aim of allowing vaccines to be used more safely.
Alphaherpesvirus vaccination, recombination and latency; a study in the natural host. Attenuated alphaherpesvirus vaccines are used widely in production and companion animals to help control disease. These vaccines help to prevent clinical signs of disease following challenge with virulent viruses. There is also the potential to use these vaccines to help prevent latent herpesvirus infections, and to limit the opportunities for herpesvirus recombination to occur. This would enhance the ability t ....Alphaherpesvirus vaccination, recombination and latency; a study in the natural host. Attenuated alphaherpesvirus vaccines are used widely in production and companion animals to help control disease. These vaccines help to prevent clinical signs of disease following challenge with virulent viruses. There is also the potential to use these vaccines to help prevent latent herpesvirus infections, and to limit the opportunities for herpesvirus recombination to occur. This would enhance the ability to control disease in animal populations. This project aims to systematically study how vaccines may be used to limit latency and recombination events by studying avian infectious laryngotracheitis virus in the natural host leading to new insights into how vaccines may be used more efficaciously.Read moreRead less
Enhancing Control Of Enteric Bacteria Through Pathogen Genomics
Funder
National Health and Medical Research Council
Funding Amount
$645,205.00
Summary
Bacteria part of the Enterobacteriaceae family are responsible for causing significant enteric disease in Australia and internationally. Compounding the public health threat posed by these enteric bacteria is the rise in antimicrobial resistance, which limits treatment options. This project has three complementary research objectives; 1) to investigate new control strategies; 2) to better understand outbreak dynamics and; 3) to explore how bacteria are causing new disease in humans.
Understanding Complex Genomic And Biochemical Landscapes In Human Health Microbiology
Funder
National Health and Medical Research Council
Funding Amount
$2,210,520.00
Summary
There is increasing awareness of the critical roles microbes play in health. The microbes in our gut produce chemicals that control everything from our moods, to food metabolism and immune system function. In this proposal leading-edge technologies from the disciplines of microbiology, genomics, mass-spectrometry and computational biology will be used to reveal for the first time how and why these chemicals are produced by microbes in complex systems that are important for human health.
The Use Of Dosing Software With Bayesian Forecasting To Improve Antimicrobial-plasma Concentrations In The Intensive Care Unit
Funder
National Health and Medical Research Council
Funding Amount
$77,422.00
Summary
Emerging technologies such as DSBF is capable of assisting clinicians with selecting dosing regimens that achieve the target antimicrobial concentrations needed for treating infections. We will conduct a clinical trial to investigate the ability of DSBF to improve antimicrobial concentrations of patients in the ICU. The results of this study may lead to clinicians being given a new powerful validated tool for improving the treatment outcomes of patients suffering from infections in the ICU.
Early Career Industry Fellowships - Grant ID: IE230100594
Funder
Australian Research Council
Funding Amount
$447,914.00
Summary
Advancing chicken helminthology for sustainable worm control . Worm infections are a serious disease problem in free-range chicken production systems which now dominate egg production in Australia. This project aims to improve the control of worm infections of chickens by developing new tools for poultry researchers, advisors and farmers to use for this purpose. The project expects to develop novel methods for laboratory-based anthelmintic drug resistance testing, maintenance of defined worm str ....Advancing chicken helminthology for sustainable worm control . Worm infections are a serious disease problem in free-range chicken production systems which now dominate egg production in Australia. This project aims to improve the control of worm infections of chickens by developing new tools for poultry researchers, advisors and farmers to use for this purpose. The project expects to develop novel methods for laboratory-based anthelmintic drug resistance testing, maintenance of defined worm strains and diagnosis of infection. It will also determine the effectiveness of mass treatment in the field and the prevalence of anthelmintic resistance. Use of these tools and information will improve worm control and thus the productivity and welfare of free-range chickens in Australia and worldwide.Read moreRead less