Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of th ....Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of the host-pathogen interactions. A successful outcome could contribute an additional 5-20% increase in crop yields (depending on the crop) through inherent resistance of crops to nematode pests. This would benefit rural communities and the national economy, and could also generate international royalties.Read moreRead less
Field based molecular diagnostics for identification of plant parasitic nematodes. Nematodes are economically important pests of many agricultural and commercially grown plants. We have shown 'proof-of-concept' that plant parasitic nematodes can be identified by protein profiling using MALDI-TOF mass spectroscopy. In this project advanced techniques of proteomics and associated bioinformatics will be used to identify, isolate and characterise proteins that are specific to economically important ....Field based molecular diagnostics for identification of plant parasitic nematodes. Nematodes are economically important pests of many agricultural and commercially grown plants. We have shown 'proof-of-concept' that plant parasitic nematodes can be identified by protein profiling using MALDI-TOF mass spectroscopy. In this project advanced techniques of proteomics and associated bioinformatics will be used to identify, isolate and characterise proteins that are specific to economically important nematode species and races, and to identify diagnostic proteins or epitopes. The diagnostic proteins will be used to generate specific monoclonal antibodies that will be incorporated into immunochemical 'Lateral Flow' devices. These will provide on-site tests to identify nematodes for growers and quarantine services. Read moreRead less
Combinatorial controlled gene expression delivering crops resistant to nematodes. Root-knot nematodes cause US$130 billion crop losses worldwide pa, and at least AUS$ 450 pa in Australia. Current control methods involve fumigation, chemicals (mainly carbamates and organophosphates), natural plant resistance and biological control. The fumigants (eg methyl bromide) are being phased out because they damage the ozone layer, most of the non-fumigants are being banned because of environmental damag ....Combinatorial controlled gene expression delivering crops resistant to nematodes. Root-knot nematodes cause US$130 billion crop losses worldwide pa, and at least AUS$ 450 pa in Australia. Current control methods involve fumigation, chemicals (mainly carbamates and organophosphates), natural plant resistance and biological control. The fumigants (eg methyl bromide) are being phased out because they damage the ozone layer, most of the non-fumigants are being banned because of environmental damage and persistence in groundwater, and biological control has had limited success. These problems are addressed in this project with development of synthetic plant resistance to nematodes, which will benefit horticultural and broadacre farming by reducing pathogen losses and improving quality.Read moreRead less
Protecting the Australian chickpea industry through knowledge of the current Ascochyta rabiei fungal population and risk to resistance breeding strategies. Australian chickpea is highly vulnerable to epidemics of Ascochyta blight, which may cause total crop failure. This project will help to maintain Australia's position as a major global chickpea producer through maximising the life span of current resistance genes to A. rabiei. and determining the applicability of other potential resistance so ....Protecting the Australian chickpea industry through knowledge of the current Ascochyta rabiei fungal population and risk to resistance breeding strategies. Australian chickpea is highly vulnerable to epidemics of Ascochyta blight, which may cause total crop failure. This project will help to maintain Australia's position as a major global chickpea producer through maximising the life span of current resistance genes to A. rabiei. and determining the applicability of other potential resistance sources. The knowledge that will be generated regarding the pathogen's potential to overcome host resistance is imperative for developing future disease management strategies, especially since more aggressive isolates exist outside Australia. The project findings will feed directly into the National Australian Chickpea Breeding Program.Read moreRead less
Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants? It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basi ....Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants? It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basis of this discrepancy and to attempt to provide tools that circumvent the problem. It is expected the project will generate novel information on the environment in the plant experienced by the fungus.Read moreRead less
Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across sout ....Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across southern Australia. Benefits include prevention of severe losses in canola from downy mildew, and more viable and sustainable production with less reliance upon fungicides. This research addresses the National Research Priority 'An Environmentally Sustainable Australia' and the Priority Goal of 'Transforming existing industries', and will particularly benefit southern Australian rural communities.Read moreRead less