Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989105
Funder
Australian Research Council
Funding Amount
$495,000.00
Summary
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry. Biomolecular research and research training, in which proteomics is core, has become a critical component of post-industrial development in the Hunter region. Development of a cutting edge proteomics facility will benefit a research community comprising over 50 researchers and 150 undergraduate students significantly enhancing their research productivity and translation of outcomes in areas of national i ....An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry. Biomolecular research and research training, in which proteomics is core, has become a critical component of post-industrial development in the Hunter region. Development of a cutting edge proteomics facility will benefit a research community comprising over 50 researchers and 150 undergraduate students significantly enhancing their research productivity and translation of outcomes in areas of national importance. These include understanding the impact of the environment on plant and animal development, pest animal control, development of new biotechnology tools, new drugs and new methods for the detection of narcotics and explosives.Read moreRead less
Identification of Proteins that Regulate Apoptosis Through Interaction With IAPS. Apoptosis is the process by which multicellular organisms eliminate unwanted cells. Identifying proteins involved in cell death regulation is central to our understanding of disease states arising from aberrations in this process. The mammalian protein DIABLO, promotes cell death by interacting with and antagonising inhibitor of apoptosis proteins (IAPS). Given the existence of several IAP regulatory proteins (IRPs ....Identification of Proteins that Regulate Apoptosis Through Interaction With IAPS. Apoptosis is the process by which multicellular organisms eliminate unwanted cells. Identifying proteins involved in cell death regulation is central to our understanding of disease states arising from aberrations in this process. The mammalian protein DIABLO, promotes cell death by interacting with and antagonising inhibitor of apoptosis proteins (IAPS). Given the existence of several IAP regulatory proteins (IRPs) in insects, other mammalian IRPs probably also exist. These may be of equal importance in regulating apoptosis, especially in tissues where DIABLO is not expressed. The main aim of the proposed study is to idenitify and characterise other IRPs in mammalian cells.Read moreRead less
Function and modulation of the protein quality control network in mammalian mitochondria. This project has potential technological benefit in the areas of biotechnology and molecular medicine especially in relation to age-related cellular degeneration. As a result of our research outputs, strategies could be developed to either delay the onset or reduce the severity of diseases related to mitochondrial dysfunction. Training research scientists of the future, forms an integral part of our researc ....Function and modulation of the protein quality control network in mammalian mitochondria. This project has potential technological benefit in the areas of biotechnology and molecular medicine especially in relation to age-related cellular degeneration. As a result of our research outputs, strategies could be developed to either delay the onset or reduce the severity of diseases related to mitochondrial dysfunction. Training research scientists of the future, forms an integral part of our research program and our association with world leaders in the field provide excellent opportunity for exchange of personnel, ideas and emerging methodologies. This project will lead the way in this field and consequently will expand Australia's reputation at the forefront of scientific advancement. Read moreRead less
A proteomic approach to identifying the signaling pathway(s) by which acute oxidative stress causes cell death by apoptosis. Oxidative stress following traumatic injury (heart attack or stroke) is known to activate signaling pathways leading to programmed cell death (apoptosis). The aim of this project is to develop methods to identify the signaling proteins involved. Identifying proteins involved in causing cell death will be useful in developing diagnostic tools as well as providing potential ....A proteomic approach to identifying the signaling pathway(s) by which acute oxidative stress causes cell death by apoptosis. Oxidative stress following traumatic injury (heart attack or stroke) is known to activate signaling pathways leading to programmed cell death (apoptosis). The aim of this project is to develop methods to identify the signaling proteins involved. Identifying proteins involved in causing cell death will be useful in developing diagnostic tools as well as providing potential therapeutic possibilities.Read moreRead less
AAA+ proteases: substrate binding, translocation and modulation by novel adaptor proteins. Protein quality control is essential for the proper maintenance of the cell. It ensures the correct folding of newly synthesised proteins, the refolding or degradation of misfolded and aggregated proteins, and the controlled degradation of regulatory proteins. These functions are collectively performed by molecular chaperones and proteases. This project will define the molecular basis of substrate selectiv ....AAA+ proteases: substrate binding, translocation and modulation by novel adaptor proteins. Protein quality control is essential for the proper maintenance of the cell. It ensures the correct folding of newly synthesised proteins, the refolding or degradation of misfolded and aggregated proteins, and the controlled degradation of regulatory proteins. These functions are collectively performed by molecular chaperones and proteases. This project will define the molecular basis of substrate selectivity for ATP-dependent proteases and determine the relationship between chaperones and proteases. A major focus will be directed towards the mechanistic analysis of novel AAA+ cofactors such as ClpS, which we recently discovered. A detailed analysis of such proteins is central to understanding how chaperones and protease (a) recognize their substrates and (b) compete for different substrates in vivo.Read moreRead less
O-GlcNAc-phosphorylation: a novel post-translational modification regulating vesicle recycling. We will determine a biological role for our discovery of a hybrid protein modification (both carbohydrate and phosphate) on a brain protein that is involved in nerve cell communication. If this modification is more widespread, then we will have discovered a new level of cellular regulation. This discovery is likely to have a broad benefit. It will advance the understanding of carbohydrate and phosphat ....O-GlcNAc-phosphorylation: a novel post-translational modification regulating vesicle recycling. We will determine a biological role for our discovery of a hybrid protein modification (both carbohydrate and phosphate) on a brain protein that is involved in nerve cell communication. If this modification is more widespread, then we will have discovered a new level of cellular regulation. This discovery is likely to have a broad benefit. It will advance the understanding of carbohydrate and phosphate modified proteins. For example, there may be consequences for the model of hyperphosphorylated and carbohydrate modified proteins involved in neurodegeneration. There will also be a targeted benefit. An improved understanding of the mechanism of neurotransmission will benefit in designing compounds to fight diseases of neurotransmission.Read moreRead less