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Scheme : NHMRC Project Grants
Research Topic : targeted calcium sensors
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  • Funded Activity

    Regulation Of Nuclear Calcium Concentration In The Life Or Death Of Cells

    Funder
    National Health and Medical Research Council
    Funding Amount
    $195,047.00
    Summary
    The nucleus is the most prominent of all cell organelles and contains the primary genetic material for cellular development and growth. It performs some of the most important functions in the life and death of all living cells. However, little is known about many of the regulatory signals and events that control nuclear function. We will use new genetically-encoded sensor molecules that a living cell can be instructed to produce at various internal locations to explore important features of cell .... The nucleus is the most prominent of all cell organelles and contains the primary genetic material for cellular development and growth. It performs some of the most important functions in the life and death of all living cells. However, little is known about many of the regulatory signals and events that control nuclear function. We will use new genetically-encoded sensor molecules that a living cell can be instructed to produce at various internal locations to explore important features of cell control. This study will look specifically at how changes in the concentration of ionised Ca2+ in the nucleus control the switching on of genes and the initiation of programmed cell death pathways. This information is of significance to our understanding of normal cell growth and development, as well as abnormal growth (e.g. cancer).
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    Calcium Influx Pathways And Breast Cancer

    Funder
    National Health and Medical Research Council
    Funding Amount
    $398,352.00
    Summary
    Calcium acts as a signal to control cell processes important in cancer. The entry of calcium into the cell is regulated by calcium channels and we have found some channels are over-expressed in breast cancer. Altering the expression and activity of these calcium channels is a possible therapeutic approach for cancer. We will determine the reasons and consequences of alterations of calcium channels in breast cancer and whether they are viable anti-cancer therapies and biomarkers.
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    Funded Activity

    The Stunned Myocardium

    Funder
    National Health and Medical Research Council
    Funding Amount
    $567,728.00
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    Two Recently Identified Calcium Transporters In Lactation And During Mammary Epithelial Cell Apoptosis.

    Funder
    National Health and Medical Research Council
    Funding Amount
    $465,115.00
    Summary
    The transport of calcium into milk is a key event in human health. In addition to its importance in neonatal nutrition, the way breast cells regulate calcium also has implications in breast cancer, as well as the modification of proteins important in immunity, and the activity of biopharmaceuticals. This grant will determine how two specific calcium transporters regulate calcium levels in the breast and their respective roles in cellular processes important in normal function and in disease.
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    The Effect Of Ischaemia And Reperfusion On Sarcoplasmic Reticulum Calcium Handling In The Heart

    Funder
    National Health and Medical Research Council
    Funding Amount
    $236,208.00
    Summary
    Ischaemic heart disease is one of the most common causes of premature death in our society. Ischaemia occurs when the blood flow to the heart is obstructed so that oxygen cannot get to the muscle cells and metabolic waste products cannot be washed away. During ischaemia the concentration of free calcium within a cardiac muscle cell increases, and when blood flow is returned to the muscle this calcium concentration can increase further to very high levels. It is this change in calcium that is res .... Ischaemic heart disease is one of the most common causes of premature death in our society. Ischaemia occurs when the blood flow to the heart is obstructed so that oxygen cannot get to the muscle cells and metabolic waste products cannot be washed away. During ischaemia the concentration of free calcium within a cardiac muscle cell increases, and when blood flow is returned to the muscle this calcium concentration can increase further to very high levels. It is this change in calcium that is responsible for the reduced muscle force and abnormal cardiac rhythm that are the main cause of death. Cardiac muscle cells contain an intracellular compartment called the sarcoplasmic reticulum (SR). Under normal conditions the SR stores large amounts of calcium in order to maintain a low concentration of calcium free within the cell. However, even in a resting cell, calcium can escape from the SR through channels in SR membrane. We are using a state-of-the-art microscope to visualize these tiny packets of calcium, termed calcium sparks, as they travel through the SR membrane. If the number of calcium sparks increases, the amount of calcium being released from the SR also increases. We are studying what happens to calcium sparks, and therefore SR calcium release, during ischaemic heart disease. We are also examining the effect of ischaemic heart disease on the concentration of calcium within the SR and the activity of the transporters that pump calcium back into the SR. We hope to show that a change in the way the SR regulates calcium contributes to ischaemic damage. Understanding how changes in SR function alter muscle force and cardiac rhythm will help in the development of drugs to protect against ischaemic damage.
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    Funded Activity

    Calcium Handling Properties Of Diseased Muscle

    Funder
    National Health and Medical Research Council
    Funding Amount
    $149,516.00
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    Funded Activity

    Characterisation Of A Novel Amorphous Calcium Fluoride Phosphate Stabilised By Phosphopeptides

    Funder
    National Health and Medical Research Council
    Funding Amount
    $377,151.00
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    Funded Activity

    The Role Of The Cytoskeleton In Communication Between The L-type Ca2+ Channel And The Mitochondria In Cardiac Pathology

    Funder
    National Health and Medical Research Council
    Funding Amount
    $542,890.00
    Summary
    The L-type calcium channel is a protein in the membrane of heart muscle cells responsible for maintaining normal rhythm and contraction. We have shown that the channel can also regulate the function of the energy producing part of the cell (mitochondria). This occurs with the assistance of proteins that maintain cell architecture. We will test whether this association is altered in human disease where the cell architecture is disrupted to determine the mechanisms for poor energy supply.
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    Funded Activity

    The Role Of Calcium In The Control Of Red Blood Cells

    Funder
    National Health and Medical Research Council
    Funding Amount
    $394,516.00
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    Funded Activity

    Calcium Cycling Within Muscle Cells

    Funder
    National Health and Medical Research Council
    Funding Amount
    $141,487.00
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    Showing 1-10 of 222 Funded Activites

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